Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles
Gap junctions allow direct intercellular coupling between many cells including those in the vascular wall. Studies of connexin expression in cells of the microcirculatory system are very few in number. However, cell-to-cell communication between cells of the arteriolar wall may be particularly impor...
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| Veröffentlicht in: | Histochemistry and cell biology 2001-06, Vol.115 (6), p.479-487 |
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| creator | Arensbak, B Mikkelsen, H B Gustafsson, F Christensen, T Holstein-Rathlou, N H |
| description | Gap junctions allow direct intercellular coupling between many cells including those in the vascular wall. Studies of connexin expression in cells of the microcirculatory system are very few in number. However, cell-to-cell communication between cells of the arteriolar wall may be particularly important in microcirculatory control. We investigated the expression of connexins 43, 40, and 37 (Cx43, Cx40, Cx37) mRNA and proteins in primary cultures of smooth muscle cells (SMC) from rat renal preglomerular arterioles and in the aortic cell line A7r5. Furthermore protein expression in preglomerular arterioles in frozen sections was evaluated. SMC were isolated from kidneys using an iron oxide sieve method and explant technique. Total RNA from these cultures was tested by RT-PCR analysis for the expression of the three connexins mRNA. Using immunofluorescence we examined whether the expression pattern of connexin protein in the cell culture and frozen sections corresponded to the mRNA expression. The data show that A7r5 and preglomerular SMC express mRNA for Cx37 in addition to Cx43 and Cx40. In A7r5 cells the mRNA for Cx43, Cx40, and Cx37 are translated to protein, whereas cultured preglomerular SMC and the media of afferent arterioles in frozen sections only showed Cx40 immunoreactivity. |
| doi_str_mv | 10.1007/s004180100275 |
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Studies of connexin expression in cells of the microcirculatory system are very few in number. However, cell-to-cell communication between cells of the arteriolar wall may be particularly important in microcirculatory control. We investigated the expression of connexins 43, 40, and 37 (Cx43, Cx40, Cx37) mRNA and proteins in primary cultures of smooth muscle cells (SMC) from rat renal preglomerular arterioles and in the aortic cell line A7r5. Furthermore protein expression in preglomerular arterioles in frozen sections was evaluated. SMC were isolated from kidneys using an iron oxide sieve method and explant technique. Total RNA from these cultures was tested by RT-PCR analysis for the expression of the three connexins mRNA. Using immunofluorescence we examined whether the expression pattern of connexin protein in the cell culture and frozen sections corresponded to the mRNA expression. The data show that A7r5 and preglomerular SMC express mRNA for Cx37 in addition to Cx43 and Cx40. In A7r5 cells the mRNA for Cx43, Cx40, and Cx37 are translated to protein, whereas cultured preglomerular SMC and the media of afferent arterioles in frozen sections only showed Cx40 immunoreactivity.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s004180100275</identifier><identifier>PMID: 11455448</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Aorta ; Arterioles ; Arterioles - cytology ; Arterioles - metabolism ; Cell culture ; Cell interactions ; Cells, Cultured ; Connexin 43 ; Connexin 43 - biosynthesis ; Connexin 43 - genetics ; Connexins - biosynthesis ; Connexins - genetics ; Gap Junction alpha-4 Protein ; Gap Junction alpha-5 Protein ; Gap junctions ; Gene Expression ; Humans ; Immunofluorescence ; Immunoreactivity ; Iron oxides ; Kidney Glomerulus - blood supply ; Kidneys ; Male ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - metabolism ; Polymerase chain reaction ; Proteins ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; RNA, Messenger ; Sensory neurons ; Smooth muscle</subject><ispartof>Histochemistry and cell biology, 2001-06, Vol.115 (6), p.479-487</ispartof><rights>Springer-Verlag 2001.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-e741c416fb001f00fcaf3e1a8925d68f9465479d8d0ffd9abc8895977ebe6a853</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11455448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arensbak, B</creatorcontrib><creatorcontrib>Mikkelsen, H B</creatorcontrib><creatorcontrib>Gustafsson, F</creatorcontrib><creatorcontrib>Christensen, T</creatorcontrib><creatorcontrib>Holstein-Rathlou, N H</creatorcontrib><title>Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><description>Gap junctions allow direct intercellular coupling between many cells including those in the vascular wall. Studies of connexin expression in cells of the microcirculatory system are very few in number. However, cell-to-cell communication between cells of the arteriolar wall may be particularly important in microcirculatory control. We investigated the expression of connexins 43, 40, and 37 (Cx43, Cx40, Cx37) mRNA and proteins in primary cultures of smooth muscle cells (SMC) from rat renal preglomerular arterioles and in the aortic cell line A7r5. Furthermore protein expression in preglomerular arterioles in frozen sections was evaluated. SMC were isolated from kidneys using an iron oxide sieve method and explant technique. Total RNA from these cultures was tested by RT-PCR analysis for the expression of the three connexins mRNA. Using immunofluorescence we examined whether the expression pattern of connexin protein in the cell culture and frozen sections corresponded to the mRNA expression. The data show that A7r5 and preglomerular SMC express mRNA for Cx37 in addition to Cx43 and Cx40. In A7r5 cells the mRNA for Cx43, Cx40, and Cx37 are translated to protein, whereas cultured preglomerular SMC and the media of afferent arterioles in frozen sections only showed Cx40 immunoreactivity.