Migration and Multipotentiality of PSA-NCAM+ Neural Precursors Transplanted in the Developing Brain
By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and forme...
Gespeichert in:
| Veröffentlicht in: | Molecular and cellular neuroscience 2001-06, Vol.17 (6), p.983-1000 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1000 |
|---|---|
| container_issue | 6 |
| container_start_page | 983 |
| container_title | Molecular and cellular neuroscience |
| container_volume | 17 |
| creator | Vitry, Sandrine Avellana-Adalid, Virginia Lachapelle, François Baron-Van Evercooren, Anne |
| description | By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and formed spheres, which were initially enriched in PSA-NCAM+ cells but became enriched in GD3+ oligodendrocyte progenitors after 1 week in B104 contionned medium. One month after their periventricular transplantation into the brain of wild-type and/or shiverer newborn mice, cells from PSA-NCAM+ spheres exhibited a higher rostral migration potential than cells from GD3+ spheres, and clearly contributed to myelination in the olfactory bulb. In shiverer hosts, both sphere populations generated oligodendrocytes with similar myelination potential. In addition PSA-NCAM+ sphere cells generated GFAP+ astrocytes and NeuN+ neurons, depending on their site of insertion. These results evidence the high plasticity of newborn PSA-NCAM+ neural precursors and suggest that they are promising tools for cell therapy of CNS diseases, including myelin disorders. |
| doi_str_mv | 10.1006/mcne.2001.0987 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70972967</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S104474310190987X</els_id><sourcerecordid>18167644</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-67b00cb49f84e0d72b84ea6d463dbc0a851a6cdb7251104ce2cd8829e8b04683</originalsourceid><addsrcrecordid>eNqFkE1P3DAQhq2qVfnqtcfKp16qbG3HsZ3jsgWKxAISe7cce5YaZe1gO0j8exLtSpwqTu8cnnk18yD0nZIFJUT83tkAC0YIXZBWyU_omJK2qdqayc_zzHkleU2P0EnOT4SQhrX1V3REKadcKnWM7No_JlN8DNgEh9djX_wQC4TiTe_LK45bfP-wrG5Xy_UvfAtjMj2-T2DHlGPKeJNMyENvQgGHfcDlH-A_8AJ9HHx4xOfJ-HCGvmxNn-HbIU_R5vJis_pb3dxdXa-WN5WtG1YqITtCbMfbreJAnGTdlEY4LmrXWWJUQ42wrpOsodNnFph1SrEWVEe4UPUp-rmvHVJ8HiEXvfPZQj8dB3HMWpJWslbID0GqqJCC8wlc7EGbYs4JtnpIfmfSq6ZEz_r1rF_P-vWsf1r4cWgeux24d_zgewLUHoDJw4uHpLP1ECw4Pzkt2kX_v-43w3-UBw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18167644</pqid></control><display><type>article</type><title>Migration and Multipotentiality of PSA-NCAM+ Neural Precursors Transplanted in the Developing Brain</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Vitry, Sandrine ; Avellana-Adalid, Virginia ; Lachapelle, François ; Baron-Van Evercooren, Anne</creator><creatorcontrib>Vitry, Sandrine ; Avellana-Adalid, Virginia ; Lachapelle, François ; Baron-Van Evercooren, Anne</creatorcontrib><description>By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and formed spheres, which were initially enriched in PSA-NCAM+ cells but became enriched in GD3+ oligodendrocyte progenitors after 1 week in B104 contionned medium. One month after their periventricular transplantation into the brain of wild-type and/or shiverer newborn mice, cells from PSA-NCAM+ spheres exhibited a higher rostral migration potential than cells from GD3+ spheres, and clearly contributed to myelination in the olfactory bulb. In shiverer hosts, both sphere populations generated oligodendrocytes with similar myelination potential. In addition PSA-NCAM+ sphere cells generated GFAP+ astrocytes and NeuN+ neurons, depending on their site of insertion. These results evidence the high plasticity of newborn PSA-NCAM+ neural precursors and suggest that they are promising tools for cell therapy of CNS diseases, including myelin disorders.