Metabolic disorder in myocardiac intracellular free calcium after thermal injury
Cutaneous burn trauma causes functional inhibition of the heart, but the mechanism is unclear. Using a high dissociation constant ( K D) calcium indicator TF-BAPTA and 19F MR spectroscopy, the relationship between the changes of cytosolic free calcium and cardiac function after burn trauma was exami...
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| Veröffentlicht in: | Burns 2001-08, Vol.27 (5), p.453-457 |
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| creator | Xia, Zhao-fan Horton, J.W. Tang, Hong-tai Yang, Yong |
| description | Cutaneous burn trauma causes functional inhibition of the heart, but the mechanism is unclear. Using a high dissociation constant (
K
D) calcium indicator TF-BAPTA and
19F MR spectroscopy, the relationship between the changes of cytosolic free calcium and cardiac function after burn trauma was examined. Sprague–Dawley rats received scald (43% TBSA) or sham burns. Twenty-four hours later, the hearts were excised and perfused by the Langendorff method with a modified phosphate-free Krebs–Henseleit bicarbonate buffer. Left ventricular developed pressure (LVDP) was recorded through a catheter attached to an intraventricular balloon. At the same time,
31P and
19F nuclear magnetic resonance (NMR) spectroscopy was perforined before and after TF-BAPTA loading. LVDP of the heart from burned rats was 40% less than in sham burn rats (65±6 vs 110±12 mmHg,
P |
| doi_str_mv | 10.1016/S0305-4179(00)00119-4 |
| format | Article |
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K
D) calcium indicator TF-BAPTA and
19F MR spectroscopy, the relationship between the changes of cytosolic free calcium and cardiac function after burn trauma was examined. Sprague–Dawley rats received scald (43% TBSA) or sham burns. Twenty-four hours later, the hearts were excised and perfused by the Langendorff method with a modified phosphate-free Krebs–Henseleit bicarbonate buffer. Left ventricular developed pressure (LVDP) was recorded through a catheter attached to an intraventricular balloon. At the same time,
31P and
19F nuclear magnetic resonance (NMR) spectroscopy was perforined before and after TF-BAPTA loading. LVDP of the heart from burned rats was 40% less than in sham burn rats (65±6 vs 110±12 mmHg,
P<0.01). Cytosolic free calcium increased about four-fold in those hearts from the burn group compared to the sham burn group (0.807±0.192 vs 3.891±0.929 μM). Loading TF-BAPTA in those hearts only caused about a 15–20% decrease in LVDP. PCr/Pi ratio also decreased significantly with this loading, but ATP signals were not affected. In conclusion, the inhibition of cardiac contractility caused by burn trauma correlated with the overload of cytosolic free calcium in the heart.</description><identifier>ISSN: 0305-4179</identifier><identifier>EISSN: 1879-1409</identifier><identifier>DOI: 10.1016/S0305-4179(00)00119-4</identifier><identifier>PMID: 11451597</identifier><identifier>CODEN: BURND8</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>19F MRS ; Adenosine Triphosphate - metabolism ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Biological and medical sciences ; Burn trauma ; Burns - complications ; Calcium - metabolism ; Calcium Metabolism Disorders - diagnosis ; Calcium Metabolism Disorders - etiology ; Cardiomyopathies - diagnosis ; Cardiomyopathies - etiology ; Cardiomyopathies - metabolism ; Chelating Agents - pharmacology ; Cytosolic free calcium ; Disease Models, Animal ; Egtazic Acid - analogs & derivatives ; Emergency and intensive care: burns ; Heart ; Intensive care medicine ; Magnetic Resonance Spectroscopy ; Male ; Medical sciences ; Myocardium - chemistry ; Myocardium - metabolism ; Perfusion ; Probability ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reference Values ; Sensitivity and Specificity ; TF-BAPTA ; Ventricular Dysfunction, Left - diagnosis ; Ventricular Dysfunction, Left - etiology</subject><ispartof>Burns, 2001-08, Vol.27 (5), p.453-457</ispartof><rights>2001 Elsevier Science Ltd and ISBI</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-8b365d4f727a88d0255003d0fbbb5e119b2f28cdcae0dfa97163a3d9b26e4bd23</citedby><cites>FETCH-LOGICAL-c390t-8b365d4f727a88d0255003d0fbbb5e119b2f28cdcae0dfa97163a3d9b26e4bd23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0305417900001194$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1066314$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11451597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xia, Zhao-fan</creatorcontrib><creatorcontrib>Horton, J.W.