Localization of Active Form of Caspase-8 in Mouse L929 Cells Induced by TNF Treatment and Polyglutamine Aggregates
The relation between activation of caspase-8 and polyglutamine aggregates has been focused. We prepared an antiserum (anti-m8D387) that recognizes the active form but not the proform of mouse caspase-8. We used immunostaining with anti-m8D387 antiserum to compare the localizations of activated mcasp...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-04, Vol.270 (3), p.972-977 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kouroku, Yoriko Fujita, Eriko Jimbo, Atushi Mukasa, Takeshi Tsuru, Tomohiko Momoi, Mariko Y. Momoi, Takashi |
| description | The relation between activation of caspase-8 and polyglutamine aggregates has been focused. We prepared an antiserum (anti-m8D387) that recognizes the active form but not the proform of mouse caspase-8. We used immunostaining with anti-m8D387 antiserum to compare the localizations of activated mcaspase-8 in L929 (clone 1422) cells induced by TNF and polyglutamine aggregates. Anti-m8D387 was positive throughout cytoplasm of the TUNEL-positive cells induced by TNF treatment, whereas the anti-m8D387 reactivity was not positive throughout cytoplasm of the cells expressing polyglutamine but was restricted to polyglutamine aggregates. In contrast with TNF-treated cells, cells expressing anti-m8D387-positive cytoplasmic polyglutamine aggregates did not undergo TUNEL-positive apoptosis. Thus activated caspase-8 associated with polyglutamine aggregates alone was not sufficient to induce TUNEL-positive apoptosis of L929 (clone 1422) cells. The distribution of activated caspase-8 associated with polyglutamine aggregates may be essential for the polyglutamine-mediated cell death or downstream of caspase-8 may be different in the TNF-treated cells and cells expressing polyglutamine. |
| doi_str_mv | 10.1006/bbrc.2000.2463 |
| format | Article |
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We prepared an antiserum (anti-m8D387) that recognizes the active form but not the proform of mouse caspase-8. We used immunostaining with anti-m8D387 antiserum to compare the localizations of activated mcaspase-8 in L929 (clone 1422) cells induced by TNF and polyglutamine aggregates. Anti-m8D387 was positive throughout cytoplasm of the TUNEL-positive cells induced by TNF treatment, whereas the anti-m8D387 reactivity was not positive throughout cytoplasm of the cells expressing polyglutamine but was restricted to polyglutamine aggregates. In contrast with TNF-treated cells, cells expressing anti-m8D387-positive cytoplasmic polyglutamine aggregates did not undergo TUNEL-positive apoptosis. Thus activated caspase-8 associated with polyglutamine aggregates alone was not sufficient to induce TUNEL-positive apoptosis of L929 (clone 1422) cells. The distribution of activated caspase-8 associated with polyglutamine aggregates may be essential for the polyglutamine-mediated cell death or downstream of caspase-8 may be different in the TNF-treated cells and cells expressing polyglutamine.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2000.2463</identifier><identifier>PMID: 10772935</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Caspase 8 ; Caspase 9 ; Caspases - analysis ; Caspases - metabolism ; COS Cells ; Fibrosarcoma ; Green Fluorescent Proteins ; Luminescent Proteins - analysis ; Mice ; Peptides - analysis ; Peptides - metabolism ; Recombinant Fusion Proteins - analysis ; Recombinant Fusion Proteins - metabolism ; Transfection ; Tumor Cells, Cultured ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 2000-04, Vol.270 (3), p.972-977</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-9b4c80b15aa490df425cdbc4b6ce33eeeed6d3a63e88fb64de348ff37a1727373</citedby><cites>FETCH-LOGICAL-c406t-9b4c80b15aa490df425cdbc4b6ce33eeeed6d3a63e88fb64de348ff37a1727373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.2000.2463$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10772935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kouroku, Yoriko</creatorcontrib><creatorcontrib>Fujita, Eriko</creatorcontrib><creatorcontrib>Jimbo, Atushi</creatorcontrib><creatorcontrib>Mukasa, Takeshi</creatorcontrib><creatorcontrib>Tsuru, Tomohiko</creatorcontrib><creatorcontrib>Momoi, Mariko Y.</creatorcontrib><creatorcontrib>Momoi, Takashi</creatorcontrib><title>Localization of Active Form of Caspase-8 in Mouse L929 Cells Induced by TNF Treatment and Polyglutamine Aggregates</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The relation between activation of caspase-8 and polyglutamine aggregates has been focused. 