High serum folates and the simplification of red cell folate analysis
Recent substantial increases in clinical blood folate concentrations are noted. Since red cell folates (RCF) are calculated from whole blood folates (WBF) by subtraction of the endogenous serum folate (SF) component, the reporting of clinical RCF results may be delayed because an ever increasing pro...
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| Veröffentlicht in: | Clinical and laboratory haematology 2001-02, Vol.23 (1), p.15-20 |
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| creator | Philpott, N. Kelleher, B.P. Smith, O.P. O'Broin, S.D. |
| description | Recent substantial increases in clinical blood folate concentrations are noted. Since red cell folates (RCF) are calculated from whole blood folates (WBF) by subtraction of the endogenous serum folate (SF) component, the reporting of clinical RCF results may be delayed because an ever increasing proportion (15%) of diagnostic SF levels are high (> 20 ng/ml) and need a repeat analysis.
We evaluated ‘plasma replacement’ as a simple preanalytical procedure in which endogenous blood plasma is removed from red cells by washing and substituted with ‘low‐folate’ plasma (serum) as an alternative conjugase (gamma‐glutamyl carboxypeptidase) source for folate polyglutamate hydrolysis. Washed and conventional RCF assays compared well after both manual (n=115, r=0.98, y=1x + 1.26) and automated washing of red cells (n=170, r=0.96, y=0.96x – 0.73 ng/ml) and were not significantly different. The interassay reproducibility of folate results from washed blood samples was good (CV= |
| doi_str_mv | 10.1046/j.1365-2257.2001.00355.x |
| format | Article |
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We evaluated ‘plasma replacement’ as a simple preanalytical procedure in which endogenous blood plasma is removed from red cells by washing and substituted with ‘low‐folate’ plasma (serum) as an alternative conjugase (gamma‐glutamyl carboxypeptidase) source for folate polyglutamate hydrolysis. Washed and conventional RCF assays compared well after both manual (n=115, r=0.98, y=1x + 1.26) and automated washing of red cells (n=170, r=0.96, y=0.96x – 0.73 ng/ml) and were not significantly different. The interassay reproducibility of folate results from washed blood samples was good (CV=< 6%).
This novel ‘plasma replacement’ step halves the cost of a valid RCF assay by eliminating the need for endogenous SF analysis, and it expedites the reporting of clinical results.</description><identifier>ISSN: 0141-9854</identifier><identifier>EISSN: 1365-2257</identifier><identifier>DOI: 10.1046/j.1365-2257.2001.00355.x</identifier><identifier>PMID: 11422225</identifier><identifier>CODEN: CLHAD3</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Clinical Laboratory Techniques - standards ; Erythrocytes - chemistry ; Folic Acid - blood ; Folic Acid - standards ; Folic Acid Deficiency - blood ; Folic Acid Deficiency - diagnosis ; haemoglobin folate ; Hematology ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Methods ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Red cell folate ; Reproducibility of Results ; serum folate ; Time Factors</subject><ispartof>Clinical and laboratory haematology, 2001-02, Vol.23 (1), p.15-20</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3835-b29d65c8a565cc2e6611db2ab4e9618836d9d299ba71484fe7f3db64c67cbcfc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2257.2001.00355.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2257.2001.00355.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1123475$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11422225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Philpott, N.</creatorcontrib><creatorcontrib>Kelleher, B.P.</creatorcontrib><creatorcontrib>Smith, O.P.</creatorcontrib><creatorcontrib>O'Broin, S.D.</creatorcontrib><title>High serum folates and the simplification of red cell folate analysis</title><title>Clinical and laboratory haematology</title><addtitle>Clin Lab Haematol</addtitle><description>Recent substantial increases in clinical blood folate concentrations are noted. Since red cell folates (RCF) are calculated from whole blood folates (WBF) by subtraction of the endogenous serum folate (SF) component, the reporting of clinical RCF results may be delayed because an ever increasing proportion (15%) of diagnostic SF levels are high (> 20 ng/ml) and need a repeat analysis.
We evaluated ‘plasma replacement’ as a simple preanalytical procedure in which endogenous blood plasma is removed from red cells by washing and substituted with ‘low‐folate’ plasma (serum) as an alternative conjugase (gamma‐glutamyl carboxypeptidase) source for folate polyglutamate hydrolysis. Washed and conventional RCF assays compared well after both manual (n=115, r=0.98, y=1x + 1.26) and automated washing of red cells (n=170, r=0.96, y=0.96x – 0.73 ng/ml) and were not significantly different. The interassay reproducibility of folate results from washed blood samples was good (CV=< 6%).
