Ligninolytic enzyme production in selected sub-tropical white rot fungi under different culture conditions
Lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase activities in selected sub‐tropical white rot fungal species from Zimbabwe were determined. The enzyme activities were assayed at varying concentrations of C, N and Mn2+. Manganese peroxidase and laccase activities were the only express...
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description | Lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase activities in selected sub‐tropical white rot fungal species from Zimbabwe were determined. The enzyme activities were assayed at varying concentrations of C, N and Mn2+. Manganese peroxidase and laccase activities were the only expressed activities in the fungi under the culture conditions tested. Trametes species, T. cingulata, T. elegans and T. pocas produced the highest manganese peroxidase activities in a medium containing high carbon and low nitrogen conditions. High nitrogen conditions favoured high manganese peroxidase activity in DSPM95, L. velutinus and Irpex spp. High manganese peroxidase activity was notable for T. versicolor when both carbon and nitrogen in the medium were present at high levels. Laccase production by the isolates was highest under conditions of high nitrogen and those conditions with both nitrogen and carbon at high concentration. Mn2+ concentrations between 11–25 ppm gave the highest manganese peroxidase activity compared to a concentration of 40 ppm or when there was no Mn2+ added. Laccase activity was less influenced by Mn2+ levels. While some laccase activity was produced in the absence of Mn2+, the enzyme levels were higher when Mn2+ was added to the culture medium. |
doi_str_mv | 10.1002/1521-4028(200105)41:2<115::AID-JOBM115>3.0.CO;2-S |
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The enzyme activities were assayed at varying concentrations of C, N and Mn2+. Manganese peroxidase and laccase activities were the only expressed activities in the fungi under the culture conditions tested. Trametes species, T. cingulata, T. elegans and T. pocas produced the highest manganese peroxidase activities in a medium containing high carbon and low nitrogen conditions. High nitrogen conditions favoured high manganese peroxidase activity in DSPM95, L. velutinus and Irpex spp. High manganese peroxidase activity was notable for T. versicolor when both carbon and nitrogen in the medium were present at high levels. Laccase production by the isolates was highest under conditions of high nitrogen and those conditions with both nitrogen and carbon at high concentration. Mn2+ concentrations between 11–25 ppm gave the highest manganese peroxidase activity compared to a concentration of 40 ppm or when there was no Mn2+ added. Laccase activity was less influenced by Mn2+ levels. 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Basic Microbiol</addtitle><description>Lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase activities in selected sub‐tropical white rot fungal species from Zimbabwe were determined. The enzyme activities were assayed at varying concentrations of C, N and Mn2+. Manganese peroxidase and laccase activities were the only expressed activities in the fungi under the culture conditions tested. Trametes species, T. cingulata, T. elegans and T. pocas produced the highest manganese peroxidase activities in a medium containing high carbon and low nitrogen conditions. High nitrogen conditions favoured high manganese peroxidase activity in DSPM95, L. velutinus and Irpex spp. High manganese peroxidase activity was notable for T. versicolor when both carbon and nitrogen in the medium were present at high levels. Laccase production by the isolates was highest under conditions of high nitrogen and those conditions with both nitrogen and carbon at high concentration. Mn2+ concentrations between 11–25 ppm gave the highest manganese peroxidase activity compared to a concentration of 40 ppm or when there was no Mn2+ added. Laccase activity was less influenced by Mn2+ levels. While some laccase activity was produced in the absence of Mn2+, the enzyme levels were higher when Mn2+ was added to the culture medium.