Comparison among Differential Pulse Voltammetry, Amperometric Biosensor, and HPLC/DAD Analysis for Polyphenol Determination
Polyphenols are widespread in vegetables and fruits. They can play an important role in human diet and health, and they influence the sensorial properties of many foods, and act as natural antioxidants. This study was conducted using HPLC/DAD, tyrosinase biosensor, and differential pulse voltammetry...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2000-04, Vol.48 (4), p.1197-1203 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Romani, A Minunni, M Mulinacci, N Pinelli, P Vincieri, F. F Del Carlo, M Mascini, M |
| description | Polyphenols are widespread in vegetables and fruits. They can play an important role in human diet and health, and they influence the sensorial properties of many foods, and act as natural antioxidants. This study was conducted using HPLC/DAD, tyrosinase biosensor, and differential pulse voltammetry (DPV) analyses to detect polyphenolic compounds in natural complex matrices. The analyses were applied to a series of both standards and natural extracts derived from grape, olives, and green tea. The pure compounds include phenolic acids, flavones, flavonols, catechins, tannins, and oleuropein. HPLC/DAD, DPV, and the biosensor approach were used as independent analytical techniques. Bare graphite screen-printed electrodes were employed in DPV and in the biosensor analysis. The most accurate data were obtained by HPLC/DAD analysis, while the DPV approach using screen-printed electrodes could represent a quick screening method for the determination of polyphenols in natural extracts. Use of the biosensor for the analysis of complex matrices needs further study in order to improve its performance. Keywords: Polyphenols; anthocyanins; green tea; EGCG; olives; grapes |
| doi_str_mv | 10.1021/jf990767e |
| format | Article |
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The most accurate data were obtained by HPLC/DAD analysis, while the DPV approach using screen-printed electrodes could represent a quick screening method for the determination of polyphenols in natural extracts. Use of the biosensor for the analysis of complex matrices needs further study in order to improve its performance. Keywords: Polyphenols; anthocyanins; green tea; EGCG; olives; grapes</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf990767e</identifier><identifier>PMID: 10775372</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; Biosensing Techniques ; Chromatography, High Pressure Liquid - methods ; Diet ; Electrochemistry - methods ; Flavonoids ; Food industries ; Fruit - chemistry ; Fundamental and applied biological sciences. 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HPLC/DAD, DPV, and the biosensor approach were used as independent analytical techniques. Bare graphite screen-printed electrodes were employed in DPV and in the biosensor analysis. The most accurate data were obtained by HPLC/DAD analysis, while the DPV approach using screen-printed electrodes could represent a quick screening method for the determination of polyphenols in natural extracts. Use of the biosensor for the analysis of complex matrices needs further study in order to improve its performance. Keywords: Polyphenols; anthocyanins; green tea; EGCG; olives; grapes</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Diet</subject><subject>Electrochemistry - methods</subject><subject>Flavonoids</subject><subject>Food industries</subject><subject>Fruit - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Humans</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>Molecular Structure</subject><subject>Monophenol Monooxygenase</subject><subject>Phenols - analysis</subject><subject>Phenols - chemistry</subject><subject>Polymers - analysis</subject><subject>Polymers - chemistry</subject><subject>Polyphenols</subject><subject>Vegetables - chemistry</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EGL1DAUB_AgijuuHvwCkoMKwtZN2qZJj7Mz6qgDDrrqMWSSF83YJDVpwcEvb5cOqwdPj8f78efxR-gxJS8pKenlwbYt4Q2HO2hBWUkKRqm4ixZkOhaCNfQMPcj5QAgRjJP76IwSzlnFywX6vYq-V8nlGLDyMXzDa2ctJAiDUx3ejV0G_CV2g_IehnS8wEvfQ4o3i9P4ysUMIcd0gVUweLPbri7XyzVeBtUds8vYxoR3sTv23yHEDq9hgORdUIOL4SG6Z9WU_-g0z9Hn16-uV5ti--HN29VyW6iKi6GwjaGGc61qA6Jt9sy0SrQ10KqtQOhaN2JfaqAN35fGMEtFY1vDKs2Y1Vqb6hw9n3P7FH-OkAfpXdbQdSpAHLPklNS8YWKCL2aoU8w5gZV9cl6lo6RE3jQtb5ue7JNT6Lj3YP6Rc7UTeHoCKmvV2aSCdvmvq2pRk2pixcxcHuDX7VmlH7LhFWfyevdJtuL95mt59VG-m_yz2Sud5SGOaWo6_-e_P0IjouM</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>Romani, A</creator><creator>Minunni, M</creator><creator>Mulinacci, N</creator><creator>Pinelli, P</creator><creator>Vincieri, F. 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The analyses were applied to a series of both standards and natural extracts derived from grape, olives, and green tea. The pure compounds include phenolic acids, flavones, flavonols, catechins, tannins, and oleuropein. HPLC/DAD, DPV, and the biosensor approach were used as independent analytical techniques. Bare graphite screen-printed electrodes were employed in DPV and in the biosensor analysis. The most accurate data were obtained by HPLC/DAD analysis, while the DPV approach using screen-printed electrodes could represent a quick screening method for the determination of polyphenols in natural extracts. Use of the biosensor for the analysis of complex matrices needs further study in order to improve its performance. Keywords: Polyphenols; anthocyanins; green tea; EGCG; olives; grapes</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>10775372</pmid><doi>10.1021/jf990767e</doi><tpages>7</tpages></addata></record> |
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| subjects | Biological and medical sciences Biosensing Techniques Chromatography, High Pressure Liquid - methods Diet Electrochemistry - methods Flavonoids Food industries Fruit - chemistry Fundamental and applied biological sciences. Psychology General aspects Humans Methods of analysis, processing and quality control, regulation, standards Molecular Structure Monophenol Monooxygenase Phenols - analysis Phenols - chemistry Polymers - analysis Polymers - chemistry Polyphenols Vegetables - chemistry |
| title | Comparison among Differential Pulse Voltammetry, Amperometric Biosensor, and HPLC/DAD Analysis for Polyphenol Determination |
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