Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children
When inserted into a human incision wound, the Cellstick® device harvests inflammatory cells and collects wound fluid, reflecting time‐related changes in cell populations and in wound fluid composition. Hyaluronic acid has been postulated to be an important factor in scar reduction in wound healing...
Gespeichert in:
| Veröffentlicht in: | Wound repair and regeneration 2001-05, Vol.9 (3), p.200-204 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 204 |
|---|---|
| container_issue | 3 |
| container_start_page | 200 |
| container_title | Wound repair and regeneration |
| container_volume | 9 |
| creator | Pajulo, Olli T. Pulkki, Kari J. Lertola, Kaarlo K. Alanen, Markku S. Reunanen, Mikko S. Virtanen, Riitta V. Mattila-Vuori, Armi I. Viljanto, Jouko A. |
| description | When inserted into a human incision wound, the Cellstick® device harvests inflammatory cells and collects wound fluid, reflecting time‐related changes in cell populations and in wound fluid composition. Hyaluronic acid has been postulated to be an important factor in scar reduction in wound healing and in scarless fetal wound healing. The aim of this work was to determine the concentration and variation of hyaluronic acid and proportions of wound cells in closed surgical wounds in children at two time points. The Cellstick® device was inserted subcutaneously into the wound at the end of an elective inguinal hernia operation on 37 healthy boys, and the devices were removed 3±1 or 24±3 hours after surgery. Haluronic acid concentration was measured from the wound fluid and a differential count of the wound cells was performed. There was a significant decrease in hyaluronic acid concentration from 3±1 to 24±3 hours after surgery (p |
| doi_str_mv | 10.1046/j.1524-475x.2001.00200.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71035926</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71035926</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4020-25754d81208c4e32d672c2d1243200f2c1624693763a9873cbd5b791576828a93</originalsourceid><addsrcrecordid>eNqNkN1OGzEQhS1Exf8rIF_1bhf_24u4QVEbkCiVUFG4sxzbURycXVjvNslL9SF4MrwkpbeVRpqRfM7xzAcAxKjEiImLRYk5YQWTfF0ShHCJUG7leg8c_X142s8zErLAFZGH4DilBUKI80odgEOMmSQCiyNgbjYm9m1TBwuNDQ6GOpcNKTQ1XDV97eAs9sFdwmtoY8gyE2HqereBq9DNYTf3cORjTF2wz29_oPO_g_VDip2H6Fpfn4IvMxOTP9v1E_D4_duv0U1x93N8O7q-KyzLyxeES86cwgQpyzwlTkhiicOE0XzajFgsCBMVlYKaSklqp45PZYW5FIooU9ET8HWb-9I2r71PnV6GZPNqpvZNn7TEiPKKiCxUW6Ftm5RaP9MvbViadqMx0gNevdADRT3g1QNe_YFXr7P1fPdHP11698-445kFV1vBKkS_-e9gPXl4yEO2F1t7SJ1ff9pN-6yFpJLryf1YozG9VxOk9A_6Dtd3l14</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71035926</pqid></control><display><type>article</type><title>Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Pajulo, Olli T. ; Pulkki, Kari J. ; Lertola, Kaarlo K. ; Alanen, Markku S. ; Reunanen, Mikko S. ; Virtanen, Riitta V. ; Mattila-Vuori, Armi I. ; Viljanto, Jouko A.</creator><creatorcontrib>Pajulo, Olli T. ; Pulkki, Kari J. ; Lertola, Kaarlo K. ; Alanen, Markku S. ; Reunanen, Mikko S. ; Virtanen, Riitta V. ; Mattila-Vuori, Armi I. ; Viljanto, Jouko A.</creatorcontrib><description>When inserted into a human incision wound, the Cellstick® device harvests inflammatory cells and collects wound fluid, reflecting time‐related changes in cell populations and in wound fluid composition. Hyaluronic acid has been postulated to be an important factor in scar reduction in wound healing and in scarless fetal wound healing. The aim of this work was to determine the concentration and variation of hyaluronic acid and proportions of wound cells in closed surgical wounds in children at two time points. The Cellstick® device was inserted subcutaneously into the wound at the end of an elective inguinal hernia operation on 37 healthy boys, and the devices were removed 3±1 or 24±3 hours after surgery. Haluronic