Enzymatic synthesis of oligosaccharides by two glycosyl hydrolases of Sulfolobus solfataricus

The importance of carbohydrates in a variety of biological functions is the reason that interest has recently increased in these compounds as possible components of therapeutic agents. Thus, the need for a technique allowing the easy synthesis of carbohydrates and glucoconjugates is an emerging chal...

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Veröffentlicht in:Extremophiles (Tokyo. Print) 2001-06, Vol.5 (3), p.145-152
Hauptverfasser: MORACCI, Marco, TRINCONE, Antonio, COBUCCI-PONZANO, Beatrice, PERUGINO, Giuseppe, CIARAMELLA, Maria, ROSSI, Mosè
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container_issue 3
container_start_page 145
container_title Extremophiles (Tokyo. Print)
container_volume 5
creator MORACCI, Marco
TRINCONE, Antonio
COBUCCI-PONZANO, Beatrice
PERUGINO, Giuseppe
CIARAMELLA, Maria
ROSSI, Mosè
description The importance of carbohydrates in a variety of biological functions is the reason that interest has recently increased in these compounds as possible components of therapeutic agents. Thus, the need for a technique allowing the easy synthesis of carbohydrates and glucoconjugates is an emerging challenge for chemists and biologists involved in this field. At present, enzymatic synthesis has resulted in the most promising approach for the production of complex oligosaccharides. In this respect, the enzymological characteristics of the catalysts, in term of regioselectivity, substrate specificity, and operational stability, are of fundamental importance to improve the yields of the process and to widen the repertoire of the available products. Here, two methods of oligosaccharide synthesis performed by a glycosynthase and by an alpha-xylosidase from the hyperthermophilic archaeon Sulfolobus solfataricus are briefly reviewed. The approaches used and the biodiversity of the catalysts together are key features for their possible utilization in the synthesis of oligosaccharides.
doi_str_mv 10.1007/s007920100186
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subjects Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Carbohydrate Conformation
Carbohydrate Sequence
Catalysis
Enzyme Stability
Fundamental and applied biological sciences. Psychology
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Kinetics
Methods. Procedures. Technologies
Molecular Sequence Data
Mutation
Oligosaccharides - biosynthesis
Oligosaccharides - chemistry
Space life sciences
Sulfolobus - enzymology
Sulfolobus - genetics
Temperature
Xylosidases - metabolism
title Enzymatic synthesis of oligosaccharides by two glycosyl hydrolases of Sulfolobus solfataricus
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