Substantial in vivo proliferation of CD4+ and CD8+ T lymphocytes during secondary Listeria monocytogenes infection
In mice Listeria monocytogenes infection induces a strong T cell response. In an attempt to quantitatively analyze the magnitude and kinetics of the CD4+ and CD8+ T cell response during L. monocytogenes infection in vivo we used a T cell transfer system that is independent of in vitro cell culture t...
Gespeichert in:
| Veröffentlicht in: | European journal of immunology 2000-04, Vol.30 (4), p.1053-1059 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1059 |
|---|---|
| container_issue | 4 |
| container_start_page | 1053 |
| container_title | European journal of immunology |
| container_volume | 30 |
| creator | Mittrücker, Hans‐Willi Köhler, Anne Kaufmann, Stefan H. E. |
| description | In mice Listeria monocytogenes infection induces a strong T cell response. In an attempt to quantitatively analyze the magnitude and kinetics of the CD4+ and CD8+ T cell response during L. monocytogenes infection in vivo we used a T cell transfer system that is independent of in vitro cell culture techniques and information about the identity of immunogenic T cell epitopes. Our results demonstrate substantial expansion of the in vivo primed and transferred T cell populations in response to L. monocytogenes. At the peak of response, transferred T cells represented more than one third of the total CD4+ and CD8+ T cell populations in blood and spleen of recipient mice. After stimulation in vitro, 40 % of these CD4+ T cells responded to heat‐killed listeriae with the production of IFN‐γ. Thus, our results reveal that in addition to the large CD8+ T cell population an almost equally large population of Listeria‐reactive CD4+ T cells is generated in response to L. monocytogenes infection. |
| doi_str_mv | 10.1002/(SICI)1521-4141(200004)30:4<1053::AID-IMMU1053>3.0.CO;2-N |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71031968</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71031968</sourcerecordid><originalsourceid>FETCH-LOGICAL-p2933-1322773e1fa3aac8585bc3cd4cab8feb04412334c6cdce0937b8009f735909613</originalsourceid><addsrcrecordid>eNpNkV1r2zAYhcXYWLNuf2HoarQUZ5JeObazMQjuPgxpc9H2crzIstxp2FJm2R3595NJW6YbfT3vgXMOIRvOlpwx8fHspiqrc54Knkgu-ZlgcclzYGv5mbMU1utNdZlUV1d38-0LLNmy3H0SyfULsnieekkWjHGZiCJnJ-RNCL-jSLFKi9fkhLNsxbICFmS4meowKjda1VHr6IN98HQ_-M62ZlCj9Y76lpaX8oIq18RDfkFvaXfo97-8Powm0GYarLunwWjvGjUc6NaG0QxW0d67mfH3xkXOutboWfAtedWqLph3j_spufv29bb8kWx336tys032ogBIOAiRZWB4q0Apnad5WmvQjdSqzltTMym5AJB6pRttWAFZnUeDbQZpEX1yOCUfjrrRzp_JhBF7G7TpOuWMnwJmnAEvVnkE3z-CU92bBveD7aMRfEopAj-PwF_bmcN__zjXhXNbOOeOc-54bAuBocS5H4xl4VNZCMiw3KHA6-c3-AdoZY4M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71031968</pqid></control><display><type>article</type><title>Substantial in vivo proliferation of CD4+ and CD8+ T lymphocytes during secondary Listeria monocytogenes infection</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley Free Content</source><source>Wiley Online Library All Journals</source><creator>Mittrücker, Hans‐Willi ; Köhler, Anne ; Kaufmann, Stefan H. E.</creator><creatorcontrib>Mittrücker, Hans‐Willi ; Köhler, Anne ; Kaufmann, Stefan H. E.</creatorcontrib><description>In mice Listeria monocytogenes infection induces a strong T cell response. In an attempt to quantitatively analyze the magnitude and kinetics of the CD4+ and CD8+ T cell response during L. monocytogenes infection in vivo we used a T cell transfer system that is independent of in vitro cell culture techniques and information about the identity of immunogenic T cell epitopes. Our results demonstrate substantial expansion of the in vivo primed and transferred T cell populations in response to L. monocytogenes. At the peak of response, transferred T cells represented more than one third of the total CD4+ and CD8+ T cell populations in blood and spleen of recipient mice. After stimulation in vitro, 40 % of these CD4+ T cells responded to heat‐killed listeriae with the production of IFN‐γ. Thus, our results reveal that in addition to the large CD8+ T cell population an almost equally large population of Listeria‐reactive CD4+ T cells is generated in response to L. monocytogenes infection.