Biodisposition of FITC-Labeled Aloemannan in Mice
Abstract Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine into smaller molecules that were effectively absorbed in mice. The homogenate from the other tissues did not affec...
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| Veröffentlicht in: | Planta medica 2001, Vol.67 (4), p.297-300 |
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| creator | Yagi, Akira Hamano, Shinya Tanaka, Tetsuro Kaneo, Yoshiharu Fujioka, Toshihiro Mihashi, Kunihide |
| description | Abstract
Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine into smaller molecules that were effectively absorbed in mice. The homogenate from the other tissues did not affect the metabolism of F-AM. The degraded product (1) of F-AM after incubation with 10 % feces homogenate for 24 h was chromatographed on a highly porous polymer and a Sephadex LH-20 column to provide an FITC-degraded fraction (2), which was shown to have a molecular weight of 800 D on Sephadex G-25 gel permeation. Metabolite 2 was examined by physicochemical methods and shown to be a mixture of FITC-hexose and -2 hexose on FAB-MS. An FITC-degraded fraction (3) with a molecular weight of 3 KD was obtained by 6-h incubation with 10 % feces homogenate on Sephadex G-25 column chromatography and was shown to be a mixture of FITC-9 and 12 × hexose on TOF-MS. |
| doi_str_mv | 10.1055/s-2001-14314 |
| format | Article |
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Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine into smaller molecules that were effectively absorbed in mice. The homogenate from the other tissues did not affect the metabolism of F-AM. The degraded product (1) of F-AM after incubation with 10 % feces homogenate for 24 h was chromatographed on a highly porous polymer and a Sephadex LH-20 column to provide an FITC-degraded fraction (2), which was shown to have a molecular weight of 800 D on Sephadex G-25 gel permeation. Metabolite 2 was examined by physicochemical methods and shown to be a mixture of FITC-hexose and -2 hexose on FAB-MS. An FITC-degraded fraction (3) with a molecular weight of 3 KD was obtained by 6-h incubation with 10 % feces homogenate on Sephadex G-25 column chromatography and was shown to be a mixture of FITC-9 and 12 × hexose on TOF-MS.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2001-14314</identifier><identifier>PMID: 11458442</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Adjuvants, Immunologic - pharmacokinetics ; Adjuvants, Immunologic - urine ; Aloe - chemistry ; Animals ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Feces - chemistry ; Fluorescein-5-isothiocyanate - chemistry ; General pharmacology ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Male ; Mannans - pharmacokinetics ; Mannans - urine ; Medical sciences ; Mice ; Mice, Inbred Strains ; Original Paper ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plants, Medicinal - chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>Planta medica, 2001, Vol.67 (4), p.297-300</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-65733c8757bbf73896199838d0e81254437bd6df90dc81008d15532761b2340d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2001-14314.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2001-14314$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3003,3004,4009,27902,27903,27904,54537,54538</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1044688$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11458442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yagi, Akira</creatorcontrib><creatorcontrib>Hamano, Shinya</creatorcontrib><creatorcontrib>Tanaka, Tetsuro</creatorcontrib><creatorcontrib>Kaneo, Yoshiharu</creatorcontrib><creatorcontrib>Fujioka, Toshihiro</creatorcontrib><creatorcontrib>Mihashi, Kunihide</creatorcontrib><title>Biodisposition of FITC-Labeled Aloemannan in Mice</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine into smaller molecules that were effectively absorbed in mice. The homogenate from the other tissues did not affect the metabolism of F-AM. The degraded product (1) of F-AM after incubation with 10 % feces homogenate for 24 h was chromatographed on a highly porous polymer and a Sephadex LH-20 column to provide an FITC-degraded fraction (2), which was shown to have a molecular weight of 800 D on Sephadex G-25 gel permeation. Metabolite 2 was examined by physicochemical methods and shown to be a mixture of FITC-hexose and -2 hexose on FAB-MS. An FITC-degraded fraction (3) with a molecular weight of 3 KD was obtained by 6-h incubation with 10 % feces homogenate on Sephadex G-25 column chromatography and was shown to be a mixture of FITC-9 and 12 × hexose on TOF-MS.