In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin secretion
To investigate the role of Transforming Growth Factor beta 1 on differentiation of human endometrial stromal cells. A prospective study. Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence an...
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| Veröffentlicht in: | Journal of the Pakistan Medical Association 2000-01, Vol.50 (1), p.17-19 |
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| container_title | Journal of the Pakistan Medical Association |
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| creator | Celikkanat, H Atac, B Piskin, K Helvacioglu, A Gokmen, O Cakar, N Beksac, M S |
| description | To investigate the role of Transforming Growth Factor beta 1 on differentiation of human endometrial stromal cells.
A prospective study.
Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence and absence of TGF b 1. The influence of TGF beta 1 were measured by prolactin production expressed as nanograms of prolactin/mg of total DNA in cells measured by diphenylamine reaction.
The levels of prolactin in the culture medium with and without TGF beta 1 were 0.16 +/- 0.27 ng/microgram DNA/day and 0.24 +/- 0.41 ng/microgram DNA/day respectively. There was no significant difference between groups.
There is no direct effect of TGF beta 1 on differentiation of human endometrial stromal cells. However it may involve in more complex relationship in this process. |
| format | Article |
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A prospective study.
Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence and absence of TGF b 1. The influence of TGF beta 1 were measured by prolactin production expressed as nanograms of prolactin/mg of total DNA in cells measured by diphenylamine reaction.
The levels of prolactin in the culture medium with and without TGF beta 1 were 0.16 +/- 0.27 ng/microgram DNA/day and 0.24 +/- 0.41 ng/microgram DNA/day respectively. There was no significant difference between groups.
There is no direct effect of TGF beta 1 on differentiation of human endometrial stromal cells. However it may involve in more complex relationship in this process.</description><identifier>ISSN: 0030-9982</identifier><identifier>PMID: 10770042</identifier><language>eng</language><publisher>Pakistan</publisher><subject>Adult ; Cell Differentiation - physiology ; Cells, Cultured ; Endometrium - cytology ; Female ; Humans ; Prolactin - secretion ; Prospective Studies ; Stromal Cells ; Transforming Growth Factor beta - physiology</subject><ispartof>Journal of the Pakistan Medical Association, 2000-01, Vol.50 (1), p.17-19</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10770042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Celikkanat, H</creatorcontrib><creatorcontrib>Atac, B</creatorcontrib><creatorcontrib>Piskin, K</creatorcontrib><creatorcontrib>Helvacioglu, A</creatorcontrib><creatorcontrib>Gokmen, O</creatorcontrib><creatorcontrib>Cakar, N</creatorcontrib><creatorcontrib>Beksac, M S</creatorcontrib><title>In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin secretion</title><title>Journal of the Pakistan Medical Association</title><addtitle>J Pak Med Assoc</addtitle><description>To investigate the role of Transforming Growth Factor beta 1 on differentiation of human endometrial stromal cells.
A prospective study.
Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence and absence of TGF b 1. The influence of TGF beta 1 were measured by prolactin production expressed as nanograms of prolactin/mg of total DNA in cells measured by diphenylamine reaction.
The levels of prolactin in the culture medium with and without TGF beta 1 were 0.16 +/- 0.27 ng/microgram DNA/day and 0.24 +/- 0.41 ng/microgram DNA/day respectively. There was no significant difference between groups.
There is no direct effect of TGF beta 1 on differentiation of human endometrial stromal cells. However it may involve in more complex relationship in this process.</description><subject>Adult</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Endometrium - cytology</subject><subject>Female</subject><subject>Humans</subject><subject>Prolactin - secretion</subject><subject>Prospective Studies</subject><subject>Stromal Cells</subject><subject>Transforming Growth Factor beta - physiology</subject><issn>0030-9982</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE9PAyEQxTlobK1-BcPJ2yaw7MKuN9P4p0kTL3resDC0mAUqsBpPfnVprKeXTH7z3rw5Q0tCGKn6vqsX6DKld0Jq3hJygRaUCEFIUy_Rz8bjT5tjwNoaAxF8tjLb4HEweD876TF4HRzkaOWEUyFdUQXTlO4wlBWVj2iO0icTorN-h3cxfOU9NlLlEPEIWWKKi-UhhqnMrMcJVIRjzBU6N3JKcH3SFXp7fHhdP1fbl6fN-n5bHWhd58qYtlNdq1rdyUZDp1TLBaG1Zt1IgREuG9YYkLxnrR6ZYlzREQxriOi5HDlbods_33LDxwwpD86mYwvpIcxpEJQ0gvaigDcncB4d6OEQrZPxe_h_GfsFCDppyQ</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Celikkanat, H</creator><creator>Atac, B</creator><creator>Piskin, K</creator><creator>Helvacioglu, A</creator><creator>Gokmen, O</creator><creator>Cakar, N</creator><creator>Beksac, M S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin secretion</title><author>Celikkanat, H ; Atac, B ; Piskin, K ; Helvacioglu, A ; Gokmen, O ; Cakar, N ; Beksac, M S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p122t-ff58c85c5d8a4de8cc567012d38b1e306a434fea6935db3c36c1bef340796ab63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Endometrium - cytology</topic><topic>Female</topic><topic>Humans</topic><topic>Prolactin - secretion</topic><topic>Prospective Studies</topic><topic>Stromal Cells</topic><topic>Transforming Growth Factor beta - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Celikkanat, H</creatorcontrib><creatorcontrib>Atac, B</creatorcontrib><creatorcontrib>Piskin, K</creatorcontrib><creatorcontrib>Helvacioglu, A</creatorcontrib><creatorcontrib>Gokmen, O</creatorcontrib><creatorcontrib>Cakar, N</creatorcontrib><creatorcontrib>Beksac, M S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the Pakistan Medical Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Celikkanat, H</au><au>Atac, B</au><au>Piskin, K</au><au>Helvacioglu, A</au><au>Gokmen, O</au><au>Cakar, N</au><au>Beksac, M S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin secretion</atitle><jtitle>Journal of the Pakistan Medical Association</jtitle><addtitle>J Pak Med Assoc</addtitle><date>2000-01</date><risdate>2000</risdate><volume>50</volume><issue>1</issue><spage>17</spage><epage>19</epage><pages>17-19</pages><issn>0030-9982</issn><abstract>To investigate the role of Transforming Growth Factor beta 1 on differentiation of human endometrial stromal cells.
A prospective study.
Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence and absence of TGF b 1. The influence of TGF beta 1 were measured by prolactin production expressed as nanograms of prolactin/mg of total DNA in cells measured by diphenylamine reaction.
The levels of prolactin in the culture medium with and without TGF beta 1 were 0.16 +/- 0.27 ng/microgram DNA/day and 0.24 +/- 0.41 ng/microgram DNA/day respectively. There was no significant difference between groups.
There is no direct effect of TGF beta 1 on differentiation of human endometrial stromal cells. However it may involve in more complex relationship in this process.</abstract><cop>Pakistan</cop><pmid>10770042</pmid><tpages>3</tpages></addata></record> |
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| language | eng |
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| subjects | Adult Cell Differentiation - physiology Cells, Cultured Endometrium - cytology Female Humans Prolactin - secretion Prospective Studies Stromal Cells Transforming Growth Factor beta - physiology |
| title | In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin secretion |
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