An immunoblotting method for high-resolution isoelectric focusing of protein isoforms on immobilized pH gradients

Post‐translational modifications such as phosphorylation and acetylation are important elements for regulating the activity of enzymes or structural proteins. These modifications give rise to isoforms that are often not resolved by separation methods relying on the size of proteins. Here, we optimiz...

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Veröffentlicht in:	Electrophoresis 2001-06, Vol.22 (10), p.1887-1893
Hauptverfasser:	Towbin, Harry, Özbey, Özcan, Zingel, Otto
Format:	Artikel
Sprache:	eng
Schlagworte:	14-3-3 Proteins Electrophoresis, Gel, Two-Dimensional Humans Hydrogen-Ion Concentration Immunoblotting Immunoblotting - instrumentation Immunoblotting - methods Isoelectric focusing Isoelectric Focusing - instrumentation Isoelectric Focusing - methods Isoelectric Point Isoforms Microtubule Proteins Phosphoproteins - chemistry Phosphoproteins - isolation & purification Protein Isoforms - chemistry Protein Isoforms - isolation & purification Protein Processing, Post-Translational Proteins - chemistry Proteins - isolation & purification Stathmin Tubulin - chemistry Tubulin - isolation & purification Tyrosine 3-Monooxygenase - chemistry Tyrosine 3-Monooxygenase - isolation & purification
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