Calcium buffering in coronary smooth muscle after chronic occlusion and exercise training
Exercise promotes "sarcoplasmic reticulum (SR) Ca2+ unloading" in porcine coronary smooth muscle, resulting in decreased agonist-induced Ca2+ release. We studied Ca2+ handling in healthy, non-occluded right coronary artery cells from hearts chronically occluded at the circumflex artery. My...
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| Veröffentlicht in: | Cardiovascular research 2001-08, Vol.51 (2), p.359-367 |
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| creator | JONES, Joyce J DIETZ, Nancy J HEAPS, Cristine L PARKER, Janet L STUREK, Michael |
| description | Exercise promotes "sarcoplasmic reticulum (SR) Ca2+ unloading" in porcine coronary smooth muscle, resulting in decreased agonist-induced Ca2+ release. We studied Ca2+ handling in healthy, non-occluded right coronary artery cells from hearts chronically occluded at the circumflex artery.
Myoplasmic free Ca2+ (Ca(m)) was assessed with fura-2 in cells from sedentary (n=8) and aerobically exercise-trained (n=6) female Yucatan pigs after 6-month circumflex artery ameroid occlusion (OCC) and in cells from non-occluded, sedentary pigs (SED, n=5). First, Ca influx was induced by 80 mM KCl depolarization (priming step) followed by 5 mM caffeine to elicit maximal Ca2+ release and depletion. The SR was Ca-loaded again by depolarization and then exposed to caffeine after 2- or 11-min recovery to compare SR Ca2+ unloading.
Baseline Ca(m), caffeine-induced peak Ca(m), and depolarization-induced maximum Ca(m) were decreased, and depolarization-induced time-to-half-maximum was increased in OCC vs. SED pigs, suggesting a tonic Ca2+ buffering (lowering) effect of occlusion. Exercise did not alter these effects. SR Ca2+ unloading occurred only in SED, as evidenced by decreased caffeine-induced Ca2+ release after 11 min of recovery, and was inhibited by low extracellular Na+.
SR Ca2+ unloading can be demonstrated in coronary smooth muscle from sedentary pigs using a novel SR Ca2+ unloading protocol, and Ca2+ unloading partly depends on Na+-Ca2+ exchange activity. Furthermore, SR Ca2+ unloading in cells from non-occluded right coronary arteries of chronically circumflex-occluded pig hearts was not altered by exercise, perhaps due to enhanced tonic Ca2+ extrusion versus cells from normal, sedentary animals. |
| doi_str_mv | 10.1016/S0008-6363(01)00305-4 |
| format | Article |
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Myoplasmic free Ca2+ (Ca(m)) was assessed with fura-2 in cells from sedentary (n=8) and aerobically exercise-trained (n=6) female Yucatan pigs after 6-month circumflex artery ameroid occlusion (OCC) and in cells from non-occluded, sedentary pigs (SED, n=5). First, Ca influx was induced by 80 mM KCl depolarization (priming step) followed by 5 mM caffeine to elicit maximal Ca2+ release and depletion. The SR was Ca-loaded again by depolarization and then exposed to caffeine after 2- or 11-min recovery to compare SR Ca2+ unloading.
Baseline Ca(m), caffeine-induced peak Ca(m), and depolarization-induced maximum Ca(m) were decreased, and depolarization-induced time-to-half-maximum was increased in OCC vs. SED pigs, suggesting a tonic Ca2+ buffering (lowering) effect of occlusion. Exercise did not alter these effects. SR Ca2+ unloading occurred only in SED, as evidenced by decreased caffeine-induced Ca2+ release after 11 min of recovery, and was inhibited by low extracellular Na+.
