Expression of cytokines and factors modulating apoptosis by human sinusoidal leucocytes
Liver sinusoids contain a large population of spontaneously cytotoxic cells (NK cells), CD8+ T cells and macrophages. The physiological role of these leucocytes remains unclear. They may participate in immune surveillance and peripheral tolerance by deleting tumour cells, virus-infected cells and ac...
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| Veröffentlicht in: | Journal of hepatology 2000-03, Vol.32 (3), p.392-398 |
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| creator | JONSSON, J. R EDWARDS-SMITH, C. J CATANIA, S. C MOROTOMI, Y HOGAN, P. G CLOUSTON, A. D BANSAL, A. S LYNCH, S. V STRONG, R. W POWELL, E. E |
| description | Liver sinusoids contain a large population of spontaneously cytotoxic cells (NK cells), CD8+ T cells and macrophages. The physiological role of these leucocytes remains unclear. They may participate in immune surveillance and peripheral tolerance by deleting tumour cells, virus-infected cells and activated T cells as they traffic through the liver. In order to gain further information about the function of these leucocytes within the hepatic sinusoids, we examined their production of immunomodulatory cytokines and apoptosis-related molecules.
Semi-quantitative polymerase chain reaction and immunohistochemistry were used to determine the spontaneous production of cytokines and apoptosis-related molecules by sinusoidal leucocytes isolated from donor liver preservation solution.
In comparison with matched peripheral blood mononuclear cells, sinusoidal leucocytes produced more mRNA for IL-10, IL-15, TNF-alpha, IL-18, IFN-gamma, FasL, perforin and granzyme. IL-4 and IL-12 were not detected and IL-2 was only faintly detected in the liver-derived CD4+ population. Less bcl-2 was expressed in liver-derived CD4+ and CD8+ cells in comparison with matched peripheral blood cell populations.
The cytokines produced spontaneously by sinusoidal leucocytes are consistent with their high level of activation and spontaneous cytotoxicity. Their strong expression of apoptosis-mediating molecules (FasL, perforin, granzyme and TNF-alpha) support a role for these cells in immune surveillance and peripheral tolerance induction. |
| doi_str_mv | 10.1016/S0168-8278(00)80388-8 |
| format | Article |
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Semi-quantitative polymerase chain reaction and immunohistochemistry were used to determine the spontaneous production of cytokines and apoptosis-related molecules by sinusoidal leucocytes isolated from donor liver preservation solution.
In comparison with matched peripheral blood mononuclear cells, sinusoidal leucocytes produced more mRNA for IL-10, IL-15, TNF-alpha, IL-18, IFN-gamma, FasL, perforin and granzyme. IL-4 and IL-12 were not detected and IL-2 was only faintly detected in the liver-derived CD4+ population. Less bcl-2 was expressed in liver-derived CD4+ and CD8+ cells in comparison with matched peripheral blood cell populations.
The cytokines produced spontaneously by sinusoidal leucocytes are consistent with their high level of activation and spontaneous cytotoxicity. Their strong expression of apoptosis-mediating molecules (FasL, perforin, granzyme and TNF-alpha) support a role for these cells in immune surveillance and peripheral tolerance induction.</description><identifier>ISSN: 0168-8278</identifier><identifier>EISSN: 1600-0641</identifier><identifier>DOI: 10.1016/S0168-8278(00)80388-8</identifier><identifier>PMID: 10735607</identifier><identifier>CODEN: JOHEEC</identifier><language>eng</language><publisher>Oxford: Elsevier</publisher><subject>Adjuvants, Immunologic - metabolism ; Apoptosis - physiology ; Biological and medical sciences ; Cytokines - genetics ; Cytokines - metabolism ; Digestive system ; Fas Ligand Protein ; Granzymes ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Leukocytes - metabolism ; Liver - cytology ; Liver - metabolism ; Medical sciences ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Perforin ; Pore Forming Cytotoxic Proteins ; RNA, Messenger - metabolism ; Serine Endopeptidases - genetics ; Serine