Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia
This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of nitric oxide (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This i...
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| Veröffentlicht in: | International journal of immunopharmacology 2000-06, Vol.22 (6), p.453-461 |
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| container_title | International journal of immunopharmacology |
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| creator | Kim, Hyung-Min Rim, Hong-Kun Shin, Taekyun Kim, Jeong Joong Park, Seung Taeck Oh, Jae Min Choi, Min Kyu Chung, Yeun Tai Rhee, Hee Sub Jeung, Jae Yeal Lee, Ki Nam Kim, Nam Song Kim, Cheorl-Ho |
| description | This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of nitric oxide (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on
l-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells. |
| doi_str_mv | 10.1016/S0192-0561(00)00011-4 |
| format | Article |
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l-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.</description><identifier>ISSN: 0192-0561</identifier><identifier>EISSN: 1879-3495</identifier><identifier>DOI: 10.1016/S0192-0561(00)00011-4</identifier><identifier>PMID: 10727756</identifier><identifier>CODEN: IJIMDS</identifier><language>eng</language><publisher>Oxford: Elsevier Science</publisher><subject>Animals ; Astrocytes - drug effects ; Astrocytes - metabolism ; Biological and medical sciences ; Blotting, Western ; Cell physiology ; Cell Separation ; Cells, Cultured ; chorionic gonadotropin ; Chorionic Gonadotropin - pharmacology ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; g-Interferon ; Hormonal regulation ; Humans ; Immunohistochemistry ; Indicators and Reagents ; Interferon-gamma - pharmacology ; L-arginine ; Mice ; Mice, Inbred BALB C ; Microglia - drug effects ; Microglia - metabolism ; Molecular and cellular biology ; Nitrates - analysis ; Nitrates - metabolism ; nitric oxide ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - antagonists & inhibitors ; Nitric Oxide Synthase Type I ; Nitrites - analysis ; Nitrites - metabolism ; Nitroarginine - pharmacology ; Recombinant Proteins ; Stimulation, Chemical ; tumor necrosis factor-^a ; Tumor Necrosis Factor-alpha - biosynthesis</subject><ispartof>International journal of immunopharmacology, 2000-06, Vol.22 (6), p.453-461</ispartof><rights>2000</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-5f706b99e814e29fa83a2f55cc54aa7549594d57b18d4bee3b7197435af0dace3</citedby><cites>FETCH-LOGICAL-c421t-5f706b99e814e29fa83a2f55cc54aa7549594d57b18d4bee3b7197435af0dace3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1357919$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10727756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hyung-Min</creatorcontrib><creatorcontrib>Rim, Hong-Kun</creatorcontrib><creatorcontrib>Shin, Taekyun</creatorcontrib><creatorcontrib>Kim, Jeong Joong</creatorcontrib><creatorcontrib>Park, Seung Taeck</creatorcontrib><creatorcontrib>Oh, Jae Min</creatorcontrib><creatorcontrib>Choi, Min Kyu</creatorcontrib><creatorcontrib>Chung, Yeun Tai</creatorcontrib><creatorcontrib>Rhee, Hee Sub</creatorcontrib><creatorcontrib>Jeung, Jae Yeal</creatorcontrib><creatorcontrib>Lee, Ki Nam</creatorcontrib><creatorcontrib>Kim, Nam Song</creatorcontrib><creatorcontrib>Kim, Cheorl-Ho</creatorcontrib><title>Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia</title><title>International journal of immunopharmacology</title><addtitle>Int J Immunopharmacol</addtitle><description>This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of nitric oxide (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on
l-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.</description><subject>Animals</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell physiology</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>chorionic gonadotropin</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>g-Interferon</subject><subject>Hormonal regulation</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Indicators and Reagents</subject><subject>Interferon-gamma - pharmacology</subject><subject>L-arginine</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microglia - drug effects</subject><subject>Microglia - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Nitrates - analysis</subject><subject>Nitrates - metabolism</subject><subject>nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Nitric Oxide Synthase Type I</subject><subject>Nitrites - analysis</subject><subject>Nitrites - metabolism</subject><subject>Nitroarginine - pharmacology</subject><subject>Recombinant Proteins</subject><subject>Stimulation, Chemical</subject><subject>tumor necrosis