Identification of genes regulated by UV/salicylic acid
Purpose : Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB....
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| Veröffentlicht in: | Int. J. Radiat. Biol 2000-02, Vol.76 (2), p.189-198 |
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| creator | Paunesku, C.-M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T. |
| description | Purpose : Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments described here, differential-display RT-PCR was used to identify those cDNAs that are differentially modulated by UV radiation with and without the addition of salicylic acid. Materials and methods : Differential-display RT-PCR was used to identify differentially expressed genes. Results : Eight such cDNAs are presented: lactate dehydrogenase (LDH-beta), nuclear encoded mitochondrial NADH ubiquinone reductase 24kDa (NDUFV2), elongation initiation factor 4B (eIF4B), nuclear dots protein SP100, nuclear encoded mitochondrial ATPase inhibitor (IF1), a cDNA similar to a subunit of yeast CCAAT transcription factor HAP5, and two expressed sequence tags (AA187906 and AA513156). Conclusions : Sequences of four of these genes contained NF-kappaB DNA binding sites of the type that may attract transrepressor p55/p55 NF-kappaB homodimers. Down-regulation of these genes upon UV irradiation may contribute to increased cell survival via suppression of p53 independent apoptosis. |
| doi_str_mv | 10.1080/095530000138844 |
| format | Article |
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Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T.</creator><creatorcontrib>Paunesku, C.-M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T. ; Argonne National Lab. (ANL), Argonne, IL (United States)</creatorcontrib><description>Purpose : Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments described here, differential-display RT-PCR was used to identify those cDNAs that are differentially modulated by UV radiation with and without the addition of salicylic acid. Materials and methods : Differential-display RT-PCR was used to identify differentially expressed genes. Results : Eight such cDNAs are presented: lactate dehydrogenase (LDH-beta), nuclear encoded mitochondrial NADH ubiquinone reductase 24kDa (NDUFV2), elongation initiation factor 4B (eIF4B), nuclear dots protein SP100, nuclear encoded mitochondrial ATPase inhibitor (IF1), a cDNA similar to a subunit of yeast CCAAT transcription factor HAP5, and two expressed sequence tags (AA187906 and AA513156). Conclusions : Sequences of four of these genes contained NF-kappaB DNA binding sites of the type that may attract transrepressor p55/p55 NF-kappaB homodimers. Down-regulation of these genes upon UV irradiation may contribute to increased cell survival via suppression of p53 independent apoptosis.</description><identifier>ISSN: 0955-3002</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/095530000138844</identifier><identifier>PMID: 10716640</identifier><language>eng</language><publisher>London: Informa UK Ltd</publisher><subject>Adenosine Triphosphatases - genetics ; APOPTOSIS ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; Cell Line ; DNA ; ELONGATION ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - radiation effects ; GENES ; Genes, p53 - physiology ; Humans ; Indomethacin - pharmacology ; IRRADIATION ; L-Lactate Dehydrogenase - genetics ; LACTATE DEHYDROGENASE ; MODULATION ; Molecular and cellular biology ; Molecular genetics ; NF-kappa B - physiology ; OXIDOREDUCTASES ; PROTEINS ; RADIATIONS ; RNA, Messenger - analysis ; SALICYLIC ACID ; Salicylic Acid - pharmacology ; Space life sciences ; TRANSCRIPTION FACTORS ; UBIQUINONE ; Ultraviolet Rays ; YEASTS</subject><ispartof>Int. J. Radiat. Biol, 2000-02, Vol.76 (2), p.189-198</ispartof><rights>2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2000</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-3a596eb9001d627fff820a8f4b7b4ee6ceb1e08cd25bc3fe6e962a182db2350d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/095530000138844$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/095530000138844$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,881,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1279516$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10716640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/938342$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Paunesku, C.-M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T.</creatorcontrib><creatorcontrib>Argonne National Lab. (ANL), Argonne, IL (United States)</creatorcontrib><title>Identification of genes regulated by UV/salicylic acid</title><title>Int. J. Radiat. Biol</title><addtitle>Int J Radiat Biol</addtitle><description>Purpose : Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments described here, differential-display RT-PCR was used to identify those cDNAs that are differentially modulated by UV radiation with and without the addition of salicylic acid. Materials and methods : Differential-display RT-PCR was used to identify differentially expressed genes. Results : Eight such cDNAs are presented: lactate dehydrogenase (LDH-beta), nuclear encoded mitochondrial NADH ubiquinone reductase 24kDa (NDUFV2), elongation initiation factor 4B (eIF4B), nuclear dots protein SP100, nuclear encoded mitochondrial ATPase inhibitor (IF1), a cDNA similar to a subunit of yeast CCAAT transcription factor HAP5, and two expressed sequence tags (AA187906 and AA513156). Conclusions : Sequences of four of these genes contained NF-kappaB DNA binding sites of the type that may attract transrepressor p55/p55 NF-kappaB homodimers. Down-regulation of these genes upon UV irradiation may contribute to increased cell survival via suppression of p53 independent apoptosis.