Occurrence of Shiga toxin-producing Escherichia coli in fecal samples from children with diarrhea and from healthy zebu cattle in Uganda
Fecal samples collected from 237 diarrheic infants in Kampala, Uganda and from 159 healthy cattle from a ranch in the Central Region of Uganda were investigated for the presence of Escherichia coli O157 and other types of Shiga toxin-producing E. coli (STEC). E. coli O157 were not detected in 150 st...
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| Veröffentlicht in: | International journal of food microbiology 2001-05, Vol.66 (1), p.95-101 |
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| creator | Kaddu-Mulindwa, D.H. Aisu, T. Gleier, K. Zimmermann, S. Beutin, L. |
| description | Fecal samples collected from 237 diarrheic infants in Kampala, Uganda and from 159 healthy cattle from a ranch in the Central Region of Uganda were investigated for the presence of
Escherichia coli O157 and other types of Shiga toxin-producing
E. coli (STEC).
E. coli O157 were not detected in 150 stool samples from children which were cultivated on sorbitol MacConkey agar. A search for all types of STEC performed on 87 additional human stool samples with an enzyme-immunoassay for Shiga toxins (Premier EHEC) was also negative. Forty-two stool samples from infants were additionally investigated for enteropathogenic
E. coli (EPEC) by DNA-hybridization with an
eae-specific gene probe. Compared to STEC, EPEC were frequent and found in six (14.3%) of these 42 randomly selected stool specimens. We were further interested in the role of cattle as a reservoir for STEC in Uganda. STEC were isolated from 45 of 159 cattle from a herd in the Central Region of Uganda. STEC strains from cattle belonged to 16 different O- and nine different H-types and nine O:H types were identical to those found in bovine STEC from other continents. Only one bovine STEC strain was positive for the
eae-gene, and O-groups associated with enterohemorrhagic
E. coli (EHEC) types (O26, O103, O111, O145 and O157) were not found. Our report demonstrates that STEC are not frequent in urban children in Uganda, but domestic cattle were identified as an important natural reservoir for these organisms in this country. |
| doi_str_mv | 10.1016/S0168-1605(00)00493-1 |
| format | Article |
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Escherichia coli O157 and other types of Shiga toxin-producing
E. coli (STEC).
E. coli O157 were not detected in 150 stool samples from children which were cultivated on sorbitol MacConkey agar. A search for all types of STEC performed on 87 additional human stool samples with an enzyme-immunoassay for Shiga toxins (Premier EHEC) was also negative. Forty-two stool samples from infants were additionally investigated for enteropathogenic
E. coli (EPEC) by DNA-hybridization with an
eae-specific gene probe. Compared to STEC, EPEC were frequent and found in six (14.3%) of these 42 randomly selected stool specimens. We were further interested in the role of cattle as a reservoir for STEC in Uganda. STEC were isolated from 45 of 159 cattle from a herd in the Central Region of Uganda. STEC strains from cattle belonged to 16 different O- and nine different H-types and nine O:H types were identical to those found in bovine STEC from other continents. Only one bovine STEC strain was positive for the
eae-gene, and O-groups associated with enterohemorrhagic
E. coli (EHEC) types (O26, O103, O111, O145 and O157) were not found. Our report demonstrates that STEC are not frequent in urban children in Uganda, but domestic cattle were identified as an important natural reservoir for these organisms in this country.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/S0168-1605(00)00493-1</identifier><identifier>PMID: 11407553</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Africa ; Animals ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Biological and medical sciences ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - microbiology ; Diarrhea ; Diarrhea, Infantile - epidemiology ; Diarrhea, Infantile - microbiology ; Disease Reservoirs - veterinary ; DNA, Bacterial - analysis ; Escherichia coli ; Escherichia coli Infections - epidemiology ; Escherichia coli O157 - growth & development ; Escherichia coli O157 - isolation & purification ; Escherichia coli O157 - pathogenicity ; Feces - microbiology ; Human bacterial diseases ; Humans ; Immunoenzyme Techniques ; Infant ; Infant, Newborn ; Infants ; Infectious diseases ; Medical sciences ; Shiga toxin ; Shiga toxin-producing Escherichia coli ; Shiga Toxins - biosynthesis ; Uganda ; Uganda - epidemiology ; Urban Population ; Zoonoses</subject><ispartof>International journal of food microbiology, 2001-05, Vol.66 (1), p.95-101</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-42f5af168d365396d5c5d842d30d151e206afab4177e759b850404ee76769abd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-1605(00)00493-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3548,23929,23930,25139,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14404515$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11407553$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaddu-Mulindwa, D.H.</creatorcontrib><creatorcontrib>Aisu, T.</creatorcontrib><creatorcontrib>Gleier, K.</creatorcontrib><creatorcontrib>Zimmermann, S.</creatorcontrib><creatorcontrib>Beutin, L.</creatorcontrib><title>Occurrence of Shiga toxin-producing Escherichia coli in fecal samples from children with diarrhea and from healthy zebu cattle in Uganda</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Fecal samples collected from 237 diarrheic infants in Kampala, Uganda and from 159 healthy cattle from a ranch in the Central Region of Uganda were investigated for the presence of