</description><identifier>ISSN: 1044-7431</identifier><identifier>EISSN: 1095-9327</identifier><identifier>DOI: 10.1006/mcne.2001.0987</identifier><identifier>PMID: 11414788</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antigens, Differentiation - metabolism ; Astrocytes - cytology ; Astrocytes - metabolism ; Brain - cytology ; Brain - growth & development ; Brain - metabolism ; Brain Tissue Transplantation - methods ; Cell Aggregation - genetics ; Cell Culture Techniques - methods ; Cell Differentiation - physiology ; Cell Lineage - physiology ; Cell Movement - physiology ; Cells, Cultured - cytology ; Cells, Cultured - drug effects ; Cells, Cultured - metabolism ; Central Nervous System Diseases - surgery ; Clone Cells - cytology ; Clone Cells - drug effects ; Clone Cells - metabolism ; Culture Media - pharmacology ; Gangliosides - metabolism ; Gene Expression Regulation, Developmental - physiology ; Glial Fibrillary Acidic Protein - metabolism ; Graft Survival - physiology ; Intermediate Filament Proteins - metabolism ; Mice ; Mice, Transgenic ; Nerve Tissue Proteins ; Nestin ; Neural Cell Adhesion Molecule L1 ; Neural Cell Adhesion Molecules - genetics ; Neural Cell Adhesion Molecules - metabolism ; Neurons - cytology ; Neurons - metabolism ; Neurons - transplantation ; Oligodendroglia - cytology ; Oligodendroglia - metabolism ; polysialylation ; Sialic Acids - genetics ; Sialic Acids - metabolism ; Stem Cell Transplantation ; Stem Cells - cytology ; Stem Cells - metabolism ; Thalamus - cytology ; Thalamus - growth & development ; Thalamus - surgery</subject><ispartof>Molecular and cellular neuroscience, 2001-06, Vol.17 (6), p.983-1000</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-67b00cb49f84e0d72b84ea6d463dbc0a851a6cdb7251104ce2cd8829e8b04683</citedby><cites>FETCH-LOGICAL-c352t-67b00cb49f84e0d72b84ea6d463dbc0a851a6cdb7251104ce2cd8829e8b04683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mcne.2001.0987$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11414788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vitry, Sandrine</creatorcontrib><creatorcontrib>Avellana-Adalid, Virginia</creatorcontrib><creatorcontrib>Lachapelle, François</creatorcontrib><creatorcontrib>Baron-Van Evercooren, Anne</creatorcontrib><title>Migration and Multipotentiality of PSA-NCAM+ Neural Precursors Transplanted in the Developing Brain</title><title>Molecular and cellular neuroscience</title><addtitle>Mol Cell Neurosci</addtitle><description>By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and formed spheres, which were initially enriched in PSA-NCAM+ cells but became enriched in GD3+ oligodendrocyte progenitors after 1 week in B104 contionned medium. One month after their periventricular transplantation into the brain of wild-type and/or shiverer newborn mice, cells from PSA-NCAM+ spheres exhibited a higher rostral migration potential than cells from GD3+ spheres, and clearly contributed to myelination in the olfactory bulb. In shiverer hosts, both sphere populations generated oligodendrocytes with similar myelination potential. In addition PSA-NCAM+ sphere cells generated GFAP+ astrocytes and NeuN+ neurons, depending on their site of insertion. These results evidence the high plasticity of newborn PSA-NCAM+ neural precursors and suggest that they are promising tools for cell therapy of CNS diseases, including myelin disorders.</description><subject>Animals</subject><subject>Antigens, Differentiation - metabolism</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - metabolism</subject><subject>Brain - cytology</subject><subject>Brain - growth & development</subject><subject>Brain - metabolism</subject><subject>Brain Tissue Transplantation - methods</subject><subject>Cell Aggregation - genetics</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage - physiology</subject><subject>Cell Movement - physiology</subject><subject>Cells, Cultured - cytology</subject><subject>Cells, Cultured - drug effects</subject><subject>Cells, Cultured - metabolism</subject><subject>Central Nervous System Diseases - surgery</subject><subject>Clone Cells - cytology</subject><subject>Clone Cells - drug