Endopeptidases - metabolism ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Journal of hepatology, 2000-03, Vol.32 (3), p.392-398</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-ecc17577b9335e2bbb084a9256585b040be55730af9a2ad3e999ec5e7e0770393</citedby><cites>FETCH-LOGICAL-c334t-ecc17577b9335e2bbb084a9256585b040be55730af9a2ad3e999ec5e7e0770393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1270879$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10735607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JONSSON, J. R</creatorcontrib><creatorcontrib>EDWARDS-SMITH, C. J</creatorcontrib><creatorcontrib>CATANIA, S. C</creatorcontrib><creatorcontrib>MOROTOMI, Y</creatorcontrib><creatorcontrib>HOGAN, P. G</creatorcontrib><creatorcontrib>CLOUSTON, A. D</creatorcontrib><creatorcontrib>BANSAL, A. S</creatorcontrib><creatorcontrib>LYNCH, S. V</creatorcontrib><creatorcontrib>STRONG, R. W</creatorcontrib><creatorcontrib>POWELL, E. E</creatorcontrib><title>Expression of cytokines and factors modulating apoptosis by human sinusoidal leucocytes</title><title>Journal of hepatology</title><addtitle>J Hepatol</addtitle><description>Liver sinusoids contain a large population of spontaneously cytotoxic cells (NK cells), CD8+ T cells and macrophages. The physiological role of these leucocytes remains unclear. They may participate in immune surveillance and peripheral tolerance by deleting tumour cells, virus-infected cells and activated T cells as they traffic through the liver. In order to gain further information about the function of these leucocytes within the hepatic sinusoids, we examined their production of immunomodulatory cytokines and apoptosis-related molecules.
Semi-quantitative polymerase chain reaction and immunohistochemistry were used to determine the spontaneous production of cytokines and apoptosis-related molecules by sinusoidal leucocytes isolated from donor liver preservation solution.
In comparison with matched peripheral blood mononuclear cells, sinusoidal leucocytes produced more mRNA for IL-10, IL-15, TNF-alpha, IL-18, IFN-gamma, FasL, perforin and granzyme. IL-4 and IL-12 were not detected and IL-2 was only faintly detected in the liver-derived CD4+ population. Less bcl-2 was expressed in liver-derived CD4+ and CD8+ cells in comparison with matched peripheral blood cell populations.
The cytokines produced spontaneously by sinusoidal leucocytes are consistent with their high level of activation and spontaneous cytotoxicity. Their strong expression of apoptosis-mediating molecules (FasL, perforin, granzyme and TNF-alpha) support a role for these cells in immune surveillance and peripheral tolerance induction.</description><subject>Adjuvants, Immunologic - metabolism</subject><subject>Apoptosis - physiology</subject><subject>Biological and medical sciences</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Digestive system</subject><subject>Fas Ligand Protein</subject><subject>Granzymes</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukocytes - metabolism</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Perforin</subject><subject>Pore Forming Cytotoxic Proteins</subject><subject>RNA, Messenger - metabolism</subject><subject>Serine Endopeptidases - genetics</subject><subject>Serine Endopeptidases - metabolism</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0168-8278</issn><issn>1600-0641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMFO3DAQhi3UCpYtj0DlQ1XBIXQSx7F9RCsKSCv1AIij5TgTMCRxyCRS9-0bdleFy4xG-v5_pI-x0xQuUkiLX3fz0InOlD4DONcg9HwdsEVaACRQ5OkXtviPHLFjohcAEGDyQ3aUghKyALVgj1d_-wGJQux4rLnfjPE1dEjcdRWvnR_jQLyN1dS4MXRP3PWxHyMF4uWGP0-t6ziFbqIYKtfwBicf5w6kb-xr7RrCk_1esoffV_erm2T95_p2dblOvBD5mKD3qZJKlUYIiVlZlqBzZzJZSC1LyKFEKZUAVxuXuUqgMQa9RIWgFAgjluznrrcf4tuENNo2kMemcR3GiawCo6Eo8hmUO9APkWjA2vZDaN2wsSnYd6N2a9S-67IAdmvU6jn3ff9gKlusPqV2Cmfgxx5w5F1TD67zgT64TIFWRvwDUuV_bA</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>JONSSON, J. 