Establishment and Epitope Analysis of Allo-Specific Cytotoxic T Lymphocytes at a Tumor Site Recognizing a Spouse's HLA-A0206 Molecule
PROBLEM: The molecular basis of allo‐reactivity in reproductive immunity has not been fully clarified. METHOD OF STUDY: Cytotoxic T lymphocytes (CTLs) were established from tumor‐infiltrating lymphocytes (TILs). The allo‐reactivity of the CTLs against various tumor cell lines or human leukocyte ant...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2000-03, Vol.43 (3), p.167-173 |
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| container_title | American journal of reproductive immunology (1989) |
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| creator | SEKI, NAOKO YAMADA, AKIRA SUEFUJI, YUICHI MINE, TAKASHI TANAKA, SHOKO GOMI, SHIN-YA KAWAGOE, NOBUTOSHI KOUFUJI, KIKUO ITOH, KYOGO |
| description | PROBLEM: The molecular basis of allo‐reactivity in reproductive immunity has not been fully clarified.
METHOD OF STUDY: Cytotoxic T lymphocytes (CTLs) were established from tumor‐infiltrating lymphocytes (TILs). The allo‐reactivity of the CTLs against various tumor cell lines or human leukocyte antigen (HLA)‐A allele‐transfected COS‐7 cells was measured by Cr‐release or interferon‐Γ production assay.
RESULTS AND CONCLUSIONS: We have established CTLs reacting to an HLA‐A0206 molecule that matched a spouse's HLA‐A allele from the TILs of a 68‐year‐old multiparous patient with gastric cancer. The amino acids at positions 66 and 88 in the α1 domain of HLA‐A0206, both of which were common in the other HLA‐A2 subtypes, were involved in the recognition by the CTLs. Endogenous peptides in the groove were not involved in the recognition. These results suggest the presence of long‐lasting memory CTLs raised by the reproduction process, and may facilitate a better understanding of the molecular basis of allo‐recognition during reproduction. |
| doi_str_mv | 10.1111/j.8755-8920.2000.430306.x |
| format | Article |
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METHOD OF STUDY: Cytotoxic T lymphocytes (CTLs) were established from tumor‐infiltrating lymphocytes (TILs). The allo‐reactivity of the CTLs against various tumor cell lines or human leukocyte antigen (HLA)‐A allele‐transfected COS‐7 cells was measured by Cr‐release or interferon‐Γ production assay.
RESULTS AND CONCLUSIONS: We have established CTLs reacting to an HLA‐A0206 molecule that matched a spouse's HLA‐A allele from the TILs of a 68‐year‐old multiparous patient with gastric cancer. The amino acids at positions 66 and 88 in the α1 domain of HLA‐A0206, both of which were common in the other HLA‐A2 subtypes, were involved in the recognition by the CTLs. Endogenous peptides in the groove were not involved in the recognition. These results suggest the presence of long‐lasting memory CTLs raised by the reproduction process, and may facilitate a better understanding of the molecular basis of allo‐recognition during reproduction.</description><identifier>ISSN: 1046-7408</identifier><identifier>ISSN: 8755-8920</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.8755-8920.2000.430306.x</identifier><identifier>PMID: 10735593</identifier><language>eng</language><publisher>Copenhagen: Munksgaard</publisher><subject>Aged ; Alleles ; Allo-reactive CTL ; Analysis of the immune response. Humoral and cellular immunity ; Animals ; Biological and medical sciences ; COS Cells ; cytotoxic T lymphocytes ; Epitopes, T-Lymphocyte - analysis ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; histocompatibility antigen HLA ; HLA-A2 ; HLA-A2 Antigen - genetics ; HLA-A2 Antigen - immunology ; HLA-A2 Antigen - metabolism ; Humans ; Immunobiology ; Immunologic Memory ; Lymphocytes, Tumor-Infiltrating - immunology ; Lymphokines, interleukins ( function, expression) ; Male ; memory T cells ; pregnancy ; Regulatory factors and their cellular receptors ; Spouses ; Stomach Neoplasms - immunology ; T-Lymphocytes, Cytotoxic - immunology ; T-Lymphocytes, Cytotoxic - metabolism ; Transfection ; Tumor Cells, Cultured</subject><ispartof>American journal of reproductive immunology (1989), 2000-03, Vol.43 (3), p.167-173</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.8755-8920.2000.430306.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.8755-8920.2000.430306.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1332957$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10735593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEKI, NAOKO</creatorcontrib><creatorcontrib>YAMADA, AKIRA</creatorcontrib><creatorcontrib>SUEFUJI, YUICHI</creatorcontrib><creatorcontrib>MINE, TAKASHI</creatorcontrib><creatorcontrib>TANAKA, SHOKO</creatorcontrib><creatorcontrib>GOMI, SHIN-YA</creatorcontrib><creatorcontrib>KAWAGOE, NOBUTOSHI</creatorcontrib><creatorcontrib>KOUFUJI, KIKUO</creatorcontrib><creatorcontrib>ITOH, KYOGO</creatorcontrib><title>Establishment and Epitope Analysis of Allo-Specific Cytotoxic T Lymphocytes at a Tumor Site Recognizing a Spouse's HLA-A0206 Molecule</title><title>American journal of reproductive immunology (1989)</title><addtitle>American Journal of Reproductive Immunology</addtitle><description>PROBLEM: The molecular basis of allo‐reactivity in reproductive immunity has not been fully clarified.