effects</subject><subject>Clone Cells - metabolism</subject><subject>Culture Media - pharmacology</subject><subject>Gangliosides - metabolism</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Graft Survival - physiology</subject><subject>Intermediate Filament Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Nerve Tissue Proteins</subject><subject>Nestin</subject><subject>Neural Cell Adhesion Molecule L1</subject><subject>Neural Cell Adhesion Molecules - genetics</subject><subject>Neural Cell Adhesion Molecules - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Neurons - transplantation</subject><subject>Oligodendroglia - cytology</subject><subject>Oligodendroglia - metabolism</subject><subject>polysialylation</subject><subject>Sialic Acids - genetics</subject><subject>Sialic Acids - metabolism</subject><subject>Stem Cell Transplantation</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Thalamus - cytology</subject><subject>Thalamus - growth & development</subject><subject>Thalamus - surgery</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq2qVfnqtcfKp16qbG3HsZ3jsgWKxAISe7cce5YaZe1gO0j8exLtSpwqTu8cnnk18yD0nZIFJUT83tkAC0YIXZBWyU_omJK2qdqayc_zzHkleU2P0EnOT4SQhrX1V3REKadcKnWM7No_JlN8DNgEh9djX_wQC4TiTe_LK45bfP-wrG5Xy_UvfAtjMj2-T2DHlGPKeJNMyENvQgGHfcDlH-A_8AJ9HHx4xOfJ-HCGvmxNn-HbIU_R5vJis_pb3dxdXa-WN5WtG1YqITtCbMfbreJAnGTdlEY4LmrXWWJUQ42wrpOsodNnFph1SrEWVEe4UPUp-rmvHVJ8HiEXvfPZQj8dB3HMWpJWslbID0GqqJCC8wlc7EGbYs4JtnpIfmfSq6ZEz_r1rF_P-vWsf1r4cWgeux24d_zgewLUHoDJw4uHpLP1ECw4Pzkt2kX_v-43w3-UBw</recordid><startdate>200106</startdate><enddate>200106</enddate><creator>Vitry, Sandrine</creator><creator>Avellana-Adalid, Virginia</creator><creator>Lachapelle, François</creator><creator>Baron-Van Evercooren, Anne</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200106</creationdate><title>Migration and Multipotentiality of PSA-NCAM+ Neural Precursors Transplanted in the Developing Brain</title><author>Vitry, Sandrine ; Avellana-Adalid, Virginia ; Lachapelle, François ; Baron-Van Evercooren, Anne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-67b00cb49f84e0d72b84ea6d463dbc0a851a6cdb7251104ce2cd8829e8b04683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antigens, Differentiation - metabolism</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - metabolism</topic><topic>Brain - cytology</topic><topic>Brain - growth & development</topic><topic>Brain - metabolism</topic><topic>Brain Tissue Transplantation - methods</topic><topic>Cell Aggregation - genetics</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Lineage - physiology</topic><topic>Cell Movement - physiology</topic><topic>Cells, Cultured - cytology</topic><topic>Cells, Cultured - drug effects</topic><topic>Cells, Cultured - metabolism</topic><topic>Central Nervous System Diseases - surgery</topic><topic>Clone Cells - cytology</topic><topic>Clone Cells - drug effects</topic><topic>Clone Cells - metabolism</topic><topic>Culture Media - pharmacology</topic><topic>Gangliosides - metabolism</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Graft Survival - physiology</topic><topic>Intermediate Filament Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Nerve Tissue Proteins</topic><topic>Nestin</topic><topic>Neural Cell Adhesion Molecule L1</topic><topic>Neural Cell Adhesion Molecules - genetics</topic><topic>Neural Cell Adhesion Molecules - metabolism</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Neurons - transplantation</topic><topic>Oligodendroglia - cytology</topic><topic>Oligodendroglia - metabolism</topic><topic>polysialylation</topic><topic>Sialic Acids - genetics</topic><topic>Sialic Acids - metabolism</topic><topic>Stem Cell Transplantation</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Thalamus - cytology</topic><topic>Thalamus - growth & development</topic><topic>Thalamus - surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vitry, Sandrine</creatorcontrib><creatorcontrib>Avellana-Adalid, Virginia</creatorcontrib><creatorcontrib>Lachapelle, François</creatorcontrib><creatorcontrib>Baron-Van