Effect of Shiga toxin 2 on water and ion transport in human colon in vitro
Shiga toxin-producing Escherichia coli (STEC) colonize the lower segments of the human gastrointestinal tract, causing gastrointestinal and systemic diseases. In this study, the effects of Shiga toxin 2 (Stx2) on fluid absorption and ion transport in the human colon were examined. Net water movement...
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| Veröffentlicht in: | Digestive diseases and sciences 2000-03, Vol.45 (3), p.480-486 |
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| creator | FIORITO, P BURGOS, J. M FERNANDEZ MIYAKAWA, M RIVAS, M CHILLEMI, G BERKOWSKI, D ZOTTA, E SILBERSTEIN, C IBARRA, C |
| description | Shiga toxin-producing Escherichia coli (STEC) colonize the lower segments of the human gastrointestinal tract, causing gastrointestinal and systemic diseases. In this study, the effects of Shiga toxin 2 (Stx2) on fluid absorption and ion transport in the human colon were examined. Net water movement (Jw) and short-circuit current (Isc) were simultaneously measured across the colonic mucosa incubated with crude or purified Stx2. Stx2 significantly inhibited the absorptive J(w) with no effect on the basal I(sc) after 60 min of exposure. These effects may be due to the inhibition of a nonelectrogenic transport system present in the surface colonic villus cells. Morphological studies of the colonic mucosa treated with crude or purified Stx2 demonstrated a selective damage in the absorptive villus epithelial cells. These findings suggest that Stx2 inhibits water absorption across the human colon by acting on a specific cell population: the mature, differentiated absorptive villus epithelium. |
| doi_str_mv | 10.1023/A:1005480720832 |
| format | Article |
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Morphological studies of the colonic mucosa treated with crude or purified Stx2 demonstrated a selective damage in the absorptive villus epithelial cells. These findings suggest that Stx2 inhibits water absorption across the human colon by acting on a specific cell population: the mature, differentiated absorptive villus epithelium.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1023/A:1005480720832</identifier><identifier>PMID: 10749321</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Bacterial Toxins - pharmacology ; Biological and medical sciences ; Colon - drug effects ; Colon - metabolism ; Colon - pathology ; Enterotoxins - pharmacology ; Escherichia coli ; Human bacterial diseases ; Humans ; In Vitro Techniques ; Infectious diseases ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Ion Transport - drug effects ; Medical sciences ; Shiga Toxins ; Tropical medicine ; Water - metabolism</subject><ispartof>Digestive diseases and sciences, 2000-03, Vol.45 (3), p.480-486</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright Kluwer Academic Publishers Mar 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c309t-85c15a2f7587ea4cf745a2c9f824176131c3e80b5138122faec7e298b19e62d73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1310340$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10749321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FIORITO, P</creatorcontrib><creatorcontrib>BURGOS, J. 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Stx2 significantly inhibited the absorptive J(w) with no effect on the basal I(sc) after 60 min of exposure. These effects may be due to the inhibition of a nonelectrogenic transport system present in the surface colonic villus cells. Morphological studies of the colonic mucosa treated with crude or purified Stx2 demonstrated a selective damage in the absorptive villus epithelial cells. These findings suggest that Stx2 inhibits water absorption across the human colon by acting on a specific cell population: the mature, differentiated absorptive villus epithelium.</description><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Bacterial Toxins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Colon - drug effects</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>Enterotoxins - pharmacology</subject><subject>Escherichia coli</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infectious diseases</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Ion Transport - drug effects</subject><subject>Medical sciences</subject><subject>Shiga Toxins</subject><subject>Tropical medicine</subject><subject>Water - metabolism</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkE1LAzEQhoMotlbP3iSIeFudSXY3ibdS6hcFD-p5SdPEbtluarLrx783YkXwNPPwPgzDS8gxwgUC45fjKwQocgmCgeRshwyxEDxjRSl3yRCwTDtiOSAHMa4AQAks98kAQeSKMxyS-6lz1nTUO_q4rF807fxH3VJGfUvfdWcD1e2C1om6oNu48aGjKV_2a91S45sUJHyru-APyZ7TTbRH2zkiz9fTp8ltNnu4uZuMZ5nhoLpMFgYLzZwopLA6N07kCY1ykuUoSuRouJUwL5BLZMxpa4RlSs5R2ZItBB-R85-7m-Bfexu7al1HY5tGt9b3sRIICEp9i6f_xJXvQ5t-qxjmnOeSlUk62Ur9fG0X1SbUax0-q9-KknC2FXQ0unGpBlPHP48j8Bz4F3T-cgA</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>FIORITO, P</creator><creator>BURGOS, J. 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M</au><au>FERNANDEZ MIYAKAWA, M</au><au>RIVAS, M</au><au>CHILLEMI, G</au><au>BERKOWSKI, D</au><au>ZOTTA, E</au><au>SILBERSTEIN, C</au><au>IBARRA, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Shiga toxin 2 on water and ion transport in human colon in vitro</atitle><jtitle>Digestive diseases and sciences</jtitle><addtitle>Dig Dis Sci</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>45</volume><issue>3</issue><spage>480</spage><epage>486</epage><pages>480-486</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract>Shiga toxin-producing Escherichia coli (STEC) colonize the lower segments of the human gastrointestinal tract, causing gastrointestinal and systemic diseases. In this study, the effects of Shiga toxin 2 (Stx2) on fluid absorption and ion transport in the human colon were examined. Net water movement (Jw) and short-circuit current (Isc) were simultaneously measured across the colonic mucosa incubated with crude or purified Stx2. Stx2 significantly inhibited the absorptive J(w) with no effect on the basal I(sc) after 60 min of exposure. These effects may be due to the inhibition of a nonelectrogenic transport system present in the surface colonic villus cells. Morphological studies of the colonic mucosa treated with crude or purified Stx2 demonstrated a selective damage in the absorptive villus epithelial cells. These findings suggest that Stx2 inhibits water absorption across the human colon by acting on a specific cell population: the mature, differentiated absorptive villus epithelium.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>10749321</pmid><doi>10.1023/A:1005480720832</doi><tpages>7</tpages></addata></record> |
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| subjects | Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacterial Toxins - pharmacology Biological and medical sciences Colon - drug effects Colon - metabolism Colon - pathology Enterotoxins - pharmacology Escherichia coli Human bacterial diseases Humans In Vitro Techniques Infectious diseases Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Ion Transport - drug effects Medical sciences Shiga Toxins Tropical medicine Water - metabolism |
| title | Effect of Shiga toxin 2 on water and ion transport in human colon in vitro |
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