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/(SICI)1521-4141(200004)30:4<1053::AID-IMMU1053>3.0.CO;2-N</identifier><identifier>PMID: 10760793</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH</publisher><subject>Adoptive Transfer ; AIDS/HIV ; Animals ; CD4-Positive T-Lymphocytes - cytology ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - metabolism ; CD8-Positive T-Lymphocytes - cytology ; CD8-Positive T-Lymphocytes - immunology ; Cell Division ; Cells, Cultured ; Epitopes, T-Lymphocyte - immunology ; Female ; Flow Cytometry ; Hot Temperature ; Interferon-gamma - biosynthesis ; Kinetics ; Listeria monocytogenes ; Listeria monocytogenes - immunology ; Listeriosis - immunology ; Listeriosis - microbiology ; Listeriosis - pathology ; Lymphocyte Activation - immunology ; Lymphocyte Count ; Male ; Mice ; Mice, Inbred C57BL ; Spleen - immunology ; Spleen - microbiology ; T lymphocyte</subject><ispartof>European journal of immunology, 2000-04, Vol.30 (4), p.1053-1059</ispartof><rights>2000 WILEY‐VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291521-4141%28200004%2930%3A4%3C1053%3A%3AAID-IMMU1053%3E3.0.CO%3B2-N$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291521-4141%28200004%2930%3A4%3C1053%3A%3AAID-IMMU1053%3E3.0.CO%3B2-N$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10760793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mittrücker, Hans‐Willi</creatorcontrib><creatorcontrib>Köhler, Anne</creatorcontrib><creatorcontrib>Kaufmann, Stefan H. E.</creatorcontrib><title>Substantial in vivo proliferation of CD4+ and CD8+ T lymphocytes during secondary Listeria monocytogenes infection</title><title>European journal of immunology</title><addtitle>Eur J Immunol</addtitle><description>In mice Listeria monocytogenes infection induces a strong T cell response. In an attempt to quantitatively analyze the magnitude and kinetics of the CD4+ and CD8+ T cell response during L. monocytogenes infection in vivo we used a T cell transfer system that is independent of in vitro cell culture techniques and information about the identity of immunogenic T cell epitopes. Our results demonstrate substantial expansion of the in vivo primed and transferred T cell populations in response to L. monocytogenes. At the peak of response, transferred T cells represented more than one third of the total CD4+ and CD8+ T cell populations in blood and spleen of recipient mice. After stimulation in vitro, 40 % of these CD4+ T cells responded to heat‐killed listeriae with the production of IFN‐γ. Thus, our results reveal that in addition to the large CD8+ T cell population an almost equally large population of Listeria‐reactive CD4+ T cells is generated in response to L. monocytogenes infection.</description><subject>Adoptive Transfer</subject><subject>AIDS/HIV</subject><subject>Animals</subject><subject>CD4-Positive T-Lymphocytes - cytology</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>CD8-Positive T-Lymphocytes - cytology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Epitopes, T-Lymphocyte - immunology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Hot Temperature</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Kinetics</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - immunology</subject><subject>Listeriosis - immunology</subject><subject>Listeriosis - microbiology</subject><subject>Listeriosis - pathology</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Count</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Spleen - immunology</subject><subject>Spleen - microbiology</subject><subject>T lymphocyte</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkV1r2zAYhcXYWLNuf2HoarQUZ5JeObazMQjuPgxpc9H2crzIstxp2FJm2R3595NJW6YbfT3vgXMOIRvOlpwx8fHspiqrc54Knkgu-ZlgcclzYGv5mbMU1utNdZlUV1d38-0LLNmy3H0SyfULsnieekkWjHGZiCJnJ-RNCL-jSLFKi9fkhLNsxbICFmS4meowKjda1VHr6IN98HQ_-M62ZlCj9Y76lpaX8oIq18RDfkFvaXfo97-8Powm0GYarLunwWjvGjUc6NaG0QxW0d67mfH3xkXOutboWfAtedWqLph3j_spufv29bb8kWx336tys032ogBIOAiRZWB4q0Apnad5WmvQjdSqzltTMym5AJB6pRttWAFZnUeDbQZpEX1yOCUfjrrRzp_JhBF7G7TpOuWMnwJmnAEvVnkE3z-CU92bBveD7aMRfEopAj-PwF_bmcN__zjXhXNbOOeOc-54bAuBocS5H4xl4VNZCMiw3KHA6-c3-AdoZY4M</recordid><startdate>200004</startdate><enddate>200004</enddate><creator>Mittrücker, Hans‐Willi</creator><creator>Köhler, Anne</creator><creator>Kaufmann, Stefan H. E.</creator><general>WILEY‐VCH Verlag GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200004</creationdate><title>Substantial in vivo proliferation of CD4+ and CD8+ T lymphocytes during secondary Listeria monocytogenes infection</title><author>Mittrücker, Hans‐Willi ; Köhler, Anne ; Kaufmann, Stefan H. