A novel EBNA-1 tag system for high level expression and efficient detection of fusion proteins in vitro and in vivo

A highly specific monoclonal antibody, which recognises an epitope between amino acids (aa) 408 and 446 of EBNA-1, was found to immunoprecipitate this protein with great efficiency. By adding a 39-aa tag, the BGLF4 protein product of EBV was shown to express in the cytoplasm of transfected cells. Th...

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Veröffentlicht in:	Journal of virological methods 2000-03, Vol.85 (1), p.35-41
Hauptverfasser:	Chen, Mei-Ru, Huang, Hsiaowen, Fen, Chia-Yu, Chen, Jen-Yang
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal BGLF4 protein Biological and medical sciences Black beetle virus Cell Line EBNA-1 tag EBNA1 protein EBV BGLF4 Epitopes Epstein-Barr virus Epstein-Barr Virus Nuclear Antigens - immunology Epstein-Barr Virus Nuclear Antigens - metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene expression Genetic Vectors Humans Immunoblotting Immunoprecipitation Microbiology Precipitin Tests Promoter Regions, Genetic Protein Biosynthesis Protein-Serine-Threonine Kinases - metabolism Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - metabolism Simian virus 40 Techniques used in virology Transcription, Genetic Transfection Viral Proteins Virology
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