Differential leucocyte subpopulation analysis of leucodepleted red cell products
Donor leucocytes are responsible for many adverse transfusion effects. Clinical reactions may be attributed to specific leucocyte subsets. In this study leucocyte subpopulations were identified and quantified pre‐ and post‐leucodepletion by integral filtration using novel Optipac® configurations inc...
Gespeichert in:
| Veröffentlicht in: | Transfusion medicine (Oxford, England) England), 2000-03, Vol.10 (1), p.49-58 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 58 |
|---|---|
| container_issue | 1 |
| container_start_page | 49 |
| container_title | Transfusion medicine (Oxford, England) |
| container_volume | 10 |
| creator | Rider, J R Want, E J Winter, M A Turton, J R Pamphilon, D H Nobes, P |
| description | Donor leucocytes are responsible for many adverse transfusion effects. Clinical reactions may be attributed to specific leucocyte subsets. In this study leucocyte subpopulations were identified and quantified pre‐ and post‐leucodepletion by integral filtration using novel Optipac® configurations incorporating either WBF‐1 (Pall Medsep) or RS2000 (Asahi) whole blood filters. Leucocytes were analysed by flow cytometry using direct, four‐colour, membrane immunofluorescence with monoclonal antibodies specific for CD 3, 14, 16, 19 and 45. Filtration reduced the leucocyte load by 3–4 log10, consistently giving products with |
| doi_str_mv | 10.1046/j.1365-3148.2000.00231.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71029253</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71029253</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4021-4b05ef84a90d78ef4c226b54d5b494aed52b9e958f39b49dc798dc4f5bd831283</originalsourceid><addsrcrecordid>eNqNkE1v1DAQhi1URJeWv4By4pYw_kociQvqxxYopVJb9Wg59ljK4t2kcaLu_nscUlUcOVi27OedGT-EZBQKCqL8vCkoL2XOqVAFA4ACgHFa7N-Q1evDEVlBLVVeyUodk_cxbgAoZzV7R44pVCUw4Ctye956jwPuxtaELOBkO3sYMYtT03f9FMzYdrvM7Ew4xDZmnV8Yh33AEV02pGUxhKwfOjfZMZ6St96EiB9e9hPycHlxf3aVX_9afzv7ep1bAYzmogGJXglTg6sUemEZKxspnGxELQw6yZoa0_ye1-nG2apWzgovG6c4ZYqfkE9L3dT4acI46m0b50nMDrsp6opC-qvkCVQLaIcuxgG97od2a4aDpqBnm3qjZ2l6lqZnm_qvTb1P0Y8vPaZmi-6f4KIvAV8W4LkNePjvwvr-50U6pHi-xNs44v41bobfuqx4JfXjzVrf3P34fr6-rXXJ_wDOaJNa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71029253</pqid></control><display><type>article</type><title>Differential leucocyte subpopulation analysis of leucodepleted red cell products</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Rider, J R ; Want, E J ; Winter, M A ; Turton, J R ; Pamphilon, D H ; Nobes, P</creator><creatorcontrib>Rider, J R ; Want, E J ; Winter, M A ; Turton, J R ; Pamphilon, D H ; Nobes, P</creatorcontrib><description>Donor leucocytes are responsible for many adverse transfusion effects. Clinical reactions may be attributed to specific leucocyte subsets. In this study leucocyte subpopulations were identified and quantified pre‐ and post‐leucodepletion by integral filtration using novel Optipac® configurations incorporating either WBF‐1 (Pall Medsep) or RS2000 (Asahi) whole blood filters. Leucocytes were analysed by flow cytometry using direct, four‐colour, membrane immunofluorescence with monoclonal antibodies specific for CD 3, 14, 16, 19 and 45. Filtration reduced the leucocyte load by 3–4 log10, consistently giving products with < 2 cells μL−1. Subset distributions were also affected with the proportion of neutrophils and monocytes increased and the lymphocyte/monocyte ratio inverted. These effects were independent of the preprocessing hold conditions, filter used and buffy coat (BC) removal. All filtered red cell products contained 75–80% neutrophils, 16–20% monocytes and 2–7% lymphocytes. Results presented here demonstrate that whole blood filtration, and BC removal, significantly reduce the content and substantially alter the subpopulation distribution of the donor leucocytes remaining in leucodepleted red cell products.