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2933-1322773e1fa3aac8585bc3cd4cab8feb04412334c6cdce0937b8009f735909613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adoptive Transfer</topic><topic>AIDS/HIV</topic><topic>Animals</topic><topic>CD4-Positive T-Lymphocytes - cytology</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>CD8-Positive T-Lymphocytes - cytology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Epitopes, T-Lymphocyte - immunology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Hot Temperature</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Kinetics</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - immunology</topic><topic>Listeriosis - immunology</topic><topic>Listeriosis - microbiology</topic><topic>Listeriosis - pathology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Count</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Spleen - immunology</topic><topic>Spleen - microbiology</topic><topic>T lymphocyte</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mittrücker, Hans‐Willi</creatorcontrib><creatorcontrib>Köhler, Anne</creatorcontrib><creatorcontrib>Kaufmann, Stefan H. E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mittrücker, Hans‐Willi</au><au>Köhler, Anne</au><au>Kaufmann, Stefan H. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Substantial in vivo proliferation of CD4+ and CD8+ T lymphocytes during secondary Listeria monocytogenes infection</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>2000-04</date><risdate>2000</risdate><volume>30</volume><issue>4</issue><spage>1053</spage><epage>1059</epage><pages>1053-1059</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><abstract>In mice Listeria monocytogenes infection induces a strong T cell response. In an attempt to quantitatively analyze the magnitude and kinetics of the CD4+ and CD8+ T cell response during L. monocytogenes infection in vivo we used a T cell transfer system that is independent of in vitro cell culture techniques and information about the identity of immunogenic T cell epitopes. Our results demonstrate substantial expansion of the in vivo primed and transferred T cell populations in response to L. monocytogenes. At the peak of response, transferred T cells represented more than one third of the total CD4+ and CD8+ T cell populations in blood and spleen of recipient mice. After stimulation in vitro, 40 % of these CD4+ T cells responded to heat‐killed listeriae with the production of IFN‐γ. Thus, our results reveal that in addition to the large CD8+ T cell population an almost equally large population of Listeria‐reactive CD4+ T cells is generated in response to L. monocytogenes infection.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><pmid>10760793</pmid><doi>10.1002/(SICI)1521-4141(200004)30:4<1053::AID-IMMU1053>3.0.CO;2-N</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2980 |
| ispartof | European journal of immunology, 2000-04, Vol.30 (4), p.1053-1059 |
| issn | 0014-2980 1521-4141 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71031968 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Wiley Online Library All Journals |
| subjects | Adoptive Transfer AIDS/HIV Animals CD4-Positive T-Lymphocytes - cytology CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism CD8-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - immunology Cell Division Cells, Cultured Epitopes, T-Lymphocyte - immunology Female Flow Cytometry Hot Temperature Interferon-gamma - biosynthesis Kinetics Listeria monocytogenes Listeria monocytogenes - immunology Listeriosis - immunology Listeriosis - microbiology Listeriosis - pathology Lymphocyte Activation - immunology Lymphocyte Count Male Mice Mice, Inbred C57BL Spleen - immunology Spleen - microbiology T lymphocyte |
| title | Substantial in vivo proliferation of CD4+ and CD8+ T lymphocytes during secondary Listeria monocytogenes infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T17%3A54%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Substantial%20in%20vivo%20proliferation%20of%20CD4+%20and%20CD8+%20T%20lymphocytes%20during%20secondary%20Listeria%20monocytogenes%20infection&rft.jtitle=European%20journal%20of%20immunology&rft.au=Mittr%C3%BCcker,%20Hans%E2%80%90Willi&rft.date=2000-04&rft.volume=30&rft.issue=4&rft.spage=1053&rft.epage=1059&rft.pages=1053-1059&rft.issn=0014-2980&rft.eissn=1521-4141&rft_id=info:doi/10.1002/(SICI)1521-4141(200004)30:4%3C1053::AID-IMMU1053%3E3.0.CO;2-N&rft_dat=%3Cproquest_pubme%3E71031968%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71031968&rft_id=info:pmid/10760793&rfr_iscdi=true |