Canine vagus nerve stores cholecystokinin-58 and -8 but releases only cholecystokinin-8 upon electrical vagal stimulation
Cholecystokinin-58 has been shown to be the major form of cholecystokinin (CCK) released to the circulation upon lumenal stimulation of the small intestine in humans and dogs. In anesthetized dogs, electrical vagal stimulation evokes pancreatic exocrine secretion that is in part mediated through the...
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| Veröffentlicht in: | Regulatory peptides 2000-02, Vol.87 (1), p.1-7 |
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| creator | Chang, Ta-min Thagesen, Helle Lee, Kae Yol Roth, Francis L Chey, William Y |
| description | Cholecystokinin-58 has been shown to be the major form of cholecystokinin (CCK) released to the circulation upon lumenal stimulation of the small intestine in humans and dogs. In anesthetized dogs, electrical vagal stimulation evokes pancreatic exocrine secretion that is in part mediated through the release of CCK. We studied the molecular form of CCK stored in canine vagus nerves and that released into circulation upon electrical vagal stimulation. Gel filtration and radioimmunoassay of the water and acid extracts of canine vagus nerves indicated CCK-8 (35%) and CCK-58 (65%) as the major molecular forms in the vagus nerve. Both forms of CCK isolated from the vagal extracts were equally bioactive as the standard CCK-8 and CCK-58, respectively, in stimulation of amylase release from isolated rat pancreatic acini. Analysis of plasma collected after electrical vagal stimulation indicated that CCK-8 is the only form released into the circulation. The release of CCK-8 upon electrical vagal stimulation was not affected by application of lidocaine to the upper small intestinal mucosa, suggesting that it was released from vagal nerve terminals. |
| doi_str_mv | 10.1016/S0167-0115(99)00090-7 |
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In anesthetized dogs, electrical vagal stimulation evokes pancreatic exocrine secretion that is in part mediated through the release of CCK. We studied the molecular form of CCK stored in canine vagus nerves and that released into circulation upon electrical vagal stimulation. Gel filtration and radioimmunoassay of the water and acid extracts of canine vagus nerves indicated CCK-8 (35%) and CCK-58 (65%) as the major molecular forms in the vagus nerve. Both forms of CCK isolated from the vagal extracts were equally bioactive as the standard CCK-8 and CCK-58, respectively, in stimulation of amylase release from isolated rat pancreatic acini. Analysis of plasma collected after electrical vagal stimulation indicated that CCK-8 is the only form released into the circulation. The release of CCK-8 upon electrical vagal stimulation was not affected by application of lidocaine to the upper small intestinal mucosa, suggesting that it was released from vagal nerve terminals.</description><identifier>ISSN: 0167-0115</identifier><identifier>EISSN: 1873-1686</identifier><identifier>DOI: 10.1016/S0167-0115(99)00090-7</identifier><identifier>PMID: 10710281</identifier><identifier>CODEN: REPPDY</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Amylase release ; Animals ; Biological and medical sciences ; CCK bioassay ; Cholecystokinin - metabolism ; Dogs ; Electric Stimulation ; Female ; Fundamental and applied biological sciences. Psychology ; Gastrointestinal hormones ; Male ; Pancreatic acini ; Sincalide - metabolism ; Vagus Nerve - metabolism ; Vertebrates: digestive system</subject><ispartof>Regulatory peptides, 2000-02, Vol.87 (1), p.1-7</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-ca04396cde49fa038edb2666c49c31c81ba2ab8ac935648222d1b23c9ee4324f3</citedby><cites>FETCH-LOGICAL-c390t-ca04396cde49fa038edb2666c49c31c81ba2ab8ac935648222d1b23c9ee4324f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0167011599000907$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1275023$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10710281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Ta-min</creatorcontrib><creatorcontrib>Thagesen, Helle</creatorcontrib><creatorcontrib>Lee, Kae Yol</creatorcontrib><creatorcontrib>Roth, Francis L</creatorcontrib><creatorcontrib>Chey, William Y</creatorcontrib><title>Canine vagus nerve stores cholecystokinin-58 and -8 but releases only cholecystokinin-8 upon electrical vagal stimulation</title><title>Regulatory peptides</title><addtitle>Regul Pept</addtitle><description>Cholecystokinin-58 has been shown to be the major form of cholecystokinin (CCK) released to the circulation upon lumenal stimulation of the small intestine in humans and dogs. In anesthetized dogs, electrical vagal stimulation evokes pancreatic exocrine secretion that is in part mediated through the release of CCK. We studied the molecular form of CCK stored in canine vagus nerves and that released into circulation upon electrical vagal stimulation. Gel filtration and radioimmunoassay of the water and acid extracts of canine vagus nerves indicated CCK-8 (35%) and CCK-58 (65%) as the major molecular forms in the vagus nerve. Both forms of CCK isolated from the vagal extracts were equally bioactive as the standard CCK-8 and CCK-58, respectively, in stimulation of amylase release from isolated rat pancreatic acini. Analysis of plasma collected after electrical vagal stimulation indicated that CCK-8 is the only form released into the circulation. The release of CCK-8 upon electrical vagal stimulation was not affected by application of lidocaine to the upper small intestinal mucosa, suggesting that it was released from vagal nerve terminals.