Developmental regulation of AMPA-receptor properties in CA1 pyramidal neurons of rat hippocampus
AMPA-receptor (AMPA-R) currents were recorded from CA1 pyramidal neurons in situ and after acute isolation from the hippocampus of 3- to 45-day-old rats. Membrane currents were analyzed by combining the patch clamp method with fast application techniques. The complete block of receptor currents by G...
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| Veröffentlicht in: | Neuropharmacology 2000-01, Vol.39 (6), p.931-942 |
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| creator | Seifert, Gerald Zhou, Min Dietrich, Dirk Schumacher, Thekla B. Dybek, Andre Weiser, Thomas Wienrich, Marion Wilhelm, Doris Steinhäuser, Christian |
| description | AMPA-receptor (AMPA-R) currents were recorded from CA1 pyramidal neurons in situ and after acute isolation from the hippocampus of 3- to 45-day-old rats. Membrane currents were analyzed by combining the patch clamp method with fast application techniques. The complete block of receptor currents by GYKI 53655 and the absence of modulation by Concanavalin A indicated that the cells exclusively expressed non-NMDA glutamate receptors of the AMPA subtype while functional kainate receptors could not be detected. The lowest sensitivity to kainate and NBQX was observed at postnatal day (p) 18. These changes might reflect a lower abundance of GluR1 at that developmental stage. A decrease of potentiation of receptor currents by cyclothiazide (CTZ), an acceleration of the recovery from CTZ potentiation and a faster and more complete desensitization of glutamate-evoked currents suggest an up-regulation of flop splice variants with increasing age. These functional data indicate that AMPA-R expression in CA1 pyramidal neurons varies during postnatal development which can be expected to influence the kinetics of synaptic transmission and the excitotoxic vulnerability as well. |
| doi_str_mv | 10.1016/S0028-3908(99)00212-9 |
| format | Article |
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Membrane currents were analyzed by combining the patch clamp method with fast application techniques. The complete block of receptor currents by GYKI 53655 and the absence of modulation by Concanavalin A indicated that the cells exclusively expressed non-NMDA glutamate receptors of the AMPA subtype while functional kainate receptors could not be detected. The lowest sensitivity to kainate and NBQX was observed at postnatal day (p) 18. These changes might reflect a lower abundance of GluR1 at that developmental stage. A decrease of potentiation of receptor currents by cyclothiazide (CTZ), an acceleration of the recovery from CTZ potentiation and a faster and more complete desensitization of glutamate-evoked currents suggest an up-regulation of flop splice variants with increasing age. These functional data indicate that AMPA-R expression in CA1 pyramidal neurons varies during postnatal development which can be expected to influence the kinetics of synaptic transmission and the excitotoxic vulnerability as well.</description><identifier>ISSN: 0028-3908</identifier><identifier>EISSN: 1873-7064</identifier><identifier>DOI: 10.1016/S0028-3908(99)00212-9</identifier><identifier>PMID: 10727703</identifier><identifier>CODEN: NEPHBW</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Alternative Splicing ; AMPA-receptors ; Animals ; Animals, Newborn ; Benzodiazepines - pharmacology ; Benzothiadiazines - pharmacology ; Biological and medical sciences ; CA1 pyramidal neurons ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Excitatory Amino Acid Antagonists - pharmacology ; Female ; Flip/flop splicing ; Fundamental and applied biological sciences. Psychology ; Hippocampus - cytology ; Hippocampus - growth & development ; Hippocampus - metabolism ; In Vitro Techniques ; Kinetics ; Patch clamp ; Patch-Clamp Techniques ; Pyramidal Cells - metabolism ; Quinoxalines - pharmacology ; Rats ; Receptors, AMPA - metabolism ; Receptors, AMPA - physiology ; Receptors, Kainic Acid - antagonists & inhibitors ; Receptors, Kainic Acid - metabolism ; Receptors, Kainic Acid - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuropharmacology, 2000-01, Vol.39 (6), p.931-942</ispartof><rights>2000 Elsevier Science Ltd</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-d29e9e7cb36b2833aed174a601aeabdd0647888b1e89307f24580b2b9f2c77ab3</citedby><cites>FETCH-LOGICAL-c502t-d29e9e7cb36b2833aed174a601aeabdd0647888b1e89307f24580b2b9f2c77ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0028-3908(99)00212-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1323413$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10727703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seifert, Gerald</creatorcontrib><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Dietrich, Dirk</creatorcontrib><creatorcontrib>Schumacher, Thekla B.