Glutathione metabolism and dimorphism in Aureobasidium pullulans

Yeast⟷mycelium morphological transitions of Aureobasidium pullulans are influenced by numerous environmental factors. In general, changes in the glutathione (GSH) metabolism of dimorphic fungi may lead to alterations in the reduced thiol status of the cells that are hypothesised to initialise morpho...

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Veröffentlicht in:Journal of basic microbiology 2001-05, Vol.41 (2), p.131-137
Hauptverfasser: Jürgensen, Claudia Würtz, Jacobsen, Nicklas Raun, Emri, Tamás, Havn Eriksen, Susanne, Pócsi, István
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container_issue 2
container_start_page 131
container_title Journal of basic microbiology
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creator Jürgensen, Claudia Würtz
Jacobsen, Nicklas Raun
Emri, Tamás
Havn Eriksen, Susanne
Pócsi, István
description Yeast⟷mycelium morphological transitions of Aureobasidium pullulans are influenced by numerous environmental factors. In general, changes in the glutathione (GSH) metabolism of dimorphic fungi may lead to alterations in the reduced thiol status of the cells that are hypothesised to initialise morphological transitions. In accordance with this hypothesis, the specific GSH levels found in A. pullulans yeast cells were always significantly higher than those in mycelia. On the other hand, there was no significant difference between the GSH/GSSG redox status of the cells with either yeast or mycelial morphology. The cascade of events leading to morphological transitions was therefore unlikely to proceed via redox modulation of protein thiols. Although there were morphology‐dependent differences in the specific activities of some GSH metabolic enzymes, e.g. glutathione reductase (GR),γ‐glutamyltranspeptidase (γGT), glucose‐6‐phosphate dehydrogenase (G6PD), they were not satisfactory to explain the observed alterations in the intracellular GSH levels. It is noteworthy that very similar specific γGT and G6PD activities were found in cells separated from mixed morphology cultures independently of the actual cell morphology. On the other hand, the specific γGT and G6PD activities of A. pullulans cells sharing the same morphology but separated from pure and mixed morphology cultures showed marked differences.
doi_str_mv 10.1002/1521-4028(200105)41:2<131::AID-JOBM131>3.0.CO;2-#
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In general, changes in the glutathione (GSH) metabolism of dimorphic fungi may lead to alterations in the reduced thiol status of the cells that are hypothesised to initialise morphological transitions. In accordance with this hypothesis, the specific GSH levels found in A. pullulans yeast cells were always significantly higher than those in mycelia. On the other hand, there was no significant difference between the GSH/GSSG redox status of the cells with either yeast or mycelial morphology. The cascade of events leading to morphological transitions was therefore unlikely to proceed via redox modulation of protein thiols. Although there were morphology‐dependent differences in the specific activities of some GSH metabolic enzymes, e.g. glutathione reductase (GR),γ‐glutamyltranspeptidase (γGT), glucose‐6‐phosphate dehydrogenase (G6PD), they were not satisfactory to explain the observed alterations in the intracellular GSH levels. It is noteworthy that very similar specific γGT and G6PD activities were found in cells separated from mixed morphology cultures independently of the actual cell morphology. 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subjects Ascomycota - cytology
Ascomycota - enzymology
Ascomycota - growth & development
Ascomycota - physiology
Aureobasidium pullulans
Culture Media
g-glutamyltranspeptidase
G6PD
G6PD, glucose‐6‐phosphate dehydrogenase
gGT
gGT, g‐glutamyltranspeptidase
glucose-6-phosphate dehydrogenase
glutathione
Glutathione - metabolism
glutathione disulphide
glutathione peroxidase
glutathione reductase
glutathione S-transferase
GPx
GPx, glutathione peroxidase
GR, glutathione reductase
GSH
GSH, glutathione
GSSG
GSSG, glutathione disulphide
GST
GST, glutathione S‐transferase
SD, standard deviation
SOD
SOD, superoxide dismutase
standard deviation
superoxide dismutase
title Glutathione metabolism and dimorphism in Aureobasidium pullulans
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