Authentication of Chinese Crude Drug, Gecko, by Allele-Specific Diagnostic PCR
Abstract Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The re...
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| Veröffentlicht in: | Planta medica 2001, Vol.67 (4), p.385-387 |
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| container_title | Planta medica |
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| creator | Liu, Zhongquan Wang, Yiquan Zhou, Kaiya Han, Demin Yang, Xuegan Liu, Xunhong |
| description | Abstract
Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The results of amplification with the primers indicate that amplicons from the templates of GEKKO GECKO were clearly revealed by agarose gel electrophoresis, whereas no evident amplicons were found from other species. The primers were employed to identify crude drug samples from different sources. Among a total of 9 samples, 3 were diagnosed as genuine Gecko. This result is consistent with morphological identification and DNA sequence analyses. |
| doi_str_mv | 10.1055/s-2001-14321 |
| format | Article |
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Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The results of amplification with the primers indicate that amplicons from the templates of GEKKO GECKO were clearly revealed by agarose gel electrophoresis, whereas no evident amplicons were found from other species. The primers were employed to identify crude drug samples from different sources. Among a total of 9 samples, 3 were diagnosed as genuine Gecko. This result is consistent with morphological identification and DNA sequence analyses.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2001-14321</identifier><identifier>PMID: 11458468</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Electrophoresis, Agar Gel ; General pharmacology ; Letter ; Lizards ; Medical sciences ; Medicine, Chinese Traditional ; Mitochondria - genetics ; Molecular Sequence Data ; Pharmaceutical Preparations - analysis ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Polymerase Chain Reaction - methods ; RNA, Ribosomal - analysis ; Salamandridae ; Sequence Analysis, DNA</subject><ispartof>Planta medica, 2001, Vol.67 (4), p.385-387</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-5e92eb672abe1b397d0edb054b9c4439a4958e6c03fd14700533769d5d8265e53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2001-14321.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2001-14321$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3004,3005,4010,27900,27901,27902,54534,54535</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1046062$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11458468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Zhongquan</creatorcontrib><creatorcontrib>Wang, Yiquan</creatorcontrib><creatorcontrib>Zhou, Kaiya</creatorcontrib><creatorcontrib>Han, Demin</creatorcontrib><creatorcontrib>Yang, Xuegan</creatorcontrib><creatorcontrib>Liu, Xunhong</creatorcontrib><title>Authentication of Chinese Crude Drug, Gecko, by Allele-Specific Diagnostic PCR</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The results of amplification with the primers indicate that amplicons from the templates of GEKKO GECKO were clearly revealed by agarose gel electrophoresis, whereas no evident amplicons were found from other species. The primers were employed to identify crude drug samples from different sources. Among a total of 9 samples, 3 were diagnosed as genuine Gecko. This result is consistent with morphological identification and DNA sequence analyses.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Agar Gel</subject><subject>General pharmacology</subject><subject>Letter</subject><subject>Lizards</subject><subject>Medical sciences</subject><subject>Medicine, Chinese Traditional</subject><subject>Mitochondria - genetics</subject><subject>Molecular Sequence Data</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA, Ribosomal - analysis</subject><subject>Salamandridae</subject><subject>Sequence Analysis, DNA</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkDtPwzAURi0EouWxMSMPiIUGrh07j7EKTwkB4jFbjnPTGtKk2MnAv8ellWBgusvR-XQPIUcMzhlIeeEjDsAiJmLOtsg43DwCztk2GQPEPIJcxCOy5_17wEQOsEtGjAmZiSQbk4fp0M-x7a3Rve1a2tW0mNsWPdLCDRXSSzfMJvQGzUc3oeUXnTYNNhi9LNHY2hp6afWs7XwQ0Kfi-YDs1LrxeLi5--Tt-uq1uI3uH2_uiul9ZATL-khizrFMUq5LZGWcpxVgVYIUZW5EeECLXGaYGIjriokUQMZxmuSVrDKeSJTxPjlde5eu-xzQ92phvcGm0S12g1cpAy4SWIGTNWhc573DWi2dXWj3pRioVT_l1aqf-ukX8OONdygXWP3Cm2ABONkA2hvd1E63xvo_0rCa8ICdrbF-bnGB6r0bXBuC_L_6Df4Pgnw</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Liu, Zhongquan</creator><creator>Wang, Yiquan</creator><creator>Zhou, Kaiya</creator><creator>Han, Demin</creator><creator>Yang, Xuegan</creator><creator>Liu, Xunhong</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>Authentication of Chinese Crude Drug, Gecko, by Allele-Specific Diagnostic PCR</title><author>Liu, Zhongquan ; Wang, Yiquan ; Zhou, Kaiya ; Han, Demin ; Yang, Xuegan ; Liu, Xunhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-5e92eb672abe1b397d0edb054b9c4439a4958e6c03fd14700533769d5d8265e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Agar Gel</topic><topic>General pharmacology</topic><topic>Letter</topic><topic>Lizards</topic><topic>Medical sciences</topic><topic>Medicine, Chinese Traditional</topic><topic>Mitochondria - genetics</topic><topic>Molecular Sequence Data</topic><topic>Pharmaceutical Preparations - analysis</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA, Ribosomal - analysis</topic><topic>Salamandridae</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Zhongquan</creatorcontrib><creatorcontrib>Wang, Yiquan</creatorcontrib><creatorcontrib>Zhou, Kaiya</creatorcontrib><creatorcontrib>Han, Demin</creatorcontrib><creatorcontrib>Yang, Xuegan</creatorcontrib><creatorcontrib>Liu, Xunhong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Zhongquan</au><au>Wang, Yiquan</au><au>Zhou, Kaiya</au><au>Han, Demin</au><au>Yang, Xuegan</au><au>Liu, Xunhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Authentication of Chinese Crude Drug, Gecko, by Allele-Specific Diagnostic PCR</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2001</date><risdate>2001</risdate><volume>67</volume><issue>4</issue><spage>385</spage><epage>387</epage><pages>385-387</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The results of amplification with the primers indicate that amplicons from the templates of GEKKO GECKO were clearly revealed by agarose gel electrophoresis, whereas no evident amplicons were found from other species. The primers were employed to identify crude drug samples from different sources. Among a total of 9 samples, 3 were diagnosed as genuine Gecko. This result is consistent with morphological identification and DNA sequence analyses.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>11458468</pmid><doi>10.1055/s-2001-14321</doi><tpages>3</tpages></addata></record> |
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| ispartof | Planta medica, 2001, Vol.67 (4), p.385-387 |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | Animals Base Sequence Biological and medical sciences Electrophoresis, Agar Gel General pharmacology Letter Lizards Medical sciences Medicine, Chinese Traditional Mitochondria - genetics Molecular Sequence Data Pharmaceutical Preparations - analysis Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Polymerase Chain Reaction - methods RNA, Ribosomal - analysis Salamandridae Sequence Analysis, DNA |
| title | Authentication of Chinese Crude Drug, Gecko, by Allele-Specific Diagnostic PCR |
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