The effects of VEGF on survival of a random flap in the rat: examination of various routes of administration
The purpose of the present study was to determine the effects of vascular endothelial growth factor (VEGF) on survival of a full thickness random pattern, McFarlane musculocutaneous flap in the rat. In addition, this study examined a number of different methods of VEGF delivery in an attempt to dete...
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| Veröffentlicht in: | British journal of plastic surgery 2000-04, Vol.53 (3), p.234-239 |
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| creator | Kryger, Zol Zhang, Feng Lineaweaver, William C. Dogan, Teoman Cheng, Chester Buncke, Harry J. |
| description | The purpose of the present study was to determine the effects of vascular endothelial growth factor (VEGF) on survival of a full thickness random pattern, McFarlane musculocutaneous flap in the rat. In addition, this study examined a number of different methods of VEGF delivery in an attempt to determine the most effective route of administration. A 2×8 cm full thickness dorsal flap with the pedicle remaining attached at the anterior end was elevated in 72 male Sprague-Dawley rats. The rats were randomised into six groups and immediately received the following treatment: Group I (
n=12): systemic VEGF injection into the femoral vein (50 μg/ml); Group II (
n=10): multiple systemic VEGF injections at 0, 24 and 48 h post flap elevation (50 μg/ml); Group III (
n=12): subdermal VEGF injection into the flap (1 μg/ml); Group IV (
n=12): subfascial VEGF injections into the recipient bed (1 μg/ml); Group V (
n=10): topical VEGF onto the recipient bed (1 μg/ml); Group VI (
n=16): control group with no treatment. Following 5 days recovery, the area of flap survival was measured. Mean flap survival ranged from 91% in Group II to 78% in Group V, and was significantly greater in all experimental groups (
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| doi_str_mv | 10.1054/bjps.1999.3315 |
| format | Article |
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n=12): systemic VEGF injection into the femoral vein (50 μg/ml); Group II (
n=10): multiple systemic VEGF injections at 0, 24 and 48 h post flap elevation (50 μg/ml); Group III (
n=12): subdermal VEGF injection into the flap (1 μg/ml); Group IV (
n=12): subfascial VEGF injections into the recipient bed (1 μg/ml); Group V (
n=10): topical VEGF onto the recipient bed (1 μg/ml); Group VI (
n=16): control group with no treatment. Following 5 days recovery, the area of flap survival was measured. Mean flap survival ranged from 91% in Group II to 78% in Group V, and was significantly greater in all experimental groups (
P<0.001 for Groups I–IV and
P<0.05 for Group V) as compared to the control group (mean survival of 66%). The only significant difference between the experimental groups was between the mean survival in Group II and Group V (
P<0.05). Histological analysis demonstrated a qualitatively greater amount of granulation tissue and neovascularisation in the experimental groups. These results support the notion that VEGF rescues tissue at risk of hypoxic damage by inducing angiogenesis, and the use of growth factors such as VEGF holds promise as a method of increasing skin viability.</description><identifier>ISSN: 0007-1226</identifier><identifier>EISSN: 1465-3087</identifier><identifier>DOI: 10.1054/bjps.1999.3315</identifier><identifier>PMID: 10738331</identifier><identifier>CODEN: BJPSAZ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Administration, Topical ; Animals ; Biological and medical sciences ; Drug Administration Schedule ; Endothelial Growth Factors - administration & dosage ; experimental rat model ; Graft Survival - drug effects ; Injections, Intravenous ; Injections, Subcutaneous ; Lymphokines - administration & dosage ; Male ; Medical sciences ; Neovascularization, Physiologic ; random pattern flap survival ; Rats ; Rats, Sprague-Dawley ; route of administration ; Skin plastic surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical Flaps - pathology ; Surgical Flaps - physiology ; Tissue Survival - physiology ; vascular endothelial growth factor ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors</subject><ispartof>British journal of plastic surgery, 2000-04, Vol.53 (3), p.234-239</ispartof><rights>2000 The British Association of Plastic Surgeons</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 The British Association of Plastic Surgeons.