Vasodilatory actions of the dietary peptide carnosine
The objective of this study was to test the hypothesis that the dietary dipeptide carnosine (β-alanine- l-histidine) causes direct decreases in arterial tone. Isolated descending thoracic aortic rings from male Sprague-Dawley rats were used for all studies. Preconstriction of vessels was accomplishe...
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| Veröffentlicht in: | Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2000-03, Vol.16 (3), p.168-172 |
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| creator | Ririe, Douglas G Roberts, Pamela R Shouse, Miyuki N Zaloga, Gary P |
| description | The objective of this study was to test the hypothesis that the dietary dipeptide carnosine (β-alanine-
l-histidine) causes direct decreases in arterial tone. Isolated descending thoracic aortic rings from male Sprague-Dawley rats were used for all studies. Preconstriction of vessels was accomplished with phenylephrine. Carnosine (0.625–20 mM) produced dose-dependent vascular relaxation (
P < 0.05) that was independent of endothelium. The constituent amino acid
l-histidine did not produce any significant relaxation over the same dose range, whereas β-alanine actually produced dose-dependent vasoconstriction (
P < 0.05). The soluble guanylate cyclase inhibitor methylene blue (10
−5 M) significantly decreased the relaxation produced by carnosine (
P < 0.05). Measurement of cyclic GMP in the presence and absence of methylene blue after carnosine and phenylephrine exposure was also done. Methylene blue 10
−5 M resulted in a decrease in cyclic GMP levels from 65.3 ± 15.6 fmol/mg protein to 8.6 ± 0.9 fmol/mg of protein (
P = 0.001). We conclude that carnosine produces relaxation of isolated rat aorta independent of endothelium. The effect of carnosine is at least in part mediated via cyclic GMP production and is not reproduced by its constituent amino acids,
l-histidine and β-alanine. |
| doi_str_mv | 10.1016/S0899-9007(99)00268-3 |
| format | Article |
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l-histidine) causes direct decreases in arterial tone. Isolated descending thoracic aortic rings from male Sprague-Dawley rats were used for all studies. Preconstriction of vessels was accomplished with phenylephrine. Carnosine (0.625–20 mM) produced dose-dependent vascular relaxation (
P < 0.05) that was independent of endothelium. The constituent amino acid
l-histidine did not produce any significant relaxation over the same dose range, whereas β-alanine actually produced dose-dependent vasoconstriction (
P < 0.05). The soluble guanylate cyclase inhibitor methylene blue (10
−5 M) significantly decreased the relaxation produced by carnosine (
P < 0.05). Measurement of cyclic GMP in the presence and absence of methylene blue after carnosine and phenylephrine exposure was also done. Methylene blue 10
−5 M resulted in a decrease in cyclic GMP levels from 65.3 ± 15.6 fmol/mg protein to 8.6 ± 0.9 fmol/mg of protein (
P = 0.001). We conclude that carnosine produces relaxation of isolated rat aorta independent of endothelium. The effect of carnosine is at least in part mediated via cyclic GMP production and is not reproduced by its constituent amino acids,
l-histidine and β-alanine.</description><identifier>ISSN: 0899-9007</identifier><identifier>EISSN: 1873-1244</identifier><identifier>DOI: 10.1016/S0899-9007(99)00268-3</identifier><identifier>PMID: 10705070</identifier><identifier>CODEN: NUTRER</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; aorta ; Aorta, Thoracic - drug effects ; Aorta, Thoracic - physiology ; Biological and medical sciences ; Blood vessels and receptors ; carnosine ; Carnosine - pharmacology ; cyclic GMP ; Cyclic GMP - metabolism ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Guanylate Cyclase - antagonists & inhibitors ; l-histidine ; Male ; Methylene Blue - pharmacology ; peptides ; Phenylephrine - pharmacology ; Rats ; Rats, Sprague-Dawley ; vasodilation ; Vasodilation - drug effects ; Vertebrates: cardiovascular system ; β-alanine</subject><ispartof>Nutrition (Burbank, Los Angeles County, Calif.), 2000-03, Vol.16 (3), p.168-172</ispartof><rights>2000 Elsevier Science Inc.