</description><identifier>ISSN: 0958-7578</identifier><identifier>EISSN: 1365-3148</identifier><identifier>DOI: 10.1046/j.1365-3148.2000.00231.x</identifier><identifier>PMID: 10760203</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>blood ; Blood Preservation ; Erythrocyte Transfusion ; filtration ; Humans ; leucocyte subsets ; leucodepletion ; Leukapheresis - methods ; Leukocytes - cytology ; red cells</subject><ispartof>Transfusion medicine (Oxford, England), 2000-03, Vol.10 (1), p.49-58</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4021-4b05ef84a90d78ef4c226b54d5b494aed52b9e958f39b49dc798dc4f5bd831283</citedby><cites>FETCH-LOGICAL-c4021-4b05ef84a90d78ef4c226b54d5b494aed52b9e958f39b49dc798dc4f5bd831283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-3148.2000.00231.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-3148.2000.00231.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10760203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rider, J R</creatorcontrib><creatorcontrib>Want, E J</creatorcontrib><creatorcontrib>Winter, M A</creatorcontrib><creatorcontrib>Turton, J R</creatorcontrib><creatorcontrib>Pamphilon, D H</creatorcontrib><creatorcontrib>Nobes, P</creatorcontrib><title>Differential leucocyte subpopulation analysis of leucodepleted red cell products</title><title>Transfusion medicine (Oxford, England)</title><addtitle>Transfusion Medicine</addtitle><description>Donor leucocytes are responsible for many adverse transfusion effects. Clinical reactions may be attributed to specific leucocyte subsets. In this study leucocyte subpopulations were identified and quantified pre‐ and post‐leucodepletion by integral filtration using novel Optipac® configurations incorporating either WBF‐1 (Pall Medsep) or RS2000 (Asahi) whole blood filters. Leucocytes were analysed by flow cytometry using direct, four‐colour, membrane immunofluorescence with monoclonal antibodies specific for CD 3, 14, 16, 19 and 45. Filtration reduced the leucocyte load by 3–4 log10, consistently giving products with < 2 cells μL−1. Subset distributions were also affected with the proportion of neutrophils and monocytes increased and the lymphocyte/monocyte ratio inverted. These effects were independent of the preprocessing hold conditions, filter used and buffy coat (BC) removal. All filtered red cell products contained 75–80% neutrophils, 16–20% monocytes and 2–7% lymphocytes. Results presented here demonstrate that whole blood filtration, and BC removal, significantly reduce the content and substantially alter the subpopulation distribution of the donor leucocytes remaining in leucodepleted red cell products.</description><subject>blood</subject><subject>Blood Preservation</subject><subject>Erythrocyte Transfusion</subject><subject>filtration</subject><subject>Humans</subject><subject>leucocyte subsets</subject><subject>leucodepletion</subject><subject>Leukapheresis - methods</subject><subject>Leukocytes - cytology</subject><subject>red cells</subject><issn>0958-7578</issn><issn>1365-3148</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1v1DAQhi1URJeWv4By4pYw_kociQvqxxYopVJb9Wg59ljK4t2kcaLu_nscUlUcOVi27OedGT-EZBQKCqL8vCkoL2XOqVAFA4ACgHFa7N-Q1evDEVlBLVVeyUodk_cxbgAoZzV7R44pVCUw4Ctye956jwPuxtaELOBkO3sYMYtT03f9FMzYdrvM7Ew4xDZmnV8Yh33AEV02pGUxhKwfOjfZMZ6St96EiB9e9hPycHlxf3aVX_9afzv7ep1bAYzmogGJXglTg6sUemEZKxspnGxELQw6yZoa0_ye1-nG2apWzgovG6c4ZYqfkE9L3dT4acI46m0b50nMDrsp6opC-qvkCVQLaIcuxgG97od2a4aDpqBnm3qjZ2l6lqZnm_qvTb1P0Y8vPaZmi-6f4KIvAV8W4LkNePjvwvr-50U6pHi-xNs44v41bobfuqx4JfXjzVrf3P34fr6-rXXJ_wDOaJNa</recordid><startdate>200003</startdate><enddate>200003</enddate><creator>Rider, J R</creator><creator>Want, E J</creator><creator>Winter, M A</creator><creator>Turton, J R</creator><creator>Pamphilon, D H</creator><creator>Nobes, P</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200003</creationdate><title>Differential leucocyte subpopulation analysis of leucodepleted red cell