Size-exclusion chromatography study of the molecular-weight distribution of γ-irradiated pullulan

The polyelectrolyte behavior of γ-irradiated pullulan in aqueous solutions leads to secondary effects of adsorption on a Shodex OHPak KB 806 column gel during size-exclusion chromatography. Suppression of the polyelectrolyte properties of γ-irradiated pullulan is achieved by using a 0.05 M aqueous s...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Carbohydrate research 2000-03, Vol.324 (4), p.283-287
Hauptverfasser:	Shingel, Kirill I, Tsarenkov, Valery M, Petrov, Petr T
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Gel Electrolytes Gamma Rays Glucans - chemistry Glucans - radiation effects High-performance size-exclusion chromatography Molecular Weight Molecular-weight distribution Viscosity γ-Irradiated pullulan
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The polyelectrolyte behavior of γ-irradiated pullulan in aqueous solutions leads to secondary effects of adsorption on a Shodex OHPak KB 806 column gel during size-exclusion chromatography. Suppression of the polyelectrolyte properties of γ-irradiated pullulan is achieved by using a 0.05 M aqueous solution of NaH 2PO 4 (pH 4.95) as the mobile phase. Under these conditions, adequate molecular-weight distributions of γ-irradiated pullulan samples are obtained.
ISSN:	0008-6215 1873-426X
DOI:	10.1016/S0008-6215(99)00305-5