Photochemical destruction of the Bcl-2 oncoprotein during photodynamic therapy with the phthalocyanine photosensitizer Pc 4
Photodynamic therapy (PDT), utilizing a photosensitizer and visible light, causes localized oxidative damage. With the mitochondrial photosensitizer Pc 4, PDT induces apoptosis, yet its molecular targets are not known. Here, the anti-apoptotic protein Bcl-2 is shown to be highly sensitive to PDT, as...
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| description | Photodynamic therapy (PDT), utilizing a photosensitizer and visible light, causes localized oxidative damage. With the mitochondrial photosensitizer Pc 4, PDT induces apoptosis, yet its molecular targets are not known. Here, the anti-apoptotic protein Bcl-2 is shown to be highly sensitive to PDT, as judged on Western blots by the disappearance of anti-Bcl-2-reactive material from the position of the native 26 kDa protein. The loss of Bcl-2 was PDT dose dependent and was observed for both endogenous and overexpressed Bcl-2 in several cell lines, immediately after PDT, and with chilled cells. It was accompanied by a trace of a 23-kDa cleavage product as well as high-molecular weight products that may result from photochemical crosslinking. PDT-induced Bcl-2 loss occurred in MCF-7 cells that do not express caspase-3 or in the presence of protease inhibitors, but was prevented, along with the induction of apoptosis, by the singlet oxygen scavenger L-histidine. Loss of FLAG-Bcl-2 was observed with both anti-FLAG and anti-Bcl-2 antibodies, indicating loss of native protein rather than simple BCL-2-epitope destruction. Photochemical damage was not observed in Bcl-x(L), Bax, Bad, the voltage-dependent anion channel, or the adenine nucleotide translocator. Therefore, Bcl-2 is one target of PDT with Pc 4, and PDT damage to Bcl-2 contributes to its efficient induction of apoptosis. |
| doi_str_mv | 10.1038/sj.onc.1204441 |
| format | Article |
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With the mitochondrial photosensitizer Pc 4, PDT induces apoptosis, yet its molecular targets are not known. Here, the anti-apoptotic protein Bcl-2 is shown to be highly sensitive to PDT, as judged on Western blots by the disappearance of anti-Bcl-2-reactive material from the position of the native 26 kDa protein. The loss of Bcl-2 was PDT dose dependent and was observed for both endogenous and overexpressed Bcl-2 in several cell lines, immediately after PDT, and with chilled cells. It was accompanied by a trace of a 23-kDa cleavage product as well as high-molecular weight products that may result from photochemical crosslinking. PDT-induced Bcl-2 loss occurred in MCF-7 cells that do not express caspase-3 or in the presence of protease inhibitors, but was prevented, along with the induction of apoptosis, by the singlet oxygen scavenger L-histidine. Loss of FLAG-Bcl-2 was observed with both anti-FLAG and anti-Bcl-2 antibodies, indicating loss of native protein rather than simple BCL-2-epitope destruction. Photochemical damage was not observed in Bcl-x(L), Bax, Bad, the voltage-dependent anion channel, or the adenine nucleotide translocator. Therefore, Bcl-2 is one target of PDT with Pc 4, and PDT damage to Bcl-2 contributes to its efficient induction of apoptosis.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1204441</identifier><identifier>PMID: 11423992</identifier><identifier>CODEN: ONCNES</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing</publisher><subject>Active oxygen ; Adenine ; Adenocarcinoma - pathology ; Animals ; Antigenic determinants ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - radiation effects ; Bax protein ; Bcl-2 protein ; Bcl-x protein ; Biological and medical sciences ; Breast Neoplasms - pathology ; Carcinoma, Squamous Cell - pathology ; Caspase-3 ; Cell lines ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Chemical properties ; CHO Cells - drug effects ; CHO Cells - radiation effects ; Cricetinae ; Cricetulus ; Crosslinked polymers ; Drug dosages ; Dyes and dyeing ; Epitopes ; Female ; Free Radical Scavengers - pharmacology ; Fundamental and applied biological sciences. Psychology ; Histidine ; Histidine - pharmacology ; Humans ; Indoles - pharmacology ; Indoles - therapeutic use ; Intracellular Membranes - chemistry ; Intracellular Membranes - drug effects ; Intracellular Membranes - radiation effects ; Laryngeal Neoplasms - pathology ; Male ; Membrane Proteins - drug effects ; Membrane Proteins - radiation effects ; Mitochondria ; Molecular and cellular biology ; Molecular Weight ; Neoplasm Proteins - drug effects ; Neoplasm Proteins - radiation effects ; Oncology ; Oncoproteins ; Oxidative Stress ; Oxygen - metabolism ; Photochemistry ; Photochemotherapy ; Photodynamic therapy ; Photosensitizing Agents - pharmacology ; Photosensitizing Agents - therapeutic use ; Phthalocyanins ; Prostatic Neoplasms - pathology ; Protease inhibitors ; Proteases ; Proteinase inhibitors ; Proteins ; Proto-Oncogene Proteins c-bcl-2 - drug effects ; Proto-Oncogene Proteins c-bcl-2 - radiation effects ; Radiation ; Singlet Oxygen ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - radiation effects ; Tumors ; Western blotting</subject><ispartof>Oncogene, 2001-06, Vol.20 (26), p.3420-3427</ispartof><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2001 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 7, 2001</rights><rights>Macmillan Publishers Limited 2001.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-6546cbe2758b675ad05dcb91fb2fe1d0a85bd1d7d4796eac9ea21ff59a182d1c3</citedby><cites>FETCH-LOGICAL-c486t-6546cbe2758b675ad05dcb91fb2fe1d0a85bd1d7d4796eac9ea21ff59a182d1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14087624$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11423992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>XUE, Liang-Yan</creatorcontrib><creatorcontrib>CHIU, Song-Mao</creatorcontrib><creatorcontrib>OLEINICK, Nancy L</creatorcontrib><title>Photochemical destruction of the Bcl-2 oncoprotein during photodynamic therapy with the phthalocyanine photosensitizer Pc 4</title><title>Oncogene</title><addtitle>Oncogene</addtitle><description>Photodynamic therapy (PDT), utilizing a photosensitizer and visible light, causes localized oxidative damage. With the mitochondrial photosensitizer Pc 4, PDT induces apoptosis, yet its molecular targets are not known. Here, the anti-apoptotic protein Bcl-2 is shown to be highly sensitive to PDT, as judged on Western blots by the disappearance of anti-Bcl-2-reactive material from the position of the native 26 kDa protein. The loss of Bcl-2 was PDT dose dependent and was observed for both endogenous and overexpressed Bcl-2 in several cell lines, immediately after PDT, and with chilled cells. It was accompanied by a trace of a 23-kDa cleavage product as well as high-molecular weight products that may result from photochemical crosslinking. PDT-induced Bcl-2 loss occurred in MCF-7 cells that do not express caspase-3 or in the presence of protease inhibitors, but was prevented, along with the induction of apoptosis, by the singlet oxygen scavenger L-histidine. Loss of FLAG-Bcl-2 was observed with both anti-FLAG and anti-Bcl-2 antibodies, indicating loss of native protein rather than simple BCL-2-epitope destruction. Photochemical damage was not observed in Bcl-x(L), Bax, Bad, the voltage-dependent anion channel, or the adenine nucleotide translocator. Therefore, Bcl-2 is one target of PDT with Pc 4, and PDT damage to Bcl-2 contributes to its efficient induction of apoptosis.</description><subject>Active oxygen</subject><subject>Adenine</subject><subject>Adenocarcinoma - pathology</subject><subject>Animals</subject><subject>Antigenic determinants</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - radiation effects</subject><subject>Bax protein</subject><subject>Bcl-2 protein</subject><subject>Bcl-x protein</subject><subject>Biological and medical sciences</subject><subject>Breast Neoplasms - pathology</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Caspase-3</subject><subject>Cell lines</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Chemical properties</subject><subject>CHO Cells - drug effects</subject><subject>CHO Cells - radiation effects</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Crosslinked polymers</subject><subject>Drug dosages</subject><subject>Dyes and dyeing</subject><subject>Epitopes</subject><subject>Female</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histidine</subject><subject>Histidine - pharmacology</subject><subject>Humans</subject><subject>Indoles - pharmacology</subject><subject>Indoles - therapeutic use</subject><subject>Intracellular Membranes - chemistry</subject><subject>Intracellular Membranes - drug effects</subject><subject>Intracellular Membranes - radiation effects</subject><subject>Laryngeal Neoplasms - pathology</subject><subject>Male</subject><subject>Membrane Proteins - drug effects</subject><subject>Membrane Proteins - radiation effects</subject><subject>Mitochondria</subject><subject>Molecular and cellular biology</subject><subject>Molecular Weight</subject><subject>Neoplasm Proteins - drug effects</subject><subject>Neoplasm Proteins - radiation effects</subject><subject>Oncology</subject><subject>Oncoproteins</subject><subject>Oxidative Stress</subject><subject>Oxygen - metabolism</subject><subject>Photochemistry</subject><subject>Photochemotherapy</subject><subject>Photodynamic therapy</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Photosensitizing Agents - therapeutic use</subject><subject>Phthalocyanins</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Protease inhibitors</subject><subject>Proteases</subject><subject>Proteinase inhibitors</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-bcl-2 - drug effects</subject><subject>Proto-Oncogene Proteins c-bcl-2 - radiation effects</subject><subject>Radiation</subject><subject>Singlet Oxygen</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - radiation effects</subject><subject>Tumors</subject><subject>Western blotting</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFks2LFDEQxYMo7rh69SiNst56TKWTTue4LusHLLgHPTfpfGxn6EnaJM0y-s-bcRoXBJEcAuH3KlX1HkIvAW8BN927tNsGr7ZAMKUUHqENUN7WjAn6GG2wYLgWpCFn6FlKO4wxF5g8RWcAlDRCkA36eTuGHNRo9k7JqdIm5bio7IKvgq3yaKr3aqpJVT4JcwzZOF_pJTp_V81HpT54WaRHMsr5UN27PP6WzWMe5RTUQXrnzQlOxieX3Q8Tq1tV0efoiZVTMi_W-xx9-3D99epTffPl4-ery5ta0a7NdctoqwZDOOuGljOpMdNqEGAHYg1oLDs2aNBcUy5aI5UwkoC1TEjoiAbVnKO3p7plgO9LmbDfu6TMNElvwpJ6XvbESQP_BaGsDyhuCvjmL3AXlujLED1pKTQAHFihXv-TIrzBhBFcoO0JupOT6Z23IUepytFHS4I31pX3S4JZW0oX2_4IVAwpRWP7Obq9jIcecH_MRJ92fbGrXzNRBK_WNpZhb_QDvoagABcrIFMJgY3SK5ceOIo73hLa_AJQUMDV</recordid><startdate>20010607</startdate><enddate>20010607</enddate><creator>XUE, Liang-Yan</creator><creator>CHIU, Song-Mao</creator><creator>OLEINICK, Nancy L</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010607</creationdate><title>Photochemical destruction of the Bcl-2 oncoprotein during photodynamic therapy with the phthalocyanine photosensitizer Pc 4</title><author>XUE, Liang-Yan ; CHIU, Song-Mao ; OLEINICK, Nancy L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-6546cbe2758b675ad05dcb91fb2fe1d0a85bd1d7d4796eac9ea21ff59a182d1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Active oxygen</topic><topic>Adenine</topic><topic>Adenocarcinoma - pathology</topic><topic>Animals</topic><topic>Antigenic determinants</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - radiation effects</topic><topic>Bax protein</topic><topic>Bcl-2 protein</topic><topic>Bcl-x protein</topic><topic>Biological and medical sciences</topic><topic>Breast Neoplasms - pathology</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Caspase-3</topic><topic>Cell lines</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Chemical properties</topic><topic>CHO Cells - drug effects</topic><topic>CHO Cells - radiation effects</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Crosslinked polymers</topic><topic>Drug dosages</topic><topic>Dyes and dyeing</topic><topic>Epitopes</topic><topic>Female</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histidine</topic><topic>Histidine - pharmacology</topic><topic>Humans</topic><topic>Indoles - pharmacology</topic><topic>Indoles - therapeutic use</topic><topic>Intracellular Membranes - chemistry</topic><topic>Intracellular Membranes - drug effects</topic><topic>Intracellular Membranes - radiation effects</topic><topic>Laryngeal Neoplasms - pathology</topic><topic>Male</topic><topic>Membrane Proteins - drug effects</topic><topic>Membrane Proteins - radiation effects</topic><topic>Mitochondria</topic><topic>Molecular and cellular biology</topic><topic>Molecular Weight</topic><topic>Neoplasm Proteins - drug effects</topic><topic>Neoplasm Proteins - radiation effects</topic><topic>Oncology</topic><topic>Oncoproteins</topic><topic>Oxidative Stress</topic><topic>Oxygen - metabolism</topic><topic>Photochemistry</topic><topic>Photochemotherapy</topic><topic>Photodynamic therapy</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Photosensitizing Agents - therapeutic use</topic><topic>Phthalocyanins</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Protease