Analysis of missed cleavage sites, tryptophan oxidation and N-terminal pyroglutamylation after in-gel tryptic digestion

Peptide mass fingerprinting is a powerful tool for the identification of proteins. Trypsin is the most widely used enzyme for this purpose. Therefore, 104 protein digests from human Jurkat T cells and Mycobacterium were analyzed considering missed cleavage sites, tryptophan oxidation and N‐terminal...

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Veröffentlicht in:Rapid communications in mass spectrometry 2000-03, Vol.14 (6), p.496-502
Hauptverfasser: Thiede, Bernd, Lamer, Stephanie, Mattow, Jens, Siejak, Frank, Dimmler, Christiane, Rudel, Thomas, Jungblut, Peter R.
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Sprache:eng
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