Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR
Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction...
Gespeichert in:
| Veröffentlicht in: | Journal of medical entomology 2001-07, Vol.38 (4), p.475-479 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 479 |
|---|---|
| container_issue | 4 |
| container_start_page | 475 |
| container_title | Journal of medical entomology |
| container_volume | 38 |
| creator | Kow, Chung Youne Koon, Lim Loo Yin, Pang Fung |
| description | Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN-4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%). |
| doi_str_mv | 10.1603/0022-2585-38.4.475 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_71045081</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A535152135</galeid><sourcerecordid>A535152135</sourcerecordid><originalsourceid>FETCH-LOGICAL-b409t-3de76b2a896f5f606b4a9f168f951ddb83ccab222f347ae50beb71b978d69aa3</originalsourceid><addsrcrecordid>eNqNkc-O0zAQhyMEYsvCC3BAvoDgkOI_ceJwq1IWkBaB2IqrNbbHxShNgp0c-go8Na5a2CvywdL4-43H_oriOaNrVlPxllLOSy6VLIVaV-uqkQ-KFWuFKnnL1cNi9Q-4Kp6k9JNSqljVPi6uGKuaWvB6Vfze4ox2DuNARk-2OOwXJN9DXBImEgZyE7B3pINl_2Mmn6FHskGXjwD3x2kOBAb3t9KbcQp2XhJ5vQ3TjBHekW7pgw0O8M2p2V0Y9jCNEYk5kt1xwvJuQht8sORr9-1p8chDn_DZZb8udjfvd93H8vbLh0_d5rY0FW3nUjhsasNBtbWXvqa1qaD1rFa-lcw5o4S1YDjnXlQNoKQGTcNM2yhXtwDiunh1bjvF8deCadaHkCz2PQw4Lkk3jFYyf1QG12dwn5-tw-DHOYLNy-Eh2HFAH3J9I4VkkjMhc4CfAzaOKUX0eorhAPGoGdUnY_okRJ-EaKF0pbOxHHpxGWcxB3T3kYuiDLy8AJAs9D7CYEO65yqqaFOrzNEzZ8KYh_ufu_8AR6usaA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71045081</pqid></control><display><type>article</type><title>Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR</title><source>MEDLINE</source><source>BioOne Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Kow, Chung Youne ; Koon, Lim Loo ; Yin, Pang Fung</creator><creatorcontrib>Kow, Chung Youne ; Koon, Lim Loo ; Yin, Pang Fung</creatorcontrib><description>Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN-4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%).</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1603/0022-2585-38.4.475</identifier><identifier>PMID: 11476326</identifier><identifier>CODEN: JMENA6</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Aedes - virology ; Aedes aegypti ; Aedes albopictus ; Animals ; Asian tiger mosquito ; Base Sequence ; Biological and medical sciences ; dengue ; Dengue virus ; Dengue Virus - genetics ; Dengue Virus - isolation & purification ; DNA, Viral - analysis ; Fundamental and applied biological sciences. Psychology ; Humans ; Male ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Molecular Sequence Data ; Mosquitoes ; polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Singapore ; Vectors. Intermediate hosts ; vertical transmission</subject><ispartof>Journal of medical entomology, 2001-07, Vol.38 (4), p.475-479</ispartof><rights>Entomological Society of America</rights><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2001 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b409t-3de76b2a896f5f606b4a9f168f951ddb83ccab222f347ae50beb71b978d69aa3</citedby><cites>FETCH-LOGICAL-b409t-3de76b2a896f5f606b4a9f168f951ddb83ccab222f347ae50beb71b978d69aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1603/0022-2585-38.4.475$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,780,784,26978,27924,27925,52363</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14080768$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11476326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kow, Chung Youne</creatorcontrib><creatorcontrib>Koon, Lim Loo</creatorcontrib><creatorcontrib>Yin, Pang Fung</creatorcontrib><title>Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN-4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%).