High-Throughput Proteomics Using High-Efficiency Multiple-Capillary Liquid Chromatography with On-Line High-Performance ESI FTICR Mass Spectrometry

We report on the design and application of a high-efficiency multiple-capillary liquid chromatography (LC) system for high-throughput proteome analysis. The multiple-capillary LC system using commercial LC pumps was operated at a pressure of 10 000 psi to deliver mobile phases through a novel passiv...

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Veröffentlicht in:	Analytical chemistry (Washington) 2001-07, Vol.73 (13), p.3011-3021
Hauptverfasser:	Shen, Yufeng, Tolić, Nikola, Zhao, Rui, Paša-Tolić, Ljiljana, Li, Lingjun, Berger, Scott J, Harkewicz, Richard, Anderson, Gordon A, Belov, Mikhail E, Smith, Richard D
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography Chromatography, Liquid - methods Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry General aspects, investigation methods Peptide Mapping Proteins Proteome Reproducibility of Results Saccharomyces cerevisiae - chemistry Spectrometry, Mass, Electrospray Ionization - methods Spectrophotometry, Ultraviolet Spectroscopy, Fourier Transform Infrared Spectrum analysis Trypsin - chemistry
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