CELL TYPE-DEPENDENT COLLAGEN-TYPE RECOGNITION BY CELL RECEPTORS
Affinity chromatography of a number of cell types on collagens I and III reveals three proteins with MRof 250, 170 and 140kDa. These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tiss...
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| Veröffentlicht in: | Cell biology international 2001-07, Vol.25 (7), p.643-648 |
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| creator | Sadofiev, L.A. Nikolaenko, N.S. Kalmykova, N.V. Podgornaya, O.I. |
| description | Affinity chromatography of a number of cell types on collagens I and III reveals three proteins with MRof 250, 170 and 140kDa. These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tissue origin. Polyclonal antibodies (Ab) raised against 170 and 140kDa polypeptides and used in immunofluorescence show membrane localisation for both, with their distribution being similar to each other and to the distribution of the integrin β1 chain. Abp140 and commercial monoclonal antibodies against α2chain stain a band of the same molecular mass as from purified collagen binding proteins from liver cells, indicating that the 140kDa protein is probably the α2integrin chain. The α2chain containing integrins are therefore able to discriminate collagen types I and III and collagen type recognition by this receptor is cell-type dependent. |
| doi_str_mv | 10.1006/cbir.1999.0487 |
| format | Article |
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These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tissue origin. Polyclonal antibodies (Ab) raised against 170 and 140kDa polypeptides and used in immunofluorescence show membrane localisation for both, with their distribution being similar to each other and to the distribution of the integrin β1 chain. Abp140 and commercial monoclonal antibodies against α2chain stain a band of the same molecular mass as from purified collagen binding proteins from liver cells, indicating that the 140kDa protein is probably the α2integrin chain. The α2chain containing integrins are therefore able to discriminate collagen types I and III and collagen type recognition by this receptor is cell-type dependent.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1006/cbir.1999.0487</identifier><identifier>PMID: 11448104</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Animals ; Antibodies - immunology ; Cell Line ; collagen ; Collagen - metabolism ; connective tissue ; extracellular matrix ; Fibroblasts - chemistry ; Fluorescent Antibody Technique ; Humans ; integrins ; Integrins - analysis ; Integrins - immunology ; Integrins - metabolism ; Liver - chemistry ; Osteoblasts - chemistry ; Rats ; receptors ; Receptors, Collagen ; Tumor Cells, Cultured</subject><ispartof>Cell biology international, 2001-07, Vol.25 (7), p.643-648</ispartof><rights>2001 Academic Press</rights><rights>The Author(s) Journal compilation © 2001 International Federation for Cell Biology</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1006%2Fcbir.1999.0487$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1006%2Fcbir.1999.0487$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11448104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sadofiev, L.A.</creatorcontrib><creatorcontrib>Nikolaenko, N.S.</creatorcontrib><creatorcontrib>Kalmykova, N.V.</creatorcontrib><creatorcontrib>Podgornaya, O.I.</creatorcontrib><title>CELL TYPE-DEPENDENT COLLAGEN-TYPE RECOGNITION BY CELL RECEPTORS</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>Affinity chromatography of a number of cell types on collagens I and III reveals three proteins with MRof 250, 170 and 140kDa. These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tissue origin. Polyclonal antibodies (Ab) raised against 170 and 140kDa polypeptides and used in immunofluorescence show membrane localisation for both, with their distribution being similar to each other and to the distribution of the integrin β1 chain. Abp140 and commercial monoclonal antibodies against α2chain stain a band of the same molecular mass as from purified collagen binding proteins from liver cells, indicating that the 140kDa protein is probably the α2integrin chain. The α2chain containing integrins are therefore able to discriminate collagen types I and III and collagen type recognition by this receptor is cell-type dependent.