Preparation and Characterization of Collagen-Elastin Matrices From Blood Vessels Intended as Small Diameter Vascular Grafts
: This study describes the selective removal of cell and cell residues from small and large diameter blood vessels for the preparation of tubular collagen:elastin matrices intended for small diameter vascular prosthesis. The results showed that total or partially devitalized collagen:elastin matrice...
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Veröffentlicht in: | Artificial organs 2000-03, Vol.24 (3), p.217-223 |
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creator | Goissis, Gilberto Suzigan, Sueli Parreira, Diderot Rodrigues Maniglia, José Vitor Braile, Domingo Marcolino Raymundo, Selma |
description | : This study describes the selective removal of cell and cell residues from small and large diameter blood vessels for the preparation of tubular collagen:elastin matrices intended for small diameter vascular prosthesis. The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. The efficiency of cell removal and the extent collagen and elastic fiber preservation were dependent on the segment and the type of blood vessel under study, with arteries characterized by a higher susceptibility of cell removal and better preservation of the collagen‐elastin matrix. |
doi_str_mv | 10.1046/j.1525-1594.2000.06537.x |
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The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. 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The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. The efficiency of cell removal and the extent collagen and elastic fiber preservation were dependent on the segment and the type of blood vessel under study, with arteries characterized by a higher susceptibility of cell removal and better preservation of the collagen‐elastin matrix.</description><subject>Acellular</subject><subject>Alkalies</subject><subject>Animals</subject><subject>Aorta, Abdominal</subject><subject>Biocompatible Materials</subject><subject>Bioprosthesis</subject><subject>Blood Vessel Prosthesis</subject><subject>Blood Vessels - cytology</subject><subject>Calorimetry, Differential Scanning</subject><subject>Characterization</subject><subject>Collagen</subject><subject>Coloring Agents</subject><subject>Cross-Linking Reagents</subject><subject>Dimethyl Sulfoxide</subject><subject>Dogs</subject><subject>Elastin</subject><subject>Electrophysiology</subject><subject>Femoral Artery</subject><subject>Femoral Vein</subject><subject>Iliac Artery</subject><subject>Iliac Vein</subject><subject>Matrix</subject><subject>Microscopy, Electron, Scanning</subject><subject>Prosthesis Design</subject><subject>Solvents</subject><subject>Thoracic Arteries</subject><subject>Tissue Preservation - methods</subject><subject>Vena Cava, Inferior</subject><subject>Vena Cava, Superior</subject><subject>Vessels</subject><issn>0160-564X</issn><issn>1525-1594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAYRS0EokPhFZBX7BLs-C9ZsGin7VA0UFRKYWd9sR3I4MSDnRFTJCQk3pQnISFVxZKV_869n3wQwpTklHD5fJNTUYiMiornBSEkJ1Iwle_vocXdw320IFSSTEj-8QA9SmkzgooT-RAdUKJEJblYoB9vo9tChKENPYbe4uXn8WQGF9vv82Vo8DJ4D59cn516SEPb49cwxNa4hM9i6PCxD8Hia5eS8wmf94PrrbMYEn7Xgff4pIXOjY34GpLZeYi_f_5aRWiG9Bg9aMAn9-R2PUTvz06vli-z9cXqfHm0zgyXlcqKRihaUbCUC9lYCsxYVdalIkBoSZmta0l4UYE1FVTOVHXBnSyY4YoSEJIdomdz7zaGrzuXBt21ybjxV70Lu6RHjPGSsREsZ9DEkFJ0jd7GtoN4oynRk3q90ZNhPRnWk3r9V73ej9GntzN2defsP8HZ9Qi8mIFvrXc3_12sjy4up92Yz-Z8mwa3v8tD_KKlYkroD29WWl6RNVOvjvUl-wO3S6OE</recordid><startdate>200003</startdate><enddate>200003</enddate><creator>Goissis, Gilberto</creator><creator>Suzigan, Sueli</creator><creator>Parreira, Diderot Rodrigues</creator><creator>Maniglia, José Vitor</creator><creator>Braile, Domingo Marcolino</creator><creator>Raymundo, Selma</creator><general>Blackwell