</description><subject>Amylase release</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CCK bioassay</subject><subject>Cholecystokinin - metabolism</subject><subject>Dogs</subject><subject>Electric Stimulation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrointestinal hormones</subject><subject>Male</subject><subject>Pancreatic acini</subject><subject>Sincalide - metabolism</subject><subject>Vagus Nerve - metabolism</subject><subject>Vertebrates: digestive system</subject><issn>0167-0115</issn><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1rHCEYwHEpLc0m7UdI8VBCepj0UWdGPYWw9A0CObQ9i-M8k5rM6kZnFvbb180uTSGHXBThp4_8CTllcMGAtZ9_lkVWwFhzrvUnANBQyVdkwZQUFWtV-5os_pEjcpzzHQBrpBRvyREDyYArtiDbpQ0-IN3Y2znTgGmDNE8xYabuTxzRbcvp3hdTNYra0NNK0W6eaMIRbS4shnH7zCo6r2OgxbgpeWfH3YCy5smv5tFOPoZ35M1gx4zvD_sJ-f31y6_l9-r65tuP5dV15YSGqXIWaqFb12OtBwtCYd_xtm1drZ1gTrHOctsp67Ro2lpxznvWceE0Yi14PYgTcrZ_d53iw4x5MiufHY6jDRjnbCToUgV4gc0euhRzTjiYdfIrm7aGgdk1N4_NzS6o0do8Njey3PtwGDB3K-z_u7WPXMDHA7C5pBiSDc7nJ8dlU8YXdrlnWGpsPCaTncfgsPepZDR99C_85C9xYp9C</recordid><startdate>20000208</startdate><enddate>20000208</enddate><creator>Chang, Ta-min</creator><creator>Thagesen, Helle</creator><creator>Lee, Kae Yol</creator><creator>Roth, Francis L</creator><creator>Chey, William Y</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000208</creationdate><title>Canine vagus nerve stores cholecystokinin-58 and -8 but releases only cholecystokinin-8 upon electrical vagal stimulation</title><author>Chang, Ta-min ; Thagesen, Helle ; Lee, Kae Yol ; Roth, Francis L ; Chey, William Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-ca04396cde49fa038edb2666c49c31c81ba2ab8ac935648222d1b23c9ee4324f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amylase release</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CCK bioassay</topic><topic>Cholecystokinin - metabolism</topic><topic>Dogs</topic><topic>Electric Stimulation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrointestinal hormones</topic><topic>Male</topic><topic>Pancreatic acini</topic><topic>Sincalide - metabolism</topic><topic>Vagus Nerve - metabolism</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Ta-min</creatorcontrib><creatorcontrib>Thagesen, Helle</creatorcontrib><creatorcontrib>Lee, Kae Yol</creatorcontrib><creatorcontrib>Roth, Francis L</creatorcontrib><creatorcontrib>Chey, William Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Regulatory peptides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Ta-min</au><au>Thagesen, Helle</au><au>Lee, Kae Yol</au><au>Roth, Francis L</au><au>Chey, William Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Canine vagus nerve stores cholecystokinin-58 and -8 but releases only cholecystokinin-8 upon electrical vagal stimulation</atitle><jtitle>Regulatory peptides</jtitle><addtitle>Regul Pept</addtitle><date>2000-02-08</date><risdate>2000</risdate><volume>87</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0167-0115</issn><eissn>1873-1686</eissn><coden>REPPDY</coden><abstract>Cholecystokinin-58 has been shown to be the major form of cholecystokinin (CCK) released to the circulation upon lumenal stimulation of the small intestine in humans and dogs. In anesthetized dogs, electrical vagal stimulation evokes pancreatic exocrine secretion that is in part mediated through the release of CCK. We studied the molecular form of CCK stored in canine vagus nerves and that released into circulation upon electrical vagal stimulation. Gel filtration and radioimmunoassay of the water and acid extracts of canine vagus nerves indicated CCK-8 (35%) and CCK-58 (65%) as the major molecular forms in the vagus nerve. Both forms of CCK isolated from the vagal extracts were equally bioactive as the standard CCK-8 and CCK-58, respectively, in stimulation of amylase release from isolated rat pancreatic acini. Analysis of plasma collected after electrical vagal stimulation indicated that CCK-8 is the only form released into the circulation. The release of CCK-8 upon electrical vagal stimulation was not affected by application of lidocaine to the upper small intestinal mucosa, suggesting that it was released from vagal nerve terminals.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10710281</pmid><doi>10.1016/S0167-0115(99)00090-7</doi><tpages>7</tpages></addata></record> |
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| ispartof | Regulatory peptides, 2000-02, Vol.87 (1), p.1-7 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amylase release Animals Biological and medical sciences CCK bioassay Cholecystokinin - metabolism Dogs Electric Stimulation Female Fundamental and applied biological sciences. Psychology Gastrointestinal hormones Male Pancreatic acini Sincalide - metabolism Vagus Nerve - metabolism Vertebrates: digestive system |
| title | Canine vagus nerve stores cholecystokinin-58 and -8 but releases only cholecystokinin-8 upon electrical vagal stimulation |
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