Function for Hedgehog Genes in Zebrafish Retinal Development
The hedgehog (hh) genes encode secreted signaling proteins that have important developmental functions in vertebrates and invertebrates. In Drosophila, expression of hh coordinates retinal development by propagating a wave of photoreceptor differentiation across the eye primordium. Here we report th...
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| Veröffentlicht in: | Developmental biology 2000-04, Vol.220 (2), p.238-252 |
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| creator | Stenkamp, Deborah L. Frey, Ruth A. Prabhudesai, Shubhangi N. Raymond, Pamela A. |
| description | The hedgehog (hh) genes encode secreted signaling proteins that have important developmental functions in vertebrates and invertebrates. In Drosophila, expression of hh coordinates retinal development by propagating a wave of photoreceptor differentiation across the eye primordium. Here we report that two vertebrate hh genes, sonic hedgehog (shh) and tiggy-winkle hedgehog (twhh), may perform similar functions in the developing zebrafish. Both shh and twhh are expressed in the embryonic zebrafish retinal pigmented epithelium (RPE), initially in a discrete ventral patch which then expands outward in advance of an expanding wave of photoreceptor recruitment in the subjacent neural retina. A gene encoding a receptor for the hedgehog protein, ptc-2, is expressed by retinal neuroepithelial cells. Injection of a cocktail of antisense (αshh/αtwhh) oligonucleotides reduces expression of both hh genes in the RPE and slows or arrests the progression of rod and cone photoreceptor differentiation. Zebrafish strains known to have mutations in Hh signaling pathway genes similarly exhibit retardation of photoreceptor differentiation. We propose that hedgehog genes may play a role in propagating photoreceptor differentiation across the developing eye of the zebrafish. |
| doi_str_mv | 10.1006/dbio.2000.9629 |
| format | Article |
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In Drosophila, expression of hh coordinates retinal development by propagating a wave of photoreceptor differentiation across the eye primordium. Here we report that two vertebrate hh genes, sonic hedgehog (shh) and tiggy-winkle hedgehog (twhh), may perform similar functions in the developing zebrafish. Both shh and twhh are expressed in the embryonic zebrafish retinal pigmented epithelium (RPE), initially in a discrete ventral patch which then expands outward in advance of an expanding wave of photoreceptor recruitment in the subjacent neural retina. A gene encoding a receptor for the hedgehog protein, ptc-2, is expressed by retinal neuroepithelial cells. Injection of a cocktail of antisense (αshh/αtwhh) oligonucleotides reduces expression of both hh genes in the RPE and slows or arrests the progression of rod and cone photoreceptor differentiation. Zebrafish strains known to have mutations in Hh signaling pathway genes similarly exhibit retardation of photoreceptor differentiation. 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We propose that hedgehog genes may play a role in propagating photoreceptor differentiation across the developing eye of the zebrafish.</description><subject>Animals</subject><subject>antisense oligonucleotide</subject><subject>Cell Differentiation</subject><subject>Danio rerio</subject><subject>Drosophila - embryology</subject><subject>Drosophila - genetics</subject><subject>Drosophila Proteins</subject><subject>Hedgehog gene</subject><subject>Hedgehog Proteins</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - physiology</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Proteins - physiology</subject><subject>Mutation</subject><subject>Oligonucleotides, Antisense - metabolism</subject><subject>opsin</subject><subject>photoreceptor</subject><subject>Photoreceptor Cells, Vertebrate - physiology</subject><subject>Proteins - metabolism</subject><subject>Proteins - physiology</subject><subject>ptc-2 gene</subject><subject>Retina - embryology</subject><subject>Retina - metabolism</subject><subject>retinal pigmented epithelium (RPE)</subject><subject>Retinal Rod Photoreceptor Cells - embryology</subject><subject>Retinal Rod Photoreceptor Cells - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rod Opsins - metabolism</subject><subject>sonic hedgehog gene</subject><subject>tiggy-winkle hedgehog gene</subject><subject>Trans-Activators</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish Proteins</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFLwzAUx_EgipvTq0fpyVvrS5umLXiR6aYwEERBvIQ2fdkibTKTduB_b8t28CKecvnkB-9LyCWFiALwm7rSNooBICp4XByRKYUiDVPO3o_JFIDGIeXAJ-TM-89BJXmenJIJhSxNUppMye2iN7LT1gTKuuAR6zVu7DpYokEfaBN8YOVKpf0meMFOm7IJ7nGHjd22aLpzcqLKxuPF4Z2Rt8XD6_wxXD0vn-Z3q1AySruwlDUreKZiiVTmPFE0r1IoOC1KqrhECRkwlaRpUmeqyhiLQTJVAasBCql4MiPX-92ts189-k602ktsmtKg7b3IKMR5PJz0H6RZFgPL2QCjPZTOeu9Qia3Tbem-BQUxhhVjWDGGFWPY4cPVYbmvWqx_8X3JAeR7gEOInUYnvNRoJNbaoexEbfVf2z-bRIYG</recordid><startdate>20000415</startdate><enddate>20000415</enddate><creator>Stenkamp, Deborah L.