Escherichia coli O157 and other types of Shiga toxin-producing
E. coli (STEC).
E. coli O157 were not detected in 150 stool samples from children which were cultivated on sorbitol MacConkey agar. A search for all types of STEC performed on 87 additional human stool samples with an enzyme-immunoassay for Shiga toxins (Premier EHEC) was also negative. Forty-two stool samples from infants were additionally investigated for enteropathogenic
E. coli (EPEC) by DNA-hybridization with an
eae-specific gene probe. Compared to STEC, EPEC were frequent and found in six (14.3%) of these 42 randomly selected stool specimens. We were further interested in the role of cattle as a reservoir for STEC in Uganda. STEC were isolated from 45 of 159 cattle from a herd in the Central Region of Uganda. STEC strains from cattle belonged to 16 different O- and nine different H-types and nine O:H types were identical to those found in bovine STEC from other continents. Only one bovine STEC strain was positive for the
eae-gene, and O-groups associated with enterohemorrhagic
E. coli (EHEC) types (O26, O103, O111, O145 and O157) were not found. Our report demonstrates that STEC are not frequent in urban children in Uganda, but domestic cattle were identified as an important natural reservoir for these organisms in this country.</description><subject>Africa</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - microbiology</subject><subject>Diarrhea</subject><subject>Diarrhea, Infantile - epidemiology</subject><subject>Diarrhea, Infantile - microbiology</subject><subject>Disease Reservoirs - veterinary</subject><subject>DNA, Bacterial - analysis</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli O157 - growth & development</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Escherichia coli O157 - pathogenicity</subject><subject>Feces - microbiology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infants</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Shiga toxin</subject><subject>Shiga toxin-producing Escherichia coli</subject><subject>Shiga Toxins - biosynthesis</subject><subject>Uganda</subject><subject>Uganda - epidemiology</subject><subject>Urban Population</subject><subject>Zoonoses</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi1ERZfCI4B8AcEhZby24-RUoapApUo9lJ4txx5vjJxksROgPAGPjbe7ao-92LLmm3_8z0_IGwanDFj96aYcTcVqkB8APgKIllfsGVmxRrUVFzU8J6sH5Ji8zPkHAEjO4QU5ZkyAkpKvyL9ra5eUcLRIJ09v-rAxdJ7-hLHapsktNowbepFtjynYPhhqpxhoGKlHayLNZthGzNSnaaClHl2Ror_D3FMXTEo9GmpGt6-XR5z7O_oXu4VaM88Rd0q3m0KYV-TIm5jx9eE-IbdfLr6ff6uurr9enn--qqxQYq7E2kvjiy_Ha8nb2kkrXSPWjoNjkuEaauNNJ5hSqGTbNRIECERVq7o1neMn5P1et9j7uWCe9RCyxRjNiNOStYK2ZqKBJ0HWgGqBtwWUe9CmKeeEXm9TGEy60wz0Lit9n5XeBaEB9H1WmpW-t4cBSzege-w6hFOAdwfA5LJsn8xoQ37kRHEmmSzc2Z7DsrdfAZPONuwSdSGhnbWbwhNf-Q_MOrEi</recordid><startdate>20010521</startdate><enddate>20010521</enddate><creator>Kaddu-Mulindwa, D.H.</creator><creator>Aisu, T.</creator><creator>Gleier, K.</creator><creator>Zimmermann, S.</creator><creator>Beutin, L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20010521</creationdate><title>Occurrence of Shiga toxin-producing Escherichia coli in fecal samples from children with diarrhea and from healthy zebu cattle in Uganda</title><author>Kaddu-Mulindwa, D.H. ; Aisu, T. ; Gleier, K. ; Zimmermann, S. ; Beutin, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-42f5af168d365396d5c5d842d30d151e206afab4177e759b850404ee76769abd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Africa</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - microbiology</topic><topic>Diarrhea</topic><topic>Diarrhea, Infantile - epidemiology</topic><topic>Diarrhea, Infantile - microbiology</topic><topic>Disease Reservoirs - veterinary</topic><topic>DNA, Bacterial - analysis</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli O157 - growth & development</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Escherichia coli O157 - pathogenicity</topic><topic>Feces - microbiology</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infants</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Shiga toxin</topic><topic>Shiga toxin-producing Escherichia coli</topic><topic>Shiga Toxins - biosynthesis</topic><topic>Uganda</topic><topic>Uganda - epidemiology</topic><topic>Urban Population</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaddu-Mulindwa, D.H.