</description><subject>Animals</subject><subject>Aorta</subject><subject>Arterioles</subject><subject>Arterioles - cytology</subject><subject>Arterioles - metabolism</subject><subject>Cell culture</subject><subject>Cell interactions</subject><subject>Cells, Cultured</subject><subject>Connexin 43</subject><subject>Connexin 43 - biosynthesis</subject><subject>Connexin 43 - genetics</subject><subject>Connexins - biosynthesis</subject><subject>Connexins - genetics</subject><subject>Gap Junction alpha-4 Protein</subject><subject>Gap Junction alpha-5 Protein</subject><subject>Gap junctions</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Immunoreactivity</subject><subject>Iron oxides</subject><subject>Kidney Glomerulus - blood supply</subject><subject>Kidneys</subject><subject>Male</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger</subject><subject>Sensory neurons</subject><subject>Smooth muscle</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkN9LwzAQx4Mobk4ffZWC4NOqlyZpkkcZ8wcMBVHYW0nbi3S0zUxWmP-90Q1E4eC-3H34cvcl5JzCNQWQNwGAUwVRZ1IckDHlLEsp1ctDMgbNVZrHyYichLACoEJn2TEZUcqF4FyNyXK-XXsMoXF94mxSub7HbdMnTE4TDtPE9HXCWdK9PN3-6LV3G4z7WB5708YBvreuQz-0xifGb9A3rsVwSo6saQOe7fuEvN3NX2cP6eL5_nF2u0grptgmRclpxWluy3idBbCVsQypUToTda6s5rngUteqBmtrbcpKKS20lFhibpRgE3K1842XfQwYNkXXhArb1vTohlBIClRLnUXw8h-4coOPL4Qi4yAFZMB4pNIdVXkXgkdbrH3TGf9ZUCi-Ay_-BB75i73rUHZY_9L7hNkXywh4rQ</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Arensbak, B</creator><creator>Mikkelsen, H B</creator><creator>Gustafsson, F</creator><creator>Christensen, T</creator><creator>Holstein-Rathlou, N H</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles</title><author>Arensbak, B ; Mikkelsen, H B ; Gustafsson, F ; Christensen, T ; Holstein-Rathlou, N H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-e741c416fb001f00fcaf3e1a8925d68f9465479d8d0ffd9abc8895977ebe6a853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Aorta</topic><topic>Arterioles</topic><topic>Arterioles - cytology</topic><topic>Arterioles - metabolism</topic><topic>Cell culture</topic><topic>Cell interactions</topic><topic>Cells, Cultured</topic><topic>Connexin 43</topic><topic>Connexin 43 - biosynthesis</topic><topic>Connexin 43 - genetics</topic><topic>Connexins - biosynthesis</topic><topic>Connexins - genetics</topic><topic>Gap Junction alpha-4 Protein</topic><topic>Gap Junction alpha-5 Protein</topic><topic>Gap junctions</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Immunoreactivity</topic><topic>Iron oxides</topic><topic>Kidney Glomerulus - blood supply</topic><topic>Kidneys</topic><topic>Male</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger</topic><topic>Sensory neurons</topic><topic>Smooth muscle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arensbak, B</creatorcontrib><creatorcontrib>Mikkelsen, H B</creatorcontrib><creatorcontrib>Gustafsson, F</creatorcontrib><creatorcontrib>Christensen, T</creatorcontrib><creatorcontrib>Holstein-Rathlou, N H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arensbak, B</au><au>Mikkelsen, H B</au><au>Gustafsson, F</au><au>Christensen, T</au><au>Holstein-Rathlou, N H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles</atitle><jtitle>Histochemistry and cell biology</jtitle><addtitle>Histochem Cell Biol</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>115</volume><issue>6</issue><spage>479</spage><epage>487</epage><pages>479-487</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>Gap junctions allow direct intercellular coupling between many cells including those in the vascular wall. Studies of connexin expression in cells of the microcirculatory system are very few in number. However, cell-to-cell communication between cells of the arteriolar wall may be particularly important in microcirculatory control. We investigated the expression of connexins 43, 40, and 37 (Cx43, Cx40, Cx37) mRNA and proteins in primary cultures of smooth muscle cells (SMC) from rat renal preglomerular arterioles and in the aortic cell line A7r5. Furthermore protein expression in preglomerular arterioles in frozen sections was evaluated. SMC were isolated from kidneys using an iron oxide sieve method and explant technique. Total RNA from these cultures was tested by RT-PCR analysis for the expression of the three connexins mRNA. Using immunofluorescence we examined whether the expression pattern of connexin protein in the cell culture and frozen sections corresponded to the mRNA expression. The data show that A7r5 and preglomerular SMC express mRNA for Cx37 in addition to Cx43 and Cx40. In A7r5 cells the mRNA for Cx43, Cx40, and Cx37 are translated to protein, whereas cultured preglomerular SMC and the media of afferent arterioles in frozen sections only showed Cx40 immunoreactivity.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>11455448</pmid><doi>10.1007/s004180100275</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Aorta Arterioles Arterioles - cytology Arterioles - metabolism Cell culture Cell interactions Cells, Cultured Connexin 43 Connexin 43 - biosynthesis Connexin 43 - genetics Connexins - biosynthesis Connexins - genetics Gap Junction alpha-4 Protein Gap Junction alpha-5 Protein Gap junctions Gene Expression Humans Immunofluorescence Immunoreactivity Iron oxides Kidney Glomerulus - blood supply Kidneys Male Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - metabolism Polymerase chain reaction Proteins Rats Rats, Sprague-Dawley Rats, Wistar RNA, Messenger Sensory neurons Smooth muscle |
| title | Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles |
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