Growth-rate-independent production of recombinant glucoamylase by Fusarium venenatum JeRS 325

Most recombinant proteins generated in filamentous fungi are produced in fed‐batch cultures, in which specific growth rate normally decreases progressively with time. Because of this, such cultures are more suited to the production of products that are produced efficiently at low‐growth rates (e.g.,...

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Veröffentlicht in:	Biotechnology and bioengineering 2000-05, Vol.68 (3), p.245-251
Hauptverfasser:	Wiebe, Marilyn G., Robson, Geoffrey D., Shuster, Jeff, Trinci, Anthony P.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Batch cell culture Bioconversion Biological and medical sciences Biosynthesis Biotechnology Biotechnology - instrumentation Biotechnology - methods chemostat culture Enzyme engineering Enzyme kinetics Fermentation Fundamental and applied biological sciences. Psychology Fungi Fusarium - enzymology Fusarium - genetics Fusarium - growth & development Fusarium venenatum Genetic engineering Genetic technics Glucan 1,4-alpha-Glucosidase - genetics Glucan 1,4-alpha-Glucosidase - metabolism glucoamylase Growth kinetics growth-rate-independent production Methods. Procedures. Technologies Modification of gene expression level Plant cell culture Production of selected enzymes Promoter Regions, Genetic recombinant protein Recombinant Proteins - genetics Recombinant Proteins - metabolism specific production rate
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