Expression and regulation of growth-regulated oncogene α in human endometrial stromal cells
Growth-regulated oncogene α (GROα), a potent chemoattractant for neutrophils, has previously been detected in the endometrial stromal cells (ESC) of human endometrium. In this study, the mRNA expression of GROα in the endometrium was evaluated by reverse transcription–polymerase chain reaction analy...
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| Veröffentlicht in: | Molecular human reproduction 2001-08, Vol.7 (8), p.741-746 |
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| creator | Nasu, Kaei Fujisawa, Kayo Arima, Kazuyo Kai, Kengo Sugano, Terumasa Miyakawa, Isao |
| description | Growth-regulated oncogene α (GROα), a potent chemoattractant for neutrophils, has previously been detected in the endometrial stromal cells (ESC) of human endometrium. In this study, the mRNA expression of GROα in the endometrium was evaluated by reverse transcription–polymerase chain reaction analysis, while the localization of GROα protein was studied by immunohistochemistry and the concentrations of GROα were measured using an enzyme-linked immunosorbent assay (ELISA). The effects of known modulators of endometrial function on the production of GROα by ESC were also examined by ELISA and Northern blot analysis. The expression of both GROα mRNA and GROα protein was detected in the cycling endometrium. GROα protein was localized mainly in the stroma, and endometrial tissues in the secretory phase contained higher amounts of GROα protein than did those in the proliferative phase. The production of GROα by ESC was enhanced by in-vitro decidualization. Lipopolysaccharide, tumour necrosis factor-α and interleukin-1β also stimulated the expression of GROα mRNA and protein by ESC. These results suggest that the production of GROα by ESC is regulated by ovarian steroid hormones as well as by inflammatory mediators. The modulation of GROα concentrations in the local environment may contribute to normal and pathological processes in the uterus by regulating leukocyte trafficking in the endometrium. |
| doi_str_mv | 10.1093/molehr/7.8.741 |
| format | Article |
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In this study, the mRNA expression of GROα in the endometrium was evaluated by reverse transcription–polymerase chain reaction analysis, while the localization of GROα protein was studied by immunohistochemistry and the concentrations of GROα were measured using an enzyme-linked immunosorbent assay (ELISA). The effects of known modulators of endometrial function on the production of GROα by ESC were also examined by ELISA and Northern blot analysis. The expression of both GROα mRNA and GROα protein was detected in the cycling endometrium. GROα protein was localized mainly in the stroma, and endometrial tissues in the secretory phase contained higher amounts of GROα protein than did those in the proliferative phase. The production of GROα by ESC was enhanced by in-vitro decidualization. Lipopolysaccharide, tumour necrosis factor-α and interleukin-1β also stimulated the expression of GROα mRNA and protein by ESC. These results suggest that the production of GROα by ESC is regulated by ovarian steroid hormones as well as by inflammatory mediators. The modulation of GROα concentrations in the local environment may contribute to normal and pathological processes in the uterus by regulating leukocyte trafficking in the endometrium.</description><identifier>ISSN: 1360-9947</identifier><identifier>ISSN: 1460-2407</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/7.8.741</identifier><identifier>PMID: 11470861</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Cells, Cultured ; Chemokine CXCL1 ; Chemokines, CXC ; Chemotactic Factors - biosynthesis ; Chemotactic Factors - genetics ; Culture Media, Conditioned - chemistry ; cytokine network ; endometrial stromal cell ; Endometrium - cytology ; Endometrium - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Growth Substances - biosynthesis ; Growth Substances - genetics ; growth-regulated oncogene α ; Humans ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; Mammalian female genital system ; menstrual cycle ; Morphology. Physiology ; Premenopause - genetics ; Premenopause - metabolism ; RNA, Messenger - analysis ; RNA, Messenger - biosynthesis ; Stromal Cells - metabolism ; Vertebrates: reproduction</subject><ispartof>Molecular human reproduction, 2001-08, Vol.7 (8), p.741-746</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-82b9a5b1566e4d69409c23519a3ba1798ac3e40066a8e10547a95da5b865d3b73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1108914$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11470861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nasu, Kaei</creatorcontrib><creatorcontrib>Fujisawa, Kayo</creatorcontrib><creatorcontrib>Arima, Kazuyo</creatorcontrib><creatorcontrib>Kai, Kengo</creatorcontrib><creatorcontrib>Sugano, Terumasa</creatorcontrib><creatorcontrib>Miyakawa, Isao</creatorcontrib><title>Expression and regulation of growth-regulated oncogene α in human endometrial stromal cells</title><title>Molecular human reproduction</title><addtitle>Mol. Hum. Reprod</addtitle><description>Growth-regulated oncogene α (GROα), a potent chemoattractant for neutrophils, has previously been detected in the endometrial stromal cells (ESC) of human endometrium. In this study, the mRNA expression of GROα in the endometrium was evaluated by reverse transcription–polymerase chain reaction analysis, while the localization of GROα protein was studied by immunohistochemistry and the concentrations of GROα were measured using an enzyme-linked immunosorbent assay (ELISA). The effects of known modulators of endometrial function on the production of GROα by ESC were also examined by ELISA and Northern blot analysis. The expression of both GROα mRNA and GROα protein was detected in the cycling endometrium. GROα protein was localized mainly in the stroma, and endometrial tissues in the secretory phase contained higher amounts of GROα protein than did those in the proliferative phase. The production of GROα by ESC was enhanced by in-vitro decidualization. Lipopolysaccharide, tumour necrosis factor-α and interleukin-1β also stimulated the expression of GROα mRNA and protein by ESC. These results suggest that the production of GROα by ESC is regulated by ovarian steroid hormones as well as by inflammatory mediators. The modulation of GROα concentrations in the local environment may contribute to normal and pathological processes in the uterus by regulating leukocyte trafficking in the endometrium.</description><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chemokine CXCL1</subject><subject>Chemokines, CXC</subject><subject>Chemotactic Factors - biosynthesis</subject><subject>Chemotactic Factors - genetics</subject><subject>Culture Media, Conditioned - chemistry</subject><subject>cytokine network</subject><subject>endometrial stromal cell</subject><subject>Endometrium - cytology</subject><subject>Endometrium - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Growth Substances - biosynthesis</subject><subject>Growth Substances - genetics</subject><subject>growth-regulated oncogene α</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Mammalian female genital system</subject><subject>menstrual cycle</subject><subject>Morphology. Physiology</subject><subject>Premenopause - genetics</subject><subject>Premenopause - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Stromal Cells - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>1360-9947</issn><issn>1460-2407</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM1u1DAUha2qiJbCtkvkRcUuU3v8vyyltFSV2LQSQkjWTXJnJjSxp3YiymPxIjwTHk0ErM79-c7V1SHklLMFZ06cD7HHTTo3C7swkh-QYy41q5aSmcNSi1I7J80ReZXzd8a4WSr7khxxLg2zmh-Tb1fP24Q5dzFQCC1NuJ56GHdtXNF1ij_GTTUPsaUxNHGNAenvX7QLdDMNECiGNg44pg56mscUh6IN9n1-TV6soM_4ZtYT8vDx6v7yprr7fP3p8uKuaqSWY2WXtQNVc6U1ylY7yVyzFIo7EDVw4yw0AiVjWoNFzpQ04FRbHFarVtRGnJB3-7vbFJ8mzKMfurz7AALGKXvDmZCM8wIu9mCTYs4JV36bugHST8-Z3-Xp93l6460veRbD2_nyVA_Y_sPnAAtwNgOQG-hXCULT5f84Zh2XBav2WJdHfP67hvTotRFG-ZsvX_2tfH97_UEZL8QfRd-PMw</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Nasu, Kaei</creator><creator>Fujisawa, Kayo</creator><creator>Arima, Kazuyo</creator><creator>Kai, Kengo</creator><creator>Sugano, Terumasa</creator><creator>Miyakawa, Isao</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Expression and regulation of growth-regulated oncogene α in human endometrial stromal cells</title><author>Nasu, Kaei ; Fujisawa, Kayo ; Arima, Kazuyo ; Kai, Kengo ; Sugano, Terumasa ; Miyakawa, Isao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-82b9a5b1566e4d69409c23519a3ba1798ac3e40066a8e10547a95da5b865d3b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chemokine CXCL1</topic><topic>Chemokines, CXC</topic><topic>Chemotactic Factors - biosynthesis</topic><topic>Chemotactic Factors - genetics</topic><topic>Culture Media, Conditioned - chemistry</topic><topic>cytokine network</topic><topic>endometrial stromal cell</topic><topic>Endometrium - cytology</topic><topic>Endometrium - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Growth Substances - biosynthesis</topic><topic>Growth Substances - genetics</topic><topic>growth-regulated oncogene α</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>Mammalian female genital system</topic><topic>menstrual cycle</topic><topic>Morphology. Physiology</topic><topic>Premenopause - genetics</topic><topic>Premenopause - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Stromal Cells - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nasu, Kaei</creatorcontrib><creatorcontrib>Fujisawa, Kayo</creatorcontrib><creatorcontrib>Arima, Kazuyo</creatorcontrib><creatorcontrib>Kai, Kengo</creatorcontrib><creatorcontrib>Sugano, Terumasa</creatorcontrib><creatorcontrib>Miyakawa, Isao</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nasu, Kaei</au><au>Fujisawa, Kayo</au><au>Arima, Kazuyo</au><au>Kai, Kengo</au><au>Sugano, Terumasa</au><au>Miyakawa, Isao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and regulation of growth-regulated oncogene α in human endometrial stromal cells</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol. Hum. Reprod</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>7</volume><issue>8</issue><spage>741</spage><epage>746</epage><pages>741-746</pages><issn>1360-9947</issn><issn>1460-2407</issn><eissn>1460-2407</eissn><abstract>Growth-regulated oncogene α (GROα), a potent chemoattractant for neutrophils, has previously been detected in the endometrial stromal cells (ESC) of human endometrium. In this study, the mRNA expression of GROα in the endometrium was evaluated by reverse transcription–polymerase chain reaction analysis, while the localization of GROα protein was studied by immunohistochemistry and the concentrations of GROα were measured using an enzyme-linked immunosorbent assay (ELISA). The effects of known modulators of endometrial function on the production of GROα by ESC were also examined by ELISA and Northern blot analysis. The expression of both GROα mRNA and GROα protein was detected in the cycling endometrium. GROα protein was localized mainly in the stroma, and endometrial tissues in the secretory phase contained higher amounts of GROα protein than did those in the proliferative phase. The production of GROα by ESC was enhanced by in-vitro decidualization. Lipopolysaccharide, tumour necrosis factor-α and interleukin-1β also stimulated the expression of GROα mRNA and protein by ESC. These results suggest that the production of GROα by ESC is regulated by ovarian steroid hormones as well as by inflammatory mediators. The modulation of GROα concentrations in the local environment may contribute to normal and pathological processes in the uterus by regulating leukocyte trafficking in the endometrium.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11470861</pmid><doi>10.1093/molehr/7.8.741</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Cells, Cultured Chemokine CXCL1 Chemokines, CXC Chemotactic Factors - biosynthesis Chemotactic Factors - genetics Culture Media, Conditioned - chemistry cytokine network endometrial stromal cell Endometrium - cytology Endometrium - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation Growth Substances - biosynthesis Growth Substances - genetics growth-regulated oncogene α Humans Immunohistochemistry Intercellular Signaling Peptides and Proteins Mammalian female genital system menstrual cycle Morphology. Physiology Premenopause - genetics Premenopause - metabolism RNA, Messenger - analysis RNA, Messenger - biosynthesis Stromal Cells - metabolism Vertebrates: reproduction |
| title | Expression and regulation of growth-regulated oncogene α in human endometrial stromal cells |
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