</creatorcontrib><creatorcontrib>Aisu, T.</creatorcontrib><creatorcontrib>Gleier, K.</creatorcontrib><creatorcontrib>Zimmermann, S.</creatorcontrib><creatorcontrib>Beutin, L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaddu-Mulindwa, D.H.</au><au>Aisu, T.</au><au>Gleier, K.</au><au>Zimmermann, S.</au><au>Beutin, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence of Shiga toxin-producing Escherichia coli in fecal samples from children with diarrhea and from healthy zebu cattle in Uganda</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2001-05-21</date><risdate>2001</risdate><volume>66</volume><issue>1</issue><spage>95</spage><epage>101</epage><pages>95-101</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Fecal samples collected from 237 diarrheic infants in Kampala, Uganda and from 159 healthy cattle from a ranch in the Central Region of Uganda were investigated for the presence of
Escherichia coli O157 and other types of Shiga toxin-producing
E. coli (STEC).
E. coli O157 were not detected in 150 stool samples from children which were cultivated on sorbitol MacConkey agar. A search for all types of STEC performed on 87 additional human stool samples with an enzyme-immunoassay for Shiga toxins (Premier EHEC) was also negative. Forty-two stool samples from infants were additionally investigated for enteropathogenic
E. coli (EPEC) by DNA-hybridization with an
eae-specific gene probe. Compared to STEC, EPEC were frequent and found in six (14.3%) of these 42 randomly selected stool specimens. We were further interested in the role of cattle as a reservoir for STEC in Uganda. STEC were isolated from 45 of 159 cattle from a herd in the Central Region of Uganda. STEC strains from cattle belonged to 16 different O- and nine different H-types and nine O:H types were identical to those found in bovine STEC from other continents. Only one bovine STEC strain was positive for the
eae-gene, and O-groups associated with enterohemorrhagic
E. coli (EHEC) types (O26, O103, O111, O145 and O157) were not found. Our report demonstrates that STEC are not frequent in urban children in Uganda, but domestic cattle were identified as an important natural reservoir for these organisms in this country.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11407553</pmid><doi>10.1016/S0168-1605(00)00493-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2001-05, Vol.66 (1), p.95-101 |
| issn | 0168-1605 1879-3460 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Africa Animals Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Cattle Cattle Diseases - epidemiology Cattle Diseases - microbiology Diarrhea Diarrhea, Infantile - epidemiology Diarrhea, Infantile - microbiology Disease Reservoirs - veterinary DNA, Bacterial - analysis Escherichia coli Escherichia coli Infections - epidemiology Escherichia coli O157 - growth & development Escherichia coli O157 - isolation & purification Escherichia coli O157 - pathogenicity Feces - microbiology Human bacterial diseases Humans Immunoenzyme Techniques Infant Infant, Newborn Infants Infectious diseases Medical sciences Shiga toxin Shiga toxin-producing Escherichia coli Shiga Toxins - biosynthesis Uganda Uganda - epidemiology Urban Population Zoonoses |
| title | Occurrence of Shiga toxin-producing Escherichia coli in fecal samples from children with diarrhea and from healthy zebu cattle in Uganda |
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