A group I intron in the nuclear small subunit ribosomal DNA of Gaeumannomyces graminis
The length of the small subunit ribosomal DNA (SSU rDNA) differs among isolates of species and varieties of Gaeumannomyces. The sequence of the 3' region of the SSU rDNA revealed 340-, 365-, and 520-bp insertions for G. graminis varieties avenae, tritici, and graminis, respectively. The intron...
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| Veröffentlicht in: | Current microbiology 2000-05, Vol.40 (5), p.291-296 |
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| description | The length of the small subunit ribosomal DNA (SSU rDNA) differs among isolates of species and varieties of Gaeumannomyces. The sequence of the 3' region of the SSU rDNA revealed 340-, 365-, and 520-bp insertions for G. graminis varieties avenae, tritici, and graminis, respectively. The intron sequences from varities tritici and avenae were similar, except there was an insert of 23 nucleotides at base 328 from the 5' end of the G. g. var. tritici intron. The G. g. var. graminis intron sequences had 92.4% homology compared with the intron sequences of varieties tritici and avenae. In addition, the intron sequence of variety graminis is larger, having an insert of 155 nucleotides at base 365 of the 5' end of the intron. Little variation in the DNA sequences flanking the introns has been detected among the isolates of Gaeumannomyces that either have or lack an intron. Reverse transcriptase PCR (RT-PCR) indicated the absence of the intron in the mature rRNA. The intron sequence had both a conserved sequence and secondary structural elements classifying it as a group I intron. |
| doi_str_mv | 10.1007/s002849910058 |
| format | Article |
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M ; WILKINSON, H. T</creator><creatorcontrib>FOULY, H. M ; WILKINSON, H. T</creatorcontrib><description>The length of the small subunit ribosomal DNA (SSU rDNA) differs among isolates of species and varieties of Gaeumannomyces. The sequence of the 3' region of the SSU rDNA revealed 340-, 365-, and 520-bp insertions for G. graminis varieties avenae, tritici, and graminis, respectively. The intron sequences from varities tritici and avenae were similar, except there was an insert of 23 nucleotides at base 328 from the 5' end of the G. g. var. tritici intron. The G. g. var. graminis intron sequences had 92.4% homology compared with the intron sequences of varieties tritici and avenae. In addition, the intron sequence of variety graminis is larger, having an insert of 155 nucleotides at base 365 of the 5' end of the intron. Little variation in the DNA sequences flanking the introns has been detected among the isolates of Gaeumannomyces that either have or lack an intron. Reverse transcriptase PCR (RT-PCR) indicated the absence of the intron in the mature rRNA. The intron sequence had both a conserved sequence and secondary structural elements classifying it as a group I intron.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002849910058</identifier><identifier>PMID: 10706657</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Ascomycota - genetics ; Base Sequence ; Biological and medical sciences ; Conserved sequence ; Deoxyribonucleic acid ; DNA ; DNA, Fungal - genetics ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Gaeumannomyces ; Genetic Variation ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Introns - genetics ; Microbiology ; Molecular Sequence Data ; Mycology ; Nucleic Acid Conformation ; Phytopathology. 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Plant and forest protection ; Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Ribosomal - chemistry ; RNA, Ribosomal - genetics ; Sequence Analysis, DNA ; Variation, races, biotypes, parasitic specialization, genetics</subject><ispartof>Current microbiology, 2000-05, Vol.40 (5), p.291-296</ispartof><rights>2000 INIST-CNRS</rights><rights>Springer-Verlag New York Inc. 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-71aa156345b16d9e6e3c1d7452fbfe02ae978db6fef62d25c48e3f8cfe2062ff3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1464831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10706657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FOULY, H. M</creatorcontrib><creatorcontrib>WILKINSON, H. T</creatorcontrib><title>A group I intron in the nuclear small subunit ribosomal DNA of Gaeumannomyces graminis</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>The length of the small subunit ribosomal DNA (SSU rDNA) differs among isolates of species and varieties of Gaeumannomyces. The sequence of the 3' region of the SSU rDNA revealed 340-, 365-, and 520-bp insertions for G. graminis varieties avenae, tritici, and graminis, respectively. The intron sequences from varities tritici and avenae were similar, except there was an insert of 23 nucleotides at base 328 from the 5' end of the G. g. var. tritici intron. The G. g. var. graminis intron sequences had 92.4% homology compared with the intron sequences of varieties tritici and avenae. In addition, the intron sequence of variety graminis is larger, having an insert of 155 nucleotides at base 365 of the 5' end of the intron. Little variation in the DNA sequences flanking the introns has been detected among the isolates of Gaeumannomyces that either have or lack an intron. Reverse transcriptase PCR (RT-PCR) indicated the absence of the intron in the mature rRNA. The intron sequence had both a conserved sequence and secondary structural elements classifying it as a group I intron.