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The distribution of activated caspase-8 associated with polyglutamine aggregates may be essential for the polyglutamine-mediated cell death or downstream of caspase-8 may be different in the TNF-treated cells and cells expressing polyglutamine.</description><subject>Animals</subject><subject>Caspase 8</subject><subject>Caspase 9</subject><subject>Caspases - analysis</subject><subject>Caspases - metabolism</subject><subject>COS Cells</subject><subject>Fibrosarcoma</subject><subject>Green Fluorescent Proteins</subject><subject>Luminescent Proteins - analysis</subject><subject>Mice</subject><subject>Peptides - analysis</subject><subject>Peptides - metabolism</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDuPEzEURi0EYsNCS4lc0U24fsyrjCICK4VHESQ6y487kdHMONielcKvx6NsQcNtrGsdf_J3CHnLYMsAmg_GRLvlALDlshHPyIZBDxVnIJ-TTbluKt6zn3fkVUq_ABiTTf-S3DFoW96LekPiMVg9-j86-zDTMNCdzf4R6SHEaV33Ol10wqqjfqZfwpKQHnve0z2OY6IPs1ssOmqu9PT1QE8RdZ5wzlTPjn4P4_U8LllPfka6O58jnnXG9Jq8GPSY8M3TeU9-HD6e9p-r47dPD_vdsbISmlz1RtoODKu1lj24QfLaOmOlaSwKgWVc44RuBHbdYBrpUMhuGESrWctb0Yp78v6We4nh94Ipq8knW_6tZyxFVMughpp3BdzeQBtDShEHdYl-0vGqGKjVslotq9WyWi2XB--ekhczofsHv2ktQHcDsPR79BhVsh7nospHtFm54P-X_RcfSYuu</recordid><startdate>20000421</startdate><enddate>20000421</enddate><creator>Kouroku, Yoriko</creator><creator>Fujita, Eriko</creator><creator>Jimbo, Atushi</creator><creator>Mukasa, Takeshi</creator><creator>Tsuru, Tomohiko</creator><creator>Momoi, Mariko Y.</creator><creator>Momoi, Takashi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000421</creationdate><title>Localization of Active Form of Caspase-8 in Mouse L929 Cells Induced by TNF Treatment and Polyglutamine Aggregates</title><author>Kouroku, Yoriko ; Fujita, Eriko ; Jimbo, Atushi ; Mukasa, Takeshi ; Tsuru, Tomohiko ; Momoi, Mariko Y. ; Momoi, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-9b4c80b15aa490df425cdbc4b6ce33eeeed6d3a63e88fb64de348ff37a1727373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Caspase 8</topic><topic>Caspase 9</topic><topic>Caspases - analysis</topic><topic>Caspases - metabolism</topic><topic>COS Cells</topic><topic>Fibrosarcoma</topic><topic>Green Fluorescent Proteins</topic><topic>Luminescent Proteins - analysis</topic><topic>Mice</topic><topic>Peptides - analysis</topic><topic>Peptides - metabolism</topic><topic>Recombinant Fusion Proteins - analysis</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kouroku, Yoriko</creatorcontrib><creatorcontrib>Fujita, Eriko</creatorcontrib><creatorcontrib>Jimbo, Atushi</creatorcontrib><creatorcontrib>Mukasa, Takeshi</creatorcontrib><creatorcontrib>Tsuru, Tomohiko</creatorcontrib><creatorcontrib>Momoi, Mariko Y.</creatorcontrib><creatorcontrib>Momoi, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kouroku, Yoriko</au><au>Fujita, Eriko</au><au>Jimbo, Atushi</au><au>Mukasa, Takeshi</au><au>Tsuru, Tomohiko</au><au>Momoi, Mariko Y.</au><au>Momoi, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of Active Form of Caspase-8 in Mouse L929 Cells Induced by TNF Treatment and Polyglutamine Aggregates</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-04-21</date><risdate>2000</risdate><volume>270</volume><issue>3</issue><spage>972</spage><epage>977</epage><pages>972-977</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The relation between activation of caspase-8 and polyglutamine aggregates has been focused. We prepared an antiserum (anti-m8D387) that recognizes the active form but not the proform of mouse caspase-8. We used immunostaining with anti-m8D387 antiserum to compare the localizations of activated mcaspase-8 in L929 (clone 1422) cells induced by TNF and polyglutamine aggregates. Anti-m8D387 was positive throughout cytoplasm of the TUNEL-positive cells induced by TNF treatment, whereas the anti-m8D387 reactivity was not positive throughout cytoplasm of the cells expressing polyglutamine but was restricted to polyglutamine aggregates. In contrast with TNF-treated cells, cells expressing anti-m8D387-positive cytoplasmic polyglutamine aggregates did not undergo TUNEL-positive apoptosis. Thus activated caspase-8 associated with polyglutamine aggregates alone was not sufficient to induce TUNEL-positive apoptosis of L929 (clone 1422) cells. 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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Caspase 8 Caspase 9 Caspases - analysis Caspases - metabolism COS Cells Fibrosarcoma Green Fluorescent Proteins Luminescent Proteins - analysis Mice Peptides - analysis Peptides - metabolism Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - metabolism Transfection Tumor Cells, Cultured Tumor Necrosis Factor-alpha - pharmacology |
| title | Localization of Active Form of Caspase-8 in Mouse L929 Cells Induced by TNF Treatment and Polyglutamine Aggregates |
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