</creatorcontrib><creatorcontrib>Tang, Hong-tai</creatorcontrib><creatorcontrib>Yang, Yong</creatorcontrib><title>Metabolic disorder in myocardiac intracellular free calcium after thermal injury</title><title>Burns</title><addtitle>Burns</addtitle><description>Cutaneous burn trauma causes functional inhibition of the heart, but the mechanism is unclear. Using a high dissociation constant (
K
D) calcium indicator TF-BAPTA and
19F MR spectroscopy, the relationship between the changes of cytosolic free calcium and cardiac function after burn trauma was examined. Sprague–Dawley rats received scald (43% TBSA) or sham burns. Twenty-four hours later, the hearts were excised and perfused by the Langendorff method with a modified phosphate-free Krebs–Henseleit bicarbonate buffer. Left ventricular developed pressure (LVDP) was recorded through a catheter attached to an intraventricular balloon. At the same time,
31P and
19F nuclear magnetic resonance (NMR) spectroscopy was perforined before and after TF-BAPTA loading. LVDP of the heart from burned rats was 40% less than in sham burn rats (65±6 vs 110±12 mmHg,
P<0.01). Cytosolic free calcium increased about four-fold in those hearts from the burn group compared to the sham burn group (0.807±0.192 vs 3.891±0.929 μM). Loading TF-BAPTA in those hearts only caused about a 15–20% decrease in LVDP. PCr/Pi ratio also decreased significantly with this loading, but ATP signals were not affected. In conclusion, the inhibition of cardiac contractility caused by burn trauma correlated with the overload of cytosolic free calcium in the heart.</description><subject>19F MRS</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Burn trauma</subject><subject>Burns - complications</subject><subject>Calcium - metabolism</subject><subject>Calcium Metabolism Disorders - diagnosis</subject><subject>Calcium Metabolism Disorders - etiology</subject><subject>Cardiomyopathies - diagnosis</subject><subject>Cardiomyopathies - etiology</subject><subject>Cardiomyopathies - metabolism</subject><subject>Chelating Agents - pharmacology</subject><subject>Cytosolic free calcium</subject><subject>Disease Models, Animal</subject><subject>Egtazic Acid - analogs & derivatives</subject><subject>Emergency and intensive care: burns</subject><subject>Heart</subject><subject>Intensive care medicine</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Myocardium - chemistry</subject><subject>Myocardium - metabolism</subject><subject>Perfusion</subject><subject>Probability</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Values</subject><subject>Sensitivity and Specificity</subject><subject>TF-BAPTA</subject><subject>Ventricular Dysfunction, Left - diagnosis</subject><subject>Ventricular Dysfunction, Left - etiology</subject><issn>0305-4179</issn><issn>1879-1409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMouq7-BKUHET1UJ23Tj5OI-AWKgnoO02SCWdrtmrTC_ntTd1Fv5jIkPPNm5mHsgMMZB56fv0AKIs54UZ0AnAJwXsXZBpvwsqhinkG1ySY_yA7b9X4G4YgSttkO55ngoiom7PmReqy7xqpIW985TS6y86hddgqdtqjCrXeoqGmGBl1kHFGksFF2aCM0fcD7d3ItNgGcDW65x7YMNp7213XK3m6uX6_u4oen2_ury4dYpRX0cVmnudCZKZICy1JDIgRAqsHUdS0oLFMnJimVVkigDVYFz1NMdXjOKat1kk7Z8Sp34bqPgXwvW-vHMXFO3eBlwQHy4CGAYgUq13nvyMiFsy26peQgR5XyW6UcPUkA-a1SZqHvcP3BULekf7vW7gJwtAbQByPG4VxZ_yc9z1M-5lysMAo2Pi056ZWluSJtHale6s7-M8kX04uQtA</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Xia, Zhao-fan</creator><creator>Horton, J.W.