Evercooren, Anne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vitry, Sandrine</au><au>Avellana-Adalid, Virginia</au><au>Lachapelle, François</au><au>Baron-Van Evercooren, Anne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Migration and Multipotentiality of PSA-NCAM+ Neural Precursors Transplanted in the Developing Brain</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2001-06</date><risdate>2001</risdate><volume>17</volume><issue>6</issue><spage>983</spage><epage>1000</epage><pages>983-1000</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and formed spheres, which were initially enriched in PSA-NCAM+ cells but became enriched in GD3+ oligodendrocyte progenitors after 1 week in B104 contionned medium. One month after their periventricular transplantation into the brain of wild-type and/or shiverer newborn mice, cells from PSA-NCAM+ spheres exhibited a higher rostral migration potential than cells from GD3+ spheres, and clearly contributed to myelination in the olfactory bulb. In shiverer hosts, both sphere populations generated oligodendrocytes with similar myelination potential. In addition PSA-NCAM+ sphere cells generated GFAP+ astrocytes and NeuN+ neurons, depending on their site of insertion. These results evidence the high plasticity of newborn PSA-NCAM+ neural precursors and suggest that they are promising tools for cell therapy of CNS diseases, including myelin disorders.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11414788</pmid><doi>10.1006/mcne.2001.0987</doi><tpages>18</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1044-7431 |
| ispartof | Molecular and cellular neuroscience, 2001-06, Vol.17 (6), p.983-1000 |
| issn | 1044-7431 1095-9327 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70972967 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antigens, Differentiation - metabolism Astrocytes - cytology Astrocytes - metabolism Brain - cytology Brain - growth & development Brain - metabolism Brain Tissue Transplantation - methods Cell Aggregation - genetics Cell Culture Techniques - methods Cell Differentiation - physiology Cell Lineage - physiology Cell Movement - physiology Cells, Cultured - cytology Cells, Cultured - drug effects Cells, Cultured - metabolism Central Nervous System Diseases - surgery Clone Cells - cytology Clone Cells - drug effects Clone Cells - metabolism Culture Media - pharmacology Gangliosides - metabolism Gene Expression Regulation, Developmental - physiology Glial Fibrillary Acidic Protein - metabolism Graft Survival - physiology Intermediate Filament Proteins - metabolism Mice Mice, Transgenic Nerve Tissue Proteins Nestin Neural Cell Adhesion Molecule L1 Neural Cell Adhesion Molecules - genetics Neural Cell Adhesion Molecules - metabolism Neurons - cytology Neurons - metabolism Neurons - transplantation Oligodendroglia - cytology Oligodendroglia - metabolism polysialylation Sialic Acids - genetics Sialic Acids - metabolism Stem Cell Transplantation Stem Cells - cytology Stem Cells - metabolism Thalamus - cytology Thalamus - growth & development Thalamus - surgery |
| title | Migration and Multipotentiality of PSA-NCAM+ Neural Precursors Transplanted in the Developing Brain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T20%3A37%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Migration%20and%20Multipotentiality%20of%20PSA-NCAM+%20Neural%20Precursors%20Transplanted%20in%20the%20Developing%20Brain&rft.jtitle=Molecular%20and%20cellular%20neuroscience&rft.au=Vitry,%20Sandrine&rft.date=2001-06&rft.volume=17&rft.issue=6&rft.spage=983&rft.epage=1000&rft.pages=983-1000&rft.issn=1044-7431&rft.eissn=1095-9327&rft_id=info:doi/10.1006/mcne.2001.0987&rft_dat=%3Cproquest_cross%3E18167644%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18167644&rft_id=info:pmid/11414788&rft_els_id=S104474310190987X&rfr_iscdi=true |