Deactivation of cultured human liver myofibroblasts by Trans‐resveratrol, a grapevine‐derived polyphenol
Liver myofibroblasts are major actors in the development of liver fibrosis and cancer progression. There is a large interest in drugs that might deactivate these cells. Many studies have shown that the grapevine‐derived polyphenol, trans‐resveratrol, and other stilbenes have therapeutic potential in...
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| Veröffentlicht in: | Hepatology (Baltimore, Md.) Md.), 2000-04, Vol.31 (4), p.922-931 |
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| container_title | Hepatology (Baltimore, Md.) |
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| creator | Godichaud, Sandrine Krisa, Stéphanie Couronné, Baptiste Dubuisson, Liliane Mérillon, Jean‐Michel Desmoulière, Alexis Rosenbaum, Jean |
| description | Liver myofibroblasts are major actors in the development of liver fibrosis and cancer progression. There is a large interest in drugs that might deactivate these cells. Many studies have shown that the grapevine‐derived polyphenol, trans‐resveratrol, and other stilbenes have therapeutic potential in some diseases. In this work, we have studied the effect of grapevine polyphenols on cultured human liver myofibroblasts. We have shown that trans‐resveratrol profoundly affects myofibroblast phenotype. Trans‐resveratrol induced morphological modifications. It markedly reduced proliferation of myofibroblasts in a dose‐dependent manner. Trans‐resveratrol also decreased the expression of α smooth muscle actin (α‐SMA) without affecting vimentin or β‐cytoplasmic actin expression. It decreased myofibroblast migration in a monolayer wounding assay. We also showed that trans‐resveratrol inhibited the messenger RNA (mRNA) expression of type I collagen. Finally, it decreased the secretion of matrix metalloproteinase 2 (MMP‐2). We conclude that
trans‐resveratrol can deactivate human liver myofibroblasts. In the second part of this study, we have shown that neither
trans‐piceid (a glycosylated analog) nor
trans‐piceatannol (a hydroxylated analog) reproduces
trans‐resveratrol effects on liver myofibroblasts. We finally show that, although trans‐resveratrol decreases the proliferation of skin fibroblast and vascular smooth muscle cells, it does not affect their expression of α‐SMA, which indicates some cell specificity. |
| doi_str_mv | 10.1053/he.2000.5848 |
| format | Article |
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trans‐resveratrol can deactivate human liver myofibroblasts. In the second part of this study, we have shown that neither
trans‐piceid (a glycosylated analog) nor
trans‐piceatannol (a hydroxylated analog) reproduces
trans‐resveratrol effects on liver myofibroblasts. We finally show that, although trans‐resveratrol decreases the proliferation of skin fibroblast and vascular smooth muscle cells, it does not affect their expression of α‐SMA, which indicates some cell specificity.</description><identifier>ISSN: 0270-9139</identifier><identifier>EISSN: 1527-3350</identifier><identifier>DOI: 10.1053/he.2000.5848</identifier><identifier>PMID: 10733549</identifier><identifier>CODEN: HPTLD9</identifier><language>eng</language><publisher>Philadelphia, PA: W.B. Saunders</publisher><subject>Actins - analysis ; Antioxidants - pharmacology ; Biological and medical sciences ; Cell Division - drug effects ; Cell Movement - drug effects ; Cells, Cultured ; Enzyme Activation - drug effects ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fibroblasts - ultrastructure ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression - drug effects ; Humans ; Liver - cytology ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Matrix Metalloproteinase 2 - metabolism ; Medical sciences ; Microscopy, Electron ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 ; Mitogen-Activated Protein Kinases - metabolism ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Other diseases. Semiology ; Procollagen - genetics ; RNA, Messenger - analysis ; Stem Cells - drug effects ; Stem Cells - metabolism ; Stem Cells - ultrastructure ; Stilbenes - pharmacology</subject><ispartof>Hepatology (Baltimore, Md.), 2000-04, Vol.31 (4), p.922-931</ispartof><rights>Copyright © 2000 American Association for the Study of Liver