acid concentration was measured from the wound fluid and a differential count of the wound cells was performed. There was a significant decrease in hyaluronic acid concentration from 3±1 to 24±3 hours after surgery (p<0.001, Kruskal‐Wallis anova). The variance of hyaluronic acid concentration in wound fluid differed between the wounds at the two time points (p<0.01, Levene test for homogeneity of variance). A positive correlation between hyaluronic acid concentration and patient age (r=0.91, p<0.05, Spearman) at 3±1 hours post surgery and between HA and wound lymphocytes (r=0.38, p<0.05, Spearman) was also found. We conclude that the hyaluronic acid concentration in wound fluid peaks early in children and decreases significantly by 3 to 24 hours after surgery, and the concentrations in the wound fluid of healthy boys are more variable 3 hours than at 24 hours after surgery.</description><identifier>ISSN: 1067-1927</identifier><identifier>EISSN: 1524-475X</identifier><identifier>DOI: 10.1046/j.1524-475x.2001.00200.x</identifier><identifier>PMID: 11472616</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Inc</publisher><subject>Analysis of Variance ; Cell Count ; Child ; Child, Preschool ; Exudates and Transudates - chemistry ; Exudates and Transudates - immunology ; Hernia, Inguinal - surgery ; Humans ; Hyaluronic Acid - analysis ; Hyaluronic Acid - physiology ; Infant ; Leukocyte Count ; Lymphocyte Count ; Male ; Neutrophils ; Specimen Handling - instrumentation ; Statistics, Nonparametric ; Time Factors ; Wound Healing ; Wounds and Injuries - etiology ; Wounds and Injuries - immunology ; Wounds and Injuries - pathology</subject><ispartof>Wound repair and regeneration, 2001-05, Vol.9 (3), p.200-204</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4020-25754d81208c4e32d672c2d1243200f2c1624693763a9873cbd5b791576828a93</citedby><cites>FETCH-LOGICAL-c4020-25754d81208c4e32d672c2d1243200f2c1624693763a9873cbd5b791576828a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1524-475x.2001.00200.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1524-475x.2001.00200.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11472616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pajulo, Olli T.</creatorcontrib><creatorcontrib>Pulkki, Kari J.</creatorcontrib><creatorcontrib>Lertola, Kaarlo K.</creatorcontrib><creatorcontrib>Alanen, Markku S.</creatorcontrib><creatorcontrib>Reunanen, Mikko S.</creatorcontrib><creatorcontrib>Virtanen, Riitta V.</creatorcontrib><creatorcontrib>Mattila-Vuori, Armi I.</creatorcontrib><creatorcontrib>Viljanto, Jouko A.</creatorcontrib><title>Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children</title><title>Wound repair and regeneration</title><addtitle>Wound Repair Regen</addtitle><description>When inserted into a human incision wound, the Cellstick® device harvests inflammatory cells and collects wound fluid, reflecting time‐related changes in cell populations and in wound fluid composition. Hyaluronic acid has been postulated to be an important factor in scar reduction in wound healing and in scarless fetal wound healing. The aim of this work was to determine the concentration and variation of hyaluronic acid and proportions of wound cells in closed surgical wounds in children at two time points. The Cellstick® device was inserted subcutaneously into the wound at the end of an elective inguinal hernia operation on 37 healthy boys, and the devices were removed 3±1 or 24±3 hours after surgery. Haluronic acid concentration was measured from the wound fluid and a differential count of the wound cells was performed. There was a significant decrease in hyaluronic acid concentration from 3±1 to 24±3 hours after surgery (p<0.001, Kruskal‐Wallis anova). The variance of hyaluronic acid concentration in wound fluid differed between the wounds at the two time points (p<0.01, Levene test for homogeneity of variance). A positive correlation between hyaluronic acid concentration and patient age (r=0.91, p<0.05, Spearman) at 3±1 hours post surgery and between HA and wound lymphocytes (r=0.38, p<0.05, Spearman) was also found. We conclude that the hyaluronic acid concentration in wound fluid peaks early in children and decreases significantly by 3 to 24 hours after surgery, and the concentrations in the wound fluid of healthy boys are more variable 3 hours than at 24 hours after surgery.