ABNORMAL HEMOGLOBINS: LABORATORY METHODS
Laboratory methods allowing the detection and characterization of hemoglobin variants are reviewed. Protein chemistry techniques such as isoelectrofocusing, electrophoreses under various experimental conditions, cation exchange and reversed phase high performance liquid chromatography, are the most...
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| Veröffentlicht in: | Hemoglobin 2001-01, Vol.25 (2), p.169-181 |
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| container_end_page | 181 |
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| container_issue | 2 |
| container_start_page | 169 |
| container_title | Hemoglobin |
| container_volume | 25 |
| creator | Wajcman, Henri Préhu, Claude Bardakdjian-Michau, Josiane Promé, Danielle Riou, Jean Godart, Christian Mathis, Mireille Hurtrel, Didier Galactéros, Frederic |
| description | Laboratory methods allowing the detection and characterization of hemoglobin variants are reviewed. Protein chemistry techniques such as isoelectrofocusing, electrophoreses under various experimental conditions, cation exchange and reversed phase high performance liquid chromatography, are the most frequently used for the detection of variants. When associated with a few additional data they may lead to a presumptive diagnosis. DNA studies are also developed in many laboratories. Final identi(r)cation of a variant may be achieved either by molecular biology techniques or by protein sequence analysis in which mass spectrometry now occupies a key position. |
| doi_str_mv | 10.1081/HEM-100104026 |
| format | Article |
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Protein chemistry techniques such as isoelectrofocusing, electrophoreses under various experimental conditions, cation exchange and reversed phase high performance liquid chromatography, are the most frequently used for the detection of variants. When associated with a few additional data they may lead to a presumptive diagnosis. DNA studies are also developed in many laboratories. Final identi(r)cation of a variant may be achieved either by molecular biology techniques or by protein sequence analysis in which mass spectrometry now occupies a key position.</description><identifier>ISSN: 0363-0269</identifier><identifier>EISSN: 1532-432X</identifier><identifier>DOI: 10.1081/HEM-100104026</identifier><identifier>PMID: 11480779</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Chemistry Techniques, Analytical - methods ; Clinical Laboratory Techniques - instrumentation ; Genetic Variation ; Hemoglobins, Abnormal - analysis ; Hemoglobins, Abnormal - genetics ; Humans ; Sequence Analysis, DNA ; Sequence Analysis, Protein - methods</subject><ispartof>Hemoglobin, 2001-01, Vol.25 (2), p.169-181</ispartof><rights>2001 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-953fa1790d1097b29725b454e44fc998490b06bf794b63a91119c3dd2594e14b3</citedby><cites>FETCH-LOGICAL-c386t-953fa1790d1097b29725b454e44fc998490b06bf794b63a91119c3dd2594e14b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1081/HEM-100104026$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1081/HEM-100104026$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11480779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wajcman, Henri</creatorcontrib><creatorcontrib>Préhu, Claude</creatorcontrib><creatorcontrib>Bardakdjian-Michau, Josiane</creatorcontrib><creatorcontrib>Promé, Danielle</creatorcontrib><creatorcontrib>Riou, Jean</creatorcontrib><creatorcontrib>Godart, Christian</creatorcontrib><creatorcontrib>Mathis, Mireille</creatorcontrib><creatorcontrib>Hurtrel, Didier</creatorcontrib><creatorcontrib>Galactéros, Frederic</creatorcontrib><title>ABNORMAL HEMOGLOBINS: LABORATORY METHODS</title><title>Hemoglobin</title><addtitle>Hemoglobin</addtitle><description>Laboratory methods allowing the detection and characterization of hemoglobin variants are reviewed. Protein chemistry techniques such as isoelectrofocusing, electrophoreses under various experimental conditions, cation exchange and reversed phase high performance liquid chromatography, are the most frequently used for the detection of variants. When associated with a few additional data they may lead to a presumptive diagnosis. DNA studies are also developed in many laboratories. Final identi(r)cation of a variant may be achieved either by molecular biology techniques or by protein sequence analysis in which mass spectrometry now occupies a key position.