products</title><author>Rider, J R ; Want, E J ; Winter, M A ; Turton, J R ; Pamphilon, D H ; Nobes, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4021-4b05ef84a90d78ef4c226b54d5b494aed52b9e958f39b49dc798dc4f5bd831283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>blood</topic><topic>Blood Preservation</topic><topic>Erythrocyte Transfusion</topic><topic>filtration</topic><topic>Humans</topic><topic>leucocyte subsets</topic><topic>leucodepletion</topic><topic>Leukapheresis - methods</topic><topic>Leukocytes - cytology</topic><topic>red cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rider, J R</creatorcontrib><creatorcontrib>Want, E J</creatorcontrib><creatorcontrib>Winter, M A</creatorcontrib><creatorcontrib>Turton, J R</creatorcontrib><creatorcontrib>Pamphilon, D H</creatorcontrib><creatorcontrib>Nobes, P</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion medicine (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rider, J R</au><au>Want, E J</au><au>Winter, M A</au><au>Turton, J R</au><au>Pamphilon, D H</au><au>Nobes, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential leucocyte subpopulation analysis of leucodepleted red cell products</atitle><jtitle>Transfusion medicine (Oxford, England)</jtitle><addtitle>Transfusion Medicine</addtitle><date>2000-03</date><risdate>2000</risdate><volume>10</volume><issue>1</issue><spage>49</spage><epage>58</epage><pages>49-58</pages><issn>0958-7578</issn><eissn>1365-3148</eissn><abstract>Donor leucocytes are responsible for many adverse transfusion effects. Clinical reactions may be attributed to specific leucocyte subsets. In this study leucocyte subpopulations were identified and quantified pre‐ and post‐leucodepletion by integral filtration using novel Optipac® configurations incorporating either WBF‐1 (Pall Medsep) or RS2000 (Asahi) whole blood filters. Leucocytes were analysed by flow cytometry using direct, four‐colour, membrane immunofluorescence with monoclonal antibodies specific for CD 3, 14, 16, 19 and 45. Filtration reduced the leucocyte load by 3–4 log10, consistently giving products with < 2 cells μL−1. Subset distributions were also affected with the proportion of neutrophils and monocytes increased and the lymphocyte/monocyte ratio inverted. These effects were independent of the preprocessing hold conditions, filter used and buffy coat (BC) removal. All filtered red cell products contained 75–80% neutrophils, 16–20% monocytes and 2–7% lymphocytes. Results presented here demonstrate that whole blood filtration, and BC removal, significantly reduce the content and substantially alter the subpopulation distribution of the donor leucocytes remaining in leucodepleted red cell products.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>10760203</pmid><doi>10.1046/j.1365-3148.2000.00231.x</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0958-7578 |
| ispartof | Transfusion medicine (Oxford, England), 2000-03, Vol.10 (1), p.49-58 |
| issn | 0958-7578 1365-3148 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71029253 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | blood Blood Preservation Erythrocyte Transfusion filtration Humans leucocyte subsets leucodepletion Leukapheresis - methods Leukocytes - cytology red cells |
| title | Differential leucocyte subpopulation analysis of leucodepleted red cell products |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T20%3A48%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20leucocyte%20subpopulation%20analysis%20of%20leucodepleted%20red%20cell%20products&rft.jtitle=Transfusion%20medicine%20(Oxford,%20England)&rft.au=Rider,%20J%20R&rft.date=2000-03&rft.volume=10&rft.issue=1&rft.spage=49&rft.epage=58&rft.pages=49-58&rft.issn=0958-7578&rft.eissn=1365-3148&rft_id=info:doi/10.1046/j.1365-3148.2000.00231.x&rft_dat=%3Cproquest_cross%3E71029253%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71029253&rft_id=info:pmid/10760203&rfr_iscdi=true |