</description><subject>Analysis of Variance</subject><subject>Cell Count</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Exudates and Transudates - chemistry</subject><subject>Exudates and Transudates - immunology</subject><subject>Hernia, Inguinal - surgery</subject><subject>Humans</subject><subject>Hyaluronic Acid - analysis</subject><subject>Hyaluronic Acid - physiology</subject><subject>Infant</subject><subject>Leukocyte Count</subject><subject>Lymphocyte Count</subject><subject>Male</subject><subject>Neutrophils</subject><subject>Specimen Handling - instrumentation</subject><subject>Statistics, Nonparametric</subject><subject>Time Factors</subject><subject>Wound Healing</subject><subject>Wounds and Injuries - etiology</subject><subject>Wounds and Injuries - immunology</subject><subject>Wounds and Injuries - pathology</subject><issn>1067-1927</issn><issn>1524-475X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkN1OGzEQhS1Exf8rIF_1bhf_24u4QVEbkCiVUFG4sxzbURycXVjvNslL9SF4MrwkpbeVRpqRfM7xzAcAxKjEiImLRYk5YQWTfF0ShHCJUG7leg8c_X142s8zErLAFZGH4DilBUKI80odgEOMmSQCiyNgbjYm9m1TBwuNDQ6GOpcNKTQ1XDV97eAs9sFdwmtoY8gyE2HqereBq9DNYTf3cORjTF2wz29_oPO_g_VDip2H6Fpfn4IvMxOTP9v1E_D4_duv0U1x93N8O7q-KyzLyxeES86cwgQpyzwlTkhiicOE0XzajFgsCBMVlYKaSklqp45PZYW5FIooU9ET8HWb-9I2r71PnV6GZPNqpvZNn7TEiPKKiCxUW6Ftm5RaP9MvbViadqMx0gNevdADRT3g1QNe_YFXr7P1fPdHP11698-445kFV1vBKkS_-e9gPXl4yEO2F1t7SJ1ff9pN-6yFpJLryf1YozG9VxOk9A_6Dtd3l14</recordid><startdate>200105</startdate><enddate>200105</enddate><creator>Pajulo, Olli T.</creator><creator>Pulkki, Kari J.</creator><creator>Lertola, Kaarlo K.</creator><creator>Alanen, Markku S.</creator><creator>Reunanen, Mikko S.</creator><creator>Virtanen, Riitta V.</creator><creator>Mattila-Vuori, Armi I.</creator><creator>Viljanto, Jouko A.</creator><general>Blackwell Science Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200105</creationdate><title>Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children</title><author>Pajulo, Olli T. ; Pulkki, Kari J. ; Lertola, Kaarlo K. ; Alanen, Markku S. ; Reunanen, Mikko S. ; Virtanen, Riitta V. ; Mattila-Vuori, Armi I. ; Viljanto, Jouko A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4020-25754d81208c4e32d672c2d1243200f2c1624693763a9873cbd5b791576828a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Analysis of Variance</topic><topic>Cell Count</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Exudates and Transudates - chemistry</topic><topic>Exudates and Transudates - immunology</topic><topic>Hernia, Inguinal - surgery</topic><topic>Humans</topic><topic>Hyaluronic Acid - analysis</topic><topic>Hyaluronic Acid - physiology</topic><topic>Infant</topic><topic>Leukocyte Count</topic><topic>Lymphocyte Count</topic><topic>Male</topic><topic>Neutrophils</topic><topic>Specimen Handling - instrumentation</topic><topic>Statistics, Nonparametric</topic><topic>Time Factors</topic><topic>Wound Healing</topic><topic>Wounds and Injuries - etiology</topic><topic>Wounds and Injuries - immunology</topic><topic>Wounds and Injuries - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pajulo, Olli T.</creatorcontrib><creatorcontrib>Pulkki, Kari J.</creatorcontrib><creatorcontrib>Lertola, Kaarlo K.</creatorcontrib><creatorcontrib>Alanen, Markku S.</creatorcontrib><creatorcontrib>Reunanen, Mikko S.</creatorcontrib><creatorcontrib>Virtanen, Riitta V.