</creator><creator>Frey, Ruth A.</creator><creator>Prabhudesai, Shubhangi N.</creator><creator>Raymond, Pamela A.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000415</creationdate><title>Function for Hedgehog Genes in Zebrafish Retinal Development</title><author>Stenkamp, Deborah L. ; Frey, Ruth A. ; Prabhudesai, Shubhangi N. ; Raymond, Pamela A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-acd4967f2ce1c863f18b509619a1f6cec0704f3553d7fb74420c4fb04d009cf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>antisense oligonucleotide</topic><topic>Cell Differentiation</topic><topic>Danio rerio</topic><topic>Drosophila - embryology</topic><topic>Drosophila - genetics</topic><topic>Drosophila Proteins</topic><topic>Hedgehog gene</topic><topic>Hedgehog Proteins</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - physiology</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Proteins - physiology</topic><topic>Mutation</topic><topic>Oligonucleotides, Antisense - metabolism</topic><topic>opsin</topic><topic>photoreceptor</topic><topic>Photoreceptor Cells, Vertebrate - physiology</topic><topic>Proteins - metabolism</topic><topic>Proteins - physiology</topic><topic>ptc-2 gene</topic><topic>Retina - embryology</topic><topic>Retina - metabolism</topic><topic>retinal pigmented epithelium (RPE)</topic><topic>Retinal Rod Photoreceptor Cells - embryology</topic><topic>Retinal Rod Photoreceptor Cells - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rod Opsins - metabolism</topic><topic>sonic hedgehog gene</topic><topic>tiggy-winkle hedgehog gene</topic><topic>Trans-Activators</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stenkamp, Deborah L.</creatorcontrib><creatorcontrib>Frey, Ruth A.</creatorcontrib><creatorcontrib>Prabhudesai, Shubhangi N.</creatorcontrib><creatorcontrib>Raymond, Pamela A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stenkamp, Deborah L.</au><au>Frey, Ruth A.</au><au>Prabhudesai, Shubhangi N.</au><au>Raymond, Pamela A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Function for Hedgehog Genes in Zebrafish Retinal Development</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2000-04-15</date><risdate>2000</risdate><volume>220</volume><issue>2</issue><spage>238</spage><epage>252</epage><pages>238-252</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>The hedgehog (hh) genes encode secreted signaling proteins that have important developmental functions in vertebrates and invertebrates. In Drosophila, expression of hh coordinates retinal development by propagating a wave of photoreceptor differentiation across the eye primordium. Here we report that two vertebrate hh genes, sonic hedgehog (shh) and tiggy-winkle hedgehog (twhh), may perform similar functions in the developing zebrafish. Both shh and twhh are expressed in the embryonic zebrafish retinal pigmented epithelium (RPE), initially in a discrete ventral patch which then expands outward in advance of an expanding wave of photoreceptor recruitment in the subjacent neural retina. A gene encoding a receptor for the hedgehog protein, ptc-2, is expressed by retinal neuroepithelial cells. Injection of a cocktail of antisense (αshh/αtwhh) oligonucleotides reduces expression of both hh genes in the RPE and slows or arrests the progression of rod and cone photoreceptor differentiation. Zebrafish strains known to have mutations in Hh signaling pathway genes similarly exhibit retardation of photoreceptor differentiation. 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| subjects | Animals antisense oligonucleotide Cell Differentiation Danio rerio Drosophila - embryology Drosophila - genetics Drosophila Proteins Hedgehog gene Hedgehog Proteins Immunohistochemistry In Situ Hybridization Insect Proteins - genetics Insect Proteins - physiology Membrane Proteins - metabolism Membrane Proteins - physiology Mutation Oligonucleotides, Antisense - metabolism opsin photoreceptor Photoreceptor Cells, Vertebrate - physiology Proteins - metabolism Proteins - physiology ptc-2 gene Retina - embryology Retina - metabolism retinal pigmented epithelium (RPE) Retinal Rod Photoreceptor Cells - embryology Retinal Rod Photoreceptor Cells - metabolism Reverse Transcriptase Polymerase Chain Reaction Rod Opsins - metabolism sonic hedgehog gene tiggy-winkle hedgehog gene Trans-Activators Zebrafish - embryology Zebrafish - genetics Zebrafish Proteins |
| title | Function for Hedgehog Genes in Zebrafish Retinal Development |
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