</description><subject>Basidiomycota - classification</subject><subject>Basidiomycota - enzymology</subject><subject>Basidiomycota - growth & development</subject><subject>Carbon - metabolism</subject><subject>Culture Media</subject><subject>Laccase</subject><subject>Manganese - metabolism</subject><subject>Nitrogen - metabolism</subject><subject>Oxidoreductases - metabolism</subject><subject>Peroxidases - metabolism</subject><subject>Trametes</subject><issn>0233-111X</issn><issn>1521-4028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd2LEzEUxYMobq3-C5In0YepufmYjyrCWt0PrRZZRfElTDN3drNOMzXJsNa_3pTW-iKITyE3J7_DPYeQCtgEGONPQXHIJOPlY84YMPVEwpQ_B1DT6fH5q-zN4uW7dHkhJmwyWzzj2cUtMjr8uU1GjAuRAcCXI3IvhGvGWFXx6i45ApASpKpG5HpuL511fbeJ1lB0PzcrpGvfN4OJtnfUOhqwQxOxoWFYZtH3a2vqjt5c2YjU95G2g7u0dHANetrYtkWPLlIzdHHwSE3vGrtFhfvkTlt3AR_szzH5dPL64-wsmy9Oz2fH88woxlXGC2FUzkQpqhJyWXDeGoMSBW9FXWPD8haWQgpVLJdV2qhsKyOkKpQouMQSxJg82nHTGt8HDFGvbDDYdbXDfgi6YFVRiuTwLyFnHJhM2jH5sBMa34fgsdVrb1e132hgetuU3qaut6nrXVNagk5TUFqnpvS-KS0007NFerlIzId782G5wuYPcV-NOMRzYzvc_Ifj3w1_jxI323FtiPjjwK39N50XolD68_tTnZ98FTB_q_SZ-AWWpLwF</recordid><startdate>200105</startdate><enddate>200105</enddate><creator>Tekere, Memory</creator><creator>Zvauya, Remigio</creator><creator>Read, John S.</creator><general>WILEY-VCH Verlag Berlin GmbH</general><general>WILEY‐VCH Verlag Berlin GmbH</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200105</creationdate><title>Ligninolytic enzyme production in selected sub-tropical white rot fungi under different culture conditions</title><author>Tekere, Memory ; Zvauya, Remigio ; Read, John S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5025-273c56038398164722fcce4e32f3aaed06f1b34357bb90098f9c345753724e813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Basidiomycota - classification</topic><topic>Basidiomycota - enzymology</topic><topic>Basidiomycota - growth & development</topic><topic>Carbon - metabolism</topic><topic>Culture Media</topic><topic>Laccase</topic><topic>Manganese - metabolism</topic><topic>Nitrogen - metabolism</topic><topic>Oxidoreductases - metabolism</topic><topic>Peroxidases - metabolism</topic><topic>Trametes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tekere, Memory</creatorcontrib><creatorcontrib>Zvauya, Remigio</creatorcontrib><creatorcontrib>Read, John S.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of basic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tekere, Memory</au><au>Zvauya, Remigio</au><au>Read, John S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ligninolytic enzyme production in selected sub-tropical white rot fungi under different culture conditions</atitle><jtitle>Journal of basic microbiology</jtitle><addtitle>J. Basic Microbiol</addtitle><date>2001-05</date><risdate>2001</risdate><volume>41</volume><issue>2</issue><spage>115</spage><epage>129</epage><pages>115-129</pages><issn>0233-111X</issn><eissn>1521-4028</eissn><abstract>Lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase activities in selected sub‐tropical white rot fungal species from Zimbabwe were determined. The enzyme activities were assayed at varying concentrations of C, N and Mn2+. Manganese peroxidase and laccase activities were the only expressed activities in the fungi under the culture conditions tested. Trametes species, T. cingulata, T. elegans and T. pocas produced the highest manganese peroxidase activities in a medium containing high carbon and low nitrogen conditions. High nitrogen conditions favoured high manganese peroxidase activity in DSPM95, L. velutinus and Irpex spp. High manganese peroxidase activity was notable for T. versicolor when both carbon and nitrogen in the medium were present at high levels. Laccase production by the isolates was highest under conditions of high nitrogen and those conditions with both nitrogen and carbon at high concentration. Mn2+ concentrations between 11–25 ppm gave the highest manganese peroxidase activity compared to a concentration of 40 ppm or when there was no Mn2+ added. Laccase activity was less influenced by Mn2+ levels. While some laccase activity was produced in the absence of Mn2+, the enzyme levels were higher when Mn2+ was added to the culture medium.</abstract><cop>Berlin</cop><pub>WILEY-VCH Verlag Berlin GmbH</pub><pmid>11441459</pmid><doi>10.1002/1521-4028(200105)41:2<115::AID-JOBM115>3.0.CO;2-S</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Basidiomycota - classification Basidiomycota - enzymology Basidiomycota - growth & development Carbon - metabolism Culture Media Laccase Manganese - metabolism Nitrogen - metabolism Oxidoreductases - metabolism Peroxidases - metabolism Trametes |
title | Ligninolytic enzyme production in selected sub-tropical white rot fungi under different culture conditions |
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