</creatorcontrib><creatorcontrib>Mattila-Vuori, Armi I.</creatorcontrib><creatorcontrib>Viljanto, Jouko A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Wound repair and regeneration</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pajulo, Olli T.</au><au>Pulkki, Kari J.</au><au>Lertola, Kaarlo K.</au><au>Alanen, Markku S.</au><au>Reunanen, Mikko S.</au><au>Virtanen, Riitta V.</au><au>Mattila-Vuori, Armi I.</au><au>Viljanto, Jouko A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children</atitle><jtitle>Wound repair and regeneration</jtitle><addtitle>Wound Repair Regen</addtitle><date>2001-05</date><risdate>2001</risdate><volume>9</volume><issue>3</issue><spage>200</spage><epage>204</epage><pages>200-204</pages><issn>1067-1927</issn><eissn>1524-475X</eissn><abstract>When inserted into a human incision wound, the Cellstick® device harvests inflammatory cells and collects wound fluid, reflecting time‐related changes in cell populations and in wound fluid composition. Hyaluronic acid has been postulated to be an important factor in scar reduction in wound healing and in scarless fetal wound healing. The aim of this work was to determine the concentration and variation of hyaluronic acid and proportions of wound cells in closed surgical wounds in children at two time points. The Cellstick® device was inserted subcutaneously into the wound at the end of an elective inguinal hernia operation on 37 healthy boys, and the devices were removed 3±1 or 24±3 hours after surgery. Haluronic acid concentration was measured from the wound fluid and a differential count of the wound cells was performed. There was a significant decrease in hyaluronic acid concentration from 3±1 to 24±3 hours after surgery (p<0.001, Kruskal‐Wallis anova). The variance of hyaluronic acid concentration in wound fluid differed between the wounds at the two time points (p<0.01, Levene test for homogeneity of variance). A positive correlation between hyaluronic acid concentration and patient age (r=0.91, p<0.05, Spearman) at 3±1 hours post surgery and between HA and wound lymphocytes (r=0.38, p<0.05, Spearman) was also found. We conclude that the hyaluronic acid concentration in wound fluid peaks early in children and decreases significantly by 3 to 24 hours after surgery, and the concentrations in the wound fluid of healthy boys are more variable 3 hours than at 24 hours after surgery.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Inc</pub><pmid>11472616</pmid><doi>10.1046/j.1524-475x.2001.00200.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1067-1927 |
| ispartof | Wound repair and regeneration, 2001-05, Vol.9 (3), p.200-204 |
| issn | 1067-1927 1524-475X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71035926 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Analysis of Variance Cell Count Child Child, Preschool Exudates and Transudates - chemistry Exudates and Transudates - immunology Hernia, Inguinal - surgery Humans Hyaluronic Acid - analysis Hyaluronic Acid - physiology Infant Leukocyte Count Lymphocyte Count Male Neutrophils Specimen Handling - instrumentation Statistics, Nonparametric Time Factors Wound Healing Wounds and Injuries - etiology Wounds and Injuries - immunology Wounds and Injuries - pathology |
| title | Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T18%3A48%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hyaluronic%20acid%20in%20incision%20wound%20fluid:%20A%20clinical%20study%20with%20the%20Cellstick%C2%AE%20device%20in%20children&rft.jtitle=Wound%20repair%20and%20regeneration&rft.au=Pajulo,%20Olli%20T.&rft.date=2001-05&rft.volume=9&rft.issue=3&rft.spage=200&rft.epage=204&rft.pages=200-204&rft.issn=1067-1927&rft.eissn=1524-475X&rft_id=info:doi/10.1046/j.1524-475x.2001.00200.x&rft_dat=%3Cproquest_cross%3E71035926%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71035926&rft_id=info:pmid/11472616&rfr_iscdi=true |