</creatorcontrib><creatorcontrib>Dybek, Andre</creatorcontrib><creatorcontrib>Weiser, Thomas</creatorcontrib><creatorcontrib>Wienrich, Marion</creatorcontrib><creatorcontrib>Wilhelm, Doris</creatorcontrib><creatorcontrib>Steinhäuser, Christian</creatorcontrib><title>Developmental regulation of AMPA-receptor properties in CA1 pyramidal neurons of rat hippocampus</title><title>Neuropharmacology</title><addtitle>Neuropharmacology</addtitle><description>AMPA-receptor (AMPA-R) currents were recorded from CA1 pyramidal neurons in situ and after acute isolation from the hippocampus of 3- to 45-day-old rats. Membrane currents were analyzed by combining the patch clamp method with fast application techniques. The complete block of receptor currents by GYKI 53655 and the absence of modulation by Concanavalin A indicated that the cells exclusively expressed non-NMDA glutamate receptors of the AMPA subtype while functional kainate receptors could not be detected. The lowest sensitivity to kainate and NBQX was observed at postnatal day (p) 18. These changes might reflect a lower abundance of GluR1 at that developmental stage. A decrease of potentiation of receptor currents by cyclothiazide (CTZ), an acceleration of the recovery from CTZ potentiation and a faster and more complete desensitization of glutamate-evoked currents suggest an up-regulation of flop splice variants with increasing age. These functional data indicate that AMPA-R expression in CA1 pyramidal neurons varies during postnatal development which can be expected to influence the kinetics of synaptic transmission and the excitotoxic vulnerability as well.</description><subject>Alternative Splicing</subject><subject>AMPA-receptors</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Benzodiazepines - pharmacology</subject><subject>Benzothiadiazines - pharmacology</subject><subject>Biological and medical sciences</subject><subject>CA1 pyramidal neurons</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Excitatory Amino Acid Antagonists - pharmacology</subject><subject>Female</subject><subject>Flip/flop splicing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - growth & development</subject><subject>Hippocampus - metabolism</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Patch clamp</subject><subject>Patch-Clamp Techniques</subject><subject>Pyramidal Cells - metabolism</subject><subject>Quinoxalines - pharmacology</subject><subject>Rats</subject><subject>Receptors, AMPA - metabolism</subject><subject>Receptors, AMPA - physiology</subject><subject>Receptors, Kainic Acid - antagonists & inhibitors</subject><subject>Receptors, Kainic Acid - metabolism</subject><subject>Receptors, Kainic Acid - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0028-3908</issn><issn>1873-7064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxUOx2LX1IyjzIEUfpr1JZifJU1lWrYUWBfU5zWTuaGRmkiYzhX57M92l-lYIXAK_c_-cQ8gbCmcUaH3-HYDJkiuQ75X6kD-UleqArKgUvBRQVy_I6gk5Iq9S-gMAlaTyJTmiIJgQwFfk9iPeY-_DgONk-iLir7k3k_Nj4btic_NtU0a0GCYfixB9wDg5TIUbi-2GFuEhmsG1WTfiHP2YFlE0U_HbheCtGcKcTshhZ_qEr_f1mPz8_OnH9kt5_fXyaru5Lu0a2FS2TKFCYRteN0xybrClojI1UIOmadt8kJBSNhSl4iA6Vq0lNKxRHbNCmIYfk9Nd37zm3Yxp0oNLFvvejOjnpMXimqr5syAVdX6wgOsdaKNPKWKnQ3SDiQ-agl666ccM9GKwVko_ZqBV1r3dD5ibAdv_VDvTM_BuD5hkTd9FM1qX_nGc8You2MUOw2zbvcOok3U4WmxdzmTSrXfPbPIXleqjRQ</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Seifert, Gerald</creator><creator>Zhou, Min</creator><creator>Dietrich, Dirk</creator><creator>Schumacher, Thekla B.