METHOD OF STUDY: Cytotoxic T lymphocytes (CTLs) were established from tumor‐infiltrating lymphocytes (TILs). The allo‐reactivity of the CTLs against various tumor cell lines or human leukocyte antigen (HLA)‐A allele‐transfected COS‐7 cells was measured by Cr‐release or interferon‐Γ production assay.
RESULTS AND CONCLUSIONS: We have established CTLs reacting to an HLA‐A0206 molecule that matched a spouse's HLA‐A allele from the TILs of a 68‐year‐old multiparous patient with gastric cancer. The amino acids at positions 66 and 88 in the α1 domain of HLA‐A0206, both of which were common in the other HLA‐A2 subtypes, were involved in the recognition by the CTLs. Endogenous peptides in the groove were not involved in the recognition. These results suggest the presence of long‐lasting memory CTLs raised by the reproduction process, and may facilitate a better understanding of the molecular basis of allo‐recognition during reproduction.</description><subject>Aged</subject><subject>Alleles</subject><subject>Allo-reactive CTL</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>COS Cells</subject><subject>cytotoxic T lymphocytes</subject><subject>Epitopes, T-Lymphocyte - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>histocompatibility antigen HLA</subject><subject>HLA-A2</subject><subject>HLA-A2 Antigen - genetics</subject><subject>HLA-A2 Antigen - immunology</subject><subject>HLA-A2 Antigen - metabolism</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Immunologic Memory</subject><subject>Lymphocytes, Tumor-Infiltrating - immunology</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Male</subject><subject>memory T cells</subject><subject>pregnancy</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Spouses</subject><subject>Stomach Neoplasms - immunology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>T-Lymphocytes, Cytotoxic - metabolism</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>1046-7408</issn><issn>8755-8920</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P0zAQxSMEYv_AV0BGQnBKmNiJHd-IqtJdKLCiFRwt13V2XZw4xIm24c73xlEq4Aa--En-zfPMvCh6nkKShvP6kBQsz-OCY0gwACQZAQI0OT6IzlMKEEPB2cOgIaMxy6A4iy68PwSy4IQ9js5SYCTPOTmPfi59L3fW-LtaNz2SzR4tW9O7VqOykXb0xiNXodJaF29arUxlFFqMvevdMagtWo91e-fU2GuPZDBA26F2HdqYXqPPWrnbxvwwzW142LRu8PqVR1frMi4BA0UfnNVqsPpJ9KiS1uunp_sy2r5dbhdX8frT6npRrmOVpZjGe4wJ5lXGoKLZnlOsOJOTVMB2kijF9lzqnEiVpxXXO8o5V2HWoqBE4YJcRi9n27Zz3wfte1Ebr7S1stGhN8GAF0WGs3-CKcsB0wwCyGdQdc77Tlei7Uwtu1GkIKasxEFMWYkpKzFlJeasxDHUPjt9Muxqvf-rcg4nAC9OgPRK2qqTjTL-D0fCMnIWsDczdm-sHv-_AVG-u551sIhnC-N7ffxtIbtvgjLCcvH140q8p4vVTf4FxA35BfdYvkg</recordid><startdate>200003</startdate><enddate>200003</enddate><creator>SEKI, NAOKO</creator><creator>YAMADA, AKIRA</creator><creator>SUEFUJI, YUICHI</creator><creator>MINE, TAKASHI</creator><creator>TANAKA, SHOKO</creator><creator>GOMI, SHIN-YA</creator><creator>KAWAGOE, NOBUTOSHI</creator><creator>KOUFUJI, KIKUO</creator><creator>ITOH, KYOGO</creator><general>Munksgaard</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200003</creationdate><title>Establishment and Epitope Analysis of Allo-Specific Cytotoxic T Lymphocytes at a Tumor Site Recognizing a Spouse's HLA-A0206 Molecule</title><author>SEKI, NAOKO ; YAMADA, AKIRA ; SUEFUJI, YUICHI ; MINE, TAKASHI ; TANAKA, SHOKO ; GOMI, SHIN-YA ; KAWAGOE, NOBUTOSHI ; KOUFUJI, KIKUO ; ITOH, KYOGO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4126-d22329f470f64d962c97af64dc07ba3cc7d9ae53ac51f9eb6999c1078863c283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Aged</topic><topic>Alleles</topic><topic>Allo-reactive CTL</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>COS Cells</topic><topic>cytotoxic T lymphocytes</topic><topic>Epitopes, T-Lymphocyte - analysis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>histocompatibility antigen HLA</topic><topic>HLA-A2</topic><topic>HLA-A2 Antigen - genetics</topic><topic>HLA-A2 Antigen - immunology</topic><topic>HLA-A2 Antigen - metabolism</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Immunologic Memory</topic><topic>Lymphocytes, Tumor-Infiltrating - immunology</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Male</topic><topic>memory T cells</topic><topic>pregnancy</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Spouses</topic><topic>Stomach Neoplasms - immunology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>T-Lymphocytes, Cytotoxic - metabolism</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SEKI, NAOKO</creatorcontrib><creatorcontrib>YAMADA, AKIRA</creatorcontrib><creatorcontrib>SUEFUJI, YUICHI</creatorcontrib><creatorcontrib>MINE, TAKASHI</creatorcontrib><creatorcontrib>TANAKA, SHOKO</creatorcontrib><creatorcontrib>GOMI, SHIN-YA</creatorcontrib><creatorcontrib>KAWAGOE, NOBUTOSHI</creatorcontrib><creatorcontrib>KOUFUJI, KIKUO</creatorcontrib><creatorcontrib>ITOH, KYOGO</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEKI, NAOKO</au><au>YAMADA, AKIRA</au><au>SUEFUJI, YUICHI</au><au>MINE, TAKASHI</au><au>TANAKA, SHOKO</au><au>GOMI, SHIN-YA</au><au>KAWAGOE, NOBUTOSHI</au><au>KOUFUJI, KIKUO</au><au>ITOH, KYOGO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and Epitope Analysis of Allo-Specific Cytotoxic T Lymphocytes at a Tumor Site Recognizing a Spouse's HLA-A0206 Molecule</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>American Journal of Reproductive Immunology</addtitle><date>2000-03</date><risdate>2000</risdate><volume>43</volume><issue>3</issue><spage>167</spage><epage>173</epage><pages>167-173</pages><issn>1046-7408</issn><issn>8755-8920</issn><eissn>1600-0897</eissn><abstract>PROBLEM: The molecular basis of allo‐reactivity in reproductive immunity has not been fully clarified.
METHOD OF STUDY: Cytotoxic T lymphocytes (CTLs) were established from tumor‐infiltrating lymphocytes (TILs). The allo‐reactivity of the CTLs against various tumor cell lines or human leukocyte antigen (HLA)‐A allele‐transfected COS‐7 cells was measured by Cr‐release or interferon‐Γ production assay.
RESULTS AND CONCLUSIONS: We have established CTLs reacting to an HLA‐A0206 molecule that matched a spouse's HLA‐A allele from the TILs of a 68‐year‐old multiparous patient with gastric cancer. The amino acids at positions 66 and 88 in the α1 domain of HLA‐A0206, both of which were common in the other HLA‐A2 subtypes, were involved in the recognition by the CTLs. Endogenous peptides in the groove were not involved in the recognition. These results suggest the presence of long‐lasting memory CTLs raised by the reproduction process, and may facilitate a better understanding of the molecular basis of allo‐recognition during reproduction.</abstract><cop>Copenhagen</cop><pub>Munksgaard</pub><pmid>10735593</pmid><doi>10.1111/j.8755-8920.2000.430306.x</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2000-03, Vol.43 (3), p.167-173 |
| issn | 1046-7408 8755-8920 1600-0897 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aged Alleles Allo-reactive CTL Analysis of the immune response. Humoral and cellular immunity Animals Biological and medical sciences COS Cells cytotoxic T lymphocytes Epitopes, T-Lymphocyte - analysis Female Fundamental and applied biological sciences. Psychology Fundamental immunology histocompatibility antigen HLA HLA-A2 HLA-A2 Antigen - genetics HLA-A2 Antigen - immunology HLA-A2 Antigen - metabolism Humans Immunobiology Immunologic Memory Lymphocytes, Tumor-Infiltrating - immunology Lymphokines, interleukins ( function, expression) Male memory T cells pregnancy Regulatory factors and their cellular receptors Spouses Stomach Neoplasms - immunology T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Cytotoxic - metabolism Transfection Tumor Cells, Cultured |
| title | Establishment and Epitope Analysis of Allo-Specific Cytotoxic T Lymphocytes at a Tumor Site Recognizing a Spouse's HLA-A0206 Molecule |
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