PEAZ-1: A new human prostate neoplastic epithelial cell line
BACKGROUND The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ‐1 (prostate epithelial Arizona‐1). METHODS The PEAZ‐1 cells were...
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| Veröffentlicht in: | The Prostate 2001-07, Vol.48 (2), p.79-92 |
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| container_title | The Prostate |
| container_volume | 48 |
| creator | Schmelz, Monika Cress, Anne E. Barrera, Jean McDaniel, Kathy M. Davis, Tracy L. Fuchs, Laura Dalkin, Bruce L. Nagle, Raymond B. |
| description | BACKGROUND
The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ‐1 (prostate epithelial Arizona‐1).
METHODS
The PEAZ‐1 cells were grown from a primary human prostate carcinoma specimen obtained from radical prostatectomy. The isolated cells were characterized by immunobiochemistry, immunohistochemistry, and tumorigenicity studies.
RESULTS
PEAZ‐1 cells are near diploid, tumorigenic, and androgen independent for cell growth. PEAZ‐1 cells express N‐cadherin, α‐ and β‐catenins, and p120 at cell–cell contacts, cytoplasmic laminin 5, vinculin, paxillin, and phosphotyrosine at focal adhesions, vimentin, and cytokeratins 8 and 18. They do not express plakoglobin, E‐cadherin, and PSA, and do not form desmosomes and hemidesomomes. PEAZ‐1 respond to ocadaic acid, a pro‐apoptotic agent, by expression of p53.
CONCLUSIONS
PEAZ‐1 cells is a human prostate cancer cell line that has a number of mesenchymal characteristics. Prostate 48:79–92, 2001. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/pros.1084 |
| format | Article |
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The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ‐1 (prostate epithelial Arizona‐1).
METHODS
The PEAZ‐1 cells were grown from a primary human prostate carcinoma specimen obtained from radical prostatectomy. The isolated cells were characterized by immunobiochemistry, immunohistochemistry, and tumorigenicity studies.
RESULTS
PEAZ‐1 cells are near diploid, tumorigenic, and androgen independent for cell growth. PEAZ‐1 cells express N‐cadherin, α‐ and β‐catenins, and p120 at cell–cell contacts, cytoplasmic laminin 5, vinculin, paxillin, and phosphotyrosine at focal adhesions, vimentin, and cytokeratins 8 and 18. They do not express plakoglobin, E‐cadherin, and PSA, and do not form desmosomes and hemidesomomes. PEAZ‐1 respond to ocadaic acid, a pro‐apoptotic agent, by expression of p53.
CONCLUSIONS
PEAZ‐1 cells is a human prostate cancer cell line that has a number of mesenchymal characteristics. Prostate 48:79–92, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.1084</identifier><identifier>PMID: 11433418</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>androgens ; Androgens - pharmacology ; cadherins ; Cadherins - pharmacology ; catenins ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry ; in vitro cell culture ; integrins ; Integrins - analysis ; laminin 5 ; Male ; prostate ; Prostatectomy ; Prostatic Neoplasms - pathology ; Specimen Handling ; Tumor Cells, Cultured</subject><ispartof>The Prostate, 2001-07, Vol.48 (2), p.79-92</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4254-a88cc8df375022c2192ece48778b0c338b01a8e79613aa1a1b4b1e7382f2f8d63</citedby><cites>FETCH-LOGICAL-c4254-a88cc8df375022c2192ece48778b0c338b01a8e79613aa1a1b4b1e7382f2f8d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.1084$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.1084$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11433418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmelz, Monika</creatorcontrib><creatorcontrib>Cress, Anne E.</creatorcontrib><creatorcontrib>Barrera, Jean</creatorcontrib><creatorcontrib>McDaniel, Kathy M.</creatorcontrib><creatorcontrib>Davis, Tracy L.</creatorcontrib><creatorcontrib>Fuchs, Laura</creatorcontrib><creatorcontrib>Dalkin, Bruce L.</creatorcontrib><creatorcontrib>Nagle, Raymond B.</creatorcontrib><title>PEAZ-1: A new human prostate neoplastic epithelial cell line</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ‐1 (prostate epithelial Arizona‐1).
METHODS
The PEAZ‐1 cells were grown from a primary human prostate carcinoma specimen obtained from radical prostatectomy. The isolated cells were characterized by immunobiochemistry, immunohistochemistry, and tumorigenicity studies.
RESULTS
PEAZ‐1 cells are near diploid, tumorigenic, and androgen independent for cell growth. PEAZ‐1 cells express N‐cadherin, α‐ and β‐catenins, and p120 at cell–cell contacts, cytoplasmic laminin 5, vinculin, paxillin, and phosphotyrosine at focal adhesions, vimentin, and cytokeratins 8 and 18. They do not express plakoglobin, E‐cadherin, and PSA, and do not form desmosomes and hemidesomomes. PEAZ‐1 respond to ocadaic acid, a pro‐apoptotic agent, by expression of p53.
