Preparation and Characterization of Collagen-Elastin Matrices From Blood Vessels Intended as Small Diameter Vascular Grafts

: This study describes the selective removal of cell and cell residues from small and large diameter blood vessels for the preparation of tubular collagen:elastin matrices intended for small diameter vascular prosthesis. The results showed that total or partially devitalized collagen:elastin matrice...

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Veröffentlicht in:Artificial organs 2000-03, Vol.24 (3), p.217-223
Hauptverfasser: Goissis, Gilberto, Suzigan, Sueli, Parreira, Diderot Rodrigues, Maniglia, José Vitor, Braile, Domingo Marcolino, Raymundo, Selma
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container_end_page 223
container_issue 3
container_start_page 217
container_title Artificial organs
container_volume 24
creator Goissis, Gilberto
Suzigan, Sueli
Parreira, Diderot Rodrigues
Maniglia, José Vitor
Braile, Domingo Marcolino
Raymundo, Selma
description : This study describes the selective removal of cell and cell residues from small and large diameter blood vessels for the preparation of tubular collagen:elastin matrices intended for small diameter vascular prosthesis. The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. The efficiency of cell removal and the extent collagen and elastic fiber preservation were dependent on the segment and the type of blood vessel under study, with arteries characterized by a higher susceptibility of cell removal and better preservation of the collagen‐elastin matrix.
doi_str_mv 10.1046/j.1525-1594.2000.06537.x
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The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. 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The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. 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The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. The efficiency of cell removal and the extent collagen and elastic fiber preservation were dependent on the segment and the type of blood vessel under study, with arteries characterized by a higher susceptibility of cell removal and better preservation of the collagen‐elastin matrix.</abstract><cop>Boston, MA, USA</cop><pub>Blackwell Science Inc</pub><pmid>10759645</pmid><doi>10.1046/j.1525-1594.2000.06537.x</doi><tpages>7</tpages></addata></record>
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source MEDLINE; Access via Wiley Online Library
subjects Acellular
Alkalies
Animals
Aorta, Abdominal
Biocompatible Materials
Bioprosthesis
Blood Vessel Prosthesis
Blood Vessels - cytology
Calorimetry, Differential Scanning
Characterization
Collagen
Coloring Agents
Cross-Linking Reagents
Dimethyl Sulfoxide
Dogs
Elastin
Electrophysiology
Femoral Artery
Femoral Vein
Iliac Artery
Iliac Vein
Matrix
Microscopy, Electron, Scanning
Prosthesis Design
Solvents
Thoracic Arteries
Tissue Preservation - methods
Vena Cava, Inferior
Vena Cava, Superior
Vessels
title Preparation and Characterization of Collagen-Elastin Matrices From Blood Vessels Intended as Small Diameter Vascular Grafts
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