SR Ca2+ unloading can be demonstrated in coronary smooth muscle from sedentary pigs using a novel SR Ca2+ unloading protocol, and Ca2+ unloading partly depends on Na+-Ca2+ exchange activity. Furthermore, SR Ca2+ unloading in cells from non-occluded right coronary arteries of chronically circumflex-occluded pig hearts was not altered by exercise, perhaps due to enhanced tonic Ca2+ extrusion versus cells from normal, sedentary animals.</description><identifier>ISSN: 0008-6363</identifier><identifier>EISSN: 1755-3245</identifier><identifier>DOI: 10.1016/S0008-6363(01)00305-4</identifier><identifier>PMID: 11470476</identifier><identifier>CODEN: CVREAU</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adaptation, Physiological ; Analysis of Variance ; Animals ; Biological and medical sciences ; Buffers ; Caffeine - pharmacology ; Calcium - metabolism ; Cardiology. Vascular system ; Cells, Cultured ; Coronary Disease - metabolism ; Coronary heart disease ; Coronary Vessels - drug effects ; Female ; Heart ; Medical sciences ; Models, Animal ; Muscle, Smooth, Vascular - metabolism ; Physical Conditioning, Animal ; Potassium Chloride - pharmacology ; Sarcoplasmic Reticulum - metabolism ; Sodium-Calcium Exchanger - metabolism ; Swine, Miniature</subject><ispartof>Cardiovascular research, 2001-08, Vol.51 (2), p.359-367</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-5d72e87b1c80acfde2b0adf0292707df5d471a55c66e28058c796a2c3b8f032d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14057466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11470476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JONES, Joyce J</creatorcontrib><creatorcontrib>DIETZ, Nancy J</creatorcontrib><creatorcontrib>HEAPS, Cristine L</creatorcontrib><creatorcontrib>PARKER, Janet L</creatorcontrib><creatorcontrib>STUREK, Michael</creatorcontrib><title>Calcium buffering in coronary smooth muscle after chronic occlusion and exercise training</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Exercise promotes "sarcoplasmic reticulum (SR) Ca2+ unloading" in porcine coronary smooth muscle, resulting in decreased agonist-induced Ca2+ release. We studied Ca2+ handling in healthy, non-occluded right coronary artery cells from hearts chronically occluded at the circumflex artery.
Myoplasmic free Ca2+ (Ca(m)) was assessed with fura-2 in cells from sedentary (n=8) and aerobically exercise-trained (n=6) female Yucatan pigs after 6-month circumflex artery ameroid occlusion (OCC) and in cells from non-occluded, sedentary pigs (SED, n=5). First, Ca influx was induced by 80 mM KCl depolarization (priming step) followed by 5 mM caffeine to elicit maximal Ca2+ release and depletion. The SR was Ca-loaded again by depolarization and then exposed to caffeine after 2- or 11-min recovery to compare SR Ca2+ unloading.
Baseline Ca(m), caffeine-induced peak Ca(m), and depolarization-induced maximum Ca(m) were decreased, and depolarization-induced time-to-half-maximum was increased in OCC vs. SED pigs, suggesting a tonic Ca2+ buffering (lowering) effect of occlusion. Exercise did not alter these effects. SR Ca2+ unloading occurred only in SED, as evidenced by decreased caffeine-induced Ca2+ release after 11 min of recovery, and was inhibited by low extracellular Na+.
SR Ca2+ unloading can be demonstrated in coronary smooth muscle from sedentary pigs using a novel SR Ca2+ unloading protocol, and Ca2+ unloading partly depends on Na+-Ca2+ exchange activity. Furthermore, SR Ca2+ unloading in cells from non-occluded right coronary arteries of chronically circumflex-occluded pig hearts was not altered by exercise, perhaps due to enhanced tonic Ca2+ extrusion versus cells from normal, sedentary animals.</description><subject>Adaptation, Physiological</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Cardiology. Vascular system</subject><subject>Cells, Cultured</subject><subject>Coronary Disease - metabolism</subject><subject>Coronary heart disease</subject><subject>Coronary Vessels - drug effects</subject><subject>Female</subject><subject>Heart</subject><subject>Medical sciences</subject><subject>Models, Animal</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Physical Conditioning, Animal</subject><subject>Potassium Chloride - pharmacology</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Sodium-Calcium Exchanger - metabolism</subject><subject>Swine, Miniature</subject><issn>0008-6363</issn><issn>1755-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFKJDEQhoMoOuo-gpKLoofWStJJ2qMMuyoMeFj3sKeQrk6cSHdHk25Y337jOOipKOr7q6iPkBMGVwyYuv4NAE2lhBIXwC4BBMiq3iELpqWsBK_lLll8IQfkMOeX0kqp631ywFitodZqQf4ubY9hHmg7e-9SGJ9pGCnGFEeb3mkeYpzWdJgz9o5aP7lEcV2GAWlE7Occ4kjt2FH3zyUM2dEp2TCWPcdkz9s-ux_bekT-_Pr5tLyvVo93D8vbVYWi4VMlO81do1uGDVj0neMt2M4Dv-EadOdlV2tmpUSlHG9ANqhvlOUo2saD4J04Iuefe19TfJtdnswQMrq-t6OLczaaFYwxVkD5CWKKOSfnzWsKQ_nSMDAfTs3GqfkQZoCZjVNTl9zp9sDcDq77Tm0lFuBsC9iMtvfJjsXEN1dDsa6U-A9n6n_q</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>JONES, Joyce