Absence of lipophosphoglycan-like glycoconjugates in Entamoeba dispar
Invasive amoebiasis is the result of infection of Entamoeba histolytica. The closely related Entamoeba dispar can colonize the human gut but does not cause invasive disease. In this study, E. dispar was analysed for the presence of the lipophosphoglycan-like (LPG) glycoconjugate known to be present...
Gespeichert in:
| Veröffentlicht in: | Parasitology 2000-01, Vol.120 (1), p.31-35 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 35 |
|---|---|
| container_issue | 1 |
| container_start_page | 31 |
| container_title | Parasitology |
| container_volume | 120 |
| creator | BHATTACHARYA, A. ARYA, R. CLARK, C. G. ACKERS, J. P. |
| description | Invasive amoebiasis is the result of infection of Entamoeba histolytica. The closely related Entamoeba dispar can colonize
the human gut but does not cause invasive disease. In this study, E. dispar was analysed for the presence of the
lipophosphoglycan-like (LPG) glycoconjugate known to be present on the cell surface of E. histolytica. E. dispar cells were
radio-isotope labelled with [3H]galactose or [3H]inositol. The acidic glycoconjugates were extracted and analysed by
hydrophobic chromatography over phenyl–Sepharose and by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
No LPG-like molecules could be identified in E. dispar in contrast to E. histolytica, suggesting that these molecules may
be absent in the non-pathogenic species. |
| doi_str_mv | 10.1017/S0031182099005259 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70990046</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cupid>10_1017_S0031182099005259</cupid><sourcerecordid>70990046</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-8fc9f2aede108f0971d526294c028cbd420c7fa2ad5d0544604a761420ef3c673</originalsourceid><addsrcrecordid>eNqFkEFv1DAQha0K1G4LP6AXlAPqLTB2Yjs-VtV2KaqEgMLVmjj24m0SBzuR2n9P0l0VJCQ4WGP5fTN-8wg5p_COApXvvwIUlFYMlALgjKsjsqKlUHlFBX1BVoucL_oJOU1pBwCiEOyYnFCQTDBRrMj6sk62NzYLLmv9EIYfIc1n2z4a7PPW39tsuQcT-t20xdGmzPfZuh-xC7bGrPFpwPiKvHTYJvv6UM_It-v13dWH_PbT5ubq8jY3ZaHGvHJGOYa2sRQqB0rShs8-VGmAVaZuSgZGOmTY8AZ4WQooUQo6P1tXGCGLM3KxnzvE8HOyadSdT8a2LfY2TEnLpyRK8V-QSs65FGwG6R40MaQUrdND9B3GR01BLyHrv0Kee94chk91Z5s_OvapzsDbA4DJYOsi9san3xyTjLEFy_eYT6N9eJYx3ut5V8m12HzW7Mv3zeajVHr5tzh4xa6OvtlavQtT7OfE_-H2FznwoTQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17555762</pqid></control><display><type>article</type><title>Absence of lipophosphoglycan-like glycoconjugates in Entamoeba dispar</title><source>MEDLINE</source><source>Cambridge Journals</source><creator>BHATTACHARYA, A. ; ARYA, R. ; CLARK, C. G. ; ACKERS, J. P.</creator><creatorcontrib>BHATTACHARYA, A. ; ARYA, R. ; CLARK, C. G. ; ACKERS, J. P.</creatorcontrib><description>Invasive amoebiasis is the result of infection of Entamoeba histolytica. The closely related Entamoeba dispar can colonize
the human gut but does not cause invasive disease. In this study, E. dispar was analysed for the presence of the
lipophosphoglycan-like (LPG) glycoconjugate known to be present on the cell surface of E. histolytica. E. dispar cells were
radio-isotope labelled with [3H]galactose or [3H]inositol. The acidic glycoconjugates were extracted and analysed by
hydrophobic chromatography over phenyl–Sepharose and by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
No LPG-like molecules could be identified in E. dispar in contrast to E. histolytica, suggesting that these molecules may
