Expression of polymorphic msp1beta genes during acute anaplasma Marginale rickettsemia
Immunization of cattle with native MSP1 induces protection against Anaplasma marginale. The native immunogen is composed of a single MSP1a protein and multiple, undefined MSP1b polypeptides. In addition to the originally sequenced gene, designated msp1beta(F1), we identified three complete msp1beta...
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| Veröffentlicht in: | Infection and immunity 2000-04, Vol.68 (4), p.1946-1952 |
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| container_end_page | 1952 |
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| container_issue | 4 |
| container_start_page | 1946 |
| container_title | Infection and immunity |
| container_volume | 68 |
| creator | Camacho-Nuez, M de Lourdes Muñoz, M Suarez, C E McGuire, T C Brown, W C Palmer, G H |
| description | Immunization of cattle with native MSP1 induces protection against Anaplasma marginale. The native immunogen is composed of a single MSP1a protein and multiple, undefined MSP1b polypeptides. In addition to the originally sequenced gene, designated msp1beta(F1), we identified three complete msp1beta genes in the Florida strain: msp1beta(F2), msp1beta(F3), and msp1beta(F4). Each of these polymorphic genes encodes a structurally unique MSP1b protein, and unique transcripts can be identified during acute A. marginale rickettsemia. The structural polymorphism is clustered in discrete variable regions, and each MSP1b protein results from a unique mosaic of five variable regions. Although each of the MSP1b proteins in the Florida strain contains epitopes recognized by serum antibody induced by protective immunization with the native MSP1 complex, the variable regions also include epitopes expressed by some but not all of the MSP1b proteins. These data support testing recombinant vaccines composed of the multiple antigenically and structurally unique MSP1b proteins combined with MSP1a in order to mimic the efficacy of native MSP1 immunization. |
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The native immunogen is composed of a single MSP1a protein and multiple, undefined MSP1b polypeptides. In addition to the originally sequenced gene, designated msp1beta(F1), we identified three complete msp1beta genes in the Florida strain: msp1beta(F2), msp1beta(F3), and msp1beta(F4). Each of these polymorphic genes encodes a structurally unique MSP1b protein, and unique transcripts can be identified during acute A. marginale rickettsemia. The structural polymorphism is clustered in discrete variable regions, and each MSP1b protein results from a unique mosaic of five variable regions. Although each of the MSP1b proteins in the Florida strain contains epitopes recognized by serum antibody induced by protective immunization with the native MSP1 complex, the variable regions also include epitopes expressed by some but not all of the MSP1b proteins. These data support testing recombinant vaccines composed of the multiple antigenically and structurally unique MSP1b proteins combined with MSP1a in order to mimic the efficacy of native MSP1 immunization.</description><identifier>ISSN: 0019-9567</identifier><identifier>PMID: 10722587</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Amino Acids - metabolism ; Anaplasma - genetics ; Anaplasma - metabolism ; Animals ; Antibodies - blood ; Antibodies - metabolism ; B-Lymphocytes - immunology ; Bacteremia - genetics ; Bacteremia - metabolism ; Cattle ; Cloning, Molecular ; Conserved Sequence ; Epitopes ; Gene Expression ; Merozoite Surface Protein 1 - genetics ; Merozoite Surface Protein 1 - metabolism ; Models, Genetic ; Molecular Sequence Data ; Polymorphism, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Transcription, Genetic</subject><ispartof>Infection and immunity, 2000-04, Vol.68 (4), p.1946-1952</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10722587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Camacho-Nuez, M</creatorcontrib><creatorcontrib>de Lourdes Muñoz, M</creatorcontrib><creatorcontrib>Suarez, C E</creatorcontrib><creatorcontrib>McGuire, T C</creatorcontrib><creatorcontrib>Brown, W C</creatorcontrib><creatorcontrib>Palmer, G H</creatorcontrib><title>Expression of polymorphic msp1beta genes during acute anaplasma Marginale rickettsemia</title><title>Infection and immunity</title><addtitle>Infect Immun</addtitle><description>Immunization of cattle with native MSP1 induces protection against Anaplasma marginale. The native immunogen is composed of a single MSP1a protein and multiple, undefined MSP1b polypeptides. In addition to the originally sequenced gene, designated msp1beta(F1), we identified three complete msp1beta genes in the Florida strain: msp1beta(F2), msp1beta(F3), and msp1beta(F4). Each of these polymorphic genes encodes a structurally unique MSP1b protein, and unique transcripts can be identified during acute A. marginale rickettsemia. The structural polymorphism is clustered in discrete variable regions, and each MSP1b protein results from a unique mosaic of five variable regions. Although each of the MSP1b proteins in the Florida strain contains epitopes recognized by serum antibody induced by protective immunization with the native MSP1 complex, the variable regions also include epitopes expressed by some but not all of the MSP1b proteins. These data support testing recombinant vaccines composed of the multiple antigenically and structurally unique MSP1b proteins combined with MSP1a in order to mimic the efficacy of native MSP1 immunization.