</description><subject>Aedes - virology</subject><subject>Aedes aegypti</subject><subject>Aedes albopictus</subject><subject>Animals</subject><subject>Asian tiger mosquito</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>dengue</subject><subject>Dengue virus</subject><subject>Dengue Virus - genetics</subject><subject>Dengue Virus - isolation & purification</subject><subject>DNA, Viral - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Molecular Sequence Data</subject><subject>Mosquitoes</subject><subject>polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Singapore</subject><subject>Vectors. Intermediate hosts</subject><subject>vertical transmission</subject><issn>0022-2585</issn><issn>1938-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O0zAQhyMEYsvCC3BAvoDgkOI_ceJwq1IWkBaB2IqrNbbHxShNgp0c-go8Na5a2CvywdL4-43H_oriOaNrVlPxllLOSy6VLIVaV-uqkQ-KFWuFKnnL1cNi9Q-4Kp6k9JNSqljVPi6uGKuaWvB6Vfze4ox2DuNARk-2OOwXJN9DXBImEgZyE7B3pINl_2Mmn6FHskGXjwD3x2kOBAb3t9KbcQp2XhJ5vQ3TjBHekW7pgw0O8M2p2V0Y9jCNEYk5kt1xwvJuQht8sORr9-1p8chDn_DZZb8udjfvd93H8vbLh0_d5rY0FW3nUjhsasNBtbWXvqa1qaD1rFa-lcw5o4S1YDjnXlQNoKQGTcNM2yhXtwDiunh1bjvF8deCadaHkCz2PQw4Lkk3jFYyf1QG12dwn5-tw-DHOYLNy-Eh2HFAH3J9I4VkkjMhc4CfAzaOKUX0eorhAPGoGdUnY_okRJ-EaKF0pbOxHHpxGWcxB3T3kYuiDLy8AJAs9D7CYEO65yqqaFOrzNEzZ8KYh_ufu_8AR6usaA</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Kow, Chung Youne</creator><creator>Koon, Lim Loo</creator><creator>Yin, Pang Fung</creator><general>Entomological Society of America</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR</title><author>Kow, Chung Youne ; Koon, Lim Loo ; Yin, Pang Fung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b409t-3de76b2a896f5f606b4a9f168f951ddb83ccab222f347ae50beb71b978d69aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aedes - virology</topic><topic>Aedes aegypti</topic><topic>Aedes albopictus</topic><topic>Animals</topic><topic>Asian tiger mosquito</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>dengue</topic><topic>Dengue virus</topic><topic>Dengue Virus - genetics</topic><topic>Dengue Virus - isolation & purification</topic><topic>DNA, Viral - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>Molecular Sequence Data</topic><topic>Mosquitoes</topic><topic>polymerase chain reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Singapore</topic><topic>Vectors. Intermediate hosts</topic><topic>vertical transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kow, Chung Youne</creatorcontrib><creatorcontrib>Koon, Lim Loo</creatorcontrib><creatorcontrib>Yin, Pang Fung</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kow, Chung Youne</au><au>Koon, Lim Loo</au><au>Yin, Pang Fung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>38</volume><issue>4</issue><spage>475</spage><epage>479</epage><pages>475-479</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><coden>JMENA6</coden><abstract>Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN-4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%).</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>11476326</pmid><doi>10.1603/0022-2585-38.4.475</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 2001-07, Vol.38 (4), p.475-479 |
| issn | 0022-2585 1938-2928 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71045081 |
| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Aedes - virology Aedes aegypti Aedes albopictus Animals Asian tiger mosquito Base Sequence Biological and medical sciences dengue Dengue virus Dengue Virus - genetics Dengue Virus - isolation & purification DNA, Viral - analysis Fundamental and applied biological sciences. Psychology Humans Male Medically important nuisances and vectors, pests of stored products and materials: population survey and control Molecular Sequence Data Mosquitoes polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction Singapore Vectors. Intermediate hosts vertical transmission |
| title | Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T21%3A55%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20Dengue%20Viruses%20in%20Field%20Caught%20Male%20Aedes%20aegypti%20and%20Aedes%20albopictus%20(Diptera:%20Culicidae)%20in%20Singapore%20by%20Type-Specific%20PCR&rft.jtitle=Journal%20of%20medical%20entomology&rft.au=Kow,%20Chung%20Youne&rft.date=2001-07-01&rft.volume=38&rft.issue=4&rft.spage=475&rft.epage=479&rft.pages=475-479&rft.issn=0022-2585&rft.eissn=1938-2928&rft.coden=JMENA6&rft_id=info:doi/10.1603/0022-2585-38.4.475&rft_dat=%3Cgale_proqu%3EA535152135%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71045081&rft_id=info:pmid/11476326&rft_galeid=A535152135&rfr_iscdi=true |