Acrylamide Quenching of apo- and holo-α-Lactalbumin in Guanidine Hydrochloride

We have examined the fluorescence properties and acrylamide quenching of calcium-loaded (holo) and calcium-depleted (apo) α-lactalbumin (α-LA) as a function of guanidine hydrochloride (GDN/HCl) concentration. The spectral changes accompanying increasing GDN/HCl are consistent with protein unfolding...

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Veröffentlicht in:	Biochemical and biophysical research communications 2000-03, Vol.269 (3), p.709-712
Hauptverfasser:	France, Richard M., Grossman, Steven H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylamide Animals Apoproteins - chemistry Cattle Guanidine Kinetics Lactalbumin - chemistry Protein Denaturation Protein Folding Protein Structure, Tertiary Spectrometry, Fluorescence Tryptophan
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