inhibitors</topic><topic>Proteases</topic><topic>Proteinase inhibitors</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-bcl-2 - drug effects</topic><topic>Proto-Oncogene Proteins c-bcl-2 - radiation effects</topic><topic>Radiation</topic><topic>Singlet Oxygen</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - radiation effects</topic><topic>Tumors</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>XUE, Liang-Yan</creatorcontrib><creatorcontrib>CHIU, Song-Mao</creatorcontrib><creatorcontrib>OLEINICK, Nancy L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>XUE, Liang-Yan</au><au>CHIU, Song-Mao</au><au>OLEINICK, Nancy L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photochemical destruction of the Bcl-2 oncoprotein during photodynamic therapy with the phthalocyanine photosensitizer Pc 4</atitle><jtitle>Oncogene</jtitle><addtitle>Oncogene</addtitle><date>2001-06-07</date><risdate>2001</risdate><volume>20</volume><issue>26</issue><spage>3420</spage><epage>3427</epage><pages>3420-3427</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><coden>ONCNES</coden><abstract>Photodynamic therapy (PDT), utilizing a photosensitizer and visible light, causes localized oxidative damage. With the mitochondrial photosensitizer Pc 4, PDT induces apoptosis, yet its molecular targets are not known. Here, the anti-apoptotic protein Bcl-2 is shown to be highly sensitive to PDT, as judged on Western blots by the disappearance of anti-Bcl-2-reactive material from the position of the native 26 kDa protein. The loss of Bcl-2 was PDT dose dependent and was observed for both endogenous and overexpressed Bcl-2 in several cell lines, immediately after PDT, and with chilled cells. It was accompanied by a trace of a 23-kDa cleavage product as well as high-molecular weight products that may result from photochemical crosslinking. PDT-induced Bcl-2 loss occurred in MCF-7 cells that do not express caspase-3 or in the presence of protease inhibitors, but was prevented, along with the induction of apoptosis, by the singlet oxygen scavenger L-histidine. Loss of FLAG-Bcl-2 was observed with both anti-FLAG and anti-Bcl-2 antibodies, indicating loss of native protein rather than simple BCL-2-epitope destruction. Photochemical damage was not observed in Bcl-x(L), Bax, Bad, the voltage-dependent anion channel, or the adenine nucleotide translocator. Therefore, Bcl-2 is one target of PDT with Pc 4, and PDT damage to Bcl-2 contributes to its efficient induction of apoptosis.</abstract><cop>Basingstoke</cop><pub>Nature Publishing</pub><pmid>11423992</pmid><doi>10.1038/sj.onc.1204441</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | Active oxygen Adenine Adenocarcinoma - pathology Animals Antigenic determinants Apoptosis Apoptosis - drug effects Apoptosis - radiation effects Bax protein Bcl-2 protein Bcl-x protein Biological and medical sciences Breast Neoplasms - pathology Carcinoma, Squamous Cell - pathology Caspase-3 Cell lines Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Chemical properties CHO Cells - drug effects CHO Cells - radiation effects Cricetinae Cricetulus Crosslinked polymers Drug dosages Dyes and dyeing Epitopes Female Free Radical Scavengers - pharmacology Fundamental and applied biological sciences. Psychology Histidine Histidine - pharmacology Humans Indoles - pharmacology Indoles - therapeutic use Intracellular Membranes - chemistry Intracellular Membranes - drug effects Intracellular Membranes - radiation effects Laryngeal Neoplasms - pathology Male Membrane Proteins - drug effects Membrane Proteins - radiation effects Mitochondria Molecular and cellular biology Molecular Weight Neoplasm Proteins - drug effects Neoplasm Proteins - radiation effects Oncology Oncoproteins Oxidative Stress Oxygen - metabolism Photochemistry Photochemotherapy Photodynamic therapy Photosensitizing Agents - pharmacology Photosensitizing Agents - therapeutic use Phthalocyanins Prostatic Neoplasms - pathology Protease inhibitors Proteases Proteinase inhibitors Proteins Proto-Oncogene Proteins c-bcl-2 - drug effects Proto-Oncogene Proteins c-bcl-2 - radiation effects Radiation Singlet Oxygen Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - radiation effects Tumors Western blotting |
| title | Photochemical destruction of the Bcl-2 oncoprotein during photodynamic therapy with the phthalocyanine photosensitizer Pc 4 |
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