</description><subject>Chemistry Techniques, Analytical - methods</subject><subject>Clinical Laboratory Techniques - instrumentation</subject><subject>Genetic Variation</subject><subject>Hemoglobins, Abnormal - analysis</subject><subject>Hemoglobins, Abnormal - genetics</subject><subject>Humans</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Analysis, Protein - methods</subject><issn>0363-0269</issn><issn>1532-432X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFPwjAUhxujEUSPXg0n42Xat3br6m0gAslgCWCip6bbujCyMWy3GP57ayAaD5xe8vK9X97vQ-gW8CPgAJ4mo5kDGAOm2PXPUBc84jqUuO_nqIuJTxy75h10ZczGUpxheok6ADTAjPEueggH83gxC6O-DYrHUTyYzpfP_SgcxItwFS8--rPRahK_LK_RRS5Lo26Os4feXker4cSJ4vF0GEZOSgK_cbhHcgmM4wwwZ4nLmesl1KOK0jzlPKAcJ9hPcsZp4hPJAYCnJMtcj1MFNCE9dH_I3en6s1WmEVVhUlWWcqvq1ggG2HNtcws6BzDVtTFa5WKni0rqvQAsftQI20j8qrH83TG4TSqV_dFHFxYIDkCxzWtdya9al5lo5L6sda7lNi2MIKey2b_TtZJls06lVmJTt3prhZ346hsfeHxk</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Wajcman, Henri</creator><creator>Préhu, Claude</creator><creator>Bardakdjian-Michau, Josiane</creator><creator>Promé, Danielle</creator><creator>Riou, Jean</creator><creator>Godart, Christian</creator><creator>Mathis, Mireille</creator><creator>Hurtrel, Didier</creator><creator>Galactéros, Frederic</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>ABNORMAL HEMOGLOBINS: LABORATORY METHODS</title><author>Wajcman, Henri ; Préhu, Claude ; Bardakdjian-Michau, Josiane ; Promé, Danielle ; Riou, Jean ; Godart, Christian ; Mathis, Mireille ; Hurtrel, Didier ; Galactéros, Frederic</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-953fa1790d1097b29725b454e44fc998490b06bf794b63a91119c3dd2594e14b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Chemistry Techniques, Analytical - methods</topic><topic>Clinical Laboratory Techniques - instrumentation</topic><topic>Genetic Variation</topic><topic>Hemoglobins, Abnormal - analysis</topic><topic>Hemoglobins, Abnormal - genetics</topic><topic>Humans</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Analysis, Protein - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wajcman, Henri</creatorcontrib><creatorcontrib>Préhu, Claude</creatorcontrib><creatorcontrib>Bardakdjian-Michau, Josiane</creatorcontrib><creatorcontrib>Promé, Danielle</creatorcontrib><creatorcontrib>Riou, Jean</creatorcontrib><creatorcontrib>Godart, Christian</creatorcontrib><creatorcontrib>Mathis, Mireille</creatorcontrib><creatorcontrib>Hurtrel, Didier</creatorcontrib><creatorcontrib>Galactéros, Frederic</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hemoglobin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wajcman, Henri</au><au>Préhu, Claude</au><au>Bardakdjian-Michau, Josiane</au><au>Promé, Danielle</au><au>Riou, Jean</au><au>Godart, Christian</au><au>Mathis, Mireille</au><au>Hurtrel, Didier</au><au>Galactéros, Frederic</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ABNORMAL HEMOGLOBINS: LABORATORY METHODS</atitle><jtitle>Hemoglobin</jtitle><addtitle>Hemoglobin</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>25</volume><issue>2</issue><spage>169</spage><epage>181</epage><pages>169-181</pages><issn>0363-0269</issn><eissn>1532-432X</eissn><abstract>Laboratory methods allowing the detection and characterization of hemoglobin variants are reviewed. 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| fulltext | fulltext |
| identifier | ISSN: 0363-0269 |
| ispartof | Hemoglobin, 2001-01, Vol.25 (2), p.169-181 |
| issn | 0363-0269 1532-432X |
| language | eng |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Chemistry Techniques, Analytical - methods Clinical Laboratory Techniques - instrumentation Genetic Variation Hemoglobins, Abnormal - analysis Hemoglobins, Abnormal - genetics Humans Sequence Analysis, DNA Sequence Analysis, Protein - methods |
| title | ABNORMAL HEMOGLOBINS: LABORATORY METHODS |
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