Diseases</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3206-f80f2073db9d4ab1f66657f12dbe23c4048b866624586f9c379ebf6e7093a1513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1053%2Fhe.2000.5848$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1053%2Fhe.2000.5848$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1309437$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10733549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Godichaud, Sandrine</creatorcontrib><creatorcontrib>Krisa, Stéphanie</creatorcontrib><creatorcontrib>Couronné, Baptiste</creatorcontrib><creatorcontrib>Dubuisson, Liliane</creatorcontrib><creatorcontrib>Mérillon, Jean‐Michel</creatorcontrib><creatorcontrib>Desmoulière, Alexis</creatorcontrib><creatorcontrib>Rosenbaum, Jean</creatorcontrib><title>Deactivation of cultured human liver myofibroblasts by Trans‐resveratrol, a grapevine‐derived polyphenol</title><title>Hepatology (Baltimore, Md.)</title><addtitle>Hepatology</addtitle><description>Liver myofibroblasts are major actors in the development of liver fibrosis and cancer progression. There is a large interest in drugs that might deactivate these cells. Many studies have shown that the grapevine‐derived polyphenol, trans‐resveratrol, and other stilbenes have therapeutic potential in some diseases. In this work, we have studied the effect of grapevine polyphenols on cultured human liver myofibroblasts. We have shown that trans‐resveratrol profoundly affects myofibroblast phenotype. Trans‐resveratrol induced morphological modifications. It markedly reduced proliferation of myofibroblasts in a dose‐dependent manner. Trans‐resveratrol also decreased the expression of α smooth muscle actin (α‐SMA) without affecting vimentin or β‐cytoplasmic actin expression. It decreased myofibroblast migration in a monolayer wounding assay. We also showed that trans‐resveratrol inhibited the messenger RNA (mRNA) expression of type I collagen. Finally, it decreased the secretion of matrix metalloproteinase 2 (MMP‐2). We conclude that
trans‐resveratrol can deactivate human liver myofibroblasts. In the second part of this study, we have shown that neither
trans‐piceid (a glycosylated analog) nor
trans‐piceatannol (a hydroxylated analog) reproduces
trans‐resveratrol effects on liver myofibroblasts. We finally show that, although trans‐resveratrol decreases the proliferation of skin fibroblast and vascular smooth muscle cells, it does not affect their expression of α‐SMA, which indicates some cell specificity.</description><subject>Actins - analysis</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cell Movement - drug effects</subject><subject>Cells, Cultured</subject><subject>Enzyme Activation - drug effects</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - ultrastructure</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression - drug effects</subject><subject>Humans</subject><subject>Liver - cytology</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 3</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Other diseases. Semiology</subject><subject>Procollagen - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><subject>Stem Cells - ultrastructure</subject><subject>Stilbenes - pharmacology</subject><issn>0270-9139</issn><issn>1527-3350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90b1uFDEQB3ALgcgR6KiRC0SVvYzt_SxREghSpKQItWV7x5yRd73Yu4e24xF4xjwJPt1JUFFZ8vw8M_qbkLcMtgwqcbnDLQeAbdWW7TOyYRVvCiEqeE42wBsoOia6M_Iqpe9ZdSVvX5IzBk0mZbch_hqVmd1ezS6MNFhqFj8vEXu6WwY1Uu_2GOmwBut0DNqrNCeqV_oY1Ziefv2OmDJQcwz-gir6LaoJ927EXOox5sc9nYJfpx2Owb8mL6zyCd-cznPy9dPN49VtcXf_-cvVx7vCCA51YVuwPG_Y664vlWa2ruuqsYz3GrkwJZStbvMdL6u2tp0RTYfa1thAJxSrmDgnH459pxh-LJhmObhk0Hs1YliSzLBrAESGF0doYkgpopVTdIOKq2QgD-nKHcpDuvKQbubvTn0XPWD_Dz7GmcH7E1DJKG9zSMalv07kDxBNZvWR_XQe1__OlLc3DxUDwaBktfgDT3uVRw</recordid><startdate>200004</startdate><enddate>200004</enddate><creator>Godichaud, Sandrine</creator><creator>Krisa, Stéphanie</creator><creator>Couronné, Baptiste</creator><creator>Dubuisson, Liliane</creator><creator>Mérillon, Jean‐Michel</creator><creator>Desmoulière, Alexis</creator><creator>Rosenbaum, Jean</creator><general>W.B. Saunders</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200004</creationdate><title>Deactivation of cultured human liver myofibroblasts by Trans‐resveratrol, a grapevine‐derived polyphenol</title><author>Godichaud, Sandrine ; Krisa, Stéphanie ; Couronné, Baptiste ; Dubuisson, Liliane ; Mérillon, Jean‐Michel ; Desmoulière, Alexis ; Rosenbaum, Jean</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3206-f80f2073db9d4ab1f66657f12dbe23c4048b866624586f9c379ebf6e7093a1513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Actins - analysis</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cell Movement - drug effects</topic><topic>Cells, Cultured</topic><topic>Enzyme Activation - drug effects</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - ultrastructure</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Expression - drug effects</topic><topic>Humans</topic><topic>Liver - cytology</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Other diseases. Semiology</topic><topic>Procollagen - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><topic>Stem Cells - ultrastructure</topic><topic>Stilbenes - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Godichaud, Sandrine</creatorcontrib><creatorcontrib>Krisa, Stéphanie</creatorcontrib><creatorcontrib>Couronné, Baptiste</creatorcontrib><creatorcontrib>Dubuisson, Liliane</creatorcontrib><creatorcontrib>Mérillon, Jean‐Michel</creatorcontrib><creatorcontrib>Desmoulière, Alexis</creatorcontrib><creatorcontrib>Rosenbaum, Jean</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hepatology (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Godichaud, Sandrine</au><au>Krisa, Stéphanie</au><au>Couronné, Baptiste</au><au>Dubuisson, Liliane</au><au>Mérillon, Jean‐Michel</au><au>Desmoulière, Alexis</au><au>Rosenbaum, Jean</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deactivation of cultured human liver myofibroblasts by Trans‐resveratrol, a grapevine‐derived polyphenol</atitle><jtitle>Hepatology (Baltimore, Md.)</jtitle><addtitle>Hepatology</addtitle><date>2000-04</date><risdate>2000</risdate><volume>31</volume><issue>4</issue><spage>922</spage><epage>931</epage><pages>922-931</pages><issn>0270-9139</issn><eissn>1527-3350</eissn><coden>HPTLD9</coden><abstract>Liver myofibroblasts are major actors in the development of liver fibrosis and cancer progression. There is a large interest in drugs that might deactivate these cells. Many studies have shown that the grapevine‐derived polyphenol, trans‐resveratrol, and other stilbenes have therapeutic potential in some diseases. In this work, we have studied the effect of grapevine polyphenols on cultured human liver myofibroblasts. We have shown that trans‐resveratrol profoundly affects myofibroblast phenotype. Trans‐resveratrol induced morphological modifications. It markedly reduced proliferation of myofibroblasts in a dose‐dependent manner. Trans‐resveratrol also decreased the expression of α smooth muscle actin (α‐SMA) without affecting vimentin or β‐cytoplasmic actin expression. It decreased myofibroblast migration in a monolayer wounding assay. We also showed that trans‐resveratrol inhibited the messenger RNA (mRNA) expression of type I collagen. Finally, it decreased the secretion of matrix metalloproteinase 2 (MMP‐2). We conclude that
trans‐resveratrol can deactivate human liver myofibroblasts. In the second part of this study, we have shown that neither
trans‐piceid (a glycosylated analog) nor
trans‐piceatannol (a hydroxylated analog) reproduces
trans‐resveratrol effects on liver myofibroblasts. We finally show that, although trans‐resveratrol decreases the proliferation of skin fibroblast and vascular smooth muscle cells, it does not affect their expression of α‐SMA, which indicates some cell specificity.</abstract><cop>Philadelphia, PA</cop><pub>W.B. Saunders</pub><pmid>10733549</pmid><doi>10.1053/he.2000.5848</doi><tpages>10</tpages></addata></record> |
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| subjects | Actins - analysis Antioxidants - pharmacology Biological and medical sciences Cell Division - drug effects Cell Movement - drug effects Cells, Cultured Enzyme Activation - drug effects Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - ultrastructure Gastroenterology. Liver. Pancreas. Abdomen Gene Expression - drug effects Humans Liver - cytology Liver. Biliary tract. Portal circulation. Exocrine pancreas Matrix Metalloproteinase 2 - metabolism Medical sciences Microscopy, Electron Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases - metabolism Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Other diseases. Semiology Procollagen - genetics RNA, Messenger - analysis Stem Cells - drug effects Stem Cells - metabolism Stem Cells - ultrastructure Stilbenes - pharmacology |
| title | Deactivation of cultured human liver myofibroblasts by Trans‐resveratrol, a grapevine‐derived polyphenol |
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