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-f4e320a0c5bd5207d64c3309d2bb21e006d8327ab94e6a028d264773fb59aa793</citedby><cites>FETCH-LOGICAL-c475t-f4e320a0c5bd5207d64c3309d2bb21e006d8327ab94e6a028d264773fb59aa793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1350859$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10738331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kryger, Zol</creatorcontrib><creatorcontrib>Zhang, Feng</creatorcontrib><creatorcontrib>Lineaweaver, William C.</creatorcontrib><creatorcontrib>Dogan, Teoman</creatorcontrib><creatorcontrib>Cheng, Chester</creatorcontrib><creatorcontrib>Buncke, Harry J.</creatorcontrib><title>The effects of VEGF on survival of a random flap in the rat: examination of various routes of administration</title><title>British journal of plastic surgery</title><addtitle>Br J Plast Surg</addtitle><description>The purpose of the present study was to determine the effects of vascular endothelial growth factor (VEGF) on survival of a full thickness random pattern, McFarlane musculocutaneous flap in the rat. In addition, this study examined a number of different methods of VEGF delivery in an attempt to determine the most effective route of administration. A 2×8 cm full thickness dorsal flap with the pedicle remaining attached at the anterior end was elevated in 72 male Sprague-Dawley rats. The rats were randomised into six groups and immediately received the following treatment: Group I (
n=12): systemic VEGF injection into the femoral vein (50 μg/ml); Group II (
n=10): multiple systemic VEGF injections at 0, 24 and 48 h post flap elevation (50 μg/ml); Group III (
n=12): subdermal VEGF injection into the flap (1 μg/ml); Group IV (
n=12): subfascial VEGF injections into the recipient bed (1 μg/ml); Group V (
n=10): topical VEGF onto the recipient bed (1 μg/ml); Group VI (
n=16): control group with no treatment. Following 5 days recovery, the area of flap survival was measured. Mean flap survival ranged from 91% in Group II to 78% in Group V, and was significantly greater in all experimental groups (
P<0.001 for Groups I–IV and
P<0.05 for Group V) as compared to the control group (mean survival of 66%). The only significant difference between the experimental groups was between the mean survival in Group II and Group V (
P<0.05). Histological analysis demonstrated a qualitatively greater amount of granulation tissue and neovascularisation in the experimental groups. These results support the notion that VEGF rescues tissue at risk of hypoxic damage by inducing angiogenesis, and the use of growth factors such as VEGF holds promise as a method of increasing skin viability.</description><subject>Administration, Topical</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Drug Administration Schedule</subject><subject>Endothelial Growth Factors - administration & dosage</subject><subject>experimental rat model</subject><subject>Graft Survival - drug effects</subject><subject>Injections, Intravenous</subject><subject>Injections, Subcutaneous</subject><subject>Lymphokines - administration & dosage</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neovascularization, Physiologic</subject><subject>random pattern flap survival</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>route of administration</subject><subject>Skin plastic surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical Flaps - pathology</subject><subject>Surgical Flaps - physiology</subject><subject>Tissue Survival - physiology</subject><subject>vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><issn>0007-1226</issn><issn>1465-3087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kTtrHDEURkVwiDdO2pRGhUk3Gz1HM-mM8ToBQxonrbgjXRGZeaylmSX-99F4F-ImlZDu-S4fR4R84mzLmVZfusd93vK2bbdScv2GbLiqdSVZY87IhjFmKi5EfU7e5_xYrq0S8h0558zIpgQ2pH_4jRRDQDdnOgX66_ZuR6eR5iUd4gH69Q1ogtFPAw097Gkc6VwyCeavFP_AEEeYY0kU8AApTkumaVpmfFkHvsxjntML84G8DdBn_Hg6L8jP3e3Dzbfq_sfd95vr-8opo-cqKJSCAXO681ow42vlpGStF10nODJW-0YKA12rsAYmGi9qZYwMnW4BTCsvyOfj3n2anhbMsx1idtj3MGLpZw1nspZcFXB7BF2ack4Y7D7FAdKz5cyufu3q165-7eq3BC5Pm5duQP8KPwotwNUJgOygD8Wci_kfJzVr9NqwOWJYNBwiJptdxNGhj6l8hfVT_F-Fv_YUllY</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>Kryger, Zol</creator><creator>Zhang, Feng</creator><creator>Lineaweaver, William C.</creator><creator>Dogan, Teoman</creator><creator>Cheng, Chester</creator><creator>Buncke, Harry J.