Science Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200003</creationdate><title>Preparation and Characterization of Collagen-Elastin Matrices From Blood Vessels Intended as Small Diameter Vascular Grafts</title><author>Goissis, Gilberto ; Suzigan, Sueli ; Parreira, Diderot Rodrigues ; Maniglia, José Vitor ; Braile, Domingo Marcolino ; Raymundo, Selma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4697-2f57191ad1456fd1a3cd78b870a01813dbb60429adc9a9ec9b24e623c4710a563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acellular</topic><topic>Alkalies</topic><topic>Animals</topic><topic>Aorta, Abdominal</topic><topic>Biocompatible Materials</topic><topic>Bioprosthesis</topic><topic>Blood Vessel Prosthesis</topic><topic>Blood Vessels - cytology</topic><topic>Calorimetry, Differential Scanning</topic><topic>Characterization</topic><topic>Collagen</topic><topic>Coloring Agents</topic><topic>Cross-Linking Reagents</topic><topic>Dimethyl Sulfoxide</topic><topic>Dogs</topic><topic>Elastin</topic><topic>Electrophysiology</topic><topic>Femoral Artery</topic><topic>Femoral Vein</topic><topic>Iliac Artery</topic><topic>Iliac Vein</topic><topic>Matrix</topic><topic>Microscopy, Electron, Scanning</topic><topic>Prosthesis Design</topic><topic>Solvents</topic><topic>Thoracic Arteries</topic><topic>Tissue Preservation - methods</topic><topic>Vena Cava, Inferior</topic><topic>Vena Cava, Superior</topic><topic>Vessels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goissis, Gilberto</creatorcontrib><creatorcontrib>Suzigan, Sueli</creatorcontrib><creatorcontrib>Parreira, Diderot Rodrigues</creatorcontrib><creatorcontrib>Maniglia, José Vitor</creatorcontrib><creatorcontrib>Braile, Domingo Marcolino</creatorcontrib><creatorcontrib>Raymundo, Selma</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goissis, Gilberto</au><au>Suzigan, Sueli</au><au>Parreira, Diderot Rodrigues</au><au>Maniglia, José Vitor</au><au>Braile, Domingo Marcolino</au><au>Raymundo, Selma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and Characterization of Collagen-Elastin Matrices From Blood Vessels Intended as Small Diameter Vascular Grafts</atitle><jtitle>Artificial organs</jtitle><addtitle>Artificial Organs</addtitle><date>2000-03</date><risdate>2000</risdate><volume>24</volume><issue>3</issue><spage>217</spage><epage>223</epage><pages>217-223</pages><issn>0160-564X</issn><eissn>1525-1594</eissn><abstract>: This study describes the selective removal of cell and cell residues from small and large diameter blood vessels for the preparation of tubular collagen:elastin matrices intended for small diameter vascular prosthesis. The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. The efficiency of cell removal and the extent collagen and elastic fiber preservation were dependent on the segment and the type of blood vessel under study, with arteries characterized by a higher susceptibility of cell removal and better preservation of the collagen‐elastin matrix.</abstract><cop>Boston, MA, USA</cop><pub>Blackwell Science Inc</pub><pmid>10759645</pmid><doi>10.1046/j.1525-1594.2000.06537.x</doi><tpages>7</tpages></addata></record> |
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subjects | Acellular Alkalies Animals Aorta, Abdominal Biocompatible Materials Bioprosthesis Blood Vessel Prosthesis Blood Vessels - cytology Calorimetry, Differential Scanning Characterization Collagen Coloring Agents Cross-Linking Reagents Dimethyl Sulfoxide Dogs Elastin Electrophysiology Femoral Artery Femoral Vein Iliac Artery Iliac Vein Matrix Microscopy, Electron, Scanning Prosthesis Design Solvents Thoracic Arteries Tissue Preservation - methods Vena Cava, Inferior Vena Cava, Superior Vessels |
title | Preparation and Characterization of Collagen-Elastin Matrices From Blood Vessels Intended as Small Diameter Vascular Grafts |
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