</creator><creator>Dybek, Andre</creator><creator>Weiser, Thomas</creator><creator>Wienrich, Marion</creator><creator>Wilhelm, Doris</creator><creator>Steinhäuser, Christian</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20000101</creationdate><title>Developmental regulation of AMPA-receptor properties in CA1 pyramidal neurons of rat hippocampus</title><author>Seifert, Gerald ; Zhou, Min ; Dietrich, Dirk ; Schumacher, Thekla B. ; Dybek, Andre ; Weiser, Thomas ; Wienrich, Marion ; Wilhelm, Doris ; Steinhäuser, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-d29e9e7cb36b2833aed174a601aeabdd0647888b1e89307f24580b2b9f2c77ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alternative Splicing</topic><topic>AMPA-receptors</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Benzodiazepines - pharmacology</topic><topic>Benzothiadiazines - pharmacology</topic><topic>Biological and medical sciences</topic><topic>CA1 pyramidal neurons</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Excitatory Amino Acid Antagonists - pharmacology</topic><topic>Female</topic><topic>Flip/flop splicing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - growth & development</topic><topic>Hippocampus - metabolism</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Patch clamp</topic><topic>Patch-Clamp Techniques</topic><topic>Pyramidal Cells - metabolism</topic><topic>Quinoxalines - pharmacology</topic><topic>Rats</topic><topic>Receptors, AMPA - metabolism</topic><topic>Receptors, AMPA - physiology</topic><topic>Receptors, Kainic Acid - antagonists & inhibitors</topic><topic>Receptors, Kainic Acid - metabolism</topic><topic>Receptors, Kainic Acid - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seifert, Gerald</creatorcontrib><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Dietrich, Dirk</creatorcontrib><creatorcontrib>Schumacher, Thekla B.</creatorcontrib><creatorcontrib>Dybek, Andre</creatorcontrib><creatorcontrib>Weiser, Thomas</creatorcontrib><creatorcontrib>Wienrich, Marion</creatorcontrib><creatorcontrib>Wilhelm, Doris</creatorcontrib><creatorcontrib>Steinhäuser, Christian</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuropharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seifert, Gerald</au><au>Zhou, Min</au><au>Dietrich, Dirk</au><au>Schumacher, Thekla B.</au><au>Dybek, Andre</au><au>Weiser, Thomas</au><au>Wienrich, Marion</au><au>Wilhelm, Doris</au><au>Steinhäuser, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental regulation of AMPA-receptor properties in CA1 pyramidal neurons of rat hippocampus</atitle><jtitle>Neuropharmacology</jtitle><addtitle>Neuropharmacology</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>39</volume><issue>6</issue><spage>931</spage><epage>942</epage><pages>931-942</pages><issn>0028-3908</issn><eissn>1873-7064</eissn><coden>NEPHBW</coden><abstract>AMPA-receptor (AMPA-R) currents were recorded from CA1 pyramidal neurons in situ and after acute isolation from the hippocampus of 3- to 45-day-old rats. Membrane currents were analyzed by combining the patch clamp method with fast application techniques. The complete block of receptor currents by GYKI 53655 and the absence of modulation by Concanavalin A indicated that the cells exclusively expressed non-NMDA glutamate receptors of the AMPA subtype while functional kainate receptors could not be detected. The lowest sensitivity to kainate and NBQX was observed at postnatal day (p) 18. These changes might reflect a lower abundance of GluR1 at that developmental stage. A decrease of potentiation of receptor currents by cyclothiazide (CTZ), an acceleration of the recovery from CTZ potentiation and a faster and more complete desensitization of glutamate-evoked currents suggest an up-regulation of flop splice variants with increasing age. These functional data indicate that AMPA-R expression in CA1 pyramidal neurons varies during postnatal development which can be expected to influence the kinetics of synaptic transmission and the excitotoxic vulnerability as well.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10727703</pmid><doi>10.1016/S0028-3908(99)00212-9</doi><tpages>12</tpages></addata></record> |
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| subjects | Alternative Splicing AMPA-receptors Animals Animals, Newborn Benzodiazepines - pharmacology Benzothiadiazines - pharmacology Biological and medical sciences CA1 pyramidal neurons Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Excitatory Amino Acid Antagonists - pharmacology Female Flip/flop splicing Fundamental and applied biological sciences. Psychology Hippocampus - cytology Hippocampus - growth & development Hippocampus - metabolism In Vitro Techniques Kinetics Patch clamp Patch-Clamp Techniques Pyramidal Cells - metabolism Quinoxalines - pharmacology Rats Receptors, AMPA - metabolism Receptors, AMPA - physiology Receptors, Kainic Acid - antagonists & inhibitors Receptors, Kainic Acid - metabolism Receptors, Kainic Acid - physiology Vertebrates: nervous system and sense organs |
| title | Developmental regulation of AMPA-receptor properties in CA1 pyramidal neurons of rat hippocampus |
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