</description><subject>Adjuvants, Immunologic - pharmacokinetics</subject><subject>Adjuvants, Immunologic - urine</subject><subject>Aloe - chemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Feces - chemistry</subject><subject>Fluorescein-5-isothiocyanate - chemistry</subject><subject>General pharmacology</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Male</subject><subject>Mannans - pharmacokinetics</subject><subject>Mannans - urine</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Original Paper</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plants, Medicinal - chemistry</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0D9PwzAQhnELgWgpbMwoA2KBwJ3_JM5YKgqViljKbDmxI1wlcYmTgW9PSiPBwHTLT-9JDyGXCPcIQjyEmAJgjJwhPyLT4WYxUIrHZArAaAwZZxNyFsJ2YDwDOCUTRC4k53RK8NF548LOB9c530S-jJarzSJe69xW1kTzyttaN41uItdEr66w5-Sk1FWwF-Odkffl02bxEq_fnleL-TouOMouTkTKWCFTkeZ5mTKZJZhlkkkDViIVnLM0N4kpMzCFRABpUAhG0wRzyjgYNiM3h91d6z97GzpVu1DYqtKN9X1QKQIVVGYDvDvAovUhtLZUu9bVuv1SCGqfSAW1T6R-Eg38atzt89qaXzw2GcD1CHQodFW2uilc-DPKeSLlwG4PrPtwtrZq6_u2GYL8__Ubg_l3QA</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Yagi, Akira</creator><creator>Hamano, Shinya</creator><creator>Tanaka, Tetsuro</creator><creator>Kaneo, Yoshiharu</creator><creator>Fujioka, Toshihiro</creator><creator>Mihashi, Kunihide</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>Biodisposition of FITC-Labeled Aloemannan in Mice</title><author>Yagi, Akira ; Hamano, Shinya ; Tanaka, Tetsuro ; Kaneo, Yoshiharu ; Fujioka, Toshihiro ; Mihashi, Kunihide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-65733c8757bbf73896199838d0e81254437bd6df90dc81008d15532761b2340d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adjuvants, Immunologic - pharmacokinetics</topic><topic>Adjuvants, Immunologic - urine</topic><topic>Aloe - chemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Feces - chemistry</topic><topic>Fluorescein-5-isothiocyanate - chemistry</topic><topic>General pharmacology</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Male</topic><topic>Mannans - pharmacokinetics</topic><topic>Mannans - urine</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Original Paper</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plants, Medicinal - chemistry</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yagi, Akira</creatorcontrib><creatorcontrib>Hamano, Shinya</creatorcontrib><creatorcontrib>Tanaka, Tetsuro</creatorcontrib><creatorcontrib>Kaneo, Yoshiharu</creatorcontrib><creatorcontrib>Fujioka, Toshihiro</creatorcontrib><creatorcontrib>Mihashi, Kunihide</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yagi, Akira</au><au>Hamano, Shinya</au><au>Tanaka, Tetsuro</au><au>Kaneo, Yoshiharu</au><au>Fujioka, Toshihiro</au><au>Mihashi, Kunihide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biodisposition of FITC-Labeled Aloemannan in Mice</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2001</date><risdate>2001</risdate><volume>67</volume><issue>4</issue><spage>297</spage><epage>300</epage><pages>297-300</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine into smaller molecules that were effectively absorbed in mice. The homogenate from the other tissues did not affect the metabolism of F-AM. The degraded product (1) of F-AM after incubation with 10 % feces homogenate for 24 h was chromatographed on a highly porous polymer and a Sephadex LH-20 column to provide an FITC-degraded fraction (2), which was shown to have a molecular weight of 800 D on Sephadex G-25 gel permeation. Metabolite 2 was examined by physicochemical methods and shown to be a mixture of FITC-hexose and -2 hexose on FAB-MS. An FITC-degraded fraction (3) with a molecular weight of 3 KD was obtained by 6-h incubation with 10 % feces homogenate on Sephadex G-25 column chromatography and was shown to be a mixture of FITC-9 and 12 × hexose on TOF-MS.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>11458442</pmid><doi>10.1055/s-2001-14314</doi><tpages>4</tpages></addata></record> |
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| subjects | Adjuvants, Immunologic - pharmacokinetics Adjuvants, Immunologic - urine Aloe - chemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid Feces - chemistry Fluorescein-5-isothiocyanate - chemistry General pharmacology Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Male Mannans - pharmacokinetics Mannans - urine Medical sciences Mice Mice, Inbred Strains Original Paper Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plants, Medicinal - chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Biodisposition of FITC-Labeled Aloemannan in Mice |
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