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Cell Line</subject><subject>collagen</subject><subject>Collagen - metabolism</subject><subject>connective tissue</subject><subject>extracellular matrix</subject><subject>Fibroblasts - chemistry</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>integrins</subject><subject>Integrins - analysis</subject><subject>Integrins - immunology</subject><subject>Integrins - metabolism</subject><subject>Liver - chemistry</subject><subject>Osteoblasts - chemistry</subject><subject>Rats</subject><subject>receptors</subject><subject>Receptors, Collagen</subject><subject>Tumor Cells, Cultured</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhi3Uqny01x5RTr1lsdcfsU8IgtmulCYrCCBOluNMJMMuC_YuLf--CVm1p6qnGY2e95XmQegrwROCsThxjQ8TopSaYCazPXRAsOKppJx_GHbBU6EU30eHMT5gTAiT4hPa7yeTBLMDdJrrokjq-4VOL_RClxe6rJO8KoqzmS7T4Z5c6byalfN6XpXJ-X3yHuhvelFXV9ef0cfOLiN82c0jdHOp6_x7WlSzeX5WpI4RmaWCd65RDQDtlMAULGZMCammDiDjvGu4a6dWdh2VtqWcNpa7jkvqnJuCtYweoW9j73NYv2whbszKRwfLpX2C9TaaDCvFGBc9OBlBF9YxBujMc_ArG94MwWZQZgZlZlBmBmV94HjXvG1W0P7Fd456gI3AT7-Et__Umfx8XhLCh950jPm4gV9_YjY8GpHRjJu7cmZuWSayH-rOXPa8HHnoNb56CCY6D08OWh_AbUy79v964TcDMJRN</recordid><startdate>200107</startdate><enddate>200107</enddate><creator>Sadofiev, L.A.</creator><creator>Nikolaenko, N.S.</creator><creator>Kalmykova, N.V.</creator><creator>Podgornaya, O.I.</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200107</creationdate><title>CELL TYPE-DEPENDENT COLLAGEN-TYPE RECOGNITION BY CELL RECEPTORS</title><author>Sadofiev, L.A. ; Nikolaenko, N.S. ; Kalmykova, N.V. ; Podgornaya, O.I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4187-65fcb9bee3f9603ea04496892cee755fb5cd2a8ff38ad353ba5cf583ccc2eaa43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Cell Line</topic><topic>collagen</topic><topic>Collagen - metabolism</topic><topic>connective tissue</topic><topic>extracellular matrix</topic><topic>Fibroblasts - chemistry</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>integrins</topic><topic>Integrins - analysis</topic><topic>Integrins - immunology</topic><topic>Integrins - metabolism</topic><topic>Liver - chemistry</topic><topic>Osteoblasts - chemistry</topic><topic>Rats</topic><topic>receptors</topic><topic>Receptors, Collagen</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sadofiev, L.A.</creatorcontrib><creatorcontrib>Nikolaenko, N.S.</creatorcontrib><creatorcontrib>Kalmykova, N.V.</creatorcontrib><creatorcontrib>Podgornaya, O.I.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sadofiev, L.A.</au><au>Nikolaenko, N.S.</au><au>Kalmykova, N.V.</au><au>Podgornaya, O.I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CELL TYPE-DEPENDENT COLLAGEN-TYPE RECOGNITION BY CELL RECEPTORS</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2001-07</date><risdate>2001</risdate><volume>25</volume><issue>7</issue><spage>643</spage><epage>648</epage><pages>643-648</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Affinity chromatography of a number of cell types on collagens I and III reveals three proteins with MRof 250, 170 and 140kDa. These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tissue origin. Polyclonal antibodies (Ab) raised against 170 and 140kDa polypeptides and used in immunofluorescence show membrane localisation for both, with their distribution being similar to each other and to the distribution of the integrin β1 chain. Abp140 and commercial monoclonal antibodies against α2chain stain a band of the same molecular mass as from purified collagen binding proteins from liver cells, indicating that the 140kDa protein is probably the α2integrin chain. The α2chain containing integrins are therefore able to discriminate collagen types I and III and collagen type recognition by this receptor is cell-type dependent.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>11448104</pmid><doi>10.1006/cbir.1999.0487</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cell biology international, 2001-07, Vol.25 (7), p.643-648 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antibodies - immunology Cell Line collagen Collagen - metabolism connective tissue extracellular matrix Fibroblasts - chemistry Fluorescent Antibody Technique Humans integrins Integrins - analysis Integrins - immunology Integrins - metabolism Liver - chemistry Osteoblasts - chemistry Rats receptors Receptors, Collagen Tumor Cells, Cultured |
| title | CELL TYPE-DEPENDENT COLLAGEN-TYPE RECOGNITION BY CELL RECEPTORS |
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