</description><subject>Amino Acid Sequence</subject><subject>Amino Acids - metabolism</subject><subject>Anaplasma - genetics</subject><subject>Anaplasma - metabolism</subject><subject>Animals</subject><subject>Antibodies - blood</subject><subject>Antibodies - metabolism</subject><subject>B-Lymphocytes - immunology</subject><subject>Bacteremia - genetics</subject><subject>Bacteremia - metabolism</subject><subject>Cattle</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>Epitopes</subject><subject>Gene Expression</subject><subject>Merozoite Surface Protein 1 - genetics</subject><subject>Merozoite Surface Protein 1 - metabolism</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Polymorphism, Genetic</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic</subject><issn>0019-9567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kD1PwzAURT2AaCn8BeSJLZJjO7E9oqp8SEUsFWv0nDwHQ5wYO5Hov6cSZbq60jl3uBdkzVhpClPVakWuc_48VSmlviKrkinOK63W5H33ExPm7KeRTo7GaTiGKcUP39KQY2lxBtrjiJl2S_JjT6FdZqQwQhwgB6CvkHo_woA0-fYL5zlj8HBDLh0MGW_PuSGHx91h-1zs355etg_7IlZSFbZS3NROdkJyZTnUQlrUXNQOWa3BgrNgeCvAdU47LbHUTpXK2JNSKUSxIfd_szFN3wvmuQk-tzgMMOK05EYxYxhT8gTencHFBuyamHyAdGz-jxC_zkFa5w</recordid><startdate>200004</startdate><enddate>200004</enddate><creator>Camacho-Nuez, M</creator><creator>de Lourdes Muñoz, M</creator><creator>Suarez, C E</creator><creator>McGuire, T C</creator><creator>Brown, W C</creator><creator>Palmer, G H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200004</creationdate><title>Expression of polymorphic msp1beta genes during acute anaplasma Marginale rickettsemia</title><author>Camacho-Nuez, M ; de Lourdes Muñoz, M ; Suarez, C E ; McGuire, T C ; Brown, W C ; Palmer, G H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p547-b57296f4d3427b2a634be8236fe068abafba92c3afdf8f84e18f7179bf4d57ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acids - metabolism</topic><topic>Anaplasma - genetics</topic><topic>Anaplasma - metabolism</topic><topic>Animals</topic><topic>Antibodies - blood</topic><topic>Antibodies - metabolism</topic><topic>B-Lymphocytes - immunology</topic><topic>Bacteremia - genetics</topic><topic>Bacteremia - metabolism</topic><topic>Cattle</topic><topic>Cloning, Molecular</topic><topic>Conserved Sequence</topic><topic>Epitopes</topic><topic>Gene Expression</topic><topic>Merozoite Surface Protein 1 - genetics</topic><topic>Merozoite Surface Protein 1 - metabolism</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Polymorphism, Genetic</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Camacho-Nuez, M</creatorcontrib><creatorcontrib>de Lourdes Muñoz, M</creatorcontrib><creatorcontrib>Suarez, C E</creatorcontrib><creatorcontrib>McGuire, T C</creatorcontrib><creatorcontrib>Brown, W C</creatorcontrib><creatorcontrib>Palmer, G H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Infection and immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Camacho-Nuez, M</au><au>de Lourdes Muñoz, M</au><au>Suarez, C E</au><au>McGuire, T C</au><au>Brown, W C</au><au>Palmer, G H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of polymorphic msp1beta genes during acute anaplasma Marginale rickettsemia</atitle><jtitle>Infection and immunity</jtitle><addtitle>Infect Immun</addtitle><date>2000-04</date><risdate>2000</risdate><volume>68</volume><issue>4</issue><spage>1946</spage><epage>1952</epage><pages>1946-1952</pages><issn>0019-9567</issn><abstract>Immunization of cattle with native MSP1 induces protection against Anaplasma marginale. The native immunogen is composed of a single MSP1a protein and multiple, undefined MSP1b polypeptides. In addition to the originally sequenced gene, designated msp1beta(F1), we identified three complete msp1beta genes in the Florida strain: msp1beta(F2), msp1beta(F3), and msp1beta(F4). Each of these polymorphic genes encodes a structurally unique MSP1b protein, and unique transcripts can be identified during acute A. marginale rickettsemia. The structural polymorphism is clustered in discrete variable regions, and each MSP1b protein results from a unique mosaic of five variable regions. Although each of the MSP1b proteins in the Florida strain contains epitopes recognized by serum antibody induced by protective immunization with the native MSP1 complex, the variable regions also include epitopes expressed by some but not all of the MSP1b proteins. These data support testing recombinant vaccines composed of the multiple antigenically and structurally unique MSP1b proteins combined with MSP1a in order to mimic the efficacy of native MSP1 immunization.</abstract><cop>United States</cop><pmid>10722587</pmid><tpages>7</tpages></addata></record> |
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| ispartof | Infection and immunity, 2000-04, Vol.68 (4), p.1946-1952 |
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| language | eng |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Amino Acid Sequence Amino Acids - metabolism Anaplasma - genetics Anaplasma - metabolism Animals Antibodies - blood Antibodies - metabolism B-Lymphocytes - immunology Bacteremia - genetics Bacteremia - metabolism Cattle Cloning, Molecular Conserved Sequence Epitopes Gene Expression Merozoite Surface Protein 1 - genetics Merozoite Surface Protein 1 - metabolism Models, Genetic Molecular Sequence Data Polymorphism, Genetic Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Transcription, Genetic |
| title | Expression of polymorphic msp1beta genes during acute anaplasma Marginale rickettsemia |
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