be absent in the non-pathogenic species.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182099005259</identifier><identifier>PMID: 10726263</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge: Cambridge University Press</publisher><subject>Animals ; Biological and medical sciences ; cell surface ; Chromatography, Agarose ; Electrophoresis, Polyacrylamide Gel ; Entamoeba - chemistry ; Entamoeba - pathogenicity ; Entamoeba dispar ; Entamoeba histolytica ; Entamoeba histolytica - chemistry ; Entamoeba histolytica - pathogenicity ; Entamoebiasis - parasitology ; Fundamental and applied biological sciences. Psychology ; glycoconjugate ; glycoconjugates ; Glycosphingolipids - chemistry ; Glycosphingolipids - isolation & purification ; Life cycle. Host-agent relationship. Pathogenesis ; lipophosphoglycan ; lipophosphoglycans ; Protozoa ; Tritium - chemistry</subject><ispartof>Parasitology, 2000-01, Vol.120 (1), p.31-35</ispartof><rights>2000 Cambridge University Press</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-8fc9f2aede108f0971d526294c028cbd420c7fa2ad5d0544604a761420ef3c673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182099005259/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,4009,27902,27903,27904,55606</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1272223$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10726263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BHATTACHARYA, A.</creatorcontrib><creatorcontrib>ARYA, R.</creatorcontrib><creatorcontrib>CLARK, C. G.</creatorcontrib><creatorcontrib>ACKERS, J. P.</creatorcontrib><title>Absence of lipophosphoglycan-like glycoconjugates in Entamoeba dispar</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Invasive amoebiasis is the result of infection of Entamoeba histolytica. The closely related Entamoeba dispar can colonize
the human gut but does not cause invasive disease. In this study, E. dispar was analysed for the presence of the
lipophosphoglycan-like (LPG) glycoconjugate known to be present on the cell surface of E. histolytica. E. dispar cells were
radio-isotope labelled with [3H]galactose or [3H]inositol. The acidic glycoconjugates were extracted and analysed by
hydrophobic chromatography over phenyl–Sepharose and by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
No LPG-like molecules could be identified in E. dispar in contrast to E. histolytica, suggesting that these molecules may
be absent in the non-pathogenic species.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>cell surface</subject><subject>Chromatography, Agarose</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Entamoeba - chemistry</subject><subject>Entamoeba - pathogenicity</subject><subject>Entamoeba dispar</subject><subject>Entamoeba histolytica</subject><subject>Entamoeba histolytica - chemistry</subject><subject>Entamoeba histolytica - pathogenicity</subject><subject>Entamoebiasis - parasitology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycoconjugate</subject><subject>glycoconjugates</subject><subject>Glycosphingolipids - chemistry</subject><subject>Glycosphingolipids - isolation & purification</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>lipophosphoglycan</subject><subject>lipophosphoglycans</subject><subject>Protozoa</subject><subject>Tritium - chemistry</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv1DAQha0K1G4LP6AXlAPqLTB2Yjs-VtV2KaqEgMLVmjj24m0SBzuR2n9P0l0VJCQ4WGP5fTN-8wg5p_COApXvvwIUlFYMlALgjKsjsqKlUHlFBX1BVoucL_oJOU1pBwCiEOyYnFCQTDBRrMj6sk62NzYLLmv9EIYfIc1n2z4a7PPW39tsuQcT-t20xdGmzPfZuh-xC7bGrPFpwPiKvHTYJvv6UM_It-v13dWH_PbT5ubq8jY3ZaHGvHJGOYa2sRQqB0rShs8-VGmAVaZuSgZGOmTY8AZ4WQooUQo6P1tXGCGLM3KxnzvE8HOyadSdT8a2LfY2TEnLpyRK8V-QSs65FGwG6R40MaQUrdND9B3GR01BLyHrv0Kee94chk91Z5s_OvapzsDbA4DJYOsi9san3xyTjLEFy_eYT6N9eJYx3ut5V8m12HzW7Mv3zeajVHr5tzh4xa6OvtlavQtT7OfE_-H2FznwoTQ</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>BHATTACHARYA, A.</creator><creator>ARYA, R.</creator><creator>CLARK, C. G.</creator><creator>ACKERS, J. P.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Absence of lipophosphoglycan-like glycoconjugates in Entamoeba dispar</title><author>BHATTACHARYA, A. ; ARYA, R. ; CLARK, C. G. ; ACKERS, J. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-8fc9f2aede108f0971d526294c028cbd420c7fa2ad5d0544604a761420ef3c673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>cell surface</topic><topic>Chromatography, Agarose</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Entamoeba - chemistry</topic><topic>Entamoeba - pathogenicity</topic><topic>Entamoeba dispar</topic><topic>Entamoeba histolytica</topic><topic>Entamoeba histolytica - chemistry</topic><topic>Entamoeba histolytica - pathogenicity</topic><topic>Entamoebiasis - parasitology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glycoconjugate</topic><topic>glycoconjugates</topic><topic>Glycosphingolipids - chemistry</topic><topic>Glycosphingolipids - isolation & purification</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>lipophosphoglycan</topic><topic>lipophosphoglycans</topic><topic>Protozoa</topic><topic>Tritium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BHATTACHARYA, A.</creatorcontrib><creatorcontrib>ARYA, R.</creatorcontrib><creatorcontrib>CLARK, C. G.</creatorcontrib><creatorcontrib>ACKERS, J. P.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BHATTACHARYA, A.</au><au>ARYA, R.</au><au>CLARK, C. G.</au><au>ACKERS, J. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Absence of lipophosphoglycan-like glycoconjugates in Entamoeba dispar</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2000-01</date><risdate>2000</risdate><volume>120</volume><issue>1</issue><spage>31</spage><epage>35</epage><pages>31-35</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Invasive amoebiasis is the result of infection of Entamoeba histolytica. The closely related Entamoeba dispar can colonize
the human gut but does not cause invasive disease. In this study, E. dispar was analysed for the presence of the
lipophosphoglycan-like (LPG) glycoconjugate known to be present on the cell surface of E. histolytica. E. dispar cells were
radio-isotope labelled with [3H]galactose or [3H]inositol. The acidic glycoconjugates were extracted and analysed by
hydrophobic chromatography over phenyl–Sepharose and by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
No LPG-like molecules could be identified in E. dispar in contrast to E. histolytica, suggesting that these molecules may
be absent in the non-pathogenic species.</abstract><cop>Cambridge</cop><pub>Cambridge University Press</pub><pmid>10726263</pmid><doi>10.1017/S0031182099005259</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-1820 |
| ispartof | Parasitology, 2000-01, Vol.120 (1), p.31-35 |
| issn | 0031-1820 1469-8161 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70990046 |
| source | MEDLINE; Cambridge Journals |
| subjects | Animals Biological and medical sciences cell surface Chromatography, Agarose Electrophoresis, Polyacrylamide Gel Entamoeba - chemistry Entamoeba - pathogenicity Entamoeba dispar Entamoeba histolytica Entamoeba histolytica - chemistry Entamoeba histolytica - pathogenicity Entamoebiasis - parasitology Fundamental and applied biological sciences. Psychology glycoconjugate glycoconjugates Glycosphingolipids - chemistry Glycosphingolipids - isolation & purification Life cycle. Host-agent relationship. Pathogenesis lipophosphoglycan lipophosphoglycans Protozoa Tritium - chemistry |
| title | Absence of lipophosphoglycan-like glycoconjugates in Entamoeba dispar |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T12%3A35%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Absence%20of%20lipophosphoglycan-like%20glycoconjugates%20in%20Entamoeba%20dispar&rft.jtitle=Parasitology&rft.au=BHATTACHARYA,%20A.&rft.date=2000-01&rft.volume=120&rft.issue=1&rft.spage=31&rft.epage=35&rft.pages=31-35&rft.issn=0031-1820&rft.eissn=1469-8161&rft.coden=PARAAE&rft_id=info:doi/10.1017/S0031182099005259&rft_dat=%3Cproquest_cross%3E70990046%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17555762&rft_id=info:pmid/10726263&rft_cupid=10_1017_S0031182099005259&rfr_iscdi=true |