CONCLUSIONS
PEAZ‐1 cells is a human prostate cancer cell line that has a number of mesenchymal characteristics. Prostate 48:79–92, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>androgens</subject><subject>Androgens - pharmacology</subject><subject>cadherins</subject><subject>Cadherins - pharmacology</subject><subject>catenins</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>in vitro cell culture</subject><subject>integrins</subject><subject>Integrins - analysis</subject><subject>laminin 5</subject><subject>Male</subject><subject>prostate</subject><subject>Prostatectomy</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Specimen Handling</subject><subject>Tumor Cells, Cultured</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF9PwjAUxRujEUQf_AJmTyY-TPpvtBhfkABqiBDEkPjSdKUL1Q7mugX59nbZok--3N40v3tyzgHgEsFbBCHuZvnO-Y3TI9BGsM9CCGl0DNoQMxhSRFgLnDn3AaGnIT4FLYQoIRTxNrifjwbvIboLBsFW74NNmcptUOkVstD-a5dZ6QqjAp2ZYqOtkTZQ2trAmq0-ByeJtE5fNG8HvI1Hy-FjOJ1NnoaDaagojmgoOVeKrxPCIoixwqiPtdKUM8ZjqAjxE0muWb-HiJRIopjGSDPCcYITvu6RDriudb2xr1K7QqTGVS6kN1g6wXxmjCLmwZsaVD6By3UistykMj8IBEVVlaiiiaoqz141omWc6vUf2XTjgW4N7I3Vh_-VxHwxe20kw_rCuEJ__17I_FP0mE8vVi8TsXgeLx_4iog5-QEOO4DF</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Schmelz, Monika</creator><creator>Cress, Anne E.</creator><creator>Barrera, Jean</creator><creator>McDaniel, Kathy M.</creator><creator>Davis, Tracy L.</creator><creator>Fuchs, Laura</creator><creator>Dalkin, Bruce L.</creator><creator>Nagle, Raymond B.</creator><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>PEAZ-1: A new human prostate neoplastic epithelial cell line</title><author>Schmelz, Monika ; Cress, Anne E. ; Barrera, Jean ; McDaniel, Kathy M. ; Davis, Tracy L. ; Fuchs, Laura ; Dalkin, Bruce L. ; Nagle, Raymond B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4254-a88cc8df375022c2192ece48778b0c338b01a8e79613aa1a1b4b1e7382f2f8d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>androgens</topic><topic>Androgens - pharmacology</topic><topic>cadherins</topic><topic>Cadherins - pharmacology</topic><topic>catenins</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>in vitro cell culture</topic><topic>integrins</topic><topic>Integrins - analysis</topic><topic>laminin 5</topic><topic>Male</topic><topic>prostate</topic><topic>Prostatectomy</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Specimen Handling</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmelz, Monika</creatorcontrib><creatorcontrib>Cress, Anne E.</creatorcontrib><creatorcontrib>Barrera, Jean</creatorcontrib><creatorcontrib>McDaniel, Kathy M.</creatorcontrib><creatorcontrib>Davis, Tracy L.</creatorcontrib><creatorcontrib>Fuchs, Laura</creatorcontrib><creatorcontrib>Dalkin, Bruce L.</creatorcontrib><creatorcontrib>Nagle, Raymond B.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmelz, Monika</au><au>Cress, Anne E.</au><au>Barrera, Jean</au><au>McDaniel, Kathy M.</au><au>Davis, Tracy L.</au><au>Fuchs, Laura</au><au>Dalkin, Bruce L.</au><au>Nagle, Raymond B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PEAZ-1: A new human prostate neoplastic epithelial cell line</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>48</volume><issue>2</issue><spage>79</spage><epage>92</epage><pages>79-92</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><abstract>BACKGROUND
The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ‐1 (prostate epithelial Arizona‐1).
METHODS
The PEAZ‐1 cells were grown from a primary human prostate carcinoma specimen obtained from radical prostatectomy. The isolated cells were characterized by immunobiochemistry, immunohistochemistry, and tumorigenicity studies.
RESULTS
PEAZ‐1 cells are near diploid, tumorigenic, and androgen independent for cell growth. PEAZ‐1 cells express N‐cadherin, α‐ and β‐catenins, and p120 at cell–cell contacts, cytoplasmic laminin 5, vinculin, paxillin, and phosphotyrosine at focal adhesions, vimentin, and cytokeratins 8 and 18. They do not express plakoglobin, E‐cadherin, and PSA, and do not form desmosomes and hemidesomomes. PEAZ‐1 respond to ocadaic acid, a pro‐apoptotic agent, by expression of p53.
CONCLUSIONS
PEAZ‐1 cells is a human prostate cancer cell line that has a number of mesenchymal characteristics. Prostate 48:79–92, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11433418</pmid><doi>10.1002/pros.1084</doi><tpages>14</tpages></addata></record> |
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| ispartof | The Prostate, 2001-07, Vol.48 (2), p.79-92 |
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| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | androgens Androgens - pharmacology cadherins Cadherins - pharmacology catenins Gene Expression Regulation, Neoplastic Humans Immunohistochemistry in vitro cell culture integrins Integrins - analysis laminin 5 Male prostate Prostatectomy Prostatic Neoplasms - pathology Specimen Handling Tumor Cells, Cultured |
| title | PEAZ-1: A new human prostate neoplastic epithelial cell line |
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