J</creator><creator>DIETZ, Nancy J</creator><creator>HEAPS, Cristine L</creator><creator>PARKER, Janet L</creator><creator>STUREK, Michael</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Calcium buffering in coronary smooth muscle after chronic occlusion and exercise training</title><author>JONES, Joyce J ; DIETZ, Nancy J ; HEAPS, Cristine L ; PARKER, Janet L ; STUREK, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-5d72e87b1c80acfde2b0adf0292707df5d471a55c66e28058c796a2c3b8f032d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adaptation, Physiological</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Cardiology. Vascular system</topic><topic>Cells, Cultured</topic><topic>Coronary Disease - metabolism</topic><topic>Coronary heart disease</topic><topic>Coronary Vessels - drug effects</topic><topic>Female</topic><topic>Heart</topic><topic>Medical sciences</topic><topic>Models, Animal</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Physical Conditioning, Animal</topic><topic>Potassium Chloride - pharmacology</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Sodium-Calcium Exchanger - metabolism</topic><topic>Swine, Miniature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JONES, Joyce J</creatorcontrib><creatorcontrib>DIETZ, Nancy J</creatorcontrib><creatorcontrib>HEAPS, Cristine L</creatorcontrib><creatorcontrib>PARKER, Janet L</creatorcontrib><creatorcontrib>STUREK, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JONES, Joyce J</au><au>DIETZ, Nancy J</au><au>HEAPS, Cristine L</au><au>PARKER, Janet L</au><au>STUREK, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium buffering in coronary smooth muscle after chronic occlusion and exercise training</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>51</volume><issue>2</issue><spage>359</spage><epage>367</epage><pages>359-367</pages><issn>0008-6363</issn><eissn>1755-3245</eissn><coden>CVREAU</coden><abstract>Exercise promotes "sarcoplasmic reticulum (SR) Ca2+ unloading" in porcine coronary smooth muscle, resulting in decreased agonist-induced Ca2+ release. We studied Ca2+ handling in healthy, non-occluded right coronary artery cells from hearts chronically occluded at the circumflex artery.
Myoplasmic free Ca2+ (Ca(m)) was assessed with fura-2 in cells from sedentary (n=8) and aerobically exercise-trained (n=6) female Yucatan pigs after 6-month circumflex artery ameroid occlusion (OCC) and in cells from non-occluded, sedentary pigs (SED, n=5). First, Ca influx was induced by 80 mM KCl depolarization (priming step) followed by 5 mM caffeine to elicit maximal Ca2+ release and depletion. The SR was Ca-loaded again by depolarization and then exposed to caffeine after 2- or 11-min recovery to compare SR Ca2+ unloading.
Baseline Ca(m), caffeine-induced peak Ca(m), and depolarization-induced maximum Ca(m) were decreased, and depolarization-induced time-to-half-maximum was increased in OCC vs. SED pigs, suggesting a tonic Ca2+ buffering (lowering) effect of occlusion. Exercise did not alter these effects. SR Ca2+ unloading occurred only in SED, as evidenced by decreased caffeine-induced Ca2+ release after 11 min of recovery, and was inhibited by low extracellular Na+.
SR Ca2+ unloading can be demonstrated in coronary smooth muscle from sedentary pigs using a novel SR Ca2+ unloading protocol, and Ca2+ unloading partly depends on Na+-Ca2+ exchange activity. Furthermore, SR Ca2+ unloading in cells from non-occluded right coronary arteries of chronically circumflex-occluded pig hearts was not altered by exercise, perhaps due to enhanced tonic Ca2+ extrusion versus cells from normal, sedentary animals.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11470476</pmid><doi>10.1016/S0008-6363(01)00305-4</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptation, Physiological Analysis of Variance Animals Biological and medical sciences Buffers Caffeine - pharmacology Calcium - metabolism Cardiology. Vascular system Cells, Cultured Coronary Disease - metabolism Coronary heart disease Coronary Vessels - drug effects Female Heart Medical sciences Models, Animal Muscle, Smooth, Vascular - metabolism Physical Conditioning, Animal Potassium Chloride - pharmacology Sarcoplasmic Reticulum - metabolism Sodium-Calcium Exchanger - metabolism Swine, Miniature |
| title | Calcium buffering in coronary smooth muscle after chronic occlusion and exercise training |
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