Nematode neuropeptide modulation of the vagina vera of Ascaris suum: in vitro effects of PF1, PF2, PF4, AF3 and AF4
Ascaris suum possesses a large number of FMRFamide-related peptides (FaRPs) of which KNEFIRFamide (AF1), KHEYLRFamide (AF2) and KSAYMRFamide (AF8/PF3) have been shown to modulate the intrinsic, rhythmic activity of the vagina vera of A. suum in vitro. In the present study, the effects of the nematod...
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| Veröffentlicht in: | Parasitology 2000-01, Vol.120 (1), p.79-89 |
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| creator | FELLOWES, R. A. MAULE, A. G. MARKS, N. J. GEARY, T. G. THOMPSON, D. P. HALTON, D. W. |
| description | Ascaris suum possesses a large number of FMRFamide-related peptides (FaRPs) of which KNEFIRFamide (AF1),
KHEYLRFamide (AF2) and KSAYMRFamide (AF8/PF3) have been shown to modulate the intrinsic, rhythmic activity
of the vagina vera of A. suum in vitro. In the present study, the effects of the nematode FaRPs, SDPNFLRFamide (PF1),
SADPNFLREamide (PF2) and KPNFIRFamide (PF4) (from Panagrellus redivivus) and AVPGVLRFamide (AF3) and
GDVPGVLRFamide (AF4) (from A. suum) on the in vitro activity of the vagina vera were examined. The effects of each
of the peptides were qualitatively and quantitatively distinct. All 3 FaRPs from P. redivivus were inhibitory, causing a
cessation of contractions. PF2 was 3 times more potent than PF1, with a threshold of 1 nM. Although PF4 was the least
potent (threshold, 10 nM), its effects at [ges ]10 nM were quantitatively the greatest. Both AF3 and AF4 (1 μM) induced
complex, multiphasic responses consisting of an initial contraction and spastic paralysis followed by a return of contractile
activity of increased amplitude. AF3 was 3 times more potent than AF4. The effects of these peptides had some similarities
to those observed on A. suum somatic body wall muscle in vitro, with PF1, PF2 and PF4 being inhibitory and AF3 and
AF4 being excitatory. |
| doi_str_mv | 10.1017/S0031182099005260 |
| format | Article |
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KHEYLRFamide (AF2) and KSAYMRFamide (AF8/PF3) have been shown to modulate the intrinsic, rhythmic activity
of the vagina vera of A. suum in vitro. In the present study, the effects of the nematode FaRPs, SDPNFLRFamide (PF1),
SADPNFLREamide (PF2) and KPNFIRFamide (PF4) (from Panagrellus redivivus) and AVPGVLRFamide (AF3) and
GDVPGVLRFamide (AF4) (from A. suum) on the in vitro activity of the vagina vera were examined. The effects of each
of the peptides were qualitatively and quantitatively distinct. All 3 FaRPs from P. redivivus were inhibitory, causing a
cessation of contractions. PF2 was 3 times more potent than PF1, with a threshold of 1 nM. Although PF4 was the least
potent (threshold, 10 nM), its effects at [ges ]10 nM were quantitatively the greatest. Both AF3 and AF4 (1 μM) induced
complex, multiphasic responses consisting of an initial contraction and spastic paralysis followed by a return of contractile
activity of increased amplitude. AF3 was 3 times more potent than AF4. The effects of these peptides had some similarities
to those observed on A. suum somatic body wall muscle in vitro, with PF1, PF2 and PF4 being inhibitory and AF3 and
AF4 being excitatory.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182099005260</identifier><identifier>PMID: 10726269</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge: Cambridge University Press</publisher><subject>Animals ; Ascaris suum ; Ascaris suum - chemistry ; Ascaris suum - physiology ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Dose-Response Relationship, Drug ; FaRP ; Female ; FMRFamide - analogs & derivatives ; FMRFamide - physiology ; Fundamental and applied biological sciences. Psychology ; gonoduct ; Invertebrates ; muscle activity ; Muscle Contraction - drug effects ; Muscle Contraction - physiology ; Muscle, Smooth - drug effects ; Muscle, Smooth - physiology ; Nemathelminthia. Plathelmintha ; nematode ; neuropeptides ; Physiology. Development ; reproductive system ; RFamide ; vagina ; Vagina - physiology</subject><ispartof>Parasitology, 2000-01, Vol.120 (1), p.79-89</ispartof><rights>2000 Cambridge University Press</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-9a689a248af3c6f1e92020ca783722bdea15dd7c61b58070b90a0ebb49a39a733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182099005260/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,4024,27923,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1272229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10726269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FELLOWES, R. A.