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-df061c7f32b90f023b02803b45916647c0ebc2fd51e5176fac0edfe4911e5e323</citedby><cites>FETCH-LOGICAL-c456t-df061c7f32b90f023b02803b45916647c0ebc2fd51e5176fac0edfe4911e5e323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0899-9007(99)00268-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1296154$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10705070$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ririe, Douglas G</creatorcontrib><creatorcontrib>Roberts, Pamela R</creatorcontrib><creatorcontrib>Shouse, Miyuki N</creatorcontrib><creatorcontrib>Zaloga, Gary P</creatorcontrib><title>Vasodilatory actions of the dietary peptide carnosine</title><title>Nutrition (Burbank, Los Angeles County, Calif.)</title><addtitle>Nutrition</addtitle><description>The objective of this study was to test the hypothesis that the dietary dipeptide carnosine (β-alanine-
l-histidine) causes direct decreases in arterial tone. Isolated descending thoracic aortic rings from male Sprague-Dawley rats were used for all studies. Preconstriction of vessels was accomplished with phenylephrine. Carnosine (0.625–20 mM) produced dose-dependent vascular relaxation (
P < 0.05) that was independent of endothelium. The constituent amino acid
l-histidine did not produce any significant relaxation over the same dose range, whereas β-alanine actually produced dose-dependent vasoconstriction (
P < 0.05). The soluble guanylate cyclase inhibitor methylene blue (10
−5 M) significantly decreased the relaxation produced by carnosine (
P < 0.05). Measurement of cyclic GMP in the presence and absence of methylene blue after carnosine and phenylephrine exposure was also done. Methylene blue 10
−5 M resulted in a decrease in cyclic GMP levels from 65.3 ± 15.6 fmol/mg protein to 8.6 ± 0.9 fmol/mg of protein (
P = 0.001). We conclude that carnosine produces relaxation of isolated rat aorta independent of endothelium. The effect of carnosine is at least in part mediated via cyclic GMP production and is not reproduced by its constituent amino acids,
l-histidine and β-alanine.</description><subject>Animals</subject><subject>aorta</subject><subject>Aorta, Thoracic - drug effects</subject><subject>Aorta, Thoracic - physiology</subject><subject>Biological and medical sciences</subject><subject>Blood vessels and receptors</subject><subject>carnosine</subject><subject>Carnosine - pharmacology</subject><subject>cyclic GMP</subject><subject>Cyclic GMP - metabolism</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guanylate Cyclase - antagonists & inhibitors</subject><subject>l-histidine</subject><subject>Male</subject><subject>Methylene Blue - pharmacology</subject><subject>peptides</subject><subject>Phenylephrine - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>vasodilation</subject><subject>Vasodilation - drug effects</subject><subject>Vertebrates: cardiovascular system</subject><subject>β-alanine</subject><issn>0899-9007</issn><issn>1873-1244</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMoznh5BKULEV1UT9I2naxEBm8w4MLLNqTJCUY6TU06gm9v5oK6cxEO_Hzn5Ocj5IjCBQXKL59gIkQuAOozIc4BGJ_kxRYZ00ld5JSV5TYZ_yAjshfjOwBQwcUuGVGooUpvTKpXFb1xrRp8-MqUHpzvYuZtNrxhZhwOKsU99oMzmGkVOh9dhwdkx6o24uFm7pOX25vn6X0-e7x7mF7Pcl1WfMiNBU51bQvWCLDAigbYBIqmrATlvKw1YKOZNRXFitbcqhQYi6WgKcCCFfvkdH23D_5jgXGQcxc1tq3q0C-irEFwShkksFqDOvgYA1rZBzdP3SUFufQlV77kUoZMc-VLFmnvePPBopmj-bO1FpSAkw2golatDarTLv5yLBWoyoRdrTFMNj4dBhm1w06jcQH1II13_zT5BtByhko</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Ririe, Douglas G</creator><creator>Roberts, Pamela R</creator><creator>Shouse, Miyuki N</creator><creator>Zaloga, Gary P</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Vasodilatory actions of the dietary peptide carnosine</title><author>Ririe, Douglas G ; Roberts, Pamela R ; Shouse, Miyuki N ; Zaloga, Gary P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-df061c7f32b90f023b02803b45916647c0ebc2fd51e5176fac0edfe4911e5e323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>aorta</topic><topic>Aorta, Thoracic - drug effects</topic><topic>Aorta, Thoracic - physiology</topic><topic>Biological and medical sciences</topic><topic>Blood vessels and receptors</topic><topic>carnosine</topic><topic>Carnosine - pharmacology</topic><topic>cyclic GMP</topic><topic>Cyclic GMP - metabolism</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guanylate Cyclase - antagonists & inhibitors</topic><topic>l-histidine</topic><topic>Male</topic><topic>Methylene Blue - pharmacology</topic><topic>peptides</topic><topic>Phenylephrine - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>vasodilation</topic><topic>Vasodilation - drug effects</topic><topic>Vertebrates: cardiovascular system</topic><topic>β-alanine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ririe, Douglas G</creatorcontrib><creatorcontrib>Roberts, Pamela R</creatorcontrib><creatorcontrib>Shouse, Miyuki N</creatorcontrib><creatorcontrib>Zaloga, Gary P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nutrition (Burbank, Los Angeles County, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ririe, Douglas G</au><au>Roberts, Pamela R</au><au>Shouse, Miyuki N</au><au>Zaloga, Gary P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vasodilatory actions of the dietary peptide carnosine</atitle><jtitle>Nutrition (Burbank, Los Angeles County, Calif.)</jtitle><addtitle>Nutrition</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>16</volume><issue>3</issue><spage>168</spage><epage>172</epage><pages>168-172</pages><issn>0899-9007</issn><eissn>1873-1244</eissn><coden>NUTRER</coden><abstract>The objective of this study was to test the hypothesis that the dietary dipeptide carnosine (β-alanine-
l-histidine) causes direct decreases in arterial tone. Isolated descending thoracic aortic rings from male Sprague-Dawley rats were used for all studies. Preconstriction of vessels was accomplished with phenylephrine. Carnosine (0.625–20 mM) produced dose-dependent vascular relaxation (
P < 0.05) that was independent of endothelium. The constituent amino acid
l-histidine did not produce any significant relaxation over the same dose range, whereas β-alanine actually produced dose-dependent vasoconstriction (
P < 0.05). The soluble guanylate cyclase inhibitor methylene blue (10
−5 M) significantly decreased the relaxation produced by carnosine (
P < 0.05). Measurement of cyclic GMP in the presence and absence of methylene blue after carnosine and phenylephrine exposure was also done. Methylene blue 10
−5 M resulted in a decrease in cyclic GMP levels from 65.3 ± 15.6 fmol/mg protein to 8.6 ± 0.9 fmol/mg of protein (
P = 0.001). We conclude that carnosine produces relaxation of isolated rat aorta independent of endothelium. The effect of carnosine is at least in part mediated via cyclic GMP production and is not reproduced by its constituent amino acids,
l-histidine and β-alanine.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10705070</pmid><doi>10.1016/S0899-9007(99)00268-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0899-9007 |
| ispartof | Nutrition (Burbank, Los Angeles County, Calif.), 2000-03, Vol.16 (3), p.168-172 |
| issn | 0899-9007 1873-1244 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals aorta Aorta, Thoracic - drug effects Aorta, Thoracic - physiology Biological and medical sciences Blood vessels and receptors carnosine Carnosine - pharmacology cyclic GMP Cyclic GMP - metabolism Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Guanylate Cyclase - antagonists & inhibitors l-histidine Male Methylene Blue - pharmacology peptides Phenylephrine - pharmacology Rats Rats, Sprague-Dawley vasodilation Vasodilation - drug effects Vertebrates: cardiovascular system β-alanine |
| title | Vasodilatory actions of the dietary peptide carnosine |
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