Authentication of Chinese Crude Drug, Gecko, by Allele-Specific Diagnostic PCR

Abstract Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The re...

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Veröffentlicht in:	Planta medica 2001, Vol.67 (4), p.385-387
Hauptverfasser:	Liu, Zhongquan, Wang, Yiquan, Zhou, Kaiya, Han, Demin, Yang, Xuegan, Liu, Xunhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Electrophoresis, Agar Gel General pharmacology Letter Lizards Medical sciences Medicine, Chinese Traditional Mitochondria - genetics Molecular Sequence Data Pharmaceutical Preparations - analysis Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Polymerase Chain Reaction - methods RNA, Ribosomal - analysis Salamandridae Sequence Analysis, DNA
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