Humanization and characterization of the anti‐HLA‐DR antibody 1D10

1D10 is a previously described antibody that binds to cells from a majority of B‐cell malignancies. The current studies were designed to further evaluate the antigen specificity of 1D10 and its potential as an immunotherapeutic agent. Studies with transfectants and immunoprecipitation demonstrated t...

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Veröffentlicht in:International journal of cancer 2001-08, Vol.93 (4), p.556-565
Hauptverfasser: Kostelny, Sheri A., Link, Brian K., Tso, J. Yun, Vasquez, Max, Jorgensen, Brett H., Wang, Hong, Hall, William C., Weiner, George J.
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container_end_page 565
container_issue 4
container_start_page 556
container_title International journal of cancer
container_volume 93
creator Kostelny, Sheri A.
Link, Brian K.
Tso, J. Yun
Vasquez, Max
Jorgensen, Brett H.
Wang, Hong
Hall, William C.
Weiner, George J.
description 1D10 is a previously described antibody that binds to cells from a majority of B‐cell malignancies. The current studies were designed to further evaluate the antigen specificity of 1D10 and its potential as an immunotherapeutic agent. Studies with transfectants and immunoprecipitation demonstrated that 1D10 recognizes some, but not all, of the human HLA‐DR β chains. Both normal and malignant B cells can express the 1D10 antigen. A humanized version of 1D10 was produced using CDR grafting. The resulting antibody has an affinity that is similar to that of the parental murine antibody. In addition, the humanized antibody is capable of inducing complement‐mediated cytotoxicity, antibody‐dependent cell cytotoxicity, and direct apoptosis of 1D10‐expressing B cells. Based on these in vitro anti‐tumor activities, we conclude humanized 1D10 deserves further evaluation as an immunotherapeutic agent. © 2001 Wiley‐Liss, Inc.
doi_str_mv 10.1002/ijc.1366
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Yun ; Vasquez, Max ; Jorgensen, Brett H. ; Wang, Hong ; Hall, William C. ; Weiner, George J.</creator><creatorcontrib>Kostelny, Sheri A. ; Link, Brian K. ; Tso, J. Yun ; Vasquez, Max ; Jorgensen, Brett H. ; Wang, Hong ; Hall, William C. ; Weiner, George J.</creatorcontrib><description>1D10 is a previously described antibody that binds to cells from a majority of B‐cell malignancies. The current studies were designed to further evaluate the antigen specificity of 1D10 and its potential as an immunotherapeutic agent. Studies with transfectants and immunoprecipitation demonstrated that 1D10 recognizes some, but not all, of the human HLA‐DR β chains. Both normal and malignant B cells can express the 1D10 antigen. A humanized version of 1D10 was produced using CDR grafting. The resulting antibody has an affinity that is similar to that of the parental murine antibody. In addition, the humanized antibody is capable of inducing complement‐mediated cytotoxicity, antibody‐dependent cell cytotoxicity, and direct apoptosis of 1D10‐expressing B cells. Based on these in vitro anti‐tumor activities, we conclude humanized 1D10 deserves further evaluation as an immunotherapeutic agent. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.1366</identifier><identifier>PMID: 11477560</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: John Wiley &amp; Sons, Inc</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - genetics ; Antibodies, Monoclonal - immunology ; Antibody Affinity ; Antibody Specificity ; Antibody-Dependent Cell Cytotoxicity ; Antineoplastic agents ; Apoptosis - immunology ; Biological and medical sciences ; Cloning, Molecular ; Epitopes - immunology ; histocompatibility antigen HLA ; HLA-DR Antigens - immunology ; HLA‐DR ; Humans ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - genetics ; Immunoglobulin G - immunology ; Immunotherapy ; Leukemia, T-Cell - immunology ; Leukemia, T-Cell - pathology ; lymphoma ; Lymphoma, B-Cell - immunology ; Lymphoma, B-Cell - pathology ; Medical sciences ; MHC class II ; Mice ; Molecular Sequence Data ; monoclonal antibody ; Pharmacology. 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Yun</creatorcontrib><creatorcontrib>Vasquez, Max</creatorcontrib><creatorcontrib>Jorgensen, Brett H.</creatorcontrib><creatorcontrib>Wang, Hong</creatorcontrib><creatorcontrib>Hall, William C.</creatorcontrib><creatorcontrib>Weiner, George J.</creatorcontrib><title>Humanization and characterization of the anti‐HLA‐DR antibody 1D10</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>1D10 is a previously described antibody that binds to cells from a majority of B‐cell malignancies. The current studies were designed to further evaluate the antigen specificity of 1D10 and its potential as an immunotherapeutic agent. Studies with transfectants and immunoprecipitation demonstrated that 1D10 recognizes some, but not all, of the human HLA‐DR β chains. Both normal and malignant B cells can express the 1D10 antigen. A humanized version of 1D10 was produced using CDR grafting. The resulting antibody has an affinity that is similar to that of the parental murine antibody. In addition, the humanized antibody is capable of inducing complement‐mediated cytotoxicity, antibody‐dependent cell cytotoxicity, and direct apoptosis of 1D10‐expressing B cells. 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subjects Amino Acid Sequence
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - genetics
Antibodies, Monoclonal - immunology
Antibody Affinity
Antibody Specificity
Antibody-Dependent Cell Cytotoxicity
Antineoplastic agents
Apoptosis - immunology
Biological and medical sciences
Cloning, Molecular
Epitopes - immunology
histocompatibility antigen HLA
HLA-DR Antigens - immunology
HLA‐DR
Humans
Immunoglobulin G - biosynthesis
Immunoglobulin G - genetics
Immunoglobulin G - immunology
Immunotherapy
Leukemia, T-Cell - immunology
Leukemia, T-Cell - pathology
lymphoma
Lymphoma, B-Cell - immunology
Lymphoma, B-Cell - pathology
Medical sciences
MHC class II
Mice
Molecular Sequence Data
monoclonal antibody
Pharmacology. Drug treatments
Sequence Homology, Amino Acid
Tumor Cells, Cultured
title Humanization and characterization of the anti‐HLA‐DR antibody 1D10
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