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E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-ecc17577b9335e2bbb084a9256585b040be55730af9a2ad3e999ec5e7e0770393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adjuvants, Immunologic - metabolism</topic><topic>Apoptosis - physiology</topic><topic>Biological and medical sciences</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Digestive system</topic><topic>Fas Ligand Protein</topic><topic>Granzymes</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukocytes - metabolism</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Perforin</topic><topic>Pore Forming Cytotoxic Proteins</topic><topic>RNA, Messenger - metabolism</topic><topic>Serine Endopeptidases - genetics</topic><topic>Serine Endopeptidases - metabolism</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JONSSON, J. R</creatorcontrib><creatorcontrib>EDWARDS-SMITH, C. J</creatorcontrib><creatorcontrib>CATANIA, S. C</creatorcontrib><creatorcontrib>MOROTOMI, Y</creatorcontrib><creatorcontrib>HOGAN, P. G</creatorcontrib><creatorcontrib>CLOUSTON, A. D</creatorcontrib><creatorcontrib>BANSAL, A. S</creatorcontrib><creatorcontrib>LYNCH, S. V</creatorcontrib><creatorcontrib>STRONG, R. W</creatorcontrib><creatorcontrib>POWELL, E. E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JONSSON, J. R</au><au>EDWARDS-SMITH, C. J</au><au>CATANIA, S. C</au><au>MOROTOMI, Y</au><au>HOGAN, P. G</au><au>CLOUSTON, A. D</au><au>BANSAL, A. S</au><au>LYNCH, S. V</au><au>STRONG, R. W</au><au>POWELL, E. E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of cytokines and factors modulating apoptosis by human sinusoidal leucocytes</atitle><jtitle>Journal of hepatology</jtitle><addtitle>J Hepatol</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>32</volume><issue>3</issue><spage>392</spage><epage>398</epage><pages>392-398</pages><issn>0168-8278</issn><eissn>1600-0641</eissn><coden>JOHEEC</coden><abstract>Liver sinusoids contain a large population of spontaneously cytotoxic cells (NK cells), CD8+ T cells and macrophages. The physiological role of these leucocytes remains unclear. They may participate in immune surveillance and peripheral tolerance by deleting tumour cells, virus-infected cells and activated T cells as they traffic through the liver. In order to gain further information about the function of these leucocytes within the hepatic sinusoids, we examined their production of immunomodulatory cytokines and apoptosis-related molecules.
Semi-quantitative polymerase chain reaction and immunohistochemistry were used to determine the spontaneous production of cytokines and apoptosis-related molecules by sinusoidal leucocytes isolated from donor liver preservation solution.
In comparison with matched peripheral blood mononuclear cells, sinusoidal leucocytes produced more mRNA for IL-10, IL-15, TNF-alpha, IL-18, IFN-gamma, FasL, perforin and granzyme. IL-4 and IL-12 were not detected and IL-2 was only faintly detected in the liver-derived CD4+ population. Less bcl-2 was expressed in liver-derived CD4+ and CD8+ cells in comparison with matched peripheral blood cell populations.
The cytokines produced spontaneously by sinusoidal leucocytes are consistent with their high level of activation and spontaneous cytotoxicity. Their strong expression of apoptosis-mediating molecules (FasL, perforin, granzyme and TNF-alpha) support a role for these cells in immune surveillance and peripheral tolerance induction.</abstract><cop>Oxford</cop><pub>Elsevier</pub><pmid>10735607</pmid><doi>10.1016/S0168-8278(00)80388-8</doi><tpages>7</tpages></addata></record> |
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| subjects | Adjuvants, Immunologic - metabolism Apoptosis - physiology Biological and medical sciences Cytokines - genetics Cytokines - metabolism Digestive system Fas Ligand Protein Granzymes Humans Investigative techniques, diagnostic techniques (general aspects) Leukocytes - metabolism Liver - cytology Liver - metabolism Medical sciences Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Perforin Pore Forming Cytotoxic Proteins RNA, Messenger - metabolism Serine Endopeptidases - genetics Serine Endopeptidases - metabolism Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism |
| title | Expression of cytokines and factors modulating apoptosis by human sinusoidal leucocytes |
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