</description><subject>Ascomycota - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Conserved sequence</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Gaeumannomyces</subject><subject>Genetic Variation</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Introns - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycology</subject><subject>Nucleic Acid Conformation</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Ribosomal - chemistry</subject><subject>RNA, Ribosomal - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Variation, races, biotypes, parasitic specialization, genetics</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0U2LFDEQBuAgijuuHr1KwEW8tFa-08dh1XVh0Yt6bdLpivbSnYxJ57D_3sgM-HHQU1HFwwvFS8hTBq8YgHldALiVfd8WZe-RHZOCd9D2-2QHQorOasXOyKNSbgEY74E9JGcMDGitzI582dOvOdUDvaZz3HKKbdDtG9JY_YIu07K6ZaGljjXOG83zmEpqJ_rmw56mQK8c1tXFmNY7j6VluXWOc3lMHgS3FHxymufk87u3ny7fdzcfr64v9zedlwy2zjDnmNJCqpHpqUeNwrPJSMXDGBC4w97YadQBg-YTV15aFMH6gBw0D0GckxfH3ENO3yuWbVjn4nFZXMRUy2CgV8CB_RcyKy1XQjf48t9QNghGy77R53_R21RzbP82JVhvhTaiqe6ofE6lZAzDIc-ry3cDg-FnhcMfFTb_7JRaxxWn3_SxswYuTsAV75aQXfRz-eWkllYw8QOwJ6Fd</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>FOULY, H. 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M ; WILKINSON, H. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-71aa156345b16d9e6e3c1d7452fbfe02ae978db6fef62d25c48e3f8cfe2062ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Ascomycota - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Conserved sequence</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Gaeumannomyces</topic><topic>Genetic Variation</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Introns - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycology</topic><topic>Nucleic Acid Conformation</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Polymerase Chain Reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Ribosomal - chemistry</topic><topic>RNA, Ribosomal - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Variation, races, biotypes, parasitic specialization, genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FOULY, H. M</creatorcontrib><creatorcontrib>WILKINSON, H. 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M</au><au>WILKINSON, H. T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A group I intron in the nuclear small subunit ribosomal DNA of Gaeumannomyces graminis</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>40</volume><issue>5</issue><spage>291</spage><epage>296</epage><pages>291-296</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The length of the small subunit ribosomal DNA (SSU rDNA) differs among isolates of species and varieties of Gaeumannomyces. The sequence of the 3' region of the SSU rDNA revealed 340-, 365-, and 520-bp insertions for G. graminis varieties avenae, tritici, and graminis, respectively. The intron sequences from varities tritici and avenae were similar, except there was an insert of 23 nucleotides at base 328 from the 5' end of the G. g. var. tritici intron. The G. g. var. graminis intron sequences had 92.4% homology compared with the intron sequences of varieties tritici and avenae. In addition, the intron sequence of variety graminis is larger, having an insert of 155 nucleotides at base 365 of the 5' end of the intron. Little variation in the DNA sequences flanking the introns has been detected among the isolates of Gaeumannomyces that either have or lack an intron. Reverse transcriptase PCR (RT-PCR) indicated the absence of the intron in the mature rRNA. The intron sequence had both a conserved sequence and secondary structural elements classifying it as a group I intron.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>10706657</pmid><doi>10.1007/s002849910058</doi><tpages>6</tpages></addata></record> |
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| ispartof | Current microbiology, 2000-05, Vol.40 (5), p.291-296 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Ascomycota - genetics Base Sequence Biological and medical sciences Conserved sequence Deoxyribonucleic acid DNA DNA, Fungal - genetics DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Fungal plant pathogens Gaeumannomyces Genetic Variation Growth, nutrition, metabolism, transports, enzymes. Molecular biology Introns - genetics Microbiology Molecular Sequence Data Mycology Nucleic Acid Conformation Phytopathology. Animal pests. Plant and forest protection Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Ribosomal - chemistry RNA, Ribosomal - genetics Sequence Analysis, DNA Variation, races, biotypes, parasitic specialization, genetics |
| title | A group I intron in the nuclear small subunit ribosomal DNA of Gaeumannomyces graminis |
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