This novel ‘plasma replacement’ step halves the cost of a valid RCF assay by eliminating the need for endogenous SF analysis, and it expedites the reporting of clinical results.</description><subject>Biological and medical sciences</subject><subject>Clinical Laboratory Techniques - standards</subject><subject>Erythrocytes - chemistry</subject><subject>Folic Acid - blood</subject><subject>Folic Acid - standards</subject><subject>Folic Acid Deficiency - blood</subject><subject>Folic Acid Deficiency - diagnosis</subject><subject>haemoglobin folate</subject><subject>Hematology</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Red cell folate</subject><subject>Reproducibility of Results</subject><subject>serum folate</subject><subject>Time Factors</subject><issn>0141-9854</issn><issn>1365-2257</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkUlPwzAQhS0EgrL8BeQD4pbg3fGBA0KFFiKQ2Hq0HMcBl6QpcSraf09Cy-LD2NL73sgzDwCIUYwRE2fTGFPBI0K4jAlCOEaIch4vt8DgV9gGA4QZjlTC2R7YD2HagRRLuQv2MGakO3wAhiP_-gaDaxYVLOrStC5AM8th--Zg8NW89IW3pvX1DNYFbFwOrSvLDdqRplwFHw7BTmHK4I429wF4vho-XY6i9P56fHmRRpYmlEcZUbngNjG8q5Y4ITDOM2Iy5pTASUJFrnKiVGYkZgkrnCxonglmhbSZLSw9AKfrvvOm_li40OrKh_5DZubqRdASKU6JYh14vAEXWeVyPW98ZZqV_hm8A042gAnWlEVjZtaHfxyhTPbY-Rr79KVb_clI9znoqe7Xrft16z4H_Z2DXurxTTrqXp0_Wvt9aN3y12-ady0klVxP7q71y4OaPOI01bf0C8_Zigk</recordid><startdate>200102</startdate><enddate>200102</enddate><creator>Philpott, N.</creator><creator>Kelleher, B.P.</creator><creator>Smith, O.P.</creator><creator>O'Broin, S.D.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200102</creationdate><title>High serum folates and the simplification of red cell folate analysis</title><author>Philpott, N. ; Kelleher, B.P. ; Smith, O.P. ; O'Broin, S.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3835-b29d65c8a565cc2e6611db2ab4e9618836d9d299ba71484fe7f3db64c67cbcfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>Clinical Laboratory Techniques - standards</topic><topic>Erythrocytes - chemistry</topic><topic>Folic Acid - blood</topic><topic>Folic Acid - standards</topic><topic>Folic Acid Deficiency - blood</topic><topic>Folic Acid Deficiency - diagnosis</topic><topic>haemoglobin folate</topic><topic>Hematology</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Red cell folate</topic><topic>Reproducibility of Results</topic><topic>serum folate</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Philpott, N.</creatorcontrib><creatorcontrib>Kelleher, B.P.</creatorcontrib><creatorcontrib>Smith, O.P.</creatorcontrib><creatorcontrib>O'Broin, S.D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and laboratory haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Philpott, N.</au><au>Kelleher, B.P.</au><au>Smith, O.P.</au><au>O'Broin, S.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High serum folates and the simplification of red cell folate analysis</atitle><jtitle>Clinical and laboratory haematology</jtitle><addtitle>Clin Lab Haematol</addtitle><date>2001-02</date><risdate>2001</risdate><volume>23</volume><issue>1</issue><spage>15</spage><epage>20</epage><pages>15-20</pages><issn>0141-9854</issn><eissn>1365-2257</eissn><coden>CLHAD3</coden><abstract>Recent substantial increases in clinical blood folate concentrations are noted. Since red cell folates (RCF) are calculated from whole blood folates (WBF) by subtraction of the endogenous serum folate (SF) component, the reporting of clinical RCF results may be delayed because an ever increasing proportion (15%) of diagnostic SF levels are high (> 20 ng/ml) and need a repeat analysis.
We evaluated ‘plasma replacement’ as a simple preanalytical procedure in which endogenous blood plasma is removed from red cells by washing and substituted with ‘low‐folate’ plasma (serum) as an alternative conjugase (gamma‐glutamyl carboxypeptidase) source for folate polyglutamate hydrolysis. Washed and conventional RCF assays compared well after both manual (n=115, r=0.98, y=1x + 1.26) and automated washing of red cells (n=170, r=0.96, y=0.96x – 0.73 ng/ml) and were not significantly different. The interassay reproducibility of folate results from washed blood samples was good (CV=< 6%).
This novel ‘plasma replacement’ step halves the cost of a valid RCF assay by eliminating the need for endogenous SF analysis, and it expedites the reporting of clinical results.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>11422225</pmid><doi>10.1046/j.1365-2257.2001.00355.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Clinical and laboratory haematology, 2001-02, Vol.23 (1), p.15-20 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Clinical Laboratory Techniques - standards Erythrocytes - chemistry Folic Acid - blood Folic Acid - standards Folic Acid Deficiency - blood Folic Acid Deficiency - diagnosis haemoglobin folate Hematology Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Methods Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Red cell folate Reproducibility of Results serum folate Time Factors |
| title | High serum folates and the simplification of red cell folate analysis |
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