A comparison of different lysis buffers to assess allele dropout from single cells for preimplantation genetic diagnosis

Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) requires high efficiency and accuracy. Allele dropout (ADO), the random amplification failure of one of the two parental alleles, remains the most significant problem in PCR‐based PGD testing since it can result...

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Veröffentlicht in:Prenatal diagnosis 2001-06, Vol.21 (6), p.490-497
Hauptverfasser: Thornhill, Alan R., McGrath, John A., Eady, Robin A. J., Braude, Peter R., Handyside, Alan H.
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container_end_page 497
container_issue 6
container_start_page 490
container_title Prenatal diagnosis
container_volume 21
creator Thornhill, Alan R.
McGrath, John A.
Eady, Robin A. J.
Braude, Peter R.
Handyside, Alan H.
description Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) requires high efficiency and accuracy. Allele dropout (ADO), the random amplification failure of one of the two parental alleles, remains the most significant problem in PCR‐based PGD testing since it can result in serious misdiagnosis for compound heterozygous or autosomal dominant conditions. A number of different strategies (including the use of lysis buffers to break down the cell and make the DNA accessible) have been employed to combat ADO with varying degrees of success, yet there is still no consensus among PGD centres over which lysis buffer should be used (ESHRE PGD Consortium, 1999). To address this issue, PCR amplification of three genes (CFTR, LAMA3 and PKP1) at different chromosomal loci was investigated. Single lymphocytes from individuals heterozygous for mutations within each of the three genes were collected and lysed in either alkaline lysis buffer (ALB) or proteinase K/SDS lysis buffer (PK). PCR amplification efficiencies were comparable between alkaline lysis and proteinase K lysis for PCR products spanning each of the three mutated loci (ΔF508 in CFTR 90% vs 88%; R650X in LAMA3 82% vs 78%; and Y71X in PKP1 91% vs 87%). While there was no appreciable difference between ADO rates between the two lysis buffers for the LAMA3 PCR product (25% vs 26%), there were significant differences in ADO rates between ALB and PK for the CFTR PCR product (0% vs 23%) and the PKP1 PCR product (8% vs 56%). Based on these results, we are currently using ALB in preference to PK/SDS buffer for the lysis of cells in clinical PGD. Copyright © 2001 John Wiley & Sons, Ltd.
doi_str_mv 10.1002/pd.109
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To address this issue, PCR amplification of three genes (CFTR, LAMA3 and PKP1) at different chromosomal loci was investigated. Single lymphocytes from individuals heterozygous for mutations within each of the three genes were collected and lysed in either alkaline lysis buffer (ALB) or proteinase K/SDS lysis buffer (PK). PCR amplification efficiencies were comparable between alkaline lysis and proteinase K lysis for PCR products spanning each of the three mutated loci (ΔF508 in CFTR 90% vs 88%; R650X in LAMA3 82% vs 78%; and Y71X in PKP1 91% vs 87%). While there was no appreciable difference between ADO rates between the two lysis buffers for the LAMA3 PCR product (25% vs 26%), there were significant differences in ADO rates between ALB and PK for the CFTR PCR product (0% vs 23%) and the PKP1 PCR product (8% vs 56%). Based on these results, we are currently using ALB in preference to PK/SDS buffer for the lysis of cells in clinical PGD. 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J.</creatorcontrib><creatorcontrib>Braude, Peter R.</creatorcontrib><creatorcontrib>Handyside, Alan H.</creatorcontrib><title>A comparison of different lysis buffers to assess allele dropout from single cells for preimplantation genetic diagnosis</title><title>Prenatal diagnosis</title><addtitle>Prenat. Diagn</addtitle><description>Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) requires high efficiency and accuracy. Allele dropout (ADO), the random amplification failure of one of the two parental alleles, remains the most significant problem in PCR‐based PGD testing since it can result in serious misdiagnosis for compound heterozygous or autosomal dominant conditions. 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Obstetrics</subject><subject>Humans</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mutation - genetics</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pregnancy</subject><subject>Preimplantation Diagnosis - methods</subject><subject>preimplantation genetic diagnosis (PGD)</subject><subject>single cell</subject><subject>Sterility. 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Assisted procreation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thornhill, Alan R.</creatorcontrib><creatorcontrib>McGrath, John A.</creatorcontrib><creatorcontrib>Eady, Robin A. J.</creatorcontrib><creatorcontrib>Braude, Peter R.</creatorcontrib><creatorcontrib>Handyside, Alan H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Prenatal diagnosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thornhill, Alan R.</au><au>McGrath, John A.</au><au>Eady, Robin A. 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subjects allele dropout (ADO)
Alleles
Biological and medical sciences
Birth control
Buffers
DNA Primers
Female
Gynecology. Andrology. Obstetrics
Humans
Lymphocytes
Male
Medical sciences
Mutation - genetics
PCR
Polymerase Chain Reaction - methods
Pregnancy
Preimplantation Diagnosis - methods
preimplantation genetic diagnosis (PGD)
single cell
Sterility. Assisted procreation
title A comparison of different lysis buffers to assess allele dropout from single cells for preimplantation genetic diagnosis
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