factor-^a</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><issn>0192-0561</issn><issn>1879-3495</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhq2Kii6Fn0CVA6roIeBJ7Dg-IVTRD6lSD8DZcuxJOyixt3aCuv--2e6qcOtpDvPMzDsPYx-BfwEOzdefHHRVctnAZ87POOcApThgK2iVLmuh5Ru2ekGO2Luc_yyQhKZ6y46Aq0op2azY7dU82lC4-5goBnLFXQzWxynFNYWCgp8d5iLQlJZefCSPRd6E6R4z5aLbFOOcKGAxkkvxbiD7nh32dsj4YV-P2e-LH7_Or8qb28vr8-83pRMVTKXsFW86rbEFgZXubVvbqpfSOSmsVXLJr4WXqoPWiw6x7hRoJWppe-6tw_qYne72rlN8mDFPZqTscBhswDhno7hWClr9KghKVo2qmwWUO3D5JOeEvVknGm3aGOBmq9w8Kzdbn4Zz86zciGXuZH9g7kb0_03tHC_Apz1gs7NDn2xwlP9xtVQatkG_7TBctP0lTCY7wuDQU0I3GR_plSRPYp6dxQ</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Kim, Hyung-Min</creator><creator>Rim, Hong-Kun</creator><creator>Shin, Taekyun</creator><creator>Kim, Jeong Joong</creator><creator>Park, Seung Taeck</creator><creator>Oh, Jae Min</creator><creator>Choi, Min Kyu</creator><creator>Chung, Yeun Tai</creator><creator>Rhee, Hee Sub</creator><creator>Jeung, Jae Yeal</creator><creator>Lee, Ki Nam</creator><creator>Kim, Nam Song</creator><creator>Kim, Cheorl-Ho</creator><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000601</creationdate><title>Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia</title><author>Kim, Hyung-Min ; Rim, Hong-Kun ; Shin, Taekyun ; Kim, Jeong Joong ; Park, Seung Taeck ; Oh, Jae Min ; Choi, Min Kyu ; Chung, Yeun Tai ; Rhee, Hee Sub ; Jeung, Jae Yeal ; Lee, Ki Nam ; Kim, Nam Song ; Kim, Cheorl-Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-5f706b99e814e29fa83a2f55cc54aa7549594d57b18d4bee3b7197435af0dace3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell physiology</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>chorionic gonadotropin</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>g-Interferon</topic><topic>Hormonal regulation</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Indicators and Reagents</topic><topic>Interferon-gamma - pharmacology</topic><topic>L-arginine</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microglia - drug effects</topic><topic>Microglia - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Nitrates - analysis</topic><topic>Nitrates - metabolism</topic><topic>nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Nitric Oxide Synthase Type I</topic><topic>Nitrites - analysis</topic><topic>Nitrites - metabolism</topic><topic>Nitroarginine - pharmacology</topic><topic>Recombinant Proteins</topic><topic>Stimulation, Chemical</topic><topic>tumor necrosis factor-^a</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hyung-Min</creatorcontrib><creatorcontrib>Rim, Hong-Kun</creatorcontrib><creatorcontrib>Shin, Taekyun</creatorcontrib><creatorcontrib>Kim, Jeong Joong</creatorcontrib><creatorcontrib>Park, Seung Taeck</creatorcontrib><creatorcontrib>Oh, Jae Min</creatorcontrib><creatorcontrib>Choi, Min Kyu</creatorcontrib><creatorcontrib>Chung, Yeun Tai</creatorcontrib><creatorcontrib>Rhee, Hee Sub</creatorcontrib><creatorcontrib>Jeung, Jae Yeal</creatorcontrib><creatorcontrib>Lee, Ki Nam</creatorcontrib><creatorcontrib>Kim, Nam Song</creatorcontrib><creatorcontrib>Kim, Cheorl-Ho</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hyung-Min</au><au>Rim, Hong-Kun</au><au>Shin, Taekyun</au><au>Kim, Jeong Joong</au><au>Park, Seung Taeck</au><au>Oh, Jae Min</au><au>Choi, Min Kyu</au><au>Chung, Yeun Tai</au><au>Rhee, Hee Sub</au><au>Jeung, Jae Yeal</au><au>Lee, Ki Nam</au><au>Kim, Nam Song</au><au>Kim, Cheorl-Ho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia</atitle><jtitle>International journal of immunopharmacology</jtitle><addtitle>Int J Immunopharmacol</addtitle><date>2000-06-01</date><risdate>2000</risdate><volume>22</volume><issue>6</issue><spage>453</spage><epage>461</epage><pages>453-461</pages><issn>0192-0561</issn><eissn>1879-3495</eissn><coden>IJIMDS</coden><abstract>This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of nitric oxide (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on
l-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.</abstract><cop>Oxford</cop><pub>Elsevier Science</pub><pmid>10727756</pmid><doi>10.1016/S0192-0561(00)00011-4</doi><tpages>9</tpages></addata></record> |
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| ispartof | International journal of immunopharmacology, 2000-06, Vol.22 (6), p.453-461 |
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| subjects | Animals Astrocytes - drug effects Astrocytes - metabolism Biological and medical sciences Blotting, Western Cell physiology Cell Separation Cells, Cultured chorionic gonadotropin Chorionic Gonadotropin - pharmacology Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology g-Interferon Hormonal regulation Humans Immunohistochemistry Indicators and Reagents Interferon-gamma - pharmacology L-arginine Mice Mice, Inbred BALB C Microglia - drug effects Microglia - metabolism Molecular and cellular biology Nitrates - analysis Nitrates - metabolism nitric oxide Nitric Oxide - biosynthesis Nitric Oxide Synthase - antagonists & inhibitors Nitric Oxide Synthase Type I Nitrites - analysis Nitrites - metabolism Nitroarginine - pharmacology Recombinant Proteins Stimulation, Chemical tumor necrosis factor-^a Tumor Necrosis Factor-alpha - biosynthesis |
| title | Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia |
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