</creator><creator>Tang, Hong-tai</creator><creator>Yang, Yong</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Metabolic disorder in myocardiac intracellular free calcium after thermal injury</title><author>Xia, Zhao-fan ; Horton, J.W. ; Tang, Hong-tai ; Yang, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-8b365d4f727a88d0255003d0fbbb5e119b2f28cdcae0dfa97163a3d9b26e4bd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>19F MRS</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Burn trauma</topic><topic>Burns - complications</topic><topic>Calcium - metabolism</topic><topic>Calcium Metabolism Disorders - diagnosis</topic><topic>Calcium Metabolism Disorders - etiology</topic><topic>Cardiomyopathies - diagnosis</topic><topic>Cardiomyopathies - etiology</topic><topic>Cardiomyopathies - metabolism</topic><topic>Chelating Agents - pharmacology</topic><topic>Cytosolic free calcium</topic><topic>Disease Models, Animal</topic><topic>Egtazic Acid - analogs & derivatives</topic><topic>Emergency and intensive care: burns</topic><topic>Heart</topic><topic>Intensive care medicine</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Myocardium - chemistry</topic><topic>Myocardium - metabolism</topic><topic>Perfusion</topic><topic>Probability</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Values</topic><topic>Sensitivity and Specificity</topic><topic>TF-BAPTA</topic><topic>Ventricular Dysfunction, Left - diagnosis</topic><topic>Ventricular Dysfunction, Left - etiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Zhao-fan</creatorcontrib><creatorcontrib>Horton, J.W.</creatorcontrib><creatorcontrib>Tang, Hong-tai</creatorcontrib><creatorcontrib>Yang, Yong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Burns</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, Zhao-fan</au><au>Horton, J.W.</au><au>Tang, Hong-tai</au><au>Yang, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic disorder in myocardiac intracellular free calcium after thermal injury</atitle><jtitle>Burns</jtitle><addtitle>Burns</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>27</volume><issue>5</issue><spage>453</spage><epage>457</epage><pages>453-457</pages><issn>0305-4179</issn><eissn>1879-1409</eissn><coden>BURND8</coden><abstract>Cutaneous burn trauma causes functional inhibition of the heart, but the mechanism is unclear. Using a high dissociation constant (
K
D) calcium indicator TF-BAPTA and
19F MR spectroscopy, the relationship between the changes of cytosolic free calcium and cardiac function after burn trauma was examined. Sprague–Dawley rats received scald (43% TBSA) or sham burns. Twenty-four hours later, the hearts were excised and perfused by the Langendorff method with a modified phosphate-free Krebs–Henseleit bicarbonate buffer. Left ventricular developed pressure (LVDP) was recorded through a catheter attached to an intraventricular balloon. At the same time,
31P and
19F nuclear magnetic resonance (NMR) spectroscopy was perforined before and after TF-BAPTA loading. LVDP of the heart from burned rats was 40% less than in sham burn rats (65±6 vs 110±12 mmHg,
P<0.01). Cytosolic free calcium increased about four-fold in those hearts from the burn group compared to the sham burn group (0.807±0.192 vs 3.891±0.929 μM). Loading TF-BAPTA in those hearts only caused about a 15–20% decrease in LVDP. PCr/Pi ratio also decreased significantly with this loading, but ATP signals were not affected. In conclusion, the inhibition of cardiac contractility caused by burn trauma correlated with the overload of cytosolic free calcium in the heart.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11451597</pmid><doi>10.1016/S0305-4179(00)00119-4</doi><tpages>5</tpages></addata></record> |
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| subjects | 19F MRS Adenosine Triphosphate - metabolism Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Biological and medical sciences Burn trauma Burns - complications Calcium - metabolism Calcium Metabolism Disorders - diagnosis Calcium Metabolism Disorders - etiology Cardiomyopathies - diagnosis Cardiomyopathies - etiology Cardiomyopathies - metabolism Chelating Agents - pharmacology Cytosolic free calcium Disease Models, Animal Egtazic Acid - analogs & derivatives Emergency and intensive care: burns Heart Intensive care medicine Magnetic Resonance Spectroscopy Male Medical sciences Myocardium - chemistry Myocardium - metabolism Perfusion Probability Random Allocation Rats Rats, Sprague-Dawley Reference Values Sensitivity and Specificity TF-BAPTA Ventricular Dysfunction, Left - diagnosis Ventricular Dysfunction, Left - etiology |
| title | Metabolic disorder in myocardiac intracellular free calcium after thermal injury |
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