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000401</creationdate><title>The effects of VEGF on survival of a random flap in the rat: examination of various routes of administration</title><author>Kryger, Zol ; Zhang, Feng ; Lineaweaver, William C. ; Dogan, Teoman ; Cheng, Chester ; Buncke, Harry J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-f4e320a0c5bd5207d64c3309d2bb21e006d8327ab94e6a028d264773fb59aa793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Administration, Topical</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Drug Administration Schedule</topic><topic>Endothelial Growth Factors - administration & dosage</topic><topic>experimental rat model</topic><topic>Graft Survival - drug effects</topic><topic>Injections, Intravenous</topic><topic>Injections, Subcutaneous</topic><topic>Lymphokines - administration & dosage</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neovascularization, Physiologic</topic><topic>random pattern flap survival</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>route of administration</topic><topic>Skin plastic surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical Flaps - pathology</topic><topic>Surgical Flaps - physiology</topic><topic>Tissue Survival - physiology</topic><topic>vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kryger, Zol</creatorcontrib><creatorcontrib>Zhang, Feng</creatorcontrib><creatorcontrib>Lineaweaver, William C.</creatorcontrib><creatorcontrib>Dogan, Teoman</creatorcontrib><creatorcontrib>Cheng, Chester</creatorcontrib><creatorcontrib>Buncke, Harry J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of plastic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kryger, Zol</au><au>Zhang, Feng</au><au>Lineaweaver, William C.</au><au>Dogan, Teoman</au><au>Cheng, Chester</au><au>Buncke, Harry J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of VEGF on survival of a random flap in the rat: examination of various routes of administration</atitle><jtitle>British journal of plastic surgery</jtitle><addtitle>Br J Plast Surg</addtitle><date>2000-04-01</date><risdate>2000</risdate><volume>53</volume><issue>3</issue><spage>234</spage><epage>239</epage><pages>234-239</pages><issn>0007-1226</issn><eissn>1465-3087</eissn><coden>BJPSAZ</coden><abstract>The purpose of the present study was to determine the effects of vascular endothelial growth factor (VEGF) on survival of a full thickness random pattern, McFarlane musculocutaneous flap in the rat. In addition, this study examined a number of different methods of VEGF delivery in an attempt to determine the most effective route of administration. A 2×8 cm full thickness dorsal flap with the pedicle remaining attached at the anterior end was elevated in 72 male Sprague-Dawley rats. The rats were randomised into six groups and immediately received the following treatment: Group I (
n=12): systemic VEGF injection into the femoral vein (50 μg/ml); Group II (
n=10): multiple systemic VEGF injections at 0, 24 and 48 h post flap elevation (50 μg/ml); Group III (
n=12): subdermal VEGF injection into the flap (1 μg/ml); Group IV (
n=12): subfascial VEGF injections into the recipient bed (1 μg/ml); Group V (
n=10): topical VEGF onto the recipient bed (1 μg/ml); Group VI (
n=16): control group with no treatment. Following 5 days recovery, the area of flap survival was measured. Mean flap survival ranged from 91% in Group II to 78% in Group V, and was significantly greater in all experimental groups (
P<0.001 for Groups I–IV and
P<0.05 for Group V) as compared to the control group (mean survival of 66%). The only significant difference between the experimental groups was between the mean survival in Group II and Group V (
P<0.05). Histological analysis demonstrated a qualitatively greater amount of granulation tissue and neovascularisation in the experimental groups. These results support the notion that VEGF rescues tissue at risk of hypoxic damage by inducing angiogenesis, and the use of growth factors such as VEGF holds promise as a method of increasing skin viability.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10738331</pmid><doi>10.1054/bjps.1999.3315</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1226 |
| ispartof | British journal of plastic surgery, 2000-04, Vol.53 (3), p.234-239 |
| issn | 0007-1226 1465-3087 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Administration, Topical Animals Biological and medical sciences Drug Administration Schedule Endothelial Growth Factors - administration & dosage experimental rat model Graft Survival - drug effects Injections, Intravenous Injections, Subcutaneous Lymphokines - administration & dosage Male Medical sciences Neovascularization, Physiologic random pattern flap survival Rats Rats, Sprague-Dawley route of administration Skin plastic surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgical Flaps - pathology Surgical Flaps - physiology Tissue Survival - physiology vascular endothelial growth factor Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| title | The effects of VEGF on survival of a random flap in the rat: examination of various routes of administration |
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