</description><subject>Adenosine Triphosphatases - genetics</subject><subject>APOPTOSIS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>DNA</subject><subject>ELONGATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - radiation effects</subject><subject>GENES</subject><subject>Genes, p53 - physiology</subject><subject>Humans</subject><subject>Indomethacin - pharmacology</subject><subject>IRRADIATION</subject><subject>L-Lactate Dehydrogenase - genetics</subject><subject>LACTATE DEHYDROGENASE</subject><subject>MODULATION</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>NF-kappa B - physiology</subject><subject>OXIDOREDUCTASES</subject><subject>PROTEINS</subject><subject>RADIATIONS</subject><subject>RNA, Messenger - analysis</subject><subject>SALICYLIC ACID</subject><subject>Salicylic Acid - pharmacology</subject><subject>Space life sciences</subject><subject>TRANSCRIPTION FACTORS</subject><subject>UBIQUINONE</subject><subject>Ultraviolet Rays</subject><subject>YEASTS</subject><issn>0955-3002</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kN1LHDEUxYNU6qp99k2mIL6tm4-ZTMa3Iv0QhL5UX0MmuXEj2USTDGX_e7PMltqCgUvCze-c3ByEzgi-IljgFR66juG6CBOibQ_QgjBOl6z2P6DF7raeMT1Cxzk_VYxiJj6iI4J7wnmLF4jfGgjFWadVcTE00TaPECA3CR4nrwqYZtw29w-rrLzT21qN0s6cokOrfIZP-_0E3X_7-uvmx_Lu5_fbmy93S922Q1ky1Q0cxqHOZzjtrbWCYiVsO_ZjC8A1jASw0IZ2o2YWOAycKiKoGSnrsGEn6PPsG3NxMmtXQK91DAF0kQMTrKWVuZyZ5xRfJshFblzW4L0KEKcse1xNO84ruJpBnWLOCax8Tm6j0lYSLHdxyv_irIrzvfU0bsC84ef8KnCxB1TWytukgnb5L0f7oSO7l69nzAUb00b9jskbWdTWx_RHw94fYvhHvAbly1qrBPIpTinU_N_9wCvkEZ9K</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>Paunesku, C.-M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20000201</creationdate><title>Identification of genes regulated by UV/salicylic acid</title><author>Paunesku, C.-M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-3a596eb9001d627fff820a8f4b7b4ee6ceb1e08cd25bc3fe6e962a182db2350d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adenosine Triphosphatases - genetics</topic><topic>APOPTOSIS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>DNA</topic><topic>ELONGATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - radiation effects</topic><topic>GENES</topic><topic>Genes, p53 - physiology</topic><topic>Humans</topic><topic>Indomethacin - pharmacology</topic><topic>IRRADIATION</topic><topic>L-Lactate Dehydrogenase - genetics</topic><topic>LACTATE DEHYDROGENASE</topic><topic>MODULATION</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>NF-kappa B - physiology</topic><topic>OXIDOREDUCTASES</topic><topic>PROTEINS</topic><topic>RADIATIONS</topic><topic>RNA, Messenger - analysis</topic><topic>SALICYLIC ACID</topic><topic>Salicylic Acid - pharmacology</topic><topic>Space life sciences</topic><topic>TRANSCRIPTION FACTORS</topic><topic>UBIQUINONE</topic><topic>Ultraviolet Rays</topic><topic>YEASTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paunesku, C.-M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T.</creatorcontrib><creatorcontrib>Argonne National Lab. (ANL), Argonne, IL (United States)</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Int. J. Radiat. Biol</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paunesku, C.-M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak, T.</au><aucorp>Argonne National Lab. (ANL), Argonne, IL (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of genes regulated by UV/salicylic acid</atitle><jtitle>Int. J. Radiat. Biol</jtitle><addtitle>Int J Radiat Biol</addtitle><date>2000-02-01</date><risdate>2000</risdate><volume>76</volume><issue>2</issue><spage>189</spage><epage>198</epage><pages>189-198</pages><issn>0955-3002</issn><eissn>1362-3095</eissn><abstract>Purpose : Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments described here, differential-display RT-PCR was used to identify those cDNAs that are differentially modulated by UV radiation with and without the addition of salicylic acid. Materials and methods : Differential-display RT-PCR was used to identify differentially expressed genes. Results : Eight such cDNAs are presented: lactate dehydrogenase (LDH-beta), nuclear encoded mitochondrial NADH ubiquinone reductase 24kDa (NDUFV2), elongation initiation factor 4B (eIF4B), nuclear dots protein SP100, nuclear encoded mitochondrial ATPase inhibitor (IF1), a cDNA similar to a subunit of yeast CCAAT transcription factor HAP5, and two expressed sequence tags (AA187906 and AA513156). Conclusions : Sequences of four of these genes contained NF-kappaB DNA binding sites of the type that may attract transrepressor p55/p55 NF-kappaB homodimers. Down-regulation of these genes upon UV irradiation may contribute to increased cell survival via suppression of p53 independent apoptosis.</abstract><cop>London</cop><pub>Informa UK Ltd</pub><pmid>10716640</pmid><doi>10.1080/095530000138844</doi><tpages>10</tpages></addata></record> |
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| subjects | Adenosine Triphosphatases - genetics APOPTOSIS BASIC BIOLOGICAL SCIENCES Biological and medical sciences Cell Line DNA ELONGATION Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation - drug effects Gene Expression Regulation - radiation effects GENES Genes, p53 - physiology Humans Indomethacin - pharmacology IRRADIATION L-Lactate Dehydrogenase - genetics LACTATE DEHYDROGENASE MODULATION Molecular and cellular biology Molecular genetics NF-kappa B - physiology OXIDOREDUCTASES PROTEINS RADIATIONS RNA, Messenger - analysis SALICYLIC ACID Salicylic Acid - pharmacology Space life sciences TRANSCRIPTION FACTORS UBIQUINONE Ultraviolet Rays YEASTS |
| title | Identification of genes regulated by UV/salicylic acid |
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