Interesterification (Acidolysis) of Butterfat with Conjugated Linoleic Acid in a Batch Reactor
Six commercial lipases, in free or immobilized form, were tested for their ability to catalyze acyl exchange between conjugated linoleic acid and anhydrous butterfat under solvent-free conditions. Immobilized Candida antarctica lipase exhibited the best activity. Experiments were conducted for this...
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| Veröffentlicht in: | Journal of dairy science 2000-03, Vol.83 (3), p.371-377 |
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| creator | Garcia, H.S. Keough, K.J. Arcos, J.A. Hill, C.G. |
| description | Six commercial lipases, in free or immobilized form, were tested for their ability to catalyze acyl exchange between conjugated linoleic acid and anhydrous butterfat under solvent-free conditions. Immobilized Candida antarctica lipase exhibited the best activity. Experiments were conducted for this lipase in butterfat to conjugated linoleic acid ratios of 10:1 (vol/vol), temperatures from 30 to 70°C, enzyme concentrations of 50 to 200 mg/g of reaction mixture, and water contents of 0.15 to 2% (wt/wt). At the maximum enzyme concentration used, equilibrium was reached within the first 24h of reaction. The optimum temperature was 50°C. The triacylglycerol profile of the product butterfat reflected changes in the relative proportions of fatty acid residues as the reaction proceeded, with increases in those triacylglycerols containing 46 to 54 carbon atoms and concomitant decreases in those triacylglycerols containing 34 to 42 carbon atoms. |
| doi_str_mv | 10.3168/jds.S0022-0302(00)74891-0 |
| format | Article |
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Immobilized Candida antarctica lipase exhibited the best activity. Experiments were conducted for this lipase in butterfat to conjugated linoleic acid ratios of 10:1 (vol/vol), temperatures from 30 to 70°C, enzyme concentrations of 50 to 200 mg/g of reaction mixture, and water contents of 0.15 to 2% (wt/wt). At the maximum enzyme concentration used, equilibrium was reached within the first 24h of reaction. The optimum temperature was 50°C. The triacylglycerol profile of the product butterfat reflected changes in the relative proportions of fatty acid residues as the reaction proceeded, with increases in those triacylglycerols containing 46 to 54 carbon atoms and concomitant decreases in those triacylglycerols containing 34 to 42 carbon atoms.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(00)74891-0</identifier><identifier>PMID: 10750090</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Elsevier Inc</publisher><subject>acidolysis ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Butter - analysis ; butterfat ; Candida - enzymology ; Chromatography, Gas ; Chromatography, High Pressure Liquid ; conjugated linoleic acid ; Esterification ; Fat industries ; Fats - metabolism ; Food industries ; Fundamental and applied biological sciences. Psychology ; interesterification ; Kinetics ; Linoleic Acid - metabolism ; Lipase - metabolism ; Methods. Procedures. Technologies ; Temperature ; Triglycerides - analysis ; Water</subject><ispartof>Journal of dairy science, 2000-03, Vol.83 (3), p.371-377</ispartof><rights>2000 American Dairy Science Association</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-2ec2c1e1ac8451d2c906bba39e333d4a49a4401764ff30d14462922c46818a543</citedby><cites>FETCH-LOGICAL-c511t-2ec2c1e1ac8451d2c906bba39e333d4a49a4401764ff30d14462922c46818a543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.S0022-0302(00)74891-0$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27869,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1315410$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10750090$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia, H.S.</creatorcontrib><creatorcontrib>Keough, K.J.</creatorcontrib><creatorcontrib>Arcos, J.A.</creatorcontrib><creatorcontrib>Hill, C.G.</creatorcontrib><title>Interesterification (Acidolysis) of Butterfat with Conjugated Linoleic Acid in a Batch Reactor</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>Six commercial lipases, in free or immobilized form, were tested for their ability to catalyze acyl exchange between conjugated linoleic acid and anhydrous butterfat under solvent-free conditions. Immobilized Candida antarctica lipase exhibited the best activity. Experiments were conducted for this lipase in butterfat to conjugated linoleic acid ratios of 10:1 (vol/vol), temperatures from 30 to 70°C, enzyme concentrations of 50 to 200 mg/g of reaction mixture, and water contents of 0.15 to 2% (wt/wt). At the maximum enzyme concentration used, equilibrium was reached within the first 24h of reaction. The optimum temperature was 50°C. The triacylglycerol profile of the product butterfat reflected changes in the relative proportions of fatty acid residues as the reaction proceeded, with increases in those triacylglycerols containing 46 to 54 carbon atoms and concomitant decreases in those triacylglycerols containing 34 to 42 carbon atoms.</description><subject>acidolysis</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Butter - analysis</subject><subject>butterfat</subject><subject>Candida - enzymology</subject><subject>Chromatography, Gas</subject><subject>Chromatography, High Pressure Liquid</subject><subject>conjugated linoleic acid</subject><subject>Esterification</subject><subject>Fat industries</subject><subject>Fats - metabolism</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>interesterification</subject><subject>Kinetics</subject><subject>Linoleic Acid - metabolism</subject><subject>Lipase - metabolism</subject><subject>Methods. Procedures. 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Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Butter - analysis</topic><topic>butterfat</topic><topic>Candida - enzymology</topic><topic>Chromatography, Gas</topic><topic>Chromatography, High Pressure Liquid</topic><topic>conjugated linoleic acid</topic><topic>Esterification</topic><topic>Fat industries</topic><topic>Fats - metabolism</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>interesterification</topic><topic>Kinetics</topic><topic>Linoleic Acid - metabolism</topic><topic>Lipase - metabolism</topic><topic>Methods. Procedures. 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Immobilized Candida antarctica lipase exhibited the best activity. Experiments were conducted for this lipase in butterfat to conjugated linoleic acid ratios of 10:1 (vol/vol), temperatures from 30 to 70°C, enzyme concentrations of 50 to 200 mg/g of reaction mixture, and water contents of 0.15 to 2% (wt/wt). At the maximum enzyme concentration used, equilibrium was reached within the first 24h of reaction. The optimum temperature was 50°C. The triacylglycerol profile of the product butterfat reflected changes in the relative proportions of fatty acid residues as the reaction proceeded, with increases in those triacylglycerols containing 46 to 54 carbon atoms and concomitant decreases in those triacylglycerols containing 34 to 42 carbon atoms.</abstract><cop>Savoy, IL</cop><pub>Elsevier Inc</pub><pmid>10750090</pmid><doi>10.3168/jds.S0022-0302(00)74891-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of dairy science, 2000-03, Vol.83 (3), p.371-377 |
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| subjects | acidolysis Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Butter - analysis butterfat Candida - enzymology Chromatography, Gas Chromatography, High Pressure Liquid conjugated linoleic acid Esterification Fat industries Fats - metabolism Food industries Fundamental and applied biological sciences. Psychology interesterification Kinetics Linoleic Acid - metabolism Lipase - metabolism Methods. Procedures. Technologies Temperature Triglycerides - analysis Water |
| title | Interesterification (Acidolysis) of Butterfat with Conjugated Linoleic Acid in a Batch Reactor |
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