</creatorcontrib><creatorcontrib>MAULE, A. G.</creatorcontrib><creatorcontrib>MARKS, N. J.</creatorcontrib><creatorcontrib>GEARY, T. G.</creatorcontrib><creatorcontrib>THOMPSON, D. P.</creatorcontrib><creatorcontrib>HALTON, D. W.</creatorcontrib><title>Nematode neuropeptide modulation of the vagina vera of Ascaris suum: in vitro effects of PF1, PF2, PF4, AF3 and AF4</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Ascaris suum possesses a large number of FMRFamide-related peptides (FaRPs) of which KNEFIRFamide (AF1),
KHEYLRFamide (AF2) and KSAYMRFamide (AF8/PF3) have been shown to modulate the intrinsic, rhythmic activity
of the vagina vera of A. suum in vitro. In the present study, the effects of the nematode FaRPs, SDPNFLRFamide (PF1),
SADPNFLREamide (PF2) and KPNFIRFamide (PF4) (from Panagrellus redivivus) and AVPGVLRFamide (AF3) and
GDVPGVLRFamide (AF4) (from A. suum) on the in vitro activity of the vagina vera were examined. The effects of each
of the peptides were qualitatively and quantitatively distinct. All 3 FaRPs from P. redivivus were inhibitory, causing a
cessation of contractions. PF2 was 3 times more potent than PF1, with a threshold of 1 nM. Although PF4 was the least
potent (threshold, 10 nM), its effects at [ges ]10 nM were quantitatively the greatest. Both AF3 and AF4 (1 μM) induced
complex, multiphasic responses consisting of an initial contraction and spastic paralysis followed by a return of contractile
activity of increased amplitude. AF3 was 3 times more potent than AF4. The effects of these peptides had some similarities
to those observed on A. suum somatic body wall muscle in vitro, with PF1, PF2 and PF4 being inhibitory and AF3 and
AF4 being excitatory.</description><subject>Animals</subject><subject>Ascaris suum</subject><subject>Ascaris suum - chemistry</subject><subject>Ascaris suum - physiology</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Dose-Response Relationship, Drug</subject><subject>FaRP</subject><subject>Female</subject><subject>FMRFamide - analogs & derivatives</subject><subject>FMRFamide - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gonoduct</subject><subject>Invertebrates</subject><subject>muscle activity</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - physiology</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>nematode</subject><subject>neuropeptides</subject><subject>Physiology. Development</subject><subject>reproductive system</subject><subject>RFamide</subject><subject>vagina</subject><subject>Vagina - physiology</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2P0zAQQC0EYsvCD-CCfECcNjC2UzvmVla0i1TxoQWu1iSZLF6auNhJBf9-HbUCJCQuM7LnzXj8GHsq4KUAYV5dAyghKgnWAiylhntsIUpti0pocZ8t5nIx18_Yo5RuAUArLR-yMwFGaqntgqX31OMYWuIDTTHsaT_6fOhDO-1w9GHgoePjN-IHvPED8gNFnK9WqcHoE0_T1L_mfuAHP8bAqeuoGdNMfFyLixzkHMoLvlorjkObc_mYPehwl-jJKZ-zL-u3ny-viu2HzbvL1bZoSqjGwqKuLMqywk41uhNkJUho0FTKSFm3hGLZtqbRol5WYKC2gEB1XVpUFo1S5-zFce4-hh8TpdH1PjW02-FAYUrOiGxEVTqD4gg2MaQUqXP76HuMv5wAN5t2_5jOPc9Ow6e6p_avjqPaDDw_AZhd7bqIQ-PTH07mT8gZK46YTyP9_F3G-N1po8zS6c0n99Vsr99swLj5XXXaFfs6-vaG3G2Y4pBF_mfbO0NgoJI</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>FELLOWES, R. A.</creator><creator>MAULE, A. G.</creator><creator>MARKS, N. J.</creator><creator>GEARY, T. G.</creator><creator>THOMPSON, D. P.</creator><creator>HALTON, D. W.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Nematode neuropeptide modulation of the vagina vera of Ascaris suum: in vitro effects of PF1, PF2, PF4, AF3 and AF4</title><author>FELLOWES, R. A. ; MAULE, A. G. ; MARKS, N. J. ; GEARY, T. G. ; THOMPSON, D. P. ; HALTON, D. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-9a689a248af3c6f1e92020ca783722bdea15dd7c61b58070b90a0ebb49a39a733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Ascaris suum</topic><topic>Ascaris suum - chemistry</topic><topic>Ascaris suum - physiology</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Dose-Response Relationship, Drug</topic><topic>FaRP</topic><topic>Female</topic><topic>FMRFamide - analogs & derivatives</topic><topic>FMRFamide - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gonoduct</topic><topic>Invertebrates</topic><topic>muscle activity</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Contraction - physiology</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - physiology</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>nematode</topic><topic>neuropeptides</topic><topic>Physiology. Development</topic><topic>reproductive system</topic><topic>RFamide</topic><topic>vagina</topic><topic>Vagina - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FELLOWES, R. A.</creatorcontrib><creatorcontrib>MAULE, A. G.</creatorcontrib><creatorcontrib>MARKS, N. J.</creatorcontrib><creatorcontrib>GEARY, T. G.</creatorcontrib><creatorcontrib>THOMPSON, D. P.</creatorcontrib><creatorcontrib>HALTON, D. W.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FELLOWES, R. A.</au><au>MAULE, A. G.</au><au>MARKS, N. J.</au><au>GEARY, T. G.</au><au>THOMPSON, D. P.</au><au>HALTON, D. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nematode neuropeptide modulation of the vagina vera of Ascaris suum: in vitro effects of PF1, PF2, PF4, AF3 and AF4</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2000-01</date><risdate>2000</risdate><volume>120</volume><issue>1</issue><spage>79</spage><epage>89</epage><pages>79-89</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Ascaris suum possesses a large number of FMRFamide-related peptides (FaRPs) of which KNEFIRFamide (AF1),
KHEYLRFamide (AF2) and KSAYMRFamide (AF8/PF3) have been shown to modulate the intrinsic, rhythmic activity
of the vagina vera of A. suum in vitro. In the present study, the effects of the nematode FaRPs, SDPNFLRFamide (PF1),
SADPNFLREamide (PF2) and KPNFIRFamide (PF4) (from Panagrellus redivivus) and AVPGVLRFamide (AF3) and
GDVPGVLRFamide (AF4) (from A. suum) on the in vitro activity of the vagina vera were examined. The effects of each
of the peptides were qualitatively and quantitatively distinct. All 3 FaRPs from P. redivivus were inhibitory, causing a
cessation of contractions. PF2 was 3 times more potent than PF1, with a threshold of 1 nM. Although PF4 was the least
potent (threshold, 10 nM), its effects at [ges ]10 nM were quantitatively the greatest. Both AF3 and AF4 (1 μM) induced
complex, multiphasic responses consisting of an initial contraction and spastic paralysis followed by a return of contractile
activity of increased amplitude. AF3 was 3 times more potent than AF4. The effects of these peptides had some similarities
to those observed on A. suum somatic body wall muscle in vitro, with PF1, PF2 and PF4 being inhibitory and AF3 and
AF4 being excitatory.</abstract><cop>Cambridge</cop><pub>Cambridge University Press</pub><pmid>10726269</pmid><doi>10.1017/S0031182099005260</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Parasitology, 2000-01, Vol.120 (1), p.79-89 |
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| source | MEDLINE; Cambridge Journals |
| subjects | Animals Ascaris suum Ascaris suum - chemistry Ascaris suum - physiology Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Dose-Response Relationship, Drug FaRP Female FMRFamide - analogs & derivatives FMRFamide - physiology Fundamental and applied biological sciences. Psychology gonoduct Invertebrates muscle activity Muscle Contraction - drug effects Muscle Contraction - physiology Muscle, Smooth - drug effects Muscle, Smooth - physiology Nemathelminthia. Plathelmintha nematode neuropeptides Physiology. Development reproductive system RFamide vagina Vagina - physiology |
| title | Nematode neuropeptide modulation of the vagina